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  1. Article ; Online: Iterative Reconstruction of Micro Computed Tomography Scans Using Multiple Heterogeneous GPUs.

    Chou, Wen-Hsiang / Wu, Cheng-Han / Jin, Shih-Chun / Chen, Jyh-Cheng

    Sensors (Basel, Switzerland)

    2024  Volume 24, Issue 6

    Abstract: Graphics processing units (GPUs) facilitate massive parallelism and high-capacity storage, and thus are suitable for the iterative reconstruction of ultrahigh-resolution micro computed tomography (CT) scans by on-the-fly system matrix (OTFSM) calculation ...

    Abstract Graphics processing units (GPUs) facilitate massive parallelism and high-capacity storage, and thus are suitable for the iterative reconstruction of ultrahigh-resolution micro computed tomography (CT) scans by on-the-fly system matrix (OTFSM) calculation using ordered subsets expectation maximization (OSEM). We propose a finite state automaton (FSA) method that facilitates iterative reconstruction using a heterogeneous multi-GPU platform through parallelizing the matrix calculations derived from a ray tracing system of ordered subsets. The FSAs perform flow control for parallel threading of the heterogeneous GPUs, which minimizes the latency of launching ordered-subsets tasks, reduces the data transfer between the main system memory and local GPU memory, and solves the memory-bound of a single GPU. In the experiments, we compared the operation efficiency of OS-MLTR for three reconstruction environments. The heterogeneous multiple GPUs with job queues for high throughput calculation speed is up to five times faster than the single GPU environment, and that speed up is nine times faster than the heterogeneous multiple GPUs with the FIFO queues of the device scheduling control. Eventually, we proposed an event-triggered FSA method for iterative reconstruction using multiple heterogeneous GPUs that solves the memory-bound issue of a single GPU at ultrahigh resolutions, and the routines of the proposed method were successfully executed on each GPU simultaneously.
    Language English
    Publishing date 2024-03-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s24061947
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Longitudinal outcomes of trismus release with free-flap reconstruction in primary and secondary oral submucous fibrosis-related trismus.

    Chen, Jyh-Kwei / Kao, Wen-Hsiang / Huang, Pin-Hsuan / Tsao, Chung-Kan / Nee, Tzer-En

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS

    2024  Volume 92, Page(s) 26–32

    Abstract: Background: Oral submucous fibrosis is a global health concern associated with betel quid use and results in trismus, which can be either primary or secondary in origin. Severe cases often require trismus release with free-flap reconstruction. This ... ...

    Abstract Background: Oral submucous fibrosis is a global health concern associated with betel quid use and results in trismus, which can be either primary or secondary in origin. Severe cases often require trismus release with free-flap reconstruction. This study examined longitudinal outcome trends following trismus release and compared the outcomes of patients with primary and secondary oral submucous fibrosis-related trismus.
    Methods: We conducted a retrospective cohort study by including patients who underwent trismus release between 2013 and 2022. All procedures were performed by a single surgical team to ensure technique standardisation. We measured the maximum mouth opening, the interincisal distance, perioperatively and 1, 2, 3, 4, 6 and 12 months post-operatively. Data were analysed using generalised estimating equations.
    Results: A total of 35 patients were included in the study, 17 with primary and 18 with secondary oral submucous fibrosis-related trismus. Initially, patients with primary oral submucous fibrosis-related trismus had greater interincisal distance gains than those with secondary oral submucous fibrosis-related trismus (p = 0.015 and p = 0.025 at 3 and 4 months post-operatively, respectively). However, after 12 months, this initial advantage faded, with comparable interincisal distance improvements in patients with primary and secondary disease, despite the more complex surgical procedures required in secondary cases.
    Conclusion: Surgeons should carefully consider the benefits of trismus release procedures for patients with secondary oral submucous fibrosis-related trismus by recognising the changes in post-operative outcomes.
    Language English
    Publishing date 2024-02-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2217750-4
    ISSN 1878-0539 ; 1748-6815 ; 0007-1226
    ISSN (online) 1878-0539
    ISSN 1748-6815 ; 0007-1226
    DOI 10.1016/j.bjps.2024.02.040
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book: Chen, Hsiang-yen chiao-shou lun-wen-chi

