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Article ; Online: Detection of Emerging Pollutants Using Aptamer-Based Biosensors: Recent Advances, Challenges, and Outlook.

Flores-Contreras, Elda A / González-González, Reyna Berenice / González-González, Everardo / Melchor-Martínez, Elda M / Parra-Saldívar, Roberto / Iqbal, Hafiz M N

Biosensors

2022 Volume 12, Issue 12

Abstract: The synergistic potentialities of innovative materials that include aptamers have opened new paradigms in biosensing platforms for high-throughput monitoring systems. The available nucleobase functional moieties in aptamers offer exclusive features for ... ...

Abstract	The synergistic potentialities of innovative materials that include aptamers have opened new paradigms in biosensing platforms for high-throughput monitoring systems. The available nucleobase functional moieties in aptamers offer exclusive features for bioanalytical sensing applications. In this context, compared to various in-practice biological recognition elements, the utilization of aptamers in detection platforms results in an extensive range of advantages in terms of design flexibility, stability, and sensitivity, among other attributes. Thus, the utilization of aptamers-based biosensing platforms is extensively anticipated to meet unaddressed challenges of various in-practice and standard analytical and sensing techniques. Furthermore, the superior characteristics of aptasensors have led to their applicability in the detection of harmful pollutants present in ever-increasing concentrations in different environmental matrices and water bodies, seeking to achieve simple and real-time monitoring. Considering the above-mentioned critiques and notable functional attributes of aptamers, herein, we reviewed aptamers as a fascinating interface to design, develop, and deploy a new generation of monitoring systems to aid modern bioanalytical sensing applications. Moreover, this review aims to summarize the most recent advances in the development and application of aptasensors for the detection of various emerging pollutants (EPs), e.g., pharmaceutical, and personal care products (PPCPs), endocrine-disrupting chemicals (EDCs), pesticides and other agricultural-related compounds, and toxic heavy elements. In addition, the limitations and current challenges are also reviewed, considering the technical constraints and complexity of the environmental samples.
Language	English
Publishing date	2022-11-25
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios12121078
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Modeling COVID-19 epidemics in an Excel spreadsheet to enable first-hand accurate predictions of the pandemic evolution in urban areas.

Alvarez, Mario Moisés / González-González, Everardo / Trujillo-de Santiago, Grissel

Scientific reports

2021 Volume 11, Issue 1, Page(s) 4327

Abstract: COVID-19, the first pandemic of this decade and the second in less than 15 years, has harshly taught us that viral diseases do not recognize boundaries; however, they truly do discriminate between aggressive and mediocre containment responses. We present ...

Abstract	COVID-19, the first pandemic of this decade and the second in less than 15 years, has harshly taught us that viral diseases do not recognize boundaries; however, they truly do discriminate between aggressive and mediocre containment responses. We present a simple epidemiological model that is amenable to implementation in Excel spreadsheets and sufficiently accurate to reproduce observed data on the evolution of the COVID-19 pandemics in different regions [i.e., New York City (NYC), South Korea, Mexico City]. We show that the model can be adapted to closely follow the evolution of COVID-19 in any large city by simply adjusting parameters related to demographic conditions and aggressiveness of the response from a society/government to epidemics. Moreover, we show that this simple epidemiological simulator can be used to assess the efficacy of the response of a government/society to an outbreak. The simplicity and accuracy of this model will greatly contribute to democratizing the availability of knowledge in societies regarding the extent of an epidemic event and the efficacy of a governmental response.
MeSH term(s)	COVID-19/epidemiology ; Disease Outbreaks/statistics & numerical data ; Epidemics/statistics & numerical data ; Humans ; Mexico/epidemiology ; New York City/epidemiology ; Pandemics/statistics & numerical data ; Republic of Korea/epidemiology ; SARS-CoV-2/pathogenicity
Language	English
Publishing date	2021-02-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-83697-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes.

Bravo-González, Sergio / González-González, Everardo / Perales-Salinas, Valeria / Rodríguez-Sánchez, Iram Pablo / Ortiz-Castillo, Jose E / Vargas-Martínez, Adriana / Perez-Gonzalez, Victor H / Luna-Aguirre, Claudia Maribel / Trujillo-de Santiago, Grissel / Alvarez, Mario Moisés

Diagnostics (Basel, Switzerland)

2024 Volume 14, Issue 2

Abstract: COVID-19 made explicit the need for rethinking the way in which we conduct testing for epidemic emergencies. During the COVID-19 pandemic, the dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly ... ...

Abstract	COVID-19 made explicit the need for rethinking the way in which we conduct testing for epidemic emergencies. During the COVID-19 pandemic, the dependence on centralized lab facilities and resource-intensive methodologies (e.g., RT-qPCR methods) greatly limited the deployment of widespread testing efforts in many developed and underdeveloped countries. Here, we illustrate the development of a simple and portable diagnostic kit that enables self-diagnosis of COVID-19 at home from saliva samples. We describe the development of a do-it-yourself (DIY) incubator for Eppendorf tubes that can be used to conduct SARS-CoV-2 detection with competitive sensitivity and selectivity from saliva at home. In a proof-of-concept experiment, we assembled Eppendorf-tube incubators at our home shop, prepared a single-tube mix of reagents and LAMP primers in our lab, and deployed these COVID-19 detection kits using urban delivery systems (i.e., Rappifavor or Uber) to more than 15 different locations in Monterrey, México. This straightforward strategy enabled rapid and cost-effective at-home molecular diagnostics of SARS-CoV-2 from real saliva samples with a high sensitivity (100%) and high selectivity (87%).
Language	English
Publishing date	2024-01-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics14020221
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Article ; Online: Portable and Label-Free Quantitative Loop-Mediated Isothermal Amplification (LF-qLamp) for Reliable COVID-19 Diagnostics in Three Minutes of Reaction Time: Arduino-Based Detection System Assisted by a pH Microelectrode.

Alvarez, Mario Moisés / Bravo-González, Sergio / González-González, Everardo / Trujillo-de Santiago, Grissel

Biosensors

2021 Volume 11, Issue 10

Abstract: Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable ... ...

Abstract	Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 min of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 µL of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.
MeSH term(s)	COVID-19/diagnosis ; COVID-19/virology ; Coronavirus Nucleocapsid Proteins/genetics ; Humans ; Microelectrodes ; Molecular Diagnostic Techniques/instrumentation ; Molecular Diagnostic Techniques/methods ; Nucleic Acid Amplification Techniques/instrumentation ; Nucleic Acid Amplification Techniques/methods ; Phosphoproteins/genetics ; Point-of-Care Systems ; RNA, Viral/analysis ; RNA, Viral/metabolism ; Reaction Time ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Saliva/virology
Chemical Substances	Coronavirus Nucleocapsid Proteins ; Phosphoproteins ; RNA, Viral ; nucleocapsid phosphoprotein, SARS-CoV-2
Language	English
Publishing date	2021-10-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios11100386
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Article ; Online: Emergent Molecular Techniques Applied to the Detection of Porcine Viruses.

Flores-Contreras, Elda A / Carrasco-González, Jorge Alberto / Linhares, Daniel C L / Corzo, Cesar A / Campos-Villalobos, J Israel / Henao-Díaz, Alexandra / Melchor-Martínez, Elda M / Iqbal, Hafiz M N / González-González, Reyna Berenice / Parra-Saldívar, Roberto / González-González, Everardo

Veterinary sciences

2023 Volume 10, Issue 10

Abstract: Molecular diagnostic tests have evolved very rapidly in the field of human health, especially with the arrival of the recent pandemic caused by the SARS-CoV-2 virus. However, the animal sector is constantly neglected, even though accurate detection by ... ...

Abstract	Molecular diagnostic tests have evolved very rapidly in the field of human health, especially with the arrival of the recent pandemic caused by the SARS-CoV-2 virus. However, the animal sector is constantly neglected, even though accurate detection by molecular tools could represent economic advantages by preventing the spread of viruses. In this regard, the swine industry is of great interest. The main viruses that affect the swine industry are described in this review, including African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and porcine circovirus (PCV), which have been effectively detected by different molecular tools in recent times. Here, we describe the rationale of molecular techniques such as multiplex PCR, isothermal methods (LAMP, NASBA, RPA, and PSR) and novel methods such as CRISPR-Cas and microfluidics platforms. Successful molecular diagnostic developments are presented by highlighting their most important findings. Finally, we describe the barriers that hinder the large-scale development of affordable, accessible, rapid, and easy-to-use molecular diagnostic tests. The evolution of diagnostic techniques is critical to prevent the spread of viruses and the development of viral reservoirs in the swine industry that impact the possible development of future pandemics and the world economy.
Language	English
Publishing date	2023-10-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci10100609
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Article ; Online: Modeling COVID-19 epidemics in an Excel spreadsheet to enable first-hand accurate predictions of the pandemic evolution in urban areas

Mario Moisés Alvarez / Everardo González-González / Grissel Trujillo-de Santiago

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 12

Abstract: Abstract COVID-19, the first pandemic of this decade and the second in less than 15 years, has harshly taught us that viral diseases do not recognize boundaries; however, they truly do discriminate between aggressive and mediocre containment responses. ... ...

