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Article ; Online: Clinical and histological wound healing patterns of collagen-based substitutes: An experimental randomized controlled trial in standardized palatal defects in humans.

Bienz, Stefan P / Gadzo, Naida / Zuercher, Anina N / Wiedemeier, Daniel / Jung, Ronald E / Thoma, Daniel S

2023 Volume 51, Issue 3, Page(s) 319–329

Abstract: Aim: To evaluate the progression of wound healing of standardized palatal defects in groups using three different collagen-based wound dressings and a control group, in terms of wound closure, pain perception and descriptive histology.: Materials and ... ...

Abstract	Aim: To evaluate the progression of wound healing of standardized palatal defects in groups using three different collagen-based wound dressings and a control group, in terms of wound closure, pain perception and descriptive histology. Materials and methods: Twenty participants were enrolled in this experimental study, in whom four palatal defects were created. The defects (6 mm diameter, 3 mm depth) were randomly assigned to one of four treatment modalities: C (control), MG (Mucograft®), MD (mucoderm®) and FG (Fibro-Gide®). Photographs were taken, and pain assessment was performed before and after treatment and at 5, 7, 9, 12, 14 and 16 days after surgery. All participants wore a palatal splint for a duration of 16 days. Results: All groups achieved complete wound closure at 14 days. The percentage of the remaining open wound on day 7 amounted to 49.3% (C; interquartile range [IQR]: 22.6), 70.1% (FG; IQR: 20.7), 56.8% (MD; IQR: 26.3) and 62.2% (MG; IQR: 34.4). Statistically significant differences were found between FG and C (p =.01) and between MD and FG (p =.04). None of the participants rated pain higher than 4 out of 10 during the entire study period. Conclusions: Collagen-based wound dressings provide coverage of open defects, albeit without acceleration of wound closure or reduction of pain. FG (which is not intended for open oral wounds) showed slower wound closure compared to C and MD.
MeSH term(s)	Humans ; Wound Healing ; Collagen/therapeutic use ; Bandages ; Palate/surgery ; Pain
Chemical Substances	Collagen (9007-34-5)
Language	English
Publishing date	2023-11-28
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	188647-2
ISSN	1600-051X ; 0303-6979
ISSN (online)	1600-051X
ISSN	0303-6979
DOI	10.1111/jcpe.13903
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Article ; Online: Soft tissue contour changes at implant sites with or without soft tissue grafting in the esthetic zone: A retrospective case-control study with a 12-year follow-up.

Bienz, Stefan P / Carrera, Edwin Ruales / Hüsler, Jürg / Roccuzzo, Andrea / Jung, Ronald E / Thoma, Daniel S

Clinical oral implants research

2023 Volume 34, Issue 5, Page(s) 521–530

Abstract: Objectives: To evaluate the volumetric changes and peri-implant health at implant sites with and without previous soft tissue grafting over a 12-year observation period.: Materials and methods: Eighteen patients received dental implants and ... ...

Abstract	Objectives: To evaluate the volumetric changes and peri-implant health at implant sites with and without previous soft tissue grafting over a 12-year observation period. Materials and methods: Eighteen patients received dental implants and simultaneously guided bone regeneration in the esthetic zone (15-25) for dental rehabilitation. Three months following implant placement, 8 patients (test) received an additional subepithelial connective tissue graft, whereas 10 patients (control) did not receive any additional treatment. One week after prothesis delivery and at the 5 and 12 years follow-up examination, impressions were taken. Obtained casts were processed for profilometric and linear analyses. The mean distance (MD) in the mid-buccal area between the two surfaces was considered the primary outcome. Peri-implant health was assessed based on clinical and radiographic data. Results: Nine female and 7 male patients were re-assessed after a median follow-up time of 144.5 months (Min: 114.8; Max: 213.0). The median reduction of MD amounted to -0.81 mm (Min: -1.39; Max: 0.52) in the test group and -0.56 mm (Min: -0.93; Max: 0.11) in the control group, (intergroup comparison p = .607, CI 95%: -0.760/0.530). None of the implants was diagnosed with peri-implantitis. Six tests and two control implants were diagnosed with peri-implant mucositis (p = .103). Conclusions: Despite the limited number of included patients, similar results in terms of volumetric, linear changes, and peri-implant conditions could be detected at implant sites with or without soft tissue grafting over a period of 12 years.
MeSH term(s)	Humans ; Male ; Female ; Retrospective Studies ; Case-Control Studies ; Follow-Up Studies ; Esthetics, Dental ; Peri-Implantitis ; Treatment Outcome ; Dental Implants ; Connective Tissue/transplantation
Chemical Substances	Dental Implants
Language	English
Publishing date	2023-03-07
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	1067626-0
ISSN	1600-0501 ; 0905-7161
ISSN (online)	1600-0501
ISSN	0905-7161
DOI	10.1111/clr.14058
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Article ; Online: Synthesis of Highly Substituted 3-Pyrrolin-2-ones from N,N-Disubstituted α-Amino Acids.

