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  1. Article: Improving Biocompatibility of Polyurethanes Apply in Medicine Using Oxygen Plasma and Its Negative Effect on Increased Bacterial Adhesion.

    Drożdż, Kamil / Gołda-Cępa, Monika / Chytrosz-Wróbel, Paulina / Kotarba, Andrzej / Brzychczy-Włoch, Monika

    International journal of biomaterials

    2024  Volume 2024, Page(s) 5102603

    Abstract: Polyurethanes (PUs) are versatile polymers used in medical applications due to their high flexibility and fatigue resistance. PUs are widely used for synthetic blood vessels, wound dressings, cannulas, and urinary and cardiovascular catheters. Many ... ...

    Abstract Polyurethanes (PUs) are versatile polymers used in medical applications due to their high flexibility and fatigue resistance. PUs are widely used for synthetic blood vessels, wound dressings, cannulas, and urinary and cardiovascular catheters. Many scientific reports indicate that surface wettability is crucial for biocompatibility and bacterial adhesion. The use of oxygen plasma to modify PUs is advantageous because of its effectiveness in introducing oxygen-containing functional groups, thereby altering surface wettability. The purpose of this study was to investigate the effect of the modification of the oxygen plasma of polyurethane on its biocompatibility with lung tissue (A549 cell line) and the adhesion of Gram-positive bacteria (
    Language English
    Publishing date 2024-02-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2494344-7
    ISSN 1687-8795 ; 1687-8787
    ISSN (online) 1687-8795
    ISSN 1687-8787
    DOI 10.1155/2024/5102603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Immunogenic Proteins of Group B Streptococcus-Potential Antigens in Immunodiagnostic Assay for GBS Detection.

    Dobrut, Anna / Brzychczy-Włoch, Monika

    Pathogens (Basel, Switzerland)

    2021  Volume 11, Issue 1

    Abstract: Streptococcus ... ...

    Abstract Streptococcus agalactiae
    Language English
    Publishing date 2021-12-31
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11010043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Immunogenic Proteins of Group B Streptococcus—Potential Antigens in Immunodiagnostic Assay for GBS Detection

    Anna Dobrut / Monika Brzychczy-Włoch

    Pathogens, Vol 11, Iss 43, p

    2022  Volume 43

    Abstract: Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen, which asymptomatically colonizes the gastrointestinal and genitourinary tract of up to one third of healthy adults. Nevertheless, GBS carriage in pregnant women may lead ... ...

    Abstract Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen, which asymptomatically colonizes the gastrointestinal and genitourinary tract of up to one third of healthy adults. Nevertheless, GBS carriage in pregnant women may lead to several health issues in newborns causing life threatening infection, such as sepsis, pneumonia or meningitis. Recommended GBS screening in pregnant women significantly reduced morbidity and mortality in infants. Nevertheless, intrapartum antibiotic prophylaxis, recommended following the detection of carriage or in case of lack of a carriage test result for pregnant women who demonstrate certain risk factors, led to the expansion of the adverse phenomenon of bacterial resistance to antibiotics. In our paper, we reviewed some immunogenic GBS proteins, i.e., Alp family proteins, β protein, Lmb, Sip, BibA, FsbA, ScpB, enolase, elongation factor Tu, IMPDH, and GroEL, which possess features characteristic of good candidates for immunodiagnostic assays for GBS carriage detection, such as immunoreactivity and specificity. We assume that they can be used as an alternative diagnostic method to the presently recommended bacteriological cultivation and MALDI.
    Keywords Streptococcus agalactiae ; Group B Streptococcus ; immunogenic proteins ; immunodiagnostics ; proteomics ; newborn infections ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Stethoscopes or Maybe "Bacterioscopes" - Is hand Hygiene Solely Capable of Preventing Hospital-Associated Infections?

    Talaga-Ćwiertnia, Katarzyna / Ochońska, Dorota / Gajda, Mateusz / Kowalczyk, Monika / Palczewska, Magdalena / Brzychczy-Włoch, Monika

    Polish journal of microbiology

    2023  Volume 72, Issue 1, Page(s) 79–91

    Abstract: The stethoscope remains an indispensable diagnostic tool for medical students. Improper stethoscope hygiene may cause bacterial infections, including hospital-associated infections (HAIs), which challenge the Polish medical system. The study's main ... ...

