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Article ; Online: Suprachoroidal spheroidal mesenchymal stem cell implantation in retinitis pigmentosa: clinical results of 6 months follow-up.

Özkan, Berna / Yılmaz Tuğan, Büşra / Hemşinlioğlu, Cansu / Sır Karakuş, Gözde / Şahin, Özlem / Ovalı, Ercüment

2023 Volume 14, Issue 1, Page(s) 252

Abstract: Purpose: This prospective clinical case series aimed to evaluate the effect of suprachoroidal implantation of mesenchymal stem cells (MSCs) in the form of spheroids as a stem cell therapy for retinitis pigmentosa (RP) patients with relatively good ... ...

Abstract	Purpose: This prospective clinical case series aimed to evaluate the effect of suprachoroidal implantation of mesenchymal stem cells (MSCs) in the form of spheroids as a stem cell therapy for retinitis pigmentosa (RP) patients with relatively good visual acuity. Methods: Fifteen eyes of 15 patients with RP who received suprachoroidal implantation of MSCs in the form of spheroids were included. Best-corrected visual acuity (BCVA), 10-2 and 30-2 visual field examination and multifocal electroretinography (mfERG) recordings were recorded at baseline, postoperative 1st, 3rd and 6th months during follow-up. Results: Baseline median BCVA of RP patients was 1.30 (1.00-2.00) logMAR. BCVA has improved to 1.00 (0.50-1.30), 0.80 (0.40-1.30) and 0.80 (0.40-1.30) at the postoperative 1st, 3rd and 6th months, respectively. The improvements from baseline to the 3rd and 6th months were statistically significant (p = 0.03 and p < 0.001, respectively). In the 30-2 VF test, median MD was significantly improved at the 6th month compared to baseline (p = 0.030). In the 10-2 VF test, the median MD value was significantly different at the 6th month compared to the baseline (p = 0.043). The PSD value of the 10-2 VF test was significantly different at the 6th month compared to the 3rd month (p = 0.043). The amplitudes of P1 waves in < 2°, 5°-10° and 10°-15° rings improved significantly at the postoperative 6th month (p = 0.014, p = 0.018 and p = 0.017, respectively). There was also a statistically significant improvement in implicit times of P1 waves in 10°-15° ring at the postoperative 6th month (p = 0.004). Conclusion: Suprachoroidal implantation of MSCs in the form of spheroids as a stem cell therapy for RP patients with relatively good visual acuity has an improving effect on BCVA, VF and mfERG recordings during the 6-month follow-up period. Spheroidal MSCs with enhanced effects may be more successful in preventing apoptosis and improving retinal tissue healing in RP patients.
MeSH term(s)	Humans ; Follow-Up Studies ; Prospective Studies ; Embryo Implantation ; Retinitis Pigmentosa/therapy ; Mesenchymal Stem Cells
Language	English
Publishing date	2023-09-13
Publishing country	England
Document type	Journal Article
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-023-03489-z
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Article ; Online: microRNA 1307 Is a Potential Target for SARS-CoV-2 Infection: An

Arisan, Elif Damla / Dart, D Alwyn / Grant, Guy H / Dalby, Andrew / Kancagi, Derya Dilek / Turan, Raife Dilek / Yurtsever, Bulut / Karakus, Gozde Sir / Ovali, Ercument / Lange, Sigrun / Uysal-Onganer, Pinar

ACS omega

2022 Volume 7, Issue 42, Page(s) 38003–38014

Abstract: microRNAs (miRs) are proposed as critical molecular targets in SARS-CoV-2 infection. Our ... ...

Abstract	microRNAs (miRs) are proposed as critical molecular targets in SARS-CoV-2 infection. Our recent
Language	English
Publishing date	2022-10-11
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.2c05245
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Article ; Online: Mutational landscape of SARS-CoV-2 genome in Turkey and impact of mutations on spike protein structure.