Samper Barceló, Víctor / Bienz, Stefan

The Journal of organic chemistry

2018 Volume 83, Issue 5, Page(s) 2734–2743

Abstract: Highly functionalized 5-membered N-heterocyclic compounds, 4-aryl-3-chloro-5-methoxy-1-methyl-3-pyrrolin-2-ones, have been synthesized in moderate to high yields by the reaction of N,N-dimethylated aromatic α-amino acids with oxalyl chloride, followed by ...

Abstract	Highly functionalized 5-membered N-heterocyclic compounds, 4-aryl-3-chloro-5-methoxy-1-methyl-3-pyrrolin-2-ones, have been synthesized in moderate to high yields by the reaction of N,N-dimethylated aromatic α-amino acids with oxalyl chloride, followed by solvolysis with MeOH. The products possess a number of functional groups such as an amide, a mixed amido/alkoxy acetal, a vinyl halide, and an alkene and thus are promising candidates to be used as starting materials for the synthesis of diverse five-membered N-heterocyclic compounds.
Language	English
Publishing date	2018--02
Publishing country	United States
Document type	Journal Article
ZDB-ID	123490-0
ISSN	1520-6904 ; 0022-3263
ISSN (online)	1520-6904
ISSN	0022-3263
DOI	10.1021/acs.joc.7b03187
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Article: Travel related histoplasmosis - a diagnostic challenge in a patient with tumor necrosis factor alpha (TNF-α) inhibitor therapy.

Hofmann, Eveline / Mühlethaler, Konrad / Pollak, Matthias / Ott, Daniel / Bienz, Nora / Zimmerli, Stefan / Hirzel, Cédric

Tropical diseases, travel medicine and vaccines

2022 Volume 8, Issue 1, Page(s) 21

Abstract: Introduction: In a non-endemic setting, disseminated histoplasmosis is a rare travel-related health problem of immunosuppressed returnees from endemic regions.: Methods: We describe the case of a 68-year-old man with rheumatoid arthritis and tumor ... ...

Abstract	Introduction: In a non-endemic setting, disseminated histoplasmosis is a rare travel-related health problem of immunosuppressed returnees from endemic regions. Methods: We describe the case of a 68-year-old man with rheumatoid arthritis and tumor necrosis factor alpha (TNF-α) inhibitor treatment-related immunodeficiency, who suffered from disseminated histoplasmosis after traveling to Brazil. Based on this case, we discuss challenges and pitfalls associated with the diagnosis of disseminated histoplasmosis in a non-endemic setting. Results: The disease mimicked a hemophagocytic lymphohistiocytosis (HLH) like syndrome. Histoplasma capsulatum was microscopically detected in bronchoalveolar fluid and bone marrow aspirate smears, but was initially misclassified as Leishmania spp., another class of pathogens, which may cause HLH like syndromes in immunocompromised individuals. Discussion: Since the clinical symptoms of histoplasmosis are nonspecific and physicians in non-endemic regions might not be familiar with this disease pattern, there is a risk of delayed diagnosis of travel related cases. Taking a thorough travel history is key in unclear cases of illness in immunocompromised patients.
Language	English
Publishing date	2022-09-15
Publishing country	England
Document type	Journal Article
ZDB-ID	2835327-4
ISSN	2055-0936
ISSN	2055-0936
DOI	10.1186/s40794-022-00178-2
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Article: A minimally invasive two-stage crown lengthening procedure applying a tunneling technique.

