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  1. Article ; Online: Ionome mapping and amino acid metabolome profiling of Phaseolus vulgaris L. seeds imbibed with computationally informed phytoengineered copper sulphide nanoparticles.

    Botha, Nandipha L / Cloete, Karen J / Šmit, Žiga / Isaković, Kristina / Akbari, Mahmood / Morad, Razieh / Madiba, Itani / David, Oladipupo Moyinoluwa / Santos, Luis P M / Dube, Admire / Pelicon, Primoz / Maaza, Malik

    Discover nano

    2024  Volume 19, Issue 1, Page(s) 8

    Abstract: This study reports the effects of a computationally informed and avocado-seed mediated Phyto engineered CuS nanoparticles as fertilizing agent on the ionome and amino acid metabolome of Pinto bean seeds using both bench top and ion beam analytical ... ...

    Abstract This study reports the effects of a computationally informed and avocado-seed mediated Phyto engineered CuS nanoparticles as fertilizing agent on the ionome and amino acid metabolome of Pinto bean seeds using both bench top and ion beam analytical techniques. Physico-chemical analysis of the Phyto engineered nanoparticles with scanning-electron microscopy, transmission electron microscopy, X-ray diffraction, and Fourier Transform Infrared Spectroscopy confirmed the presence of CuS nanoparticles. Molecular dynamics simulations to investigate the interaction of some active phytocompounds in avocado seeds that act as reducing agents with the nano-digenite further showed that 4-hydroxybenzoic acid had a higher affinity for interacting with the nanoparticle's surface than other active compounds. Seeds treated with the digenite nanoparticles exhibited a unique ionome distribution pattern as determined with external beam proton-induced X-ray emission, with hotspots of Cu and S appearing in the hilum and micropyle area that indicated a possible uptake mechanism via the seed coat. The nano-digenite also triggered a plant stress response by slightly altering seed amino acid metabolism. Ultimately, the nano-digenite may have important implications as a seed protective or nutritive agent as advised by its unique distribution pattern and effect on amino acid metabolism.
    Language English
    Publishing date 2024-01-04
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2731-9229
    ISSN (online) 2731-9229
    DOI 10.1186/s11671-023-03953-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Thesis: Einfluss von Rebsorte, Stickstoffdüngung und Traubenfäulnis auf die Bildung biogener Amine in Beeren, Most und Wein der Rebe (Vitis vinifera L.)

    Smit, Inga

    (Geisenheimer Berichte ; 64)

    2009  

    Author's details Inga Smit
    Series title Geisenheimer Berichte ; 64
    Collection
    Subject code 634.88
    Language German
    Size IX, 188 S. : Ill., graph. Darst.
    Publisher Ges. zur Förderung der Forschungsanst. Geisenheim
    Publishing place Geisenheim
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Hannover, Univ., Diss., 2008
    Note Zsfassung in engl. Sprache
    HBZ-ID HT015957489
    ISBN 978-3-934742-53-6 ; 3-934742-53-X
    Database Catalogue ZB MED Nutrition, Environment, Agriculture

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  3. Article ; Online: The effect of potassium fertilization on the metabolite profile of tomato fruit (Solanum lycopersicum L.).

    Weinert, Christoph H / Sonntag, Frederike / Egert, Björn / Pawelzik, Elke / Kulling, Sabine E / Smit, Inga

    Plant physiology and biochemistry : PPB

    2020  Volume 159, Page(s) 89–99

    Abstract: The macronutrient potassium (K) has vital physiological functions in plants and its availability can strongly impact quality of crops like tomato. The impact of K nutrition on conventional tomato fruit quality parameters has been described several times, ...

    Abstract The macronutrient potassium (K) has vital physiological functions in plants and its availability can strongly impact quality of crops like tomato. The impact of K nutrition on conventional tomato fruit quality parameters has been described several times, but detailed investigations on the effect of K supply on the fruit metabolite profile are still rare. To fill this gap, we investigated the influence of K fertilization on the metabolite profile of tomato fruits. For this purpose, an outdoor pot experiment with three different cocktail tomato cultivars was performed. A fertilization regimen with five K levels was applied, ranging from deficiency to sufficient supply. Fruit samples were analyzed by untargeted GC×GC-MS to cover the primary metabolite profile as well as some secondary metabolites. As verified using ICP-OES, fruit K content was highly proportional to the supplied amount of K. At the metabolite profile level, the most prominent and cultivar-independent effect of increased K fertilization was the rise of tricarboxylic acid (TCA) cycle intermediates. Further effects were more cultivar-specific, for example an increase of the mobile nitrogen pool (e.g. amines like putrescine and amides like asparagine), changes in the profile of minor sugars (especially disaccharides) as well as higher levels of some secondary metabolites. Pronounced response patterns were mainly observed in the cultivars Primavera and Yellow Submarine that were recently characterized as higher yielding, demanding a stronger consideration of cultivar differences in future studies.
    Language English
    Publishing date 2020-12-13
    Publishing country France
    Document type Journal Article
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2020.12.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: How to Dose Vancomycin in Overweight and Obese Patients with Varying Renal (Dys)function in the Novel Era of AUC 400-600 mg·h/L-Targeted Dosing.