    Chen, H

    1979  

    Institution Nan-chin Ta-hsu͏̈eh Hsu͏̈eh-pao Pien-chi-pu
    Author's details H. Chen. Nan-chin Ta-hsu͏̈eh Hsu͏̈eh-pao Pien-chi-pu
    Size 112 S
    Publishing place Nanking
    Document type Book
    Note Collected papers of Professor Chen, Hsiang-yen
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  4. Article ; Online: Effects of early aquatic exercise intervention on trunk strength and functional recovery of patients with lumbar fusion: a randomized controlled trial.

    Huang, An-Hua / Chou, Wen-Hsiang / Wang, Wendy Tzyy-Jiuan / Chen, Wen-Yin / Shih, Yi-Fen

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 10716

    Abstract: This study investigated the effectiveness of an early aquatic exercise program on trunk muscle function and functional recovery of patients with lumbar fusion. Twenty-eight subjects were divided into two equal groups. Patients in the aquatic group ... ...

    Abstract This study investigated the effectiveness of an early aquatic exercise program on trunk muscle function and functional recovery of patients with lumbar fusion. Twenty-eight subjects were divided into two equal groups. Patients in the aquatic group performed two 60-min aquatic exercise sessions and three 60-min home exercise sessions per week for 6 weeks, whereas those in the control group performed five sessions of 60-min home exercises per week for 6 weeks. The primary outcomes were the Numerical Pain Rating Scale (NPRS) and Oswestry Disability Index (ODI), and the secondary outcomes were Timed Up and Go Test (TUGT), trunk flexor and extensor muscle strength, lumbopelvic stability, and lumbar multifidus muscle thickness measured pre- and post-intervention. Compared with participants in the control group, those in the experimental group showed significant improvement in NPRS, ODI, trunk extensor strength, lumbopelvic control, lumbar multifidus muscle thickness, and relative change in multifidus muscle thickness (significant time by group interactions, P < 0.05). Participants in both groups showed significant time effects (P < 0.001) for TUGT and trunk flexor strength outcome. Aquatic exercise combined with home exercise was superior to home exercise alone in reducing pain, disability and improving muscle strength, lumbopelvic stability, and lumbar multifidus muscle thickness.
    MeSH term(s) Humans ; Low Back Pain ; Postural Balance ; Time and Motion Studies ; Lumbosacral Region ; Exercise Therapy ; Muscle Strength/physiology
    Language English
    Publishing date 2023-07-03
    Publishing country England
    Document type Randomized Controlled Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-37237-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Biophysical and biochemical characterization of a recombinant Lyme disease vaccine antigen, CspZ-YA.

    Chen, Yi-Lin / Lee, Jungsoon / Liu, Zhuyun / Strych, Ulrich / Bottazzi, Maria Elena / Lin, Yi-Pin / Chen, Wen-Hsiang

    International journal of biological macromolecules

    2024  Volume 259, Issue Pt 2, Page(s) 129295

    Abstract: Lyme disease, caused by Lyme Borrelia spirochetes, is the most common vector-borne illness in the United States. Despite its global significance, with an estimated 14.5 % seroprevalence, there is currently no licensed vaccine. Previously, we demonstrated ...