Jung, Ronald E / Bigler, Prisca / Balmer, Marc / Bienz, Stefan P / Hjerppe, Jenni

The international journal of esthetic dentistry

2022 Volume 17, Issue 4, Page(s) 394–406

Abstract: Aim: The purpose of the present retrospective case series was to introduce a minimally invasive two-stage flapless crown lengthening procedure where a gingivectomy was avoided or minimized by reducing the bone height through a tunneling technique.: ... ...

Abstract	Aim: The purpose of the present retrospective case series was to introduce a minimally invasive two-stage flapless crown lengthening procedure where a gingivectomy was avoided or minimized by reducing the bone height through a tunneling technique. Materials and method: Ten patients (median age 46.6 years, range 26.9 to 71.6 years) were included in the study. The indications for performing a crown lengthening procedure were esthetically short clinical crowns (n = 5), asymmetry of the gingival margin level (n = 2), and both esthetically short clinical crowns and asymmetry of the gingival margin level (n = 3). All the patients presented a thick gingival phenotype, and the mean number of operated teeth per patient was 3.7 (range 2 to 8 teeth). Three patients underwent an additional gingivectomy 6 weeks after the crown lengthening procedure. Results: The esthetic and functional outcome of the treatments fulfilled the patients' and dentist's expectations. Conclusion: A minimally invasive two-stage crown lengthening procedure applying a tunneling technique has the potential to achieve predictable esthetic outcomes without an open-flap approach and an additional gingivectomy. However, prospective clinical studies are needed to validate this technique.
MeSH term(s)	Crown Lengthening/methods ; Prospective Studies ; Retrospective Studies ; Esthetics, Dental ; Crowns
Language	English
Publishing date	2022-10-03
Publishing country	Germany
Document type	Journal Article
ISSN	2198-591X
ISSN	2198-591X
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Article ; Online: The influence of thin as compared to thick peri-implant soft tissues on aesthetic outcomes: A systematic review and meta-analysis.

Bienz, Stefan P / Pirc, Miha / Papageorgiou, Spyridon N / Jung, Ronald E / Thoma, Daniel S

Clinical oral implants research

2022 Volume 33 Suppl 23, Page(s) 56–71

Abstract: Objectives: In systematically healthy patients with an implant-supported fixed restoration (P), what is the influence of thin (E) as compared to thick (C) peri-implant soft tissues on aesthetic outcomes (O)?: Methods: Following an a priori protocol, ... ...

Abstract	Objectives: In systematically healthy patients with an implant-supported fixed restoration (P), what is the influence of thin (E) as compared to thick (C) peri-implant soft tissues on aesthetic outcomes (O)? Methods: Following an a priori protocol, a literature search of six databases was conducted up to August 2020 to identify prospective/retrospective clinical studies on healthy patients with an implant-supported fixed reconstruction. Measurement of the buccal soft tissue thickness and an aesthetic outcome was a prerequisite, and sites presenting with a buccal soft tissue thickness of <2 mm or shimmering of a periodontal probe were categorized as a thin phenotype. After study selection, data extraction, and risk of bias assessment, random-effects meta-analysis of Mean Differences (MD) or Odds Ratios (OR) with their corresponding 95% Confidence Intervals (CI) were conducted, followed by sensitivity analyses and assessment of the quality of evidence. Results: Thirty-four unique studies reporting on 1508 patients with 1606 sites were included (9 randomized controlled trials, one controlled trial, 10 prospective cohort studies, 8 cross-sectional studies, and 6 retrospective cohort studies). The mean difference of the pink aesthetic score (PES) after the follow-up was not significantly different between thin (<2.0 mm) or thick soft tissues (≥2.0 mm) or phenotypes (12 studies; MD = 0.15; [95% CI = -0.24, 0.53]; p = .46). PES changes during the follow-up, however, were significantly in favour of thick soft tissues (≥2.0 mm) or phenotypes (p = .05). An increased mean mucosal thickness was associated with an increased papilla index (5 studies; MD = 0.5; [95% CI = 0.1, 0.3]; p = .002) and an increase in papilla presence (5 studies; OR = 1.6; [95% CI = 1.0, 2.3]; p = .03). Thin soft tissues were associated with more recession, -0.62 mm (4 studies; [95% CI = -1.06, -0.18]; p = .006). Patient-reported outcome measures (patient satisfaction) were in favour of thick soft tissues -2.33 (6 studies; [95% CI = -4.70, 0.04]; p = .05). However, the quality of evidence was very low in all instances due to the inclusion of non-randomized studies, high risk of bias and residual confounding. Conclusion: Within the limitations of the present study (weak study designs and various soft tissue measurements or time-points), it can be concluded that increased soft tissue thickness at implant sites was associated with more favourable aesthetic outcomes.
MeSH term(s)	Cross-Sectional Studies ; Dental Implants ; Esthetics ; Humans ; Prospective Studies ; Retrospective Studies
Chemical Substances	Dental Implants
Language	English
Publishing date	2022-06-28
Publishing country	Denmark
Document type	Journal Article ; Meta-Analysis ; Review ; Systematic Review
ZDB-ID	1067626-0
ISSN	1600-0501 ; 0905-7161
ISSN (online)	1600-0501
ISSN	0905-7161
DOI	10.1111/clr.13789
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Article: Early implant placement in sites with ridge preservation or spontaneous healing: histologic, profilometric, and CBCT analyses of an exploratory RCT.

