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  1. Article ; Online: Neonatal point-of-care lung ultrasound: what should be known and done out of the NICU?

    Alonso-Ojembarrena, Almudena / Gregorio-Hernández, Rebeca / Raimondi, Francesco

    European journal of pediatrics

    2024  Volume 183, Issue 4, Page(s) 1555–1565

    Abstract: Lung ultrasound is rapidly becoming a useful tool in the care of neonates: its ease of use, reproducibility, low cost, and negligible side effects make it a very suitable tool for the respiratory care of all neonates. This technique has been extensively ... ...

    Abstract Lung ultrasound is rapidly becoming a useful tool in the care of neonates: its ease of use, reproducibility, low cost, and negligible side effects make it a very suitable tool for the respiratory care of all neonates. This technique has been extensively studied by different approaches in neonatal intensive care unit (NICU), both for diagnostic and prognostic aims and to guide respiratory treatments. However, many neonates are being born in level I/II hospitals without NICU facilities so all pediatricians, not just neonatal intensivists, should be aware of its potential. This is made possible by the increasing access to ultrasound machines in a modern hospital setting. In this review, we describe the ultrasonographic characteristics of the normal neonatal lung. We also discuss the ultrasound features of main neonatal respiratory diseases: transient tachypnea of the neonate (TTN), respiratory distress syndrome (RDS), meconium aspiration syndrome (MAS), pneumothorax (PNX), pleural effusion (PE), or pneumonia. Finally, we mention two functional approaches to lung ultrasound: 1. The use of lung ultrasound in level I delivery centers as a mean to assess the severity of neonatal respiratory distress and request a transport to a higher degree structure in a timely fashion. 2. The prognostic accuracy of lung ultrasound for early and targeted surfactant replacement.
    Conclusion:  LU is still a useful tool in level I/II neonatal units, both for diagnostic and functional issues.
    What is known: • Neonatal lung ultrasound has been recently introduced in the usual care in many Neonatal Intensive Care Units.
    What is new: • It also has many advantages in level I/II neonatal units, both for neonatologist or even pediatricians that treat neonates in those sites.
    MeSH term(s) Female ; Humans ; Infant, Newborn ; Intensive Care Units, Neonatal ; Reproducibility of Results ; Point-of-Care Systems ; Meconium Aspiration Syndrome ; Lung/diagnostic imaging ; Respiratory Distress Syndrome, Newborn/diagnostic imaging ; Respiratory Distress Syndrome, Newborn/therapy ; Pneumonia ; Infant, Newborn, Diseases ; Ultrasonography
    Language English
    Publishing date 2024-02-05
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-023-05375-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Reply to: Lung ultrasound in neonates with congenital diaphragmatic hernia and the need for a quantitative evaluation.

    Gregorio-Hernández, R / Sánchez-Luna, M

    European journal of pediatrics

    2023  Volume 182, Issue 12, Page(s) 5715–5716

    MeSH term(s) Infant, Newborn ; Humans ; Hernias, Diaphragmatic, Congenital/diagnostic imaging ; Lung/diagnostic imaging ; Thorax ; Ultrasonography
    Language English
    Publishing date 2023-10-17
    Publishing country Germany
    Document type Letter ; Comment
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-023-05256-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A case of neonatal tuberous sclerosis diagnosed by lung ultrasound.

    Gregorio-Hernández, Rebeca / Chimenti-Camacho, Paz / Aguado Del Hoyo, Alejandra / Sánchez-Luna, Manuel

    Anales de pediatria

    2023  Volume 99, Issue 1, Page(s) 71–73

    MeSH term(s) Infant, Newborn ; Humans ; Tuberous Sclerosis/complications ; Tuberous Sclerosis/diagnostic imaging ; Lung/diagnostic imaging ; Ultrasonography ; Thorax
    Language English
    Publishing date 2023-06-08
    Publishing country Spain
    Document type Case Reports ; Journal Article
    ZDB-ID 2830901-7
    ISSN 2341-2879 ; 2341-2879
    ISSN (online) 2341-2879
    ISSN 2341-2879
    DOI 10.1016/j.anpede.2023.06.006
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  4. Article ; Online: Isotopologue profiling of triterpene formation under physiological conditions. Biosynthesis of lupeol-3-(3'-R-hydroxy)-stearate in Pentalinon andrieuxii.

