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Article ; Online: Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration.

Hata, Akihiko / Meuchi, Yuno / Liu, Miaomiao / Torii, Shotaro / Katayama, Hiroyuki

2023 Volume 15, Issue 1, Page(s) 8–20

Abstract: The hollow fiber ultrafiltration (HFUF)-based microbial concentration method is widely applied for monitoring pathogenic viruses and microbial indicators in environmental water samples. However, the HFUF-based method can co-concentrate substances that ... ...

Abstract	The hollow fiber ultrafiltration (HFUF)-based microbial concentration method is widely applied for monitoring pathogenic viruses and microbial indicators in environmental water samples. However, the HFUF-based method can co-concentrate substances that interfere with downstream molecular processes-nucleic acid extraction, reverse transcription (RT), and PCR. These inhibitory substances are assumed to be hydrophobic and, therefore, expected to be excluded by a simple surfactant treatment before the silica membrane-based RNA extraction process. In this study, the efficacy and limitations of the sodium deoxycholate (SD) treatment were assessed by quantifying a process control and indigenous viruses using 42 surface water samples concentrated with HFUF. With some exceptions, which tended to be seen in samples with high turbidity (> 4.0 NTU), virus recovery by the ultrafiltration method was sufficiently high (> 10%). RNA extraction-RT-quantitative PCR (RT-qPCR) efficiency of the process control was insufficient (10%) for 30 of the 42 HFUF concentrates without any pretreatments, but it was markedly improved for 21 of the 30 inhibitory concentrates by the SD treatment. Detection rates of indigenous viruses were also improved and no substantial loss of viral RNA was observed. The SD treatment was particularly effective in mitigating RT-qPCR inhibition, although it was not effective in improving RNA extraction efficiency. The methodology is simple and easily applied. These findings indicate that SD treatment can be a good alternative to sample dilution, which is widely applied to mitigate the effect of RT-qPCR inhibition, and can be compatible with other countermeasures.
MeSH term(s)	Ultrafiltration/methods ; Water ; Surface-Active Agents ; Enterovirus ; Viruses ; RNA ; Water Microbiology
Chemical Substances	Water (059QF0KO0R) ; Surface-Active Agents ; RNA (63231-63-0)
Language	English
Publishing date	2023-01-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2487173-4
ISSN	1867-0342 ; 1867-0334
ISSN (online)	1867-0342
ISSN	1867-0334
DOI	10.1007/s12560-022-09543-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Activity- and gene-based quantification of enteric viruses, F- specific RNA phage genogroups, pepper mild mottle virus, and Escherichia coli in surface water.

Hata, Akihiko / Meuchi, Yuno / Liu, Miaomiao / Torii, Shotaro / Katayama, Hiroyuki

The Science of the total environment

2023 Volume 904, Page(s) 166338

Abstract: Polymerase chain reaction (PCR) is widely applied for the monitoring of pathogenic viruses in water environments. To date, several pretreatments to selectively detect genes from infectious viruses via PCR have been developed. This study was aimed to ... ...

Abstract	Polymerase chain reaction (PCR) is widely applied for the monitoring of pathogenic viruses in water environments. To date, several pretreatments to selectively detect genes from infectious viruses via PCR have been developed. This study was aimed to characterize and validate methods for quantifying active viruses and indicators and to evaluate the proportion of their active fractions in surface water (n = 42). Active E. coli and F-specific RNA phage (FRNAPH) genogroups were quantified using culture assays. In addition to these microbes, norovirus genogroups I (GI) and II, Aichi virus 1, and pepper mild mottle virus (PMMoV) were quantified by (reverse transcription)-quantitative PCR (RT-qPCR) with and without cis-dichlorodiammineplatinum (CDDP) treatment to exclude genes in inactive viruses. CDDP-RT-qPCR showed concentrations and detection frequencies comparable to or higher than culture assays. Consequently, although CDDP-RT-qPCR can suggest the presence of an inactive virus, it can also overestimate the activity of the virus in the environment. Differences between culture and CDDP-RT-qPCR and between CDDP-RT-qPCR and RT-qPCR varied among the viruses. CDDP-RT-qPCR showed a concentration comparable to the culture assay (within 1 log
MeSH term(s)	Water ; Escherichia coli ; RNA Phages/genetics ; Enterovirus ; Viruses ; Genotype
Chemical Substances	Water (059QF0KO0R)
Language	English
Publishing date	2023-08-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2023.166338
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Article ; Online: Potential Sensitivity of Wastewater Monitoring for SARS-CoV-2: Comparison with Norovirus Cases.

