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  1. Article: Standardized Measures of Coastal Wetland Condition: Implementation at a Laurentian Great Lakes Basin-Wide Scale

    Uzarski, Donald G / Valerie J. Brady / Matthew J. Cooper / Douglas A. Wilcox / Dennis A. Albert / Richard P. Axler / Peg Bostwick / Terry N. Brown / Jan J. H. Ciborowski / Nicholas P. Danz / Joseph P. Gathman / Thomas M. Gehring / Greg P. Grabas / Anne Garwood / Robert W. Howe / Lucinda B. Johnson / Gary A. Lamberti / Ashley H. Moerke / Brent A. Murry /
    Gerald J. Niemi / Christopher J. Norment / Carl R. Ruetz III / Alan D. Steinman / Douglas C. Tozer / Ryan Wheeler / T. Kevin O’Donnell / John P. Schneider

    Wetlands. 2017 Feb., v. 37, no. 1

    2017  

    Abstract: Since European settlement, over 50 % of coastal wetlands have been lost in the Laurentian Great Lakes basin, causing growing concern and increased monitoring by government agencies. For over a decade, monitoring efforts have focused on the development ... ...

    Abstract Since European settlement, over 50 % of coastal wetlands have been lost in the Laurentian Great Lakes basin, causing growing concern and increased monitoring by government agencies. For over a decade, monitoring efforts have focused on the development of regional and organism-specific measures. To facilitate collaboration and information sharing between public, private, and government agencies throughout the Great Lakes basin, we developed standardized methods and indicators used for assessing wetland condition. Using an ecosystem approach and a stratified random site selection process, birds, anurans, fish, macroinvertebrates, vegetation, and physico-chemical conditions were sampled in coastal wetlands of all five Great Lakes including sites from the United States and Canada. Our primary objective was to implement a standardized basin-wide coastal wetland monitoring program that would be a powerful tool to inform decision-makers on coastal wetland conservation and restoration priorities throughout the Great Lakes basin.
    Keywords Anura ; birds ; ecosystems ; fish ; government agencies ; macroinvertebrates ; monitoring ; physicochemical properties ; vegetation ; wetland conservation ; wetlands ; Canada ; Great Lakes ; United States
    Language English
    Dates of publication 2017-02
    Size p. 15-32.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 1097341-2
    ISSN 1943-6246 ; 0277-5212
    ISSN (online) 1943-6246
    ISSN 0277-5212
    DOI 10.1007/s13157-016-0835-7
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Exploiting Highly Ordered Subnanoliter Volume Microcapillaries as Microtools for the Analysis of Antibody Producing Cells

    Fitzgerald, Valerie / Leonard Paul / Manning Brian / O’Donnell Barry / O’Kennedy Richard / O’Reilly Brian / O’Sullivan Dermot

    Analytical chemistry. 2015 Jan. 20, v. 87, no. 2

    2015  

    Abstract: ... of this platform in identifying target specific antibodies from bacterial, hybridoma, and B cell libraries. ...

    Abstract The interrogation of highly diverse repertoires of heterogeneous cell populations on a single cell basis increases the likelihood that a cell with unique characteristics will be identified. We have developed a new single cell analysis system comprising millions of bundled subnanoliter volume bioincubation chambers for the identification and recovery of target specific antibody secreting cells (ASCs). This platform integrates dual surface screening with dedicated user driven data analysis and automated cell recovery enabling multiple biophysical parameters to be tracked for millions of antibody leads in parallel. This direct clone analysis and selection technology is a clear deviation from current microfabricated well-based approaches and offers drastically enhanced screening throughput, simultaneous dual surface analysis, and rapid automated single cell recovery. The technology is also applicable to screening both bacterial and mammalian antibody secreting cells. We demonstrate the implementation and feasibility of this platform in identifying target specific antibodies from bacterial, hybridoma, and B cell libraries.
    Keywords antibodies ; hybridomas ; mammals ; screening
    Language English
    Dates of publication 2015-0120
    Size p. 997-1003.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021%2Fac503547j
    Database NAL-Catalogue (AGRICOLA)

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