    Peña-Rodríguez, Luis M / Yam-Puc, Alejandro / Knispel, Nihat / Schramek, Nicholas / Huber, Claudia / Graßberger, Christoph / Ramírez-Torres, Fabiola G / Escalante-Erosa, Fabiola / García-Sosa, Karlina / Hiebert-Giesbrecht, Mickel R / Chan-Bacab, Manuel J / Godoy-Hernández, Gregorio / Bacher, Adelbert / Eisenreich, Wolfgang

    The Journal of organic chemistry

    2014  Volume 79, Issue 7, Page(s) 2864–2873

    Abstract: The biosynthesis of lupeol-3-(3'R-hydroxy)-stearate (procrim b, 1) was investigated in the Mexican medicinal plant Pentalinon andrieuxii by (13)CO2 pulse-chase experiments. NMR analyses revealed positional enrichments of (13)C2-isotopologues in both the ... ...

    Abstract The biosynthesis of lupeol-3-(3'R-hydroxy)-stearate (procrim b, 1) was investigated in the Mexican medicinal plant Pentalinon andrieuxii by (13)CO2 pulse-chase experiments. NMR analyses revealed positional enrichments of (13)C2-isotopologues in both the triterpenoid and the hydroxystearate moieties of 1. Five of the six isoprene units reflected a pattern with [1,2-(13)C2]- and [3,5-(13)C2]-isotopologues from the respective C5-precursors, IPP and DMAPP, whereas one isoprene unit in the ring E of 1 showed only the [3,5-(13)C2]-connectivity of the original C5-precursor, due to rearrangement of the dammarenyl cation intermediate during the cyclization process. The presence of (13)C2-isotopologues was indicative of [(13)C2]acetyl-CoA being the precursor units in the formation of the fatty acid moiety and of the triterpene via the mevalonate route. The observed labeling pattern was in agreement with a chair-chair-chair-boat conformation of the (S)-2,3-oxidosqualene precursor during the cyclization process, suggesting that the lupeol synthase from P. andrieuxii is of the same type as that from Olea europea and Taraxacum officinale, but different from that of Arabidopsis thaliana. The study shows that (13)CO2 pulse-chase experiments are powerful in elucidating, under in vivo conditions and in a single experiment, the biosynthesis of complex plant products including higher terpenes.
    MeSH term(s) Amino Acid Sequence ; Carbon Isotopes/chemistry ; Cyclization ; Intramolecular Transferases/chemistry ; Magnetic Resonance Spectroscopy ; Olea/chemistry ; Pentacyclic Triterpenes/biosynthesis ; Pentacyclic Triterpenes/chemical synthesis ; Pentacyclic Triterpenes/chemistry ; Squalene/analogs & derivatives ; Squalene/chemical synthesis ; Squalene/chemistry ; Stearates/chemical synthesis ; Stearates/chemistry ; Taraxacum/chemistry ; Triterpenes/chemical synthesis ; Triterpenes/chemistry
    Chemical Substances 2,3-oxidosqualene ; Carbon Isotopes ; Pentacyclic Triterpenes ; Stearates ; Triterpenes ; lupeol-3-(3'-R-hydroxy)-stearate ; Squalene (7QWM220FJH) ; Intramolecular Transferases (EC 5.4.-) ; lupeol synthase (EC 5.4.99.-) ; lupeol (O268W13H3O)
    Language English
    Publishing date 2014-04-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 123490-0
    ISSN 1520-6904 ; 0022-3263
    ISSN (online) 1520-6904
    ISSN 0022-3263
    DOI 10.1021/jo402677w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Lung ultrasound and postoperative follow-up of congenital diaphragmatic hernia.