Hatirnaz Ng, Ozden / Akyoney, Sezer / Sahin, Ilayda / Soykam, Huseyin Okan / Bayram Akcapinar, Gunseli / Ozdemir, Ozkan / Kancagi, Derya Dilek / Sir Karakus, Gozde / Yurtsever, Bulut / Kocagoz, Ayse Sesin / Ovali, Ercument / Ozbek, Ugur

PloS one

2021 Volume 16, Issue 12, Page(s) e0260438

Abstract: The Coronavirus Disease 2019 (COVID-19) was declared a pandemic in March 2020 by the World Health Organization (WHO). As of May 25th, 2021 there were 2.059.941 SARS-COV2 genome sequences that have been submitted to the GISAID database, with numerous ... ...

Abstract	The Coronavirus Disease 2019 (COVID-19) was declared a pandemic in March 2020 by the World Health Organization (WHO). As of May 25th, 2021 there were 2.059.941 SARS-COV2 genome sequences that have been submitted to the GISAID database, with numerous variations. Here, we aim to analyze the SARS-CoV-2 genome data submitted to the GISAID database from Turkey and to determine the variant and clade distributions by the end of May 2021, in accordance with their appearance timeline. We compared these findings to USA, Europe, and Asia data as well. We have also evaluated the effects of spike protein variations, detected in a group of genome sequences of 13 patients who applied to our clinic, by using 3D modeling algorithms. For this purpose, we analyzed 4607 SARS-CoV-2 genome sequences submitted by different lab centers from Turkey to the GISAID database between March 2020 and May 2021. Described mutations were also introduced in silico to the spike protein structure to analyze their isolated impacts on the protein structure. The most abundant clade was GR followed by G, GH, and GRY and we did not detect any V clade. The most common variant was B.1, followed by B.1.1, and the UK variant, B.1.1.7. Our results clearly show a concordance between the variant distributions, the number of cases, and the timelines of different variant accumulations in Turkey. The 3D simulations indicate an increase in the surface hydrophilicity of the reference spike protein and the detected mutations. There was less surface hydrophilicity increase in the Asp614Gly mutation, which exhibits a more compact conformation around the ACE-2 receptor binding domain region, rendering the structure in a "down" conformation. Our genomic findings can help to model vaccination programs and protein modeling may lead to different approaches for COVID-19 treatment strategies.
MeSH term(s)	Adult ; Aged ; Algorithms ; COVID-19/pathology ; COVID-19/virology ; Female ; Genome, Viral ; Humans ; Male ; Middle Aged ; Molecular Dynamics Simulation ; Mutation ; Phylogeny ; Protein Domains/genetics ; SARS-CoV-2/classification ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/metabolism ; Spike Glycoprotein, Coronavirus/chemistry ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/metabolism ; Turkey ; Young Adult
Chemical Substances	Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2021-12-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0260438
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Article ; Online: The effects of thymoquinone on pancreatic cancer and immune cells.

Alandağ, Celal / Kancaği, Derya Dilek / Karakuş Sir, Gözde / Çakirsoy, Didem / Ovali, Ercüment / Karaman, Elanur / Yüce, Elif / Özdemir, Feyyaz

Revista da Associacao Medica Brasileira (1992)

2022 Volume 68, Issue 8, Page(s) 1023–1026

Abstract: Objectives: Black cumin is widely used as a spice and as a traditional treatment. The active ingredient in black cumin seeds is thymoquinone. Thymoquinone has shown anticancer effects in some cancers. We planned to investigate its anticancer effect on ... ...

Abstract	Objectives: Black cumin is widely used as a spice and as a traditional treatment. The active ingredient in black cumin seeds is thymoquinone. Thymoquinone has shown anticancer effects in some cancers. We planned to investigate its anticancer effect on pancreatic cancer cell lines. Methods: Thymoquinone chemical component in various doses was prepared and inoculated on pancreatic cancer cell culture, healthy mesenchymal stem cells, and peripheral blood mononuclear cell culture. IC50 values were calculated by absorbance data and measuring cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide staining of cells incubated with thymoquinone at 24, 48, and 72 h. Results: There was dose-related cytotoxicity. Maximal cytotoxicity was observed at 24 h and 100 μM thymoquinone concentrations in pancreatic cancer cell culture and mesenchymal stem cells. Any concentration of thymoquinone was not cytotoxic to peripheral blood mononuclear cell. Thymoquinone even caused proliferation at a concentration of 6.25 μM. Conclusions: Since the cytotoxic concentration of thymoquinone on pancreatic cancer cell culture and mesenchymal stem cells is the same, it is not appropriate to use thymoquinone to achieve cytotoxicity in pancreatic cancer. However, since thymoquinone provides proliferation in peripheral blood mononuclear cell at a noncytotoxic dose, it may have an immune activator effect. Therefore, in vivo studies are needed to investigate the effect of thymoquinone on the immune system.
MeSH term(s)	Antineoplastic Agents/therapeutic use ; Benzoquinones/pharmacology ; Benzoquinones/therapeutic use ; Bromides/therapeutic use ; Humans ; Leukocytes, Mononuclear/metabolism ; Nigella sativa ; Pancreatic Neoplasms/drug therapy ; Pancreatic Neoplasms
Chemical Substances	Antineoplastic Agents ; Benzoquinones ; Bromides ; thymoquinone (O60IE26NUF)
Language	English
Publishing date	2022-08-31
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	731969-1
ISSN	1806-9282 ; 0104-4230 ; 0004-5241 ; 0102-843X
ISSN (online)	1806-9282
ISSN	0104-4230 ; 0004-5241 ; 0102-843X
DOI	10.1590/1806-9282.20220066
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Article ; Online: Mutational landscape of SARS-CoV-2 genome in Turkey and impact of mutations on spike protein structure.

Ozden Hatirnaz Ng / Sezer Akyoney / Ilayda Sahin / Huseyin Okan Soykam / Gunseli Bayram Akcapinar / Ozkan Ozdemir / Derya Dilek Kancagi / Gozde Sir Karakus / Bulut Yurtsever / Ayse Sesin Kocagoz / Ercument Ovali / Ugur Ozbek

PLoS ONE, Vol 16, Iss 12, p e

2021 Volume 0260438

Abstract	The Coronavirus Disease 2019 (COVID-19) was declared a pandemic in March 2020 by the World Health Organization (WHO). As of May 25th, 2021 there were 2.059.941 SARS-COV2 genome sequences that have been submitted to the GISAID database, with numerous variations. Here, we aim to analyze the SARS-CoV-2 genome data submitted to the GISAID database from Turkey and to determine the variant and clade distributions by the end of May 2021, in accordance with their appearance timeline. We compared these findings to USA, Europe, and Asia data as well. We have also evaluated the effects of spike protein variations, detected in a group of genome sequences of 13 patients who applied to our clinic, by using 3D modeling algorithms. For this purpose, we analyzed 4607 SARS-CoV-2 genome sequences submitted by different lab centers from Turkey to the GISAID database between March 2020 and May 2021. Described mutations were also introduced in silico to the spike protein structure to analyze their isolated impacts on the protein structure. The most abundant clade was GR followed by G, GH, and GRY and we did not detect any V clade. The most common variant was B.1, followed by B.1.1, and the UK variant, B.1.1.7. Our results clearly show a concordance between the variant distributions, the number of cases, and the timelines of different variant accumulations in Turkey. The 3D simulations indicate an increase in the surface hydrophilicity of the reference spike protein and the detected mutations. There was less surface hydrophilicity increase in the Asp614Gly mutation, which exhibits a more compact conformation around the ACE-2 receptor binding domain region, rendering the structure in a "down" conformation. Our genomic findings can help to model vaccination programs and protein modeling may lead to different approaches for COVID-19 treatment strategies.
Keywords	Medicine ; R ; Science ; Q
Subject code	612
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Mutational landscape of SARS-CoV-2 genome in Turkey and impact of mutations on spike protein structure

PLoS ONE, Vol 16, Iss

2021 Volume 12

Abstract	The Coronavirus Disease 2019 (COVID-19) was declared a pandemic in March 2020 by the World Health Organization (WHO). As of May 25th, 2021 there were 2.059.941 SARS-COV2 genome sequences that have been submitted to the GISAID database, with numerous variations. Here, we aim to analyze the SARS-CoV-2 genome data submitted to the GISAID database from Turkey and to determine the variant and clade distributions by the end of May 2021, in accordance with their appearance timeline. We compared these findings to USA, Europe, and Asia data as well. We have also evaluated the effects of spike protein variations, detected in a group of genome sequences of 13 patients who applied to our clinic, by using 3D modeling algorithms. For this purpose, we analyzed 4607 SARS-CoV-2 genome sequences submitted by different lab centers from Turkey to the GISAID database between March 2020 and May 2021. Described mutations were also introduced in silico to the spike protein structure to analyze their isolated impacts on the protein structure. The most abundant clade was GR followed by G, GH, and GRY and we did not detect any V clade. The most common variant was B.1, followed by B.1.1, and the UK variant, B.1.1.7. Our results clearly show a concordance between the variant distributions, the number of cases, and the timelines of different variant accumulations in Turkey. The 3D simulations indicate an increase in the surface hydrophilicity of the reference spike protein and the detected mutations. There was less surface hydrophilicity increase in the Asp614Gly mutation, which exhibits a more compact conformation around the ACE-2 receptor binding domain region, rendering the structure in a “down” conformation. Our genomic findings can help to model vaccination programs and protein modeling may lead to different approaches for COVID-19 treatment strategies.
Keywords	Medicine ; R ; Science ; Q
Subject code	612
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: In Vitro FVIII-Encoding Transgenic Mesenchymal Stem Cells Maintain Successful Coagulation in FVIII-Deficient Plasma Mimicking Hemophilia A

Hemşinlioğlu, Cansu / Aslan, Elif Sibel / Taştan, Cihan / Çakırsoy, Didem / Turan, Raife Dilek / Seyis, Utku / Elek, Muhammer / Sır Karakuş, Gözde / Günaydın, Ömur Selin / Abanuz, Selen / Kançağı, Derya Dilek / Yurtsever, Bulut / Yalçın, Koray / Kasap, Murat / Ovalı, Ercüment

Turkish journal of haematology : official journal of Turkish Society of Haematology

2023 Volume 40, Issue 2, Page(s) 118–124

Abstract: Objective: Hemophilia A is an X-linked recessive bleeding disorder caused by a deficiency of plasma coagulation factor VIII (FVIII), and it accounts for about 80%-85% of all cases of hemophilia. Plasma-derived therapies or recombinant FVIII concentrates ...

