Article ; Online: Expression of cagA, virB/D Complex and/or vacA Genes in Helicobacter pylori Strains Originating from Patients with Gastric Diseases.
PloS one
2016 Volume 11, Issue 2, Page(s) e0148936
Abstract: ... of its carcinogenic activity, we analysed expression of virulence genes: cagA, virB/D complex (virB4, virB7, virB8 ... in all strains of H. pylori cagA+ (subgroup 1) high expression of the gene as well as of virB/D was disclosed ... strains). In both groups high expression of virB/D and vacA was disclosed. Our results indicate ...
| Abstract | In order to better understand pathogenicity of Helicobacter pylori, particularly in the context of its carcinogenic activity, we analysed expression of virulence genes: cagA, virB/D complex (virB4, virB7, virB8, virB9, virB10, virB11, virD4) and vacA in strains of the pathogen originating from persons with gastric diseases. The studies were conducted on 42 strains of H. pylori isolated from patients with histological diagnosis of non-atrophic gastritis-NAG (group 1, including subgroup 1 containing cagA+ isolates and subgroup 2 containing cagA- strains), multifocal atrophic gastritis-MAG (group 2) and gastric adenocarcinoma-GC (group 3). Expression of H. pylori genes was studied using microarray technology. In group 1, in all strains of H. pylori cagA+ (subgroup 1) high expression of the gene as well as of virB/D was disclosed, accompanied by moderate expression of vacA. In strains of subgroup 2 a moderate expression of vacA was detected. All strains in groups 2 and 3 carried cagA gene but they differed in its expression: a high expression was detected in isolates of group 2 and its hyperexpression in strains of group 3 (hypervirulent strains). In both groups high expression of virB/D and vacA was disclosed. Our results indicate that chronic active gastritis may be induced by both cagA+ strains of H. pylori, manifesting high expression of virB/D complex but moderate activity of vacA, and cagA- strains with moderate expression of vacA gene. On the other hand, in progression of gastric pathology and carcinogenesis linked to H. pylori a significant role was played by hypervirulent strains, manifesting a very high expression of cagA and high activity of virB/D and vacA genes. |
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| MeSH term(s) | Adult ; Aged ; Antigens, Bacterial/biosynthesis ; Antigens, Bacterial/genetics ; Antigens, Bacterial/metabolism ