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Article ; Online: Trabajar con la historia del Holocausto

Debórah Dwork

Revista Mexicana de Ciencias Políticas y Sociales, Vol 61, Iss 228, Pp 235-

2016 Volume 246

Abstract: ... llevado a cabo a partir de entrevistas con v ctimas y sobrevivientes tiene sobre su vida cotidiana. En l ...

Abstract	Este art culo desarrolla una estructura narrativa caleidosc pica para dar cuenta de la historia del Holocausto. En qu medida los historiadores del Holocausto y del genocidio se identi can con las personas sobre quienes escriben? Acaso la historia de aquellas personas se vuelve suya para poder comprenderla? La autora re exiona en torno al estudio de la historia del Holocausto desde una nueva perspectiva: compartiendo desde el sustrato del rigor y el compromiso intelectual la experiencia de los efectos que el trabajo de campo llevado a cabo a partir de entrevistas con v ctimas y sobrevivientes tiene sobre su vida cotidiana. En l se conjugan la especi cad de la tem tica con los nexos siempre complejos entre acontecimiento hist rico, subjetividad y la mirada del investigador.
Keywords	Political science (General) ; JA1-92 ; Political science ; J ; Social sciences (General) ; H1-99 ; Social Sciences ; H
Language	Spanish
Publishing date	2016-01-01T00:00:00Z
Publisher	Universidad Nacional Autónoma de México
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Detection of G Protein-Coupled Receptor Complexes in Postmortem Human Brain by Proximity Ligation Assay.

Zhu, Ying / Dwork, Andrew J / Trifilieff, Pierre / Javitch, Jonathan A

Current protocols in neuroscience

2020 Volume 91, Issue 1, Page(s) e86

Abstract: Combining immunological and molecular biological methods, the antibody-based proximity ligation assay (PLA) has been used for more than a decade to detect and quantify protein-protein interactions, protein modification, and protein expression in situ, ... ...

Abstract	Combining immunological and molecular biological methods, the antibody-based proximity ligation assay (PLA) has been used for more than a decade to detect and quantify protein-protein interactions, protein modification, and protein expression in situ, including in brain tissue. However, the transfer of this technology to human brain samples requires a number of precautions due to the nature of the specimens and their specific processing. Here, we used the PLA brightfield detection technique to assess the expression of dopamine D2 receptor and adenosine A2A receptor and their proximity in human postmortem brains, and we developed a systematic random sampling method to help quantify the PLA signals. © 2019 by John Wiley & Sons, Inc. Basic Protocol 1: Sample preparation and sectioning for PLA_BF Basic Protocol 2: PLA_BF staining of brain tissue Basic Protocol 3: Image acquisition and result analysis Support Protocol: Luxol fast blue/cresyl violet staining.
MeSH term(s)	Animals ; Antigen-Antibody Reactions ; Autopsy/methods ; Brain Chemistry ; Humans ; Immunoassay/methods ; Mice ; Nucleic Acid Amplification Techniques/methods ; Oligonucleotides ; Rabbits ; Receptor, Adenosine A2A/analysis ; Receptors, Dopamine D2/analysis ; Staining and Labeling/methods ; Tissue Fixation/methods
Chemical Substances	ADORA2A protein, human ; Oligonucleotides ; Receptor, Adenosine A2A ; Receptors, Dopamine D2
Language	English
Publishing date	2020-01-14
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2179072-3
ISSN	1934-8576 ; 1934-8584
ISSN (online)	1934-8576
ISSN	1934-8584
DOI	10.1002/cpns.86
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Article: Detecting Borrelia Spirochetes: A Case Study With Validation Among Autopsy Specimens.

Gadila, Shiva Kumar Goud / Rosoklija, Gorazd / Dwork, Andrew J / Fallon, Brian A / Embers, Monica E

Frontiers in neurology

2021 Volume 12, Page(s) 628045

Abstract: The complex etiology of neurodegenerative disease has prompted studies on multiple mechanisms including genetic predisposition, brain biochemistry, immunological responses, and microbial insult. In particular, Lyme disease is often associated with ... ...

Abstract	The complex etiology of neurodegenerative disease has prompted studies on multiple mechanisms including genetic predisposition, brain biochemistry, immunological responses, and microbial insult. In particular, Lyme disease is often associated with neurocognitive impairment with variable manifestations between patients. We sought to develop methods to reliably detect
Language	English
Publishing date	2021-05-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564214-5
ISSN	1664-2295
ISSN	1664-2295
DOI	10.3389/fneur.2021.628045
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Article ; Online: Quantification of the in vivo brain ultrashort-T

Deveshwar, Nikhil / Yao, Jingwen / Han, Misung / Dwork, Nicholas / Shen, Xin / Ljungberg, Emil / Caverzasi, Eduardo / Cao, Peng / Henry, Roland / Green, Ari / Larson, Peder E Z

Magnetic resonance in medicine

2024 Volume 91, Issue 6, Page(s) 2417–2430

Abstract: Purpose: Recent work has shown MRI is able to measure and quantify signals of phospholipid membrane-bound protons associated with myelin in the human brain. This work seeks to develop an improved technique for characterizing this brain ultrashort- : ... ...

Abstract	Purpose: Recent work has shown MRI is able to measure and quantify signals of phospholipid membrane-bound protons associated with myelin in the human brain. This work seeks to develop an improved technique for characterizing this brain ultrashort- Methods: Data from ultrashort echo time scans from 16 healthy volunteers with variable flip angles (VFA) were collected and fitted into an advanced regression model to quantify signal fraction, relaxation time, and frequency shift of the ultrashort- Results: The fitted components show intra-subject differences of different white matter structures and significantly elevated ultrashort- Conclusion: The significantly different measured components and measured
MeSH term(s)	Humans ; Healthy Volunteers ; Protons ; Phantoms, Imaging ; Brain/diagnostic imaging ; Magnetic Resonance Imaging/methods ; Phospholipids
Chemical Substances	Protons ; Phospholipids
Language	English
Publishing date	2024-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	605774-3
ISSN	1522-2594 ; 0740-3194
ISSN (online)	1522-2594
ISSN	0740-3194
DOI	10.1002/mrm.30013
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Article ; Online: Comparative evaluation of two methods for LC-MS/MS proteomic analysis of formalin fixed and paraffin embedded tissues.

Davalieva, Katarina / Kiprijanovska, Sanja / Dimovski, Aleksandar / Rosoklija, Gorazd / Dwork, Andrew J

Journal of proteomics

2021 Volume 235, Page(s) 104117

Abstract: The proteomics of formalin-fixed, paraffin-embedded (FFPE) samples has advanced significantly during the last two decades, but there are many protocols and few studies comparing them directly. There is no consensus on the most effective protocol for ... ...

Abstract	The proteomics of formalin-fixed, paraffin-embedded (FFPE) samples has advanced significantly during the last two decades, but there are many protocols and few studies comparing them directly. There is no consensus on the most effective protocol for shotgun proteomic analysis. We compared the in-solution digestion with RapiGest and Filter Aided Sample Preparation (FASP) of FFPE prostate tissues stored 7 years and mirroring fresh frozen samples, using two label-free data-independent LC-MS/MS acquisitions. RapiGest identified more proteins than FASP, with almost identical numbers of proteins from fresh and FFPE tissues and 69% overlap, good preservation of high-MW proteins, no bias regarding isoelectric point, and greater technical reproducibility. On the other hand, FASP yielded 20% fewer protein identifications in FFPE than in fresh tissue, with 64-69% overlap, depletion of proteins >70 kDa, lower efficiency in acidic and neutral range, and lower technical reproducibility. Both protocols showed highly similar subcellular compartments distribution, highly similar percentages of extracted unique peptides from FFPE and fresh tissues and high positive correlation between the absolute quantitation values of fresh and FFPE proteins. In conclusion, RapiGest extraction of FFPE tissues delivers a proteome that closely resembles the fresh frozen proteome and should be preferred over FASP in biomarker and quantification studies. SIGNIFICANCE: Here we analyzed the performance of two sample preparation methods for shotgun proteomic analysis of FFPE tissues to give a comprehensive overview of the obtained proteomes and the resemblance to its matching fresh frozen counterparts. These findings give us better understanding towards competent proteomics analysis of FFPE tissues. It is hoped that it will encourage further assessments of available protocols before establishing the most effective protocol for shotgun proteomic FFPE tissue analysis.
MeSH term(s)	Chromatography, Liquid ; Formaldehyde ; Humans ; Male ; Paraffin Embedding ; Proteomics ; Reproducibility of Results ; Tandem Mass Spectrometry ; Tissue Fixation
Chemical Substances	Formaldehyde (1HG84L3525)
Language	English
Publishing date	2021-01-14
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2021.104117
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Article ; Online: Brain and blood transcriptome profiles delineate common genetic pathways across suicidal ideation and suicide.

