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  1. Article: Cutaneous leishmaniasis lowers the quality of life: a neglected truth.

    Aghakhani, Nader / Azami, Mehdi / Mohaghegh, Mohammad Ali

    GMS hygiene and infection control

    2023  Volume 18, Page(s) Doc21

    Language English
    Publishing date 2023-09-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2717852-3
    ISSN 2196-5226
    ISSN 2196-5226
    DOI 10.3205/dgkh000447
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Cutaneous leishmaniasis lowers the quality of life: a neglected truth

    Aghakhani, Nader / Azami, Mehdi / Mohaghegh, Mohammad Ali

    GMS Hygiene and Infection Control

    2023  Volume 18, Page(s) 21

    Title translation Kutane Leishmaniose senkt die Lebensqualität: eine vernachlässigte Wahrheit
    Keywords Medizin, Gesundheit
    Publishing date 2023-09-21
    Publisher German Medical Science GMS Publishing House; Düsseldorf
    Document type Article ; Online
    ZDB-ID 2717852-3
    ISSN 2196-5226 ; 2196-5226
    ISSN (online) 2196-5226
    ISSN 2196-5226
    DOI 10.3205/dgkh000447
    Database German Medical Science

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  3. Article ; Online: Cutaneous leishmaniasis lowers the quality of life

    Aghakhani, Nader / Azami, Mehdi / Mohaghegh, Mohammad Ali

    GMS Hygiene and Infection Control, Vol 18, p Doc

    a neglected truth

    2023  Volume 21

    Keywords Medicine ; R ; Public aspects of medicine ; RA1-1270 ; Microbiology ; QR1-502
    Language German
    Publishing date 2023-09-01T00:00:00Z
    Publisher German Medical Science GMS Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Identification and differentiation of Fasciola hepatica and F. gigantica using multiplex PCR technique.

    Heydarian, Peyman / Mamaghani, Amirreza Javadi / Hajialilo, Elham / Bozorgomid, Arezoo / Mohaghegh, Mohammad Ali / Aryaeipour, Mojgan / Afshar, Mohammad Javad Abbaszadeh / Jajarmi, Vahid

    Annals of parasitology

    2023  Volume 69, Issue 2, Page(s) 67–74

    Abstract: We aimed to present an alternate method instead of PCR-RFLP and also develop an optimized method for rapid, time-saving and affordable molecular-based approach to discriminate species of liver fluke, Fasciola hepatica and F. gigantica. Seventy-six ... ...

    Abstract We aimed to present an alternate method instead of PCR-RFLP and also develop an optimized method for rapid, time-saving and affordable molecular-based approach to discriminate species of liver fluke, Fasciola hepatica and F. gigantica. Seventy-six samples of F. hepatica and 28 F. gigantica were collected from the slaughterhouses of endemic regions in Iran. Following a comprehensive analysis of the mitochondrial complete sequences of both F. hepatica and F. gigantica, the extracted DNAs from all samples were used as templates in multiplex PCR reactions containing two sets of primers specific for cytochrome c oxidase I (cox I) gene of both species. In a parallel experiment, PCR-RFLP was performed for each sample using internal transcribed spacer (ITS1) sequence. Furthermore, following a PCR amplification for cox I gene, the amplicons were purified for sequencing. To assess the validity of the multiplex PCR approach, the obtained data from the multiplex PCR and PCR-RFLP experiments were compared with each other. By sequence analysis of 104 samples, 76 and 28 samples were identified as F. hepatica and F. gigantica, respectively. Results revealed 100% and 92% of accuracy as for multiplex PCR and PCR-RFLP. The designed multiplex PCR strategy offers a valid alternative approach to the conventional methods with distinctive features including convenience, cost-effectiveness, time-saving (3 hours from sampling to obtain final results) and high efficacy.
    MeSH term(s) Animals ; Fasciola hepatica/genetics ; Fasciola/genetics ; Fascioliasis/diagnosis ; Fascioliasis/epidemiology ; Fascioliasis/veterinary ; Multiplex Polymerase Chain Reaction ; DNA, Ribosomal Spacer/genetics
    Chemical Substances DNA, Ribosomal Spacer
    Language English
    Publishing date 2023-12-30
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2672322-0
    ISSN 2299-0631 ; 0043-5163
    ISSN 2299-0631 ; 0043-5163
    DOI 10.17420/ap6902.511
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 102: Show issues Location:
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  5. Article ; Online: Evaluation of scolicidal potential of salicylate coated zinc nanoparticles against Echinococcus granulosus protoscoleces.