    Zhang, Tan / Krekels, Elke H J / Smit, Cornelis / van Dongen, Eric P A / Brüggemann, Roger J M / Knibbe, Catherijne A J

    Clinical pharmacokinetics

    2023  Volume 63, Issue 1, Page(s) 79–91

    Abstract: ... h/L in the first 48 h, two loading doses are required for both continuous infusion and intermittent ...

    Abstract Background and objective: The latest vancomycin guideline recommends area under the curve (AUC)-targeted dosing and monitoring for efficacy and safety. However, guidelines for AUC-targeted starting dosing in patients with obesity and/or renal insufficiency are currently lacking. This study quantifies the pharmacokinetics (PK) of vancomycin in this population and provides AUC-targeted dosing recommendations.
    Methods: Vancomycin concentrations (n = 1188) from therapeutic drug monitoring of 210 overweight and obese patients with varying degrees of renal (dys)function from the ward (74.8%) and intensive care unit (ICU, 25.2%) were pooled with published rich concentration-time data (n = 207) from 20 (morbidly) obese subjects undergoing bariatric surgery. A population model was developed using NONMEM 7.4. Stochastic simulations were performed to design dosing guidelines targeting an AUC
    Results: Vancomycin clearance (CL) was found to increase linearly with total bodyweight and with renal function (CKD-EPI) in a power relation. Additionally, CL proved 15.5% lower in ICU patients. Our model shows that, to reach the target AUC between 400 and 600 mg·h/L in the first 48 h, two loading doses are required for both continuous infusion and intermittent dosing regimens. Maintenance doses were found to require adjustment for total bodyweight, renal function, and ICU admission status. With this guideline, the median AUC
    Conclusions: To achieve safe and effective vancomycin exposure for maintenance doses in overweight and obese patients, renal function, total bodyweight, and ICU admission status should be taken into account.
    Trial registration: The AMIGO trial was registered in the Dutch Trial Registry [NTR6058].
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacokinetics ; Area Under Curve ; Kidney ; Obesity/complications ; Obesity/drug therapy ; Overweight/complications ; Overweight/drug therapy ; Vancomycin/pharmacokinetics
    Chemical Substances Anti-Bacterial Agents ; Vancomycin (6Q205EH1VU)
    Language English
    Publishing date 2023-11-16
    Publishing country Switzerland
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 197627-8
    ISSN 1179-1926 ; 0312-5963
    ISSN (online) 1179-1926
    ISSN 0312-5963
    DOI 10.1007/s40262-023-01324-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The effect of potassium fertilization on the metabolite profile of tomato fruit (Solanum lycopersicum L.)

    Weinert, Christoph H / Sonntag, Frederike / Egert, Björn / Pawelzik, Elke / Kulling, Sabine E / Smit, Inga

    Plant physiology and biochemistry. 2021 Feb., v. 159

    2021  

    Abstract: The macronutrient potassium (K) has vital physiological functions in plants and its availability can strongly impact quality of crops like tomato. The impact of K nutrition on conventional tomato fruit quality parameters has been described several times, ...