Hata, Akihiko / Honda, Ryo

Environmental science & technology

2020 Volume 54, Issue 11, Page(s) 6451–6452

MeSH term(s)	Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Humans ; Norovirus ; Pandemics ; Pneumonia, Viral ; Severe acute respiratory syndrome-related coronavirus ; SARS-CoV-2 ; Wastewater
Chemical Substances	Waste Water
Keywords	covid19
Language	English
Publishing date	2020-05-18
Publishing country	United States
Document type	News
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.0c02271
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Article ; Online: Applicability of F-specific bacteriophage subgroups, PMMoV and crAssphage as indicators of source specific fecal contamination and viral inactivation in rivers in Japan.

Meuchi, Yuno / Nakada, Miu / Kuroda, Keisuke / Hanamoto, Seiya / Hata, Akihiko

PloS one

2023 Volume 18, Issue 7, Page(s) e0288454

Abstract: To date, several microbes have been proposed as potential source-specific indicators of fecal pollution. 16S ribosomal RNA gene markers of the Bacteroidales species are the most widely applied due to their predominance in the water environment and source ...

Abstract	To date, several microbes have been proposed as potential source-specific indicators of fecal pollution. 16S ribosomal RNA gene markers of the Bacteroidales species are the most widely applied due to their predominance in the water environment and source specificity. F-specific bacteriophage (FPH) subgroups, especially FRNA phage genogroups, are also known as potential source-specific viral indicators. Since they can be quantified by both culture-based and molecular assays, they may also be useful as indicators for estimating viral inactivation in the environment. Pepper mild mottle virus (PMMoV) and crAssphage, which are frequently present in human feces, are also potentially useful as human-specific indicators of viral pollution. This study aimed to evaluate the applicability of FPH subgroups, PMMoV, and crAssphage as indicators of source-specific fecal contamination and viral inactivation using 108 surface water samples collected at five sites affected by municipal and pig farm wastewater. The host specificity of the FPH subgroups, PMMoV, and crAssphage was evaluated by principal component analysis (PCA) along with other microbial indicators, such as 16S ribosomal RNA gene markers of the Bacteroidales species. The viabilities (infectivity indices) of FRNA phage genogroups were estimated by comparing their numbers determined by infectivity-based and molecular assays. The PCA explained 58.2% of the total information and classified microbes into three groups: those considered to be associated with pig and human fecal contamination and others. Infective and gene of genogroup IV (GIV)-FRNA phage were assumed to be specific to pig fecal contamination, while the genes of GII-FRNA phage and crAssphage were identified to be specific to human fecal contamination. However, PMMoV, infective GI-FRNA phage, and FDNA phage were suggested to not be specific to human or pig fecal contamination. FRNA phage genogroups, especially the GIV-FRNA phage, were highly inactivated in the warm months in Japan (i.e., July to November). Comparing the infectivity index of several FRNA phage genogroups or other viruses may provide further insight into viral inactivation in the natural environment and by water treatments.
MeSH term(s)	Humans ; Animals ; Swine ; Bacteriophages/genetics ; Rivers ; Japan ; Virus Inactivation ; Water Pollution/analysis ; Feces ; Water Microbiology
Language	English
Publishing date	2023-07-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0288454
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Article ; Online: Dose-response Associations of Physical Activity and Sitting Time With All-cause Mortality in Older Japanese Adults

Satoshi Seino / Takumi Abe / Yu Nofuji / Toshiki Hata / Shoji Shinkai / Akihiko Kitamura / Yoshinori Fujiwara

Journal of Epidemiology, Vol 34, Iss 1, Pp 23-

2024 Volume 30

Abstract: Purpose: Although examining the dose-response curves of physical activity (PA) and sitting time with health-related outcomes is an important research agenda, the results for older Japanese adults are extremely limited. We examined the dose-response ... ...