    Cheraghipour, Kourosh / Azarhazine, Mohammad / Zivdari, Masoomeh / Beiranvand, Marjan / Shakib, Pegah / Rashidipour, Marzieh / Mardanshah, Omid / Mohaghegh, Mohammad Ali / Marzban, Abdolrazagh

    Experimental parasitology

    2023  Volume 246, Page(s) 108456

    Abstract: Echinococcosis is a zoonotic disease caused by larval stages of the Echinococcus genus (metastasis). In this study, salicylate-coated Zinc oxide nanoparticles (SA-ZnO-NPs) were fabricated and characterized by SEM, FTIR and XRD analytical techniques. ... ...

    Abstract Echinococcosis is a zoonotic disease caused by larval stages of the Echinococcus genus (metastasis). In this study, salicylate-coated Zinc oxide nanoparticles (SA-ZnO-NPs) were fabricated and characterized by SEM, FTIR and XRD analytical techniques. After that, different doses of SA-ZnO-NPs, SA and ZnO-NPs were taken to assess scolicidal potency. Scanning electron microscopy (SEM) micrographs were also used to evaluate the morphological deformities of treated protoscoleces. Furthermore, Caspase-3&7 inductions were examined in protoscoleces cysts treated with all formulations. Based on SEM and DLS analyses, the size of SA-ZnO-NPs was between 30 and 40 nm, with a spherical shape. The FTIR spectrum verified the presence of SA functional groups on the ZnO coating. At 20 min, SA-ZnO-NPs at 2000 μg/ml exhibited the greatest activity on protoscolices with 100% mortality, followed by ZnO-NPs at 1500 μg/ml at 10 min and SA alone at 2000 μg/ml at 30 min. The activation of Caspase-3&7 apoptotic enzyme was determined for 2000 μg/ml of SA-ZnO-NPs, ZnO-NPs and SA to be 16.4, 31.4, and 35.7%, respectively. The SEM image revealed apoptogenic alterations and the induction of tegument surface wrinkles, as well as abnormalities in rostellum protoscolices. According to the current study, SA-ZnO-NPs have a high mortality rate against hydatid cyst protoscolices. As a result, further studies on the qualitative assessment of these nanoformulations in vivo and preclinical animal trials seem to be required. Furthermore, the adoption of nano-drugs potentially offers alternative therapeutic approaches to combat hydatid cysts.
    MeSH term(s) Animals ; Echinococcus granulosus ; Caspase 3 ; Zinc ; Zinc Oxide/pharmacology ; Metal Nanoparticles/therapeutic use ; Salicylates/pharmacology ; Salicylates/therapeutic use ; Echinococcosis/drug therapy ; Echinococcus ; Nanoparticles
    Chemical Substances Caspase 3 (EC 3.4.22.-) ; Zinc (J41CSQ7QDS) ; Zinc Oxide (SOI2LOH54Z) ; Salicylates
    Language English
    Publishing date 2023-01-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391089-1
    ISSN 1090-2449 ; 0014-4894
    ISSN (online) 1090-2449
    ISSN 0014-4894
    DOI 10.1016/j.exppara.2022.108456
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Data on the prevalence and distribution of carbapenemase genes in Enterobacterales species isolated from clinical specimens in the center of Irans.

    Badmasti, Farzad / Azizi, Omid / Mohaghegh, Mohammad Ali / Solgi, Hamid

    Data in brief

    2021  Volume 38, Page(s) 107368

    Abstract: Carbapenem resistance in Enterobacterales is a major and persistent public health problem worldwide. In current research, we present data of 96 Enterobacterales species collected from a clinical hospital in Isfahan, Iran. The bacterial identification was ...