Sun, Shengnan / Liu, Qingkun / Wang, Zhaoyu / Huang, Yung-Yu / Sublette, M Elizabeth / Dwork, Andrew J / Rosoklija, Gorazd / Ge, Yongchao / Galfalvy, Hanga / Mann, J John / Haghighi, Fatemeh

Molecular psychiatry

2024

Abstract: Human genetic studies indicate that suicidal ideation and behavior are both heritable. Most studies have examined associations between aberrant gene expression and suicide behavior, but behavior risk is linked to the severity of suicidal ideation. ... ...

Abstract	Human genetic studies indicate that suicidal ideation and behavior are both heritable. Most studies have examined associations between aberrant gene expression and suicide behavior, but behavior risk is linked to the severity of suicidal ideation. Through a gene network approach, this study investigates how gene co-expression patterns are associated with suicidal ideation and severity using RNA-seq data in peripheral blood from 46 live participants with elevated suicidal ideation and 46 with no ideation. Associations with the presence of suicidal ideation were found within 18 co-expressed modules (p < 0.05), as well as in 3 co-expressed modules associated with suicidal ideation severity (p < 0.05, not explained by severity of depression). Suicidal ideation presence and severity-related gene modules with enrichment of genes involved in defense against microbial infection, inflammation, and adaptive immune response were identified and investigated using RNA-seq data from postmortem brain that revealed gene expression differences with moderate effect sizes in suicide decedents vs. non-suicides in white matter, but not gray matter. Findings support a role of brain and peripheral blood inflammation in suicide risk, showing that suicidal ideation presence and severity are associated with an inflammatory signature detectable in blood and brain, indicating a biological continuity between ideation and suicidal behavior that may underlie a common heritability.
Language	English
Publishing date	2024-01-26
Publishing country	England
Document type	Journal Article
ZDB-ID	1330655-8
ISSN	1476-5578 ; 1359-4184
ISSN (online)	1476-5578
ISSN	1359-4184
DOI	10.1038/s41380-024-02420-z
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Article ; Online: Integrative transcriptome- and DNA methylation analysis of brain tissue from the temporal pole in suicide decedents and their controls.

Sha, Qiong / Fu, Zhen / Escobar Galvis, Martha L / Madaj, Zach / Underwood, Mark D / Steiner, Jennifer A / Dwork, Andrew / Simpson, Norman / Galfalvy, Hanga / Rozoklija, Gorazd / Achtyes, Eric D / Mann, J John / Brundin, Lena

Molecular psychiatry

2023

Abstract: Suicide rates have increased steadily world-wide over the past two decades, constituting a serious public health crisis that creates a significant burden to affected families and the society as a whole. Suicidal behavior involves a multi-factorial ... ...

Abstract	Suicide rates have increased steadily world-wide over the past two decades, constituting a serious public health crisis that creates a significant burden to affected families and the society as a whole. Suicidal behavior involves a multi-factorial etiology, including psychological, social and biological factors. Since the molecular neural mechanisms of suicide remain vastly uncharacterized, we examined transcriptional- and methylation profiles of postmortem brain tissue from subjects who died from suicide as well as their neurotypical healthy controls. We analyzed temporal pole tissue from 61 subjects, largely free from antidepressant and antipsychotic medication, using RNA-sequencing and DNA-methylation profiling using an array that targets over 850,000 CpG sites. Expression of NPAS4, a key regulator of inflammation and neuroprotection, was significantly downregulated in the suicide decedent group. Moreover, we identified a total of 40 differentially methylated regions in the suicide decedent group, mapping to seven genes with inflammatory function. There was a significant association between NPAS4 DNA methylation and NPAS4 expression in the control group that was absent in the suicide decedent group, confirming its dysregulation. NPAS4 expression was significantly associated with the expression of multiple inflammatory factors in the brain tissue. Overall, gene sets and pathways closely linked to inflammation were significantly upregulated, while specific pathways linked to neuronal development were suppressed in the suicide decedent group. Excitotoxicity as well as suppressed oligodendrocyte function were also implicated in the suicide decedents. In summary, we have identified central nervous system inflammatory mechanisms that may be active during suicidal behavior, along with oligodendrocyte dysfunction and altered glutamate neurotransmission. In these processes, NPAS4 might be a master regulator, warranting further studies to validate its role as a potential biomarker or therapeutic target in suicidality.
Language	English
Publishing date	2023-11-08
Publishing country	England
Document type	Journal Article
ZDB-ID	1330655-8
ISSN	1476-5578 ; 1359-4184
ISSN (online)	1476-5578
ISSN	1359-4184
DOI	10.1038/s41380-023-02311-9
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Article ; Online: OB HUB: Remote Electronic Fetal Monitoring Surveillance.