Abstract	Objective: Hemophilia A is an X-linked recessive bleeding disorder caused by a deficiency of plasma coagulation factor VIII (FVIII), and it accounts for about 80%-85% of all cases of hemophilia. Plasma-derived therapies or recombinant FVIII concentrates are used to prevent and treat the bleeding symptoms along with FVIII-mimicking antibodies. Recently, the European Medicines Agency granted conditional marketing approval for the first gene therapy for hemophilia A. The aim of this study was to determine the effectiveness of coagulation in correcting FVIII deficiency with FVIII-secreting transgenic mesenchymal stem cells (MSCs). Materials and methods: A lentiviral vector encoding a B domain-deleted FVIII cDNA sequence with CD45R0 truncated (CD45R0t) surface marker was designed to develop a transgenic FVIII-expressing primary cell line by transducing MSCs. The efficacy and functionality of the FVIII secreted from the MSCs was assessed with anti-FVIII ELISA, CD45R0t flow cytometry, FVIII western blot, and mixing test analysis in vitro. Results: The findings of this study showed that the transgenic MSCs maintained persistent FVIII secretion. There was no significant difference in FVIII secretion over time, suggesting stable FVIII expression from the MSCs. The functionality of the FVIII protein secreted in the MSC supernatant was demonstrated by applying a mixing test in coagulation analysis. In the mixing test analysis, FVIII-deficient human plasma products were mixed with either a saline control or FVIII-secreted MSC supernatant. The mean FVIII level of the saline control group was 0.41±0.03 IU/dL, whereas the mean level was 25.41±33.38 IU/dL in the FVIII-secreting MSC supernatant mixed group (p<0.01). The mean activated partial thromboplastin time (aPTT) of the saline control group was 92.69±11.38 s, while in the FVIII-secreting MSC supernatant mixed group, the mean aPTT level decreased to 38.60±13.38 s (p<0.001). Conclusion: The findings of this in vitro study suggest that the new method presented here is promising as a possible treatment for hemophilia A. Accordingly, a study of FVIII-secreting transgenic MSCs will next be initiated in a FVIII-knockout animal model.
MeSH term(s)	Animals ; Humans ; Factor VIII/genetics ; Hemophilia A/genetics ; Hemophilia A/therapy ; Blood Coagulation ; Genetic Therapy/methods ; Mesenchymal Stem Cells/metabolism
Chemical Substances	Factor VIII (9001-27-8)
Language	English
Publishing date	2023-04-06
Publishing country	Turkey
Document type	Journal Article
ZDB-ID	2185903-6
ISSN	1308-5263 ; 1300-7777
ISSN (online)	1308-5263
ISSN	1300-7777
DOI	10.4274/tjh.galenos.2023.2022-0318
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Article ; Online: Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice.

Turan, Raife Dilek / Tastan, Cihan / Dilek Kancagi, Derya / Yurtsever, Bulut / Sir Karakus, Gozde / Ozer, Samed / Abanuz, Selen / Cakirsoy, Didem / Tumentemur, Gamze / Demir, Sevda / Seyis, Utku / Kuzay, Recai / Elek, Muhammer / Kocaoglu, Miyase Ezgi / Ertop, Gurcan / Arbak, Serap / Acikel Elmas, Merve / Hemsinlioglu, Cansu / Hatirnaz Ng, Ozden /

Akyoney, Sezer / Sahin, Ilayda / Kayhan, Cavit Kerem / Tokat, Fatma / Akpinar, Gurler / Kasap, Murat / Kocagoz, Ayse Sesin / Ozbek, Ugur / Telci, Dilek / Sahin, Fikrettin / Yalcin, Koray / Ratip, Siret / Ince, Umit / Ovali, Ercument

Scientific reports

2021 Volume 11, Issue 1, Page(s) 15799

Abstract: The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization ...

Abstract	The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. Hence, the novelty of our vaccine candidate (OZG-38.61.3) is that it is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. Efficiency and safety dose (either 10
MeSH term(s)	Angiotensin-Converting Enzyme 2/genetics ; Angiotensin-Converting Enzyme 2/metabolism ; Animals ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; COVID-19/prevention & control ; COVID-19 Vaccines/immunology ; Chlorocebus aethiops ; Cytokines/metabolism ; Dose-Response Relationship, Immunologic ; Gamma Rays ; Humans ; Immunity ; Lung/pathology ; Mice ; Mice, Inbred BALB C ; Mice, Transgenic ; RNA, Viral ; SARS-CoV-2/immunology ; SARS-CoV-2/radiation effects ; Vaccination ; Vaccines, Inactivated/immunology ; Vero Cells ; Virus Replication
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Cytokines ; RNA, Viral ; Vaccines, Inactivated ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
Language	English
Publishing date	2021-08-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-95086-4
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Article ; Online: Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates.