    Abstract Carbapenem resistance in Enterobacterales is a major and persistent public health problem worldwide. In current research, we present data of 96 Enterobacterales species collected from a clinical hospital in Isfahan, Iran. The bacterial identification was performed by standard biochemical tests and API 20E methods. Agar disk diffusion assay was performed to determine the phenotypic antibiotic resistance of strains. Polymerase chain reaction (PCR) was carried out to detect carbapenemase genes. In this manuscript, multiple antimicrobial resistance phenotype such as multiple carbapenem resistance determinants were detected. The data would provide important information on distribution of carbapenemase genes of those pathogenic bacteria in Iran.
    Language English
    Publishing date 2021-09-13
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2786545-9
    ISSN 2352-3409 ; 2352-3409
    ISSN (online) 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2021.107368
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  7. Article: High contamination of soil with Toxocara spp. eggs in the north of Iran.

    Mohaghegh, Mohammad Ali / Rezaiemanesh, Mohammad Reza / Resketi, Mojtaba Azimi / Ghomashlooyan, Mohsen / Falahati, Mohammad / Cheraghipour, Kourosh / Peyman, Marziyeh / Mazhab-Jafari, Komeil

    Annals of parasitology

    2022  Volume 67, Issue 4, Page(s) 715–721

    Abstract: Toxocarosis is an important emerging zoonotic disease, which is caused by larvae of Toxocara canis and Toxocara cati, the most widely distributed nematode parasites of dogs and cats. The soil, where Toxocara eggs become infective, is the natural source ... ...

    Abstract Toxocarosis is an important emerging zoonotic disease, which is caused by larvae of Toxocara canis and Toxocara cati, the most widely distributed nematode parasites of dogs and cats. The soil, where Toxocara eggs become infective, is the natural source of infection. The aim of this work was to determine the levels of soil contamination with Toxocara spp. eggs in Sari district, north of Iran, in order to estimate the risk of infection of the population living in this region. A total of 141 soil samples were collected from 12 parks, 12 primary schools and 45 backyards from three regions. Soil samples were investigated for the presence of Toxocara eggs by flotation technique using sucrose solution and examined under light microscope using 10× and 40× objective. Out of 141 soil samples, 67 (47.5%) were found to contain Toxocara spp. eggs. The contamination rate in public parks, primary schools and backyards were observed 50%, 58.3% and 33.3%, respectively. The comparison of soil contamination rate from different areas, public parks, primary schools and backyards, was statistically significant (χ2=6.00, d.f.=2 and P=0.049). The public parks, backyards and primary schools, which are main places that children play, are suitable for defecation of dogs and cats. The knowledge of the soil contamination with Toxocara eggs indicates the need to take an appropriate and effective measures to prevent the infection.
    MeSH term(s) Animals ; Cat Diseases ; Cats ; Dog Diseases ; Dogs ; Iran/epidemiology ; Soil/parasitology ; Toxocara
    Chemical Substances Soil
    Language English
    Publishing date 2022-03-15
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2672322-0
    ISSN 2299-0631 ; 0043-5163
    ISSN 2299-0631 ; 0043-5163
    DOI 10.17420/ap6704.388
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 102: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  8. Article: Genetic diversity of

    Khoshnood, Saleh / Tavalla, Mehdi / Mohaghegh, Mohammad Ali / Khamesipour, Faham / Hejazi, Seyed Hossein

    Journal of parasitic diseases : official organ of the Indian Society for Parasitology

    2021  Volume 45, Issue 3, Page(s) 838–844

    Abstract: This study aimed to screen the natural infection rate ... ...