    Abstract The stethoscope remains an indispensable diagnostic tool for medical students. Improper stethoscope hygiene may cause bacterial infections, including hospital-associated infections (HAIs), which challenge the Polish medical system. The study's main objective was to evaluate the hygiene habits declared by medical students. Moreover, microbiological control with the characteristics of potentially pathogenic microorganisms was performed. The study included 66 medical students from the Faculty of Medicine at the Jagiellonian University Medical College in Cracow, Poland. The participants filled in an anonymous questionnaire. Stethoscope contamination was assessed through isolation, identification, testing of antibiotic resistance, and clonality of the isolates bacterial pathogens. The survey showed that only 30.3% of students cleaned their stethoscopes after each patient, and 1.5% never did this. Of the 66 stethoscopes tested, 100% were positive for bacterial growth.
    MeSH term(s) Humans ; Stethoscopes/microbiology ; Hand Hygiene ; Cross Infection/prevention & control ; Cross Infection/microbiology ; Bacteria ; Hospitals ; Equipment Contamination/prevention & control
    Language English
    Publishing date 2023-03-24
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2234080-4
    ISSN 2544-4646 ; 1733-1331
    ISSN (online) 2544-4646
    ISSN 1733-1331
    DOI 10.33073/pjm-2023-012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Do NAAT-Based Methods Increase the Diagnostic Sensitivity of

    Sroka-Oleksiak, Agnieszka / Pabian, Wojciech / Sobońska, Joanna / Drożdż, Kamil / Bogiel, Tomasz / Brzychczy-Włoch, Monika

    Diagnostics (Basel, Switzerland)

    2023  Volume 13, Issue 5

    Abstract: The aim of the study was to evaluate particular polymerase chain reaction primers targeting selected representative genes and the influence of a preincubation step in a selective broth on the sensitivity of group ... ...

    Abstract The aim of the study was to evaluate particular polymerase chain reaction primers targeting selected representative genes and the influence of a preincubation step in a selective broth on the sensitivity of group B
    Language English
    Publishing date 2023-02-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics13050863
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Biofilm Formation of Clinical

    Ochońska, Dorota / Ścibik, Łukasz / Brzychczy-Włoch, Monika

    Pathogens (Basel, Switzerland)

    2021  Volume 10, Issue 10

    Abstract: 1) Background: Due to the commonness of tracheotomy procedures and the wide use of biomaterials in the form of tracheostomy tubes (TTs), the problem of biomaterial-associated infections (BAIs) is growing. Bacterial colonization of TTs results in the ... ...

    Abstract (1) Background: Due to the commonness of tracheotomy procedures and the wide use of biomaterials in the form of tracheostomy tubes (TTs), the problem of biomaterial-associated infections (BAIs) is growing. Bacterial colonization of TTs results in the development of biofilms on the surface of biomaterials, which may contribute to the development of invasive infections in tracheostomized patients. (2) Methods: Clinical strains of
    Language English
    Publishing date 2021-10-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens10101345
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  7. Article: The Frequency of Occurrence of Resistance and Genes Involved in the Process of Adhesion and Accumulation of Biofilm in

    Drożdż, Kamil / Ochońska, Dorota / Ścibik, Łukasz / Gołda-Cępa, Monika / Biegun, Katarzyna / Brzychczy-Włoch, Monika

    Microorganisms

    2022  Volume 10, Issue 6

    Abstract: ... ...

    Abstract Background
    Language English
    Publishing date 2022-06-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10061210
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Sonochemical Deposition of Gentamicin Nanoparticles at the PCV Tracheostomy Tube Surface Limiting Bacterial Biofilm Formation.

    Ścibik, Łukasz / Ochońska, Dorota / Gołda-Cępa, Monika / Kwiecień, Konrad / Pamuła, Elżbieta / Kotarba, Andrzej / Brzychczy-Włoch, Monika

    Materials (Basel, Switzerland)

    2023  Volume 16, Issue 10

    Abstract: Background: The use of nanotechnology in the production of medical equipment has opened new possibilities to fight bacterial biofilm developing on their surfaces, which can cause infectious complications. In this study, we decided to use gentamicin ... ...

    Abstract Background: The use of nanotechnology in the production of medical equipment has opened new possibilities to fight bacterial biofilm developing on their surfaces, which can cause infectious complications. In this study, we decided to use gentamicin nanoparticles. An ultrasonic technique was used for their synthesis and immediate deposition onto the surface of tracheostomy tubes, and their effect on bacterial biofilm formation was evaluated.
    Methods: Polyvinyl chloride was functionalized using oxygen plasma followed by sonochemical formation and the embedment of gentamicin nanoparticles. The resulting surfaces were characterized with the use of AFM, WCA, NTA, FTIR and evaluated for cytotoxicity with the use of A549 cell line and for bacterial adhesion using reference strains of
    Results: The use of gentamicin nanoparticles significantly reduced the adhesion of bacterial colonies on the surface of the tracheostomy tube for
    Conclusions: The use of gentamicin nanoparticles on the polyvinyl chloride surface may be an additional supporting method for patients after tracheostomy in order to prevent the colonization of the biomaterial by potentially pathogenic microorganisms.
    Language English
    Publishing date 2023-05-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma16103765
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Standardization of the protocol for oral cavity examination and collecting of the biological samples for microbiome research using the next-generation sequencing (NGS): own experience with the COVID-19 patients.