Sir Karakus, Gozde / Tastan, Cihan / Dilek Kancagi, Derya / Yurtsever, Bulut / Tumentemur, Gamze / Demir, Sevda / Turan, Raife Dilek / Abanuz, Selen / Cakirsoy, Didem / Seyis, Utku / Ozer, Samed / Elibol, Omer / Elek, Muhammer / Ertop, Gurcan / Arbak, Serap / Acikel Elmas, Merve / Hemsinlioglu, Cansu / Kocagoz, Ayse Sesin / Hatirnaz Ng, Ozden /

Akyoney, Sezer / Sahin, Ilayda / Ozbek, Ugur / Telci, Dilek / Sahin, Fikrettin / Yalcin, Koray / Ratip, Siret / Ovali, Ercument

Scientific reports

2021 Volume 11, Issue 1, Page(s) 5804

Abstract: COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo ... ...

Abstract	COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. The candidate vaccines in this study were OZG-3861 version 1 (V1), an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1), a GM-CSF adjuvant added vaccine. The candidate vaccines were applied intradermally to BALB/c mice to assess toxicity and immunogenicity. Preliminary results in vaccinated mice are reported in this study. Especially, the vaccine models containing GM-CSF caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature, when considered in terms of T and B cell responses. Another important finding was that the presence of adjuvant was more important in T cell in comparison with B cell response. Vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study shows that the vaccines are effective and leads us to start the challenge test to investigate the gamma-irradiated inactivated vaccine candidates for infective SARS-CoV-2 virus in humanized ACE2 + mice.
MeSH term(s)	Animals ; COVID-19 Vaccines/immunology ; COVID-19 Vaccines/toxicity ; Female ; Gamma Rays ; Genome, Viral ; Humans ; Immunogenicity, Vaccine ; Male ; Mice, Inbred BALB C ; SARS-CoV-2/genetics ; Vaccines, Inactivated/immunology ; Vaccines, Inactivated/toxicity ; Mice
Chemical Substances	COVID-19 Vaccines ; Vaccines, Inactivated
Language	English
Publishing date	2021-03-11
Publishing country	England
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-83930-6
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Article ; Online: Preclinical efficacy and safety analysis of gamma-irradiated inactivated SARS-CoV-2 vaccine candidates

Gozde Sir Karakus / Cihan Tastan / Derya Dilek Kancagi / Bulut Yurtsever / Gamze Tumentemur / Sevda Demir / Raife Dilek Turan / Selen Abanuz / Didem Cakirsoy / Utku Seyis / Samed Ozer / Omer Elibol / Muhammer Elek / Gurcan Ertop / Serap Arbak / Merve Acikel Elmas / Cansu Hemsinlioglu / Ayse Sesin Kocagoz / Ozden Hatirnaz Ng /

Sezer Akyoney / Ilayda Sahin / Ugur Ozbek / Dilek Telci / Fikrettin Sahin / Koray Yalcin / Siret Ratip / Ercument Ovali

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 19

Abstract: Abstract COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in ...

Abstract	Abstract COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, SARS-CoV-2 vaccines have become crucial for reducing morbidity and mortality. In this study, in vitro and in vivo safety and efficacy analyzes of lyophilized vaccine candidates inactivated by gamma-irradiation were performed. The candidate vaccines in this study were OZG-3861 version 1 (V1), an inactivated SARS-CoV-2 virus vaccine, and SK-01 version 1 (V1), a GM-CSF adjuvant added vaccine. The candidate vaccines were applied intradermally to BALB/c mice to assess toxicity and immunogenicity. Preliminary results in vaccinated mice are reported in this study. Especially, the vaccine models containing GM-CSF caused significant antibody production with neutralization capacity in absence of the antibody-dependent enhancement feature, when considered in terms of T and B cell responses. Another important finding was that the presence of adjuvant was more important in T cell in comparison with B cell response. Vaccinated mice showed T cell response upon restimulation with whole inactivated SARS-CoV-2 or peptide pool. This study shows that the vaccines are effective and leads us to start the challenge test to investigate the gamma-irradiated inactivated vaccine candidates for infective SARS-CoV-2 virus in humanized ACE2 + mice.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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