    Gregorio-Hernández, R / Ramos-Navarro, C / Vigil-Vázquez, S / Rodríguez-Corrales, E / Pérez-Pérez, A / Arriaga-Redondo, M / Sánchez-Luna, M

    European journal of pediatrics

    2023  Volume 182, Issue 9, Page(s) 3973–3981

    Abstract: Lung ultrasound (LU) has emerged as a valuable tool for assessing pulmonary aeration noninvasively, rapidly, and reliably in different neonatal conditions. However, its role in the preoperative and postoperative evaluation in congenital diaphragmatic ... ...

    Abstract Lung ultrasound (LU) has emerged as a valuable tool for assessing pulmonary aeration noninvasively, rapidly, and reliably in different neonatal conditions. However, its role in the preoperative and postoperative evaluation in congenital diaphragmatic hernia (CDH) is still poorly analyzed. We present a cohort of 8 patients diagnosed with CDH who underwent lung ultrasound examinations at various time points before and after surgical correction. The lung ultrasound patterns were compared between two groups: mechanical ventilation ≤ 7 days (MV ≤ 7) and mechanical ventilation > 7 days (MV > 7). The ultrasound findings were also compared to CT scans and chest X-ray images to assess its diagnostic capacity for identifying postoperative complications: pneumothorax, pleural effusion, and pneumonia. Group MV ≤ 7 exhibited a normal pattern even at 48 h postsurgery, while group MV > 7 presented interstitial or alveolointerstitial pattern in both lungs for prolonged periods (2-3 weeks). Furthermore, contralateral LU pattern may be predictive of respiratory evolution.  Conclusion: Lung ultrasound is a valuable tool for evaluating the progressive reaeration of the lung following surgical correction in CDH patients. It demonstrates the ability to diagnose common postoperative complications without the need for radiation exposure while offering the advantages of quick and serial assessments. These findings highlight the potential of lung ultrasound as an effective alternative to conventional imaging methods in the management of CDH. What is Known: • Lung ultrasound evaluates lung aeration and predicts respiratory outcomes in neonatal patients. What is New: • Lung ultrasound is useful in the postsurgical management of congenital diaphragmatic hernia patients, detecting reaeration and respiratory complications.
    MeSH term(s) Infant, Newborn ; Humans ; Hernias, Diaphragmatic, Congenital/diagnostic imaging ; Hernias, Diaphragmatic, Congenital/surgery ; Follow-Up Studies ; Lung/diagnostic imaging ; Ultrasonography ; Pneumothorax
    Language English
    Publishing date 2023-06-27
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-023-05074-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Point-of-care lung ultrasound in three neonates with COVID-19.

    Gregorio-Hernández, R / Escobar-Izquierdo, A B / Cobas-Pazos, J / Martínez-Gimeno, A

    European journal of pediatrics

    2020  Volume 179, Issue 8, Page(s) 1279–1285

    Abstract: Since March 2020, the world is involved in the COVID-19 pandemic, a disease caused by a novel virus called SARS-CoV-2. Some authors have described the ultrasonographic findings of COVID-19 pneumonia in adults and children, but data on neonates are ... ...