Lowery, Deb / De Leon, Betsy / Krening, Cynthia / Dempsey, Amy / Dwork, Peter / Brou, Lina / Tynes, John / Thompson, Lisa

MCN. The American journal of maternal child nursing

2022 Volume 48, Issue 2, Page(s) 62–68

Abstract: Objective: The purpose of this project was to implement a remote fetal surveillance unit with increased vigilance and timelier responses to electronic fetal monitor tracings to improve neonatal outcomes and increase safety.: Methods: A pilot project, ...

Abstract	Objective: The purpose of this project was to implement a remote fetal surveillance unit with increased vigilance and timelier responses to electronic fetal monitor tracings to improve neonatal outcomes and increase safety. Methods: A pilot project, OB HUB, facilitated implementation of a centralized remote fetal surveillance unit including artificial intelligence software and nurse experts dedicated to fetal monitoring interpretation. A telemetry room was established. Notification parameters were created to promote consistent communication between OB HUB nurses and bedside nurses. Outcomes for term neonates included body cooling, arterial cord pH less than 7.0, Apgar scores less than 7 at 5 minutes, emergency cesarean births, and cesarean births. Surveys were used to evaluate team perceptions of fetal safety. Results: There were 2,407 births 6 months pre OB HUB implementation and 2,582 births during the 6-month trial, for a total sample of 4,989 births included in the analysis. Six births (0.25%) resulted in cooling prior to implementation and 2 (0.08%) cooling events occurred during the trial; these differences were not significant (p = .10). There were no significant differences between groups for neonatal outcomes. Average level of safety perceived by nurses and providers remained relatively unchanged when comparing pre- and postimplementation survey results; however, of those responding, 78.8% of nurses indicated the OB HUB improved safety. Clinical implications: There were few adverse events in either group, thus it was a challenge to demonstrate statistically significant improvement in neonatal outcomes even with a sample of nearly 5,000 births. A larger sample is needed to support clinical utility. The OB HUB was perceived favorably by most of the L&D nurses.
MeSH term(s)	Pregnancy ; Infant, Newborn ; Female ; Humans ; Cardiotocography/methods ; Artificial Intelligence ; Pilot Projects ; Cesarean Section ; Fetal Monitoring
Language	English
Publishing date	2022-12-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	605601-5
ISSN	1539-0683 ; 0361-929X
ISSN (online)	1539-0683
ISSN	0361-929X
DOI	10.1097/NMC.0000000000000891
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Article: Functional Architecture of Brain and Blood Transcriptome Delineate Biological Continuity Between Suicidal Ideation and Suicide.

Sun, Shengnan / Liu, Qingkun / Wang, Zhaoyu / Huang, Yung-Yu / Sublette, M / Dwork, Andrew / Rosoklija, Gorazd / Ge, Yongchao / Galfalvy, Hanga / Mann, J John / Haghighi, Fatemeh

Research square

2023

Abstract	Human genetic studies indicate that suicidal ideation and behavior are both heritable. Most studies have examined associations between aberrant gene expression and suicide behavior, but behavior risk is linked to severity of suicidal ideation. Through a gene network approach, this study investigates how gene co-expression patterns are associated with suicidal ideation and severity using RNA-seq data in peripheral blood from 46 live participants with elevated suicidal ideation and 46 with no ideation. Associations with presence and severity of suicidal ideation were found within 18 and 3 co-expressed modules respectively (p < 0.05), not explained by severity of depression. Suicidal ideation presence and severity-related gene modules with enrichment of genes involved in defense against microbial infection, inflammation, and adaptive immune response were identified, and tested using RNA-seq data from postmortem brain that revealed gene expression differences in suicide decedents vs. non-suicides in white matter, but not gray matter. Findings support a role of brain and peripheral blood inflammation in suicide risk, showing that suicidal ideation presence and severity is associated with an inflammatory signature detectable in blood and brain, indicating a biological continuity between ideation and suicidal behavior that may underlie a common heritability.
Language	English
Publishing date	2023-06-01
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-2958575/v1
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Article: A Human Brain Map of Mitochondrial Respiratory Capacity and Diversity.

bioRxiv : the preprint server for biology

2024

Abstract: Mitochondrial oxidative phosphorylation (OxPhos) powers brain ... ...

Abstract	Mitochondrial oxidative phosphorylation (OxPhos) powers brain activity
Language	English
Publishing date	2024-03-07
Publishing country	United States
Document type	Preprint
DOI	10.1101/2024.03.05.583623
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