    Abstract The macronutrient potassium (K) has vital physiological functions in plants and its availability can strongly impact quality of crops like tomato. The impact of K nutrition on conventional tomato fruit quality parameters has been described several times, but detailed investigations on the effect of K supply on the fruit metabolite profile are still rare. To fill this gap, we investigated the influence of K fertilization on the metabolite profile of tomato fruits. For this purpose, an outdoor pot experiment with three different cocktail tomato cultivars was performed. A fertilization regimen with five K levels was applied, ranging from deficiency to sufficient supply. Fruit samples were analyzed by untargeted GC×GC-MS to cover the primary metabolite profile as well as some secondary metabolites. As verified using ICP-OES, fruit K content was highly proportional to the supplied amount of K. At the metabolite profile level, the most prominent and cultivar-independent effect of increased K fertilization was the rise of tricarboxylic acid (TCA) cycle intermediates. Further effects were more cultivar-specific, for example an increase of the mobile nitrogen pool (e.g. amines like putrescine and amides like asparagine), changes in the profile of minor sugars (especially disaccharides) as well as higher levels of some secondary metabolites. Pronounced response patterns were mainly observed in the cultivars Primavera and Yellow Submarine that were recently characterized as higher yielding, demanding a stronger consideration of cultivar differences in future studies.
    Keywords Solanum lycopersicum ; amides ; asparagine ; cultivars ; fruit quality ; fruits ; nitrogen ; nutrition ; plant physiology ; potassium ; putrescine ; secondary metabolites ; tomatoes
    Language English
    Dates of publication 2021-02
    Size p. 89-99.
    Publishing place Elsevier Masson SAS
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2020.12.010
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Phytomass and ecological significance of Chrysocoma ciliata L. within the Lets’eng-la-Letsie catchment area of Lesotho, southern Africa

    Smit, GN / Janse van Rensburg, G

    African journal of range & forage science. 2021 Mar. 12, v. 38, no. 1

    2021  

    Abstract: The Lets’eng-la-Letsie wetland is an official Ramsar site, but the wetland and upland catchment areas suffer from overgrazing, erosion and over exploitation. Chrysocoma ciliata has a reputation as an unpalatable invader and is particularly common on the ... ...

    Abstract The Lets’eng-la-Letsie wetland is an official Ramsar site, but the wetland and upland catchment areas suffer from overgrazing, erosion and over exploitation. Chrysocoma ciliata has a reputation as an unpalatable invader and is particularly common on the drier northern slopes. The objectives of the study were to quantify the phytomass of this shrub with the aid of a developed allometric phytomass quantification technique and to evaluate the ecological significance of this shrub within the area. Highly significant (p < 0.001) positive regressions with coefficients of determinations as high as r ² = 0.94 between phytomass and plant canopy diameter were achieved. The upland slopes support a high density of more than 35 000 C. ciliata plants ha⁻¹ with a phytomass of more than 3 600 kg DM ha⁻¹ and a correspondingly low herbaceous phytomass of 446.57 kg DM ha⁻¹. The edible parts of the plants (flowers, leaves and shoots <2.0 mm in diameter) were estimated to be 1 198 kg DM ha⁻¹, with a crude protein content of 8.84%. There was evidence that C. ciliata is intolerant of wet conditions and the degradation of the catchment areas will result in drier soil profiles, which will favour the further spread of this species.
    Keywords allometry ; canopy ; crude protein ; forage and feed science ; highlands ; phytomass ; shrubs ; watersheds ; wetlands ; Lesotho
    Language English
    Dates of publication 2021-0312
    Size p. 102-109.
    Publishing place Taylor & Francis
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1160792-0
    ISSN 1727-9380 ; 1022-0119 ; 0256-6702
    ISSN (online) 1727-9380
    ISSN 1022-0119 ; 0256-6702
    DOI 10.2989/10220119.2020.1853605
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: The effect of potassium fertilization on the metabolite profile of tomato fruit (Solanum lycopersicum L.)

    Weinert, Christoph H. / Sonntag, Frederike / Egert, Björn / Pawelzik, Elke / Kulling, Sabine E. / Smit, Inga

    2020  

    Keywords Text ; ddc:660 ; Tomato fruit quality -- Potassium -- Minerals -- TCA cycle -- GC×GC-MS metabolomics
    Language English
    Publishing date 2020-12-13
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Book ; Online ; Thesis: Investigations on flowering time genes from oilseed rape (Brassica napus L.)

    Shah, Smit [Verfasser]

    2019  

    Author's details Smit Shah
    Keywords Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
    Subject code sg630
    Language English
    Publisher Universitätsbibliothek Kiel
    Publishing place Kiel
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  9. Article ; Online: Ileal digestibility of energy and amino acids in three faba bean cultivars (Vicia faba L.) planted and harvested early or late in broiler chickens.

    Smit, Miranda N / Ketelaar, Robin F / He, Liangfei / Beltranena, Eduardo

    Poultry science

    2021  Volume 100, Issue 9, Page(s) 101332

    Abstract: A concern of both pulse growers and poultry producers is how the timing of planting and harvesting affect the nutritional quality of faba bean for broiler chickens. To investigate, half of the seed of 2 zero-tannin cultivars (Snowbird and Snowdrop) and 1 ...