    Brzychczy-Sroka, Barbara / Talaga-Ćwiertnia, Katarzyna / Sroka-Oleksiak, Agnieszka / Gurgul, Artur / Zarzecka-Francica, Elżbieta / Ostrowski, Wojciech / Kąkol, Janusz / Drożdż, Kamil / Brzychczy-Włoch, Monika / Zarzecka, Joanna

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3717

    Abstract: To date, publications have shown that compositions of oral microbiota differ depending on their habitats (e.g. tongue, tonsils, pharynx). The absence of set standards for the choice of the areas and conditions of material collection makes the oral ... ...

    Abstract To date, publications have shown that compositions of oral microbiota differ depending on their habitats (e.g. tongue, tonsils, pharynx). The absence of set standards for the choice of the areas and conditions of material collection makes the oral microbiome one of the most difficult environments for a comparative analysis with other researchers, which is a meaningful limitation during an assessment of the potential effects of microorganisms as biomarkers in the courses of various human diseases. Therefore, standardisation of basic conditions of a dental examination and collection of material for the next generation sequencing (NGS) is worth attempting. The standardisation of the dental exam and collection of the clinical materials: saliva, swab from the tongue ridge, hard palate, palatine tonsils and oropharynx, supragingival plaque and subgingival plaque. Protocol involved the patients (n = 60), assigned to 3 groups: I-COVID-19 convalescents who received antibiotics, n = 17, II-COVID-19 convalescents, n = 23 and III-healthy individuals, n = 20. The collected biological samples were used to conduct NGS (16S rRNA). The conditions of patient preparation for collecting biological materials as well as the schedule of dental examination, were proposed. Based on the research conducted, we have indicated the dental indicators that best differentiate the group of COVID-19 patients (groups I and II) from healthy people (group III). These include the DMFT, D and BOP indices. The use of alpha and beta diversity analysis provided an overall insight into the diversity of microbial communities between specific niches and patient groups. The most different diversity between the studied group of patients (group II) and healthy people (group III) was noted in relation to the supragingival plaque. The order of activities during the dental exam as well as while collecting and securing clinical materials is particularly important to avoid technical errors and material contamination which may result in erroneous conclusions from the analyses of the results of sensitive tests such as the NGS. It has been shown that the dental indices: DMFT, D number, PI and BOP are the best prognostic parameters to assess the oral health. Based on beta diversity the most sensitive niche and susceptible to changes in the composition of the microbiota is the supragingival plaque. The procedures developed by our team can be applied as ready-to-use forms in studies conducted by other researchers.
    MeSH term(s) Humans ; RNA, Ribosomal, 16S/genetics ; COVID-19/diagnosis ; Microbiota/genetics ; Mouth ; High-Throughput Nucleotide Sequencing ; Reference Standards
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-02-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-53992-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Immunogenic Proteins of Group B Streptococcus—Potential Antigens in Immunodiagnostic Assay for GBS Detection

    Dobrut, Anna / Brzychczy-Włoch, Monika

    Pathogens. 2021 Dec. 31, v. 11, no. 1

    2021  

    Abstract: Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen, which asymptomatically colonizes the gastrointestinal and genitourinary tract of up to one third of healthy adults. Nevertheless, GBS carriage in pregnant women may lead ... ...

    Abstract Streptococcus agalactiae (Group B Streptococcus, GBS) is an opportunistic pathogen, which asymptomatically colonizes the gastrointestinal and genitourinary tract of up to one third of healthy adults. Nevertheless, GBS carriage in pregnant women may lead to several health issues in newborns causing life threatening infection, such as sepsis, pneumonia or meningitis. Recommended GBS screening in pregnant women significantly reduced morbidity and mortality in infants. Nevertheless, intrapartum antibiotic prophylaxis, recommended following the detection of carriage or in case of lack of a carriage test result for pregnant women who demonstrate certain risk factors, led to the expansion of the adverse phenomenon of bacterial resistance to antibiotics. In our paper, we reviewed some immunogenic GBS proteins, i.e., Alp family proteins, β protein, Lmb, Sip, BibA, FsbA, ScpB, enolase, elongation factor Tu, IMPDH, and GroEL, which possess features characteristic of good candidates for immunodiagnostic assays for GBS carriage detection, such as immunoreactivity and specificity. We assume that they can be used as an alternative diagnostic method to the presently recommended bacteriological cultivation and MALDI.
    Keywords Streptococcus agalactiae ; antibiotics ; diagnostic techniques ; disease prevention ; gastrointestinal system ; immune response ; meningitis ; morbidity ; mortality ; opportunistic pathogens ; peptide elongation factors ; phosphopyruvate hydratase ; pneumonia
    Language English
    Dates of publication 2021-1231
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11010043
    Database NAL-Catalogue (AGRICOLA)

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