    Abstract Lyme disease, caused by Lyme Borrelia spirochetes, is the most common vector-borne illness in the United States. Despite its global significance, with an estimated 14.5 % seroprevalence, there is currently no licensed vaccine. Previously, we demonstrated that CspZ-YA protein conferred protection against Lyme Borrelia infection, making it a promising vaccine candidate. However, such a protein was tagged with hexahistidine, and thus not preferred for vaccine development; furthermore, the formulation to stabilize the protein was understudied. In this work, we developed a two-step purification process for tag-free E. coli-expressed recombinant CspZ-YA. We further utilized various bioassays to analyze the protein and determine the suitable buffer system for long-term storage and formulation as a vaccine immunogen. The results indicated that a buffer with a pH between 6.5 and 8.5 stabilized CspZ-YA by reducing its surface hydrophobicity and colloidal interactions. Additionally, low pH values induced a change in local spatial conformation and resulted in a decrease in α-helix content. Lastly, an optimal salinity of 22-400 mM at pH 7.5 was found to be important for its stability. Collectively, this study provides a fundamental biochemical and biophysical understanding and insights into the ideal stabilizing conditions to produce CspZ-YA recombinant protein for use in vaccine formulation and development.
    MeSH term(s) Humans ; Lyme Disease Vaccines ; Borrelia burgdorferi ; Escherichia coli/genetics ; Seroepidemiologic Studies ; Lyme Disease/prevention & control ; Bacterial Outer Membrane Proteins/chemistry
    Chemical Substances Lyme Disease Vaccines ; Bacterial Outer Membrane Proteins
    Language English
    Publishing date 2024-01-09
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.129295
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Past, present, and future of Lyme disease vaccines: antigen engineering approaches and mechanistic insights.

    Chen, Wen-Hsiang / Strych, Ulrich / Bottazzi, Maria Elena / Lin, Yi-Pin

    Expert review of vaccines

    2022  Volume 21, Issue 10, Page(s) 1405–1417

    Abstract: Introduction: Transmitted by ticks, Lyme disease is the most common vector-borne disease in the Northern hemisphere. Despite the geographical expansion of human Lyme disease cases, no effective preventive strategies are currently available. Developing ... ...

    Abstract Introduction: Transmitted by ticks, Lyme disease is the most common vector-borne disease in the Northern hemisphere. Despite the geographical expansion of human Lyme disease cases, no effective preventive strategies are currently available. Developing an efficacious and safe vaccine is therefore urgently needed. Efforts have previously been taken to identify vaccine targets in the causative pathogen (
    Area covered: In this review, we summarize the antigen engineering approaches that have been applied to overcome those challenges and the underlying mechanisms that can be exploited to improve both safety and efficacy of future Lyme disease vaccines.
    Expert opinion: Over the past two decades, several new genetically redesigned Lyme disease vaccine candidates have shown success in both preclinical and clinical settings and built a solid foundation for further development. These studies have greatly informed the protective mechanisms of reducing Lyme disease burdens and ending the endemic of this disease.
    MeSH term(s) Animals ; Borrelia burgdorferi ; Humans ; Ixodes ; Lyme Disease/epidemiology ; Lyme Disease/prevention & control ; Lyme Disease Vaccines
    Chemical Substances Lyme Disease Vaccines
    Language English
    Publishing date 2022-07-22
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2181284-6
    ISSN 1744-8395 ; 1476-0584
    ISSN (online) 1744-8395
    ISSN 1476-0584
    DOI 10.1080/14760584.2022.2102484
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Recombinant protein vaccines, a proven approach against coronavirus pandemics.

    Pollet, Jeroen / Chen, Wen-Hsiang / Strych, Ulrich

    Advanced drug delivery reviews

    2021  Volume 170, Page(s) 71–82

    Abstract: With the COVID-19 pandemic now ongoing for close to a year, people all over the world are still waiting for a vaccine to become available. The initial focus of accelerated global research and development efforts to bring a vaccine to market as soon as ... ...

    Abstract With the COVID-19 pandemic now ongoing for close to a year, people all over the world are still waiting for a vaccine to become available. The initial focus of accelerated global research and development efforts to bring a vaccine to market as soon as possible was on novel platform technologies that promised speed but had limited history in the clinic. In contrast, recombinant protein vaccines, with numerous examples in the clinic for many years, missed out on the early wave of investments from government and industry. Emerging data are now surfacing suggesting that recombinant protein vaccines indeed might offer an advantage or complement to the nucleic acid or viral vector vaccines that will likely reach the clinic faster. Here, we summarize the current public information on the nature and on the development status of recombinant subunit antigens and adjuvants targeting SARS-CoV-2 infections.
    MeSH term(s) Animals ; COVID-19/epidemiology ; COVID-19/immunology ; COVID-19/prevention & control ; COVID-19 Vaccines/administration & dosage ; COVID-19 Vaccines/immunology ; Clinical Trials as Topic/methods ; Coronavirus Infections/epidemiology ; Coronavirus Infections/immunology ; Coronavirus Infections/prevention & control ; Drug Development/methods ; Health Resources/trends ; Humans ; Pandemics/prevention & control ; Recombinant Proteins/administration & dosage ; Recombinant Proteins/immunology ; SARS-CoV-2/drug effects ; SARS-CoV-2/immunology ; Vaccines, Synthetic/administration & dosage ; Vaccines, Synthetic/immunology ; Viral Vaccines/administration & dosage ; Viral Vaccines/immunology
    Chemical Substances COVID-19 Vaccines ; Recombinant Proteins ; Vaccines, Synthetic ; Viral Vaccines
    Language English
    Publishing date 2021-01-07
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 639113-8
    ISSN 1872-8294 ; 0169-409X
    ISSN (online) 1872-8294
    ISSN 0169-409X
    DOI 10.1016/j.addr.2021.01.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Effects of early aquatic exercise intervention on trunk strength and functional recovery of patients with lumbar fusion

    An-Hua Huang / Wen-Hsiang Chou / Wendy Tzyy-Jiuan Wang / Wen-Yin Chen / Yi-Fen Shih

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    a randomized controlled trial

    2023  Volume 8

    Abstract: Abstract This study investigated the effectiveness of an early aquatic exercise program on trunk muscle function and functional recovery of patients with lumbar fusion. Twenty-eight subjects were divided into two equal groups. Patients in the aquatic ... ...

    Abstract Abstract This study investigated the effectiveness of an early aquatic exercise program on trunk muscle function and functional recovery of patients with lumbar fusion. Twenty-eight subjects were divided into two equal groups. Patients in the aquatic group performed two 60-min aquatic exercise sessions and three 60-min home exercise sessions per week for 6 weeks, whereas those in the control group performed five sessions of 60-min home exercises per week for 6 weeks. The primary outcomes were the Numerical Pain Rating Scale (NPRS) and Oswestry Disability Index (ODI), and the secondary outcomes were Timed Up and Go Test (TUGT), trunk flexor and extensor muscle strength, lumbopelvic stability, and lumbar multifidus muscle thickness measured pre- and post-intervention. Compared with participants in the control group, those in the experimental group showed significant improvement in NPRS, ODI, trunk extensor strength, lumbopelvic control, lumbar multifidus muscle thickness, and relative change in multifidus muscle thickness (significant time by group interactions, P < 0.05). Participants in both groups showed significant time effects (P < 0.001) for TUGT and trunk flexor strength outcome. Aquatic exercise combined with home exercise was superior to home exercise alone in reducing pain, disability and improving muscle strength, lumbopelvic stability, and lumbar multifidus muscle thickness.
    Keywords Medicine ; R ; Science ; Q
    Subject code 796
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Potential for developing a SARS-CoV receptor-binding domain (RBD) recombinant protein as a heterologous human vaccine against coronavirus infectious disease (COVID)-19.

    Chen, Wen-Hsiang / Hotez, Peter J / Bottazzi, Maria Elena

    Human vaccines & immunotherapeutics

    2020  Volume 16, Issue 6, Page(s) 1239–1242

    Abstract: A SARS-CoV receptor-binding domain (RBD) recombinant protein was developed and manufactured under current good manufacturing practices in 2016. The protein, known as RBD219-N1 when formulated on Alhydrogel®, induced high-level neutralizing antibodies and ...