Abstract	Purpose: Although examining the dose-response curves of physical activity (PA) and sitting time with health-related outcomes is an important research agenda, the results for older Japanese adults are extremely limited. We examined the dose-response associations of PA and sitting time with all-cause mortality among older Japanese. Methods: Initially, 8,069 non-disabled residents (4,073 men; 3,996 women) aged 65–84 years of Ota City, Japan, were analyzed. Moderate-to-vigorous PA (MVPA) and sitting time were evaluated using the International Physical Activity Questionnaire-Short Form. Multivariate-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) of MVPA and sitting time for all-cause mortality were calculated, and the dose-response curves were examined using restricted cubic splines (RCS). Results: During 4.1 years of follow-up, 458 participants (5.7%; 331 men and 127 women) died. Compared with the low MVPA (<600 metabolic equivalents [METs]·minutes/week) group, HR for mortality gradually reduced in moderate (600–3,000 METs·minutes/week) and high (>3,000 METs·minutes/week) MVPA groups (moderate: HR 0.66; 95% CI, 0.54–0.82; high: HR 0.58; 95% CI, 0.45–0.75; P < 0.001 for trend). RCS showed that the HR for mortality reduced linearly up to approximately 2,000 METs·minutes/week of MVPA, and maximal risk reduction was seen at approximately 3,000–4,500 METs·minutes/week of MVPA. No significant dose-response association of sitting time with mortality was observed. Conclusion: Higher MVPA levels reduced all-cause mortality risk, in a significant inverse non-linear dose-response manner. Sitting time was not significantly associated with all-cause mortality. It is important to disseminate the significance of even a slight increase in the MVPA for reducing mortality risk.
Keywords	physical activity ; sitting time ; mortality ; dose-response ; ipaq-short ; guidelines ; Medicine (General) ; R5-920
Subject code	310
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Japan Epidemiological Association
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration

Hata, Akihiko / Meuchi, Yuno / Liu, Miaomiao / Torii, Shotaro / Katayama, Hiroyuki

Food Environ Virol. 2023 Mar., v. 15, no. 1 p.8-20

2023

Abstract	The hollow fiber ultrafiltration (HFUF)-based microbial concentration method is widely applied for monitoring pathogenic viruses and microbial indicators in environmental water samples. However, the HFUF-based method can co-concentrate substances that interfere with downstream molecular processes—nucleic acid extraction, reverse transcription (RT), and PCR. These inhibitory substances are assumed to be hydrophobic and, therefore, expected to be excluded by a simple surfactant treatment before the silica membrane-based RNA extraction process. In this study, the efficacy and limitations of the sodium deoxycholate (SD) treatment were assessed by quantifying a process control and indigenous viruses using 42 surface water samples concentrated with HFUF. With some exceptions, which tended to be seen in samples with high turbidity (> 4.0 NTU), virus recovery by the ultrafiltration method was sufficiently high (> 10%). RNA extraction-RT-quantitative PCR (RT-qPCR) efficiency of the process control was insufficient (10%) for 30 of the 42 HFUF concentrates without any pretreatments, but it was markedly improved for 21 of the 30 inhibitory concentrates by the SD treatment. Detection rates of indigenous viruses were also improved and no substantial loss of viral RNA was observed. The SD treatment was particularly effective in mitigating RT-qPCR inhibition, although it was not effective in improving RNA extraction efficiency. The methodology is simple and easily applied. These findings indicate that SD treatment can be a good alternative to sample dilution, which is widely applied to mitigate the effect of RT-qPCR inhibition, and can be compatible with other countermeasures.
Keywords	RNA ; deoxycholic acid ; genes ; hydrophobicity ; process control ; reverse transcription ; silica ; surface water ; surfactants ; turbidity ; ultrafiltration ; viruses
Language	English
Dates of publication	2023-03
Size	p. 8-20.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	2487173-4
ISSN	1867-0342 ; 1867-0334
ISSN (online)	1867-0342
ISSN	1867-0334
DOI	10.1007/s12560-022-09543-y
Database	NAL-Catalogue (AGRICOLA)

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Article: Protective effects of dog ownership against the onset of disabling dementia in older community-dwelling Japanese: A longitudinal study.