Bienz, Stefan P / Ruales-Carrera, Edwin / Lee, Wan-Zhen / Hämmerle, Christoph H F / Jung, Ronald E / Thoma, Daniel S

Journal of periodontal & implant science

2023

Abstract: Purpose: The aim of this study was to compare changes in soft and hard tissue and the histologic composition following early implant placement in sites with alveolar ridge preservation or spontaneous healing (SH), as well as implant performance up to 1 ... ...

Abstract	Purpose: The aim of this study was to compare changes in soft and hard tissue and the histologic composition following early implant placement in sites with alveolar ridge preservation or spontaneous healing (SH), as well as implant performance up to 1 year after crown insertion. Methods: Thirty-five patients with either intact buccal bone plates or dehiscence of up to 50% following single-tooth extraction of incisors, canines, or premolars were included in the study. They were randomly assigned to undergo one of three procedures: deproteinized bovine bone mineral with 10% collagen (DBBM-C) covered by a collagen matrix (DBBM-C/CM), DBBM-C alone, or SH. At 8 weeks, implant placement was carried out, and cone-beam computed tomography scans and impressions were obtained for profilometric analysis. Patients were followed up after the final crown insertion and again at 1 year post-procedure. Results: Within the first 8 weeks following tooth extraction, the median height of the buccal soft tissue contour changed by -2.11 mm for the DBBM-C/CM group, -1.62 mm for the DBBM-C group, and -1.93 mm for the SH group. The corresponding height of the buccal mineralized tissue changed by -0.27 mm for the DBBM-C/CM group, -2.73 mm for the DBBM-C group, and -1.48mm for the SH group. The median contour changes between crown insertion and 1 year were -0.19 mm in the DBBM-C/CM group, -0.09 mm in the DBBM-C group, and -0.29 mm in the SH group. Conclusions: Major vertical and horizontal ridge contour changes occurred, irrespective of the treatment modality, up to 8 weeks following tooth extraction. The DBBM-C/CM preserved more mineralized tissue throughout this period, despite a substantial reduction in the overall contour. All 3 protocols led to stable tissues for up to 1 year.
Language	English
Publishing date	2023-06-14
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2557339-1
ISSN	2093-2286 ; 2093-2278
ISSN (online)	2093-2286
ISSN	2093-2278
DOI	10.5051/jpis.2300460023
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Article ; Online: Tissue integration and biodegradation of soft tissue substitutes with and without compression: an experimental study in the rat.

Bienz, Stefan P / Vaquette, Cedryck / Ioannidis, Alexis / Hämmerle, Christoph H F / Jung, Ronald E / Ivanovski, Sašo / Thoma, Daniel S

Clinical oral investigations

2022 Volume 27, Issue 1, Page(s) 313–328

Abstract: Objectives: To analyze the influence of compression on tissue integration and degradation of soft tissue substitutes.: Material and methods: Six subcutaneous pouches in twenty-eight rats were prepared and boxes made of Al: Results: The area ... ...