    Abstract A SARS-CoV receptor-binding domain (RBD) recombinant protein was developed and manufactured under current good manufacturing practices in 2016. The protein, known as RBD219-N1 when formulated on Alhydrogel®, induced high-level neutralizing antibodies and protective immunity with minimal immunopathology in mice after a homologous virus challenge with SARS-CoV (MA15 strain). We examined published evidence in support of whether the SARS-CoV RBD219-N1 could be repurposed as a heterologous vaccine against Coronavirus Infectious Disease (COVID)-19. Our findings include evidence that convalescent serum from SARS-CoV patients can neutralize SARS-CoV-2. Additionally, a review of published studies using monoclonal antibodies (mAbs) raised against SARS-CoV RBD and that neutralizes the SARS-CoV virus
    MeSH term(s) Betacoronavirus ; COVID-19 ; COVID-19 Vaccines ; Coronavirus Infections/immunology ; Coronavirus Infections/prevention & control ; Humans ; Pandemics/prevention & control ; Pneumonia, Viral/prevention & control ; Protein Domains ; Protein Subunits ; Recombinant Proteins/immunology ; SARS-CoV-2 ; Viral Envelope Proteins/chemistry ; Viral Envelope Proteins/immunology ; Viral Vaccines/immunology
    Chemical Substances COVID-19 Vaccines ; Protein Subunits ; Recombinant Proteins ; Viral Envelope Proteins ; Viral Vaccines
    Keywords covid19
    Language English
    Publishing date 2020-04-16
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2664176-8
    ISSN 2164-554X ; 2164-5515
    ISSN (online) 2164-554X
    ISSN 2164-5515
    DOI 10.1080/21645515.2020.1740560
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Proposal for Practical Approach in Prenatal Diagnosis of Beckwith–Wiedemann Syndrome and Review of the Literature

    Gwo-Chin Ma / Tze-Ho Chen / Wan-Ju Wu / Dong-Jay Lee / Wen-Hsiang Lin / Ming Chen

    Diagnostics, Vol 12, Iss 7, p

    2022  Volume 1709

    Abstract: Background: Beckwith–Wiedemann syndrome (BWS) is a phenotypically and genetically heterogeneous disorder associated with epigenetic/genetic aberrations on chromosome 11p15.4p15.5. There is no consensus criterion for prenatal diagnosis of BWS. Methods: ... ...

    Abstract Background: Beckwith–Wiedemann syndrome (BWS) is a phenotypically and genetically heterogeneous disorder associated with epigenetic/genetic aberrations on chromosome 11p15.4p15.5. There is no consensus criterion for prenatal diagnosis of BWS. Methods: Three BWS patients with their clinical histories, prenatal ultrasonographic features, and results of molecular diagnosis were presented. Likewise, by incorporating the findings of our cases and literature review, the phenotypic spectrum and genotype–phenotype correlations of fetal BWS were summarized, and a practical approach in prenatal diagnosis of BWS was proposed. Results: A total of 166 BWS cases with prenatal features were included for analysis. Common fetal features include abdominal wall defects (42.8%), polyhydramnios (33.1%), and macrosomia (32.5%). Molecular pathologies include methylation changes in imprinting control region 1 and 2 (ICR1 and ICR2), paternal uniparental disomy of chromosome 11p15.5, copy number change involving 11p15, etc. Some genotype–phenotype correlations were observed. However, the broad phenotypic spectrum but limited features manifested by affected fetuses rendering ultrasonographic diagnosis not easy. Conclusions: Molecular tests are used for prenatal diagnosis of BWS suspected by ultrasonography. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is recommended as the first-line molecular tool because it simultaneously detects ICR1/ICR2 methylation statuses and copy numbers that solve the majority of clinical cases in the prenatal scenario.
    Keywords BWS ; ultrasonography ; imprinting ; uniparental disomy ; MS-MLPA ; ICR1 ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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