Taniguchi, Yu / Seino, Satoshi / Ikeuchi, Tomoko / Hata, Toshiki / Shinkai, Shoji / Kitamura, Akihiko / Fujiwara, Yoshinori

Preventive medicine reports

2023 Volume 36, Page(s) 102465

Abstract: This prospective study examined the associations of dog/cat ownership with incident disabling dementia using propensity score matching based on the physical, social, and psychological characteristics of dog and cat owners. We also examined associations ... ...

Abstract	This prospective study examined the associations of dog/cat ownership with incident disabling dementia using propensity score matching based on the physical, social, and psychological characteristics of dog and cat owners. We also examined associations of the interaction between dog/cat ownership and exercise habit and social isolation with dementia. Overall, 11,194 older adults selected using stratified and random sampling strategies in 2016 were analyzed. Dog/cat ownership was defined as "current" or "past and never". Disabling dementia was defined according to physicians' rating in the long-term care insurance system in Japan during the approximately 4-year follow-up period. Statistical analysis was weighted by the inverse of the propensity score in the generalized estimating equation after adjusting for follow-up period. Current dog owners (8.6 %) had an odds ratio (OR) of 0.60 (95 %CI: 0.37-0.977) of having disabling dementia compared to past and never owners. For cat ownership, the corresponding OR was 0.98 (95 %CI: 0.62-1.55). Current dog owners with a regular exercise habit had an OR of 0.37 (0.20-0.68) compared to past and never dog owners with no exercise habit. Further, current dog owners with no social isolation had an OR of 0.41 (0.23-0.73) compared to past and never dog owners with social isolation. Dog ownership had a suppressive effect on incident disabling dementia after adjusting for background factors over a 4-year follow-up period. Specifically, dog owners with an exercise habit and no social isolation had a significantly lower risk of disabling dementia.
Language	English
Publishing date	2023-10-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2785569-7
ISSN	2211-3355
ISSN	2211-3355
DOI	10.1016/j.pmedr.2023.102465
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Article: Potential Sensitivity of Wastewater Monitoring for SARS-CoV-2: Comparison with Norovirus Cases

Hata, Akihiko / Honda, Ryo

Environ Sci Technol

Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #291493
Database	COVID19

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Article ; Online: Dose-response Associations of Physical Activity and Sitting Time With All-cause Mortality in Older Japanese Adults.

Seino, Satoshi / Abe, Takumi / Nofuji, Yu / Hata, Toshiki / Shinkai, Shoji / Kitamura, Akihiko / Fujiwara, Yoshinori