Abstract	Objectives: To analyze the influence of compression on tissue integration and degradation of soft tissue substitutes. Material and methods: Six subcutaneous pouches in twenty-eight rats were prepared and boxes made of Al Results: The area fraction of fibroblasts increased in all groups over time. At 12 weeks, the densely compressed materials FG4 (1.1%), MGA4 (1.7%), and MGA2 (2.5%) obtained lower values as compared to the other groups, ranging between 4.7 (E2) and 6.5% (MG). Statistically significant differences (p ≤ 0.05) were observed between groups FG4 vs MG/FG2/E/E4 as well as between MGA4 vs MG/FG2/E/E4 and E vs MGA2. Conclusions: Higher levels of compression led to delayed tissue integration. The effect of different compression levels was more distinct when compared to the differences between the materials. Clinical relevance: All biomaterials demonstrated tissue integration and a minimal concomitant inflammatory reaction. Clinically, it might be more favorable to obtain a sufficient flap release or to reduce the material size to improve the tissue integration processes.
MeSH term(s)	Rats ; Animals ; Biocompatible Materials/pharmacology ; Collagen ; Skin
Chemical Substances	Biocompatible Materials ; Collagen (9007-34-5)
Language	English
Publishing date	2022-10-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1364490-7
ISSN	1436-3771 ; 1432-6981
ISSN (online)	1436-3771
ISSN	1432-6981
DOI	10.1007/s00784-022-04726-0
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Article ; Online: Chromatographic Separation of the Codonocarpine Type Alkaloids from the Root Bark of

Forster, Yvonne / Ghaffar, Abdul / Bienz, Stefan

Bio-protocol

2017 Volume 7, Issue 4, Page(s) e2144

Abstract: Various parts of the caper ... ...

Abstract	Various parts of the caper tree
Language	English
Publishing date	2017-02-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2833269-6
ISSN	2331-8325 ; 2331-8325
ISSN (online)	2331-8325
ISSN	2331-8325
DOI	10.21769/BioProtoc.2144
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Article ; Online: Soft-tissue dimensional change following guided bone regeneration on peri-implant defects using soft-type block or particulate bone substitutes: 1-year outcomes of a randomized controlled clinical trial.

Song, Young Woo / Bienz, Stefan P / Benic, Goran I / Cha, Jae-Kook / Hämmerle, Christoph H F / Jung, Ui-Won / Jung, Ronald E

Journal of clinical periodontology

2022 Volume 50, Issue 2, Page(s) 147–157

Abstract: Aim: To compare the peri-implant soft-tissue dimensional changes following guided bone regeneration between particulate (particle group) and collagenated soft-block-type (block group) biphasic calcium phosphate (BCP).: Materials and methods: This ... ...

Abstract	Aim: To compare the peri-implant soft-tissue dimensional changes following guided bone regeneration between particulate (particle group) and collagenated soft-block-type (block group) biphasic calcium phosphate (BCP). Materials and methods: This study investigated 35 subjects: 18 in the particle group and 17 in the block group. Cone-beam computed tomography obtained at 6 months post surgery and optical impressions taken periodically (before surgery, 6 months post surgery, and 1 year post surgery) were superimposed. The ridge contour changes over time and the peri-implant mucosal thicknesses were measured diagonally and horizontally, and analysed statistically. Results: The increases in diagonal (1.12 ± 0.78 mm) and horizontal (2.79 ± 1.90 mm) ridge contour of the block group were significantly higher than those in the particle group during the first 6 months (p < .05); however, the contour hardly changed thereafter (diagonal: 0.07 ± 0.75 mm; horizontal: -0.34 ± 1.26 mm), resulting in the 1-year contour changes similar between the two groups. Regardless of the type of BCP, the ridge contour increased significantly over 1 year when the dehiscence defect had a contained configuration (p < .05). Conclusions: The increase in soft-tissue dimensions for 1 year was similar between the two groups. The mucosal contour increase was larger when the surgery was conducted in a more contained defect, and this was not influenced by the type of BCP.
MeSH term(s)	Humans ; Dental Implantation, Endosseous/methods ; Bone Substitutes/therapeutic use ; Guided Tissue Regeneration, Periodontal/methods ; Dental Implants ; Bone Regeneration ; Minerals/therapeutic use ; Alveolar Ridge Augmentation/methods
Chemical Substances	Bone Substitutes ; Dental Implants ; Minerals
Language	English
Publishing date	2022-11-07
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	188647-2
ISSN	1600-051X ; 0303-6979
ISSN (online)	1600-051X
ISSN	0303-6979
DOI	10.1111/jcpe.13738
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