    Abstract Since March 2020, the world is involved in the COVID-19 pandemic, a disease caused by a novel virus called SARS-CoV-2. Some authors have described the ultrasonographic findings of COVID-19 pneumonia in adults and children, but data on neonates are lacking. Our objective was to describe the ultrasonographic lung pattern on newborns with SARS-CoV-2 infection during the COVID-19 pandemic. Newborns who tested positive for SARS-CoV-2 PCR in respiratory samples and were evaluated with point-of-care lung ultrasound (LU) from March to April 2020 were included. LU was performed bedside by a single investigator at the time of diagnosis and every 48 h during the first week following diagnosis. Six areas were studied. Three neonates were included. Infants' comorbidities included meconium aspiration syndrome, bronchopulmonary dysplasia, and Hirschsprung's disease. One required mechanical ventilation. No deaths occurred. LU showed B-lines, consolidation, and spared areas. No pneumothorax or pleural effusion was observedConclusions: LU could be of value when managing COVID-19 neonates. We describe the findings of lung ultrasound monitoring during the first week following diagnosis in three neonates with SARS-CoV-2 infection. What is known: • Lung ultrasound (LU) is a useful tool in COVID-19 management in adults. To date, no report on LU and neonates with SARS-CoV-2 infection has been published. What is new: • This study adds evidence about LU findings in neonates with SARS-CoV-2 infection.
    MeSH term(s) Betacoronavirus ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/complications ; Coronavirus Infections/diagnosis ; Coronavirus Infections/diagnostic imaging ; Humans ; Infant, Newborn ; Lung/diagnostic imaging ; Male ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/diagnostic imaging ; Point-of-Care Systems ; SARS-CoV-2 ; Ultrasonography
    Keywords covid19
    Language English
    Publishing date 2020-06-05
    Publishing country Germany
    Document type Case Reports ; Journal Article ; Video-Audio Media
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-020-03706-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Neonatal pneumothoraces with atypical location: the role of lung ultrasound.

    Gregorio-Hernández, Rebeca / Pérez-Pérez, Alba / Alonso-Ojembarrena, Almudena / Arriaga-Redondo, María / Ramos-Navarro, Cristina / Sánchez-Luna, Manuel

    European journal of pediatrics

    2021  Volume 181, Issue 4, Page(s) 1751–1756

    Abstract: Neonatal pneumothorax (NP) is a potentially life-threatening condition. Lung ultrasound (LUS) has shown higher sensitivity and specificity in diagnosis compared to x-rays, but evidence regarding its usefulness in complex NP is lacking. We report four ... ...

    Abstract Neonatal pneumothorax (NP) is a potentially life-threatening condition. Lung ultrasound (LUS) has shown higher sensitivity and specificity in diagnosis compared to x-rays, but evidence regarding its usefulness in complex NP is lacking. We report four neonates suffering from cardiac or esophageal malformations who developed lateral and/or posterior pneumothoraces, in which LUS helped, making NP diagnosis and management easier and faster.
    Conclusion: LUS is an easy-to-use, fast, simple, and accurate tool when evaluating newborns with NP, also in atypical positions such as in surgical patients.
    What is known: • Lung ultrasound (LUS) has higher sensitivity and specificity than x-rays in the diagnosis of pneumothorax in neonatal patients.
    What is new: • This is the first report about neonatal pneumothorax in non-conventional areas (lateral/posterior) diagnosed by lung ultrasound and how obtaining this information is critical in order to optimize management.
    MeSH term(s) Humans ; Infant, Newborn ; Lung/diagnostic imaging ; Pneumothorax/diagnostic imaging ; Pneumothorax/etiology ; Pneumothorax/therapy ; Radiography ; Sensitivity and Specificity ; Ultrasonography
    Language English
    Publishing date 2021-11-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-021-04329-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Point-of-care lung ultrasound in three neonates with COVID-19

    Gregorio-Hernández, R. / Escobar-Izquierdo, A. B. / Cobas-Pazos, J. / Martínez-Gimeno, A.

    European Journal of Pediatrics

    2020  Volume 179, Issue 8, Page(s) 1279–1285

    Keywords Pediatrics, Perinatology, and Child Health ; covid19
    Language English
    Publisher Springer Science and Business Media LLC
    Publishing country us
    Document type Article ; Online
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-020-03706-4
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: Point-of-care lung ultrasound in three neonates with COVID-19

    Gregorio-Hernández, R / Escobar-Izquierdo, A B / Cobas-Pazos, J / Martínez-Gimeno, A

    Eur J Pediatr

    Abstract: Since March 2020, the world is involved in the COVID-19 pandemic, a disease caused by a novel virus called SARS-CoV-2. Some authors have described the ultrasonographic findings of COVID-19 pneumonia in adults and children, but data on neonates are ... ...