Abstract	Abstract COVID-19, the first pandemic of this decade and the second in less than 15 years, has harshly taught us that viral diseases do not recognize boundaries; however, they truly do discriminate between aggressive and mediocre containment responses. We present a simple epidemiological model that is amenable to implementation in Excel spreadsheets and sufficiently accurate to reproduce observed data on the evolution of the COVID-19 pandemics in different regions [i.e., New York City (NYC), South Korea, Mexico City]. We show that the model can be adapted to closely follow the evolution of COVID-19 in any large city by simply adjusting parameters related to demographic conditions and aggressiveness of the response from a society/government to epidemics. Moreover, we show that this simple epidemiological simulator can be used to assess the efficacy of the response of a government/society to an outbreak. The simplicity and accuracy of this model will greatly contribute to democratizing the availability of knowledge in societies regarding the extent of an epidemic event and the efficacy of a governmental response.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Microfluidics-Based Biosensing Platforms: Emerging Frontiers in Point-of-Care Testing SARS-CoV-2 and Seroprevalence.

Flores-Contreras, Elda A / González-González, Reyna Berenice / Rodríguez-Sánchez, Iram P / Yee-de León, Juan F / Iqbal, Hafiz M N / González-González, Everardo

Biosensors

2022 Volume 12, Issue 3

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the ongoing COVID-19 (coronavirus disease-2019) outbreak and has unprecedentedly impacted the public health and economic sector. The pandemic has forced researchers to focus on the ... ...

Abstract	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the ongoing COVID-19 (coronavirus disease-2019) outbreak and has unprecedentedly impacted the public health and economic sector. The pandemic has forced researchers to focus on the accurate and early detection of SARS-CoV-2, developing novel diagnostic tests. Among these, microfluidic-based tests stand out for their multiple benefits, such as their portability, low cost, and minimal reagents used. This review discusses the different microfluidic platforms applied in detecting SARS-CoV-2 and seroprevalence, classified into three sections according to the molecules to be detected, i.e., (1) nucleic acid, (2) antigens, and (3) anti-SARS-CoV-2 antibodies. Moreover, commercially available alternatives based on microfluidic platforms are described. Timely and accurate results allow healthcare professionals to perform efficient treatments and make appropriate decisions for infection control; therefore, novel developments that integrate microfluidic technology may provide solutions in the form of massive diagnostics to control the spread of infectious diseases.
MeSH term(s)	Antibodies, Viral/blood ; Biosensing Techniques/methods ; COVID-19/diagnosis ; COVID-19/epidemiology ; Humans ; Microfluidics ; Point-of-Care Systems ; Point-of-Care Testing ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Seroepidemiologic Studies
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2022-03-17
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios12030179
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Characterization of Chemically Activated Pyrolytic Carbon Black Derived from Waste Tires as a Candidate for Nanomaterial Precursor.

González-González, Reyna Berenice / González, Lucy T / Iglesias-González, Sigfrido / González-González, Everardo / Martinez-Chapa, Sergio O / Madou, Marc / Alvarez, Mario Moisés / Mendoza, Alberto

Nanomaterials (Basel, Switzerland)

2020 Volume 10, Issue 11

Abstract: Pyrolysis is a feasible solution for environmental problems related to the inadequate disposal of waste tires, as it leads to the recovery of pyrolytic products such as carbon black, liquid fuels and gases. The characteristics of pyrolytic carbon black ... ...

Abstract	Pyrolysis is a feasible solution for environmental problems related to the inadequate disposal of waste tires, as it leads to the recovery of pyrolytic products such as carbon black, liquid fuels and gases. The characteristics of pyrolytic carbon black can be enhanced through chemical activation in order to produce the required properties for its application. In the search to make the waste tire pyrolysis process profitable, new applications of the pyrolytic solid products have been explored, such as for the fabrication of energy-storage devices and precursor in the synthesis of nanomaterials. In this study, waste tires powder was chemically activated using acid (H
Language	English
Publishing date	2020-11-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano10112213
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Article ; Online: Portable and label-free quantitative Loop-mediated Isothermal Amplification (qLAMP) for reliable COVID-19 diagnostics in 3 minutes: An Arduino-based detection system assisted by a pH microelectrode

Alvarez, Mario Moisés / Bravo-González, Sergio / González-González, Everardo / Trujillo-de Santiago, Grissel

medRxiv

Abstract	Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 minutes of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 μL of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.
Keywords	covid19
Language	English
Publishing date	2021-05-28
Publisher	Cold Spring Harbor Laboratory Press
Document type	Article ; Online
DOI	10.1101/2021.05.23.21256350
Database	COVID19

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Book ; Online: Digital Earth Viewer

Buck, Valentin / Stäbler, Flemming / Gonzalez, Everardo / Greinert, Jens

2023

Abstract: The Digital Earth Viewer is a tool for the visualisation and exploration of geospatial data in true 3D over time. It runs on Windows, MacOS and Linux and only requires a modern webbrowser to use. Common file formats such as CSV, netCDF, GeoTIFF and many ... ...

Abstract	The Digital Earth Viewer is a tool for the visualisation and exploration of geospatial data in true 3D over time. It runs on Windows, MacOS and Linux and only requires a modern webbrowser to use. Common file formats such as CSV, netCDF, GeoTIFF and many more are natively supported
Language	English
Publisher	GEOMAR
Publishing country	de
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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