    Abstract This study aimed to screen the natural infection rate of
    Language English
    Publishing date 2021-03-10
    Publishing country India
    Document type Journal Article
    ZDB-ID 2548456-4
    ISSN 0975-0703 ; 0971-7196
    ISSN (online) 0975-0703
    ISSN 0971-7196
    DOI 10.1007/s12639-021-01369-y
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  9. Article ; Online: Data on the prevalence and distribution of carbapenemase genes in Enterobacterales species isolated from clinical specimens in the center of Irans

    Farzad Badmasti / Omid Azizi / Mohammad Ali Mohaghegh / Hamid Solgi

    Data in Brief, Vol 38, Iss , Pp 107368- (2021)

    2021  

    Abstract: Carbapenem resistance in Enterobacterales is a major and persistent public health problem worldwide. In current research, we present data of 96 Enterobacterales species collected from a clinical hospital in Isfahan, Iran. The bacterial identification was ...

    Abstract Carbapenem resistance in Enterobacterales is a major and persistent public health problem worldwide. In current research, we present data of 96 Enterobacterales species collected from a clinical hospital in Isfahan, Iran. The bacterial identification was performed by standard biochemical tests and API 20E methods. Agar disk diffusion assay was performed to determine the phenotypic antibiotic resistance of strains. Polymerase chain reaction (PCR) was carried out to detect carbapenemase genes. In this manuscript, multiple antimicrobial resistance phenotype such as multiple carbapenem resistance determinants were detected. The data would provide important information on distribution of carbapenemase genes of those pathogenic bacteria in Iran.
    Keywords Enterobacterales species ; Antibiotic resistance ; Carbapenemase genes ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Echinococcus granulosus sheep strain (G1) as the predominant genotype in definitive host (dogs) isolates in northeastern Iran.

    Hejazi, Seyed-Hossein / Mirbadie, Seyed-Reza / Jafari, Rasool / Rezaiemanesh, Mohammad-Reza / Azizi, Omid / Badmasti, Farzad / Kalani, Hamed / Cheraghipour, Kourosh / Heydarian, Peyman / Hashemi, Nooshin / Izadi, Shahrokh / Jabalameli, Zahra / Mohaghegh, Mohammad-Ali

    Veterinary parasitology, regional studies and reports

    2023  Volume 48, Page(s) 100975

    Abstract: Echinococcus granulosus sensu lato (E. granulosus s.l.) is a zoonotic parasite, causing cystic echinococcosis in humans. In the present study, prevalence and genotypes of E. granulosus s.l. was assessed in stools collected from 244 dogs including 138 ... ...

    Abstract Echinococcus granulosus sensu lato (E. granulosus s.l.) is a zoonotic parasite, causing cystic echinococcosis in humans. In the present study, prevalence and genotypes of E. granulosus s.l. was assessed in stools collected from 244 dogs including 138 stray and 106 domestic animals using high resolution melting curve (HRM) method. Initially, to detect taeniid eggs in feces, all samples were examined using the formalin-ether techniques. Genomic DNA was extracted from the positive samples and E. granulosus s.l. was differentiated from other Taeniidae parasites using SSU-rDNA gene and E. granulosus s.l. was analyzed for genotyping using HRM based on the cox1 gene. In total, 12.7% (31/244) of the samples were positive for Taeniidae eggs. In addition, among the positive samples, 77.4% (24/31) were positive for E. granulosus s.l.. In details, 11.3% (12/106) of the domestic dogs and 8.7% (12/138) of the stray dogs were positive for E. granulosus s.l.. The results of HRM analysis showed that all E. granulosus s.l. isolates were G1 strain. Findings of the present study indicated a considerable prevalence of E. granulosus G1 among dogs in the northeast of Iran and imply a serious risk of transmitting to humans and livestock.
    MeSH term(s) Sheep ; Dogs ; Animals ; Humans ; Echinococcus granulosus/genetics ; Iran/epidemiology ; Echinococcosis/epidemiology ; Echinococcosis/veterinary ; Echinococcosis/diagnosis ; Genotype ; Polymerase Chain Reaction/veterinary ; Dog Diseases/parasitology ; Sheep Diseases
    Language English
    Publishing date 2023-12-17
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2405-9390
    ISSN (online) 2405-9390
    DOI 10.1016/j.vprsr.2023.100975
    Database MEDical Literature Analysis and Retrieval System OnLINE

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