    Abstract A concern of both pulse growers and poultry producers is how the timing of planting and harvesting affect the nutritional quality of faba bean for broiler chickens. To investigate, half of the seed of 2 zero-tannin cultivars (Snowbird and Snowdrop) and 1 low vicine and convicine cultivar (Fabelle) were planted at a single site either in early May and harvested in late September (EARLY) or planted in late May and harvested in late October (LATE). Diets of the 3 EARLY or LATE cultivars (95% inclusion) were fed to 756 broiler chickens (Ross 708) from d 15. Chickens were housed in 56 floor pens (13-14 birds/pen) in a 3 × 2 factorial arrangement (7 pens/diet). A nitrogen-free diet to correct for endogenous amino acid (AA) losses was fed to broilers in 14 pens from d 20. Ileal digesta was collected after euthanizing birds on day 23 or 24. Planting and harvesting LATE vs. EARLY increased the proportion of immature beans from 5 to 64% for Snowbird, 7 to 79% for Snowdrop, and 22 to 80% for Fabelle. Planting and harvesting LATE vs. EARLY increased the proportion of frost-damaged beans from 20 to 83% for Snowbird, 36 to 88% for Snowdrop, and 5 to 29% for Fabelle. Planting and harvesting LATE vs. EARLY increased (P < 0.001) apparent ileal digestibility (AID) of gross energy (GE) by 45% and standardized ileal digestibility (SID) of CP by 13%. Planting and harvesting LATE vs. EARLY increased (P < 0.001) SID of AA by 11% except Cys. Snowbird and Snowdrop had greater (P < 0.05) SID of AA by 4.5% except Thr and Trp compared with Fabelle. Fabelle had 13% greater (P < 0.001) SID of Trp compared with Snowbird or Snowdrop. Results indicate that planting and harvesting LATE vs. EARLY increased GE, CP, and AA digestibility possibly by frost interrupting bean ripening on the field. Hull tannin content may have reduced the AA digestibility of Fabelle compared with Snowbird or Snowdrop.
    MeSH term(s) Amino Acids ; Animal Feed/analysis ; Animal Nutritional Physiological Phenomena ; Animals ; Chickens ; Diet/veterinary ; Digestion ; Ileum ; Vicia faba
    Chemical Substances Amino Acids
    Language English
    Publishing date 2021-06-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 242586-5
    ISSN 1525-3171 ; 0032-5791
    ISSN (online) 1525-3171
    ISSN 0032-5791
    DOI 10.1016/j.psj.2021.101332
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Enhancing faba bean (Vicia faba L.) genome resources.

    Cooper, James W / Wilson, Michael H / Derks, Martijn F L / Smit, Sandra / Kunert, Karl J / Cullis, Christopher / Foyer, Christine H

    Journal of experimental botany

    2017  Volume 68, Issue 8, Page(s) 1941–1953

    Abstract: Grain legume improvement is currently impeded by a lack of genomic resources. The paucity of genome information for faba bean can be attributed to the intrinsic difficulties of assembling/annotating its giant (~13 Gb) genome. In order to address this ... ...

    Abstract Grain legume improvement is currently impeded by a lack of genomic resources. The paucity of genome information for faba bean can be attributed to the intrinsic difficulties of assembling/annotating its giant (~13 Gb) genome. In order to address this challenge, RNA-sequencing analysis was performed on faba bean (cv. Wizard) leaves. Read alignment to the faba bean reference transcriptome identified 16 300 high quality unigenes. In addition, Illumina paired-end sequencing was used to establish a baseline for genomic information assembly. Genomic reads were assembled de novo into contigs with a size range of 50-5000 bp. Over 85% of sequences did not align to known genes, of which ~10% could be aligned to known repetitive genetic elements. Over 26 000 of the reference transcriptome unigenes could be aligned to DNA-sequencing (DNA-seq) reads with high confidence. Moreover, this comparison identified 56 668 potential splice points in all identified unigenes. Sequence length data were extended at 461 putative loci through alignment of DNA-seq contigs to full-length, publicly available linkage marker sequences. Reads also yielded coverages of 3466× and 650× for the chloroplast and mitochondrial genomes, respectively. Inter- and intraspecies organelle genome comparisons established core legume organelle gene sets, and revealed polymorphic regions of faba bean organelle genomes.
    Language English
    Publishing date 2017-04-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2976-2
    ISSN 1460-2431 ; 0022-0957
    ISSN (online) 1460-2431
    ISSN 0022-0957
    DOI 10.1093/jxb/erx117
    Database MEDical Literature Analysis and Retrieval System OnLINE

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