Journal of epidemiology

2023 Volume 34, Issue 1, Page(s) 23–30

Abstract	Purpose: Although examining the dose-response curves of physical activity (PA) and sitting time with health-related outcomes is an important research agenda, the results for older Japanese adults are extremely limited. We examined the dose-response associations of PA and sitting time with all-cause mortality among older Japanese. Methods: Initially, 8,069 non-disabled residents (4,073 men; 3,996 women) aged 65-84 years of Ota City, Japan, were analyzed. Moderate-to-vigorous PA (MVPA) and sitting time were evaluated using the International Physical Activity Questionnaire-Short Form. Multivariate-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) of MVPA and sitting time for all-cause mortality were calculated, and the dose-response curves were examined using restricted cubic splines (RCS). Results: During 4.1 years of follow-up, 458 participants (5.7%; 331 men and 127 women) died. Compared with the low MVPA (<600 metabolic equivalents [METs]·minutes/week) group, HR for mortality gradually reduced in moderate (600-3,000 METs·minutes/week) and high (>3,000 METs·minutes/week) MVPA groups (moderate: HR 0.66; 95% CI, 0.54-0.82; high: HR 0.58; 95% CI, 0.45-0.75; P < 0.001 for trend). RCS showed that the HR for mortality reduced linearly up to approximately 2,000 METs·minutes/week of MVPA, and maximal risk reduction was seen at approximately 3,000-4,500 METs·minutes/week of MVPA. No significant dose-response association of sitting time with mortality was observed. Conclusion: Higher MVPA levels reduced all-cause mortality risk, in a significant inverse non-linear dose-response manner. Sitting time was not significantly associated with all-cause mortality. It is important to disseminate the significance of even a slight increase in the MVPA for reducing mortality risk.
MeSH term(s)	Male ; Adult ; Humans ; Female ; Aged ; Japan/epidemiology ; Exercise/physiology ; Proportional Hazards Models ; Data Collection ; Sedentary Behavior
Language	English
Publishing date	2023-04-28
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1442118-5
ISSN	1349-9092 ; 0917-5040
ISSN (online)	1349-9092
ISSN	0917-5040
DOI	10.2188/jea.JE20220246
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Article ; Online: Efficacy and safety of sheathless guiding catheter for distal radial approach.

Yamada, Takeshi / Izumikawa, Takuya / Kawakami, Shiori / Taniguchi, Norimasa / Hata, Tetsuya / Nakajima, Shunsuke / Takahashi, Akihiko

Cardiovascular revascularization medicine : including molecular interventions

2023 Volume 61, Page(s) 64–67

Abstract: Objectives: This study aimed to assess the efficacy and safety of a sheathless guiding system in patients who underwent percutaneous coronary intervention (PCI) with distal radial access (DRA).: Background: Hyperion™ Sheathless® guiding catheter ( ... ...

Abstract	Objectives: This study aimed to assess the efficacy and safety of a sheathless guiding system in patients who underwent percutaneous coronary intervention (PCI) with distal radial access (DRA). Background: Hyperion™ Sheathless® guiding catheter (Asahi Intecc, Japan), one of the latest 6-Fr guiding systems for transradial PCI characterized by novel outer dilator, is considered to facilitate smooth insertion of the catheter to the artery and may contribute to further reduction of access site complications in DRA. Methods: Between October 2018 and January 2023, 286 patients underwent PCI with a Hyperion™ Sheathless® guiding catheter with DRA at two Japanese hospitals. Procedural success, bleeding complications, and radial artery occlusion (RAO) detected by Doppler ultrasonography were assessed. Results: Mean age of the patients was 72.7 years, and 236 patients (82.5 %) were male. The target lesions were located in the right coronary artery, left anterior descending artery, left circumflex artery, and left main trunk in 81, 44, 50 patients, and 18 patients respectively. Procedural success rate was 99.7 % with no patients requiring conversion to conventional radial access. Two patients presented with a forearm hematoma equivalent to an Early Discharge After Transradial Stenting of Coronary Arteries Study hematoma classification Grade II and 23 with Grade I. No patient presented minor or major bleeding according to the Thrombolysis in Myocardial Infarction bleeding classification. RAO at 30-day follow-up was observed in 6 out of 277 patients (2.2 %). Conclusions: 6-Fr sheathless guiding system for PCI via DRA is feasible and associated with a low incidence of access site complications.
MeSH term(s)	Humans ; Male ; Aged ; Female ; Percutaneous Coronary Intervention/adverse effects ; Cardiac Catheters ; Treatment Outcome ; Radial Artery/diagnostic imaging ; Catheters ; Hematoma/etiology ; Coronary Angiography
Language	English
Publishing date	2023-11-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	2212113-4
ISSN	1878-0938 ; 1553-8389
ISSN (online)	1878-0938
ISSN	1553-8389
DOI	10.1016/j.carrev.2023.11.011
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