    Abstract Since March 2020, the world is involved in the COVID-19 pandemic, a disease caused by a novel virus called SARS-CoV-2. Some authors have described the ultrasonographic findings of COVID-19 pneumonia in adults and children, but data on neonates are lacking. Our objective was to describe the ultrasonographic lung pattern on newborns with SARS-CoV-2 infection during the COVID-19 pandemic. Newborns who tested positive for SARS-CoV-2 PCR in respiratory samples and were evaluated with point-of-care lung ultrasound (LU) from March to April 2020 were included. LU was performed bedside by a single investigator at the time of diagnosis and every 48 h during the first week following diagnosis. Six areas were studied. Three neonates were included. Infants' comorbidities included meconium aspiration syndrome, bronchopulmonary dysplasia, and Hirschsprung's disease. One required mechanical ventilation. No deaths occurred. LU showed B-lines, consolidation, and spared areas. No pneumothorax or pleural effusion was observedConclusions: LU could be of value when managing COVID-19 neonates. We describe the findings of lung ultrasound monitoring during the first week following diagnosis in three neonates with SARS-CoV-2 infection. What is known: • Lung ultrasound (LU) is a useful tool in COVID-19 management in adults. To date, no report on LU and neonates with SARS-CoV-2 infection has been published. What is new: • This study adds evidence about LU findings in neonates with SARS-CoV-2 infection.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #527042
    Database COVID19

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  10. Article ; Online: Lung ultrasound in preterm infants with respiratory distress: experience in a neonatal intensive care unit.

    Gregorio-Hernández, Rebeca / Arriaga-Redondo, María / Pérez-Pérez, Alba / Ramos-Navarro, Cristina / Sánchez-Luna, Manuel

    European journal of pediatrics

    2019  Volume 179, Issue 1, Page(s) 81–89

    Abstract: Lung ultrasound (LUS) has been described as a useful tool in early prognosis of several respiratory diseases of the newborn, especially preterm infant newborns (PTNB) with respiratory distress syndrome (RDS), but still, it is not a standard of care in ... ...

    Abstract Lung ultrasound (LUS) has been described as a useful tool in early prognosis of several respiratory diseases of the newborn, especially preterm infant newborns (PTNB) with respiratory distress syndrome (RDS), but still, it is not a standard of care in many neonatal units. We have conducted a descriptive, prospective study in a tertiary neonatal unit during 1 year. PTNB less than 35 weeks with respiratory distress at birth on non-invasive ventilation were recruited. A LUS was performed in the first 12 h of life and scored from 6 to 18 points (6 areas, 1 to 3 points each). They were followed until discharge. Main outcomes: need for surfactant treatment. Sixty-four preterm infants, median gestational age 29 weeks. Median LUS score in surfactant group was significantly higher than in no surfactant group (p < 0.0001). LUS ROC curve for surfactant treatment shows AUC 0.97 (IC 95% 0.92-1). LUS Odds ratio for surfactant treatment 3.17 (IC 95% 1.36-7.35).Conclusion: Early high LUS score correlates with surfactant necessity in preterm infants with respiratory distress at birth.What is Known:• Lung ultrasound (LUS) is a useful tool in determining prognosis of preterm infants with respiratory distress at birth.What is New:• This study adds evidence about LUS and preterm infants with respiratory distress, early predicting surfactant need and mechanical ventilation.
    MeSH term(s) Combined Modality Therapy ; Female ; Follow-Up Studies ; Humans ; Infant, Newborn ; Infant, Premature ; Intensive Care Units, Neonatal ; Intensive Care, Neonatal/methods ; Lung/diagnostic imaging ; Male ; Noninvasive Ventilation ; Prognosis ; Prospective Studies ; Pulmonary Surfactants/therapeutic use ; ROC Curve ; Respiratory Distress Syndrome, Newborn/diagnostic imaging ; Respiratory Distress Syndrome, Newborn/therapy ; Ultrasonography
    Chemical Substances Pulmonary Surfactants
    Language English
    Publishing date 2019-10-26
    Publishing country Germany
    Document type Journal Article ; Observational Study
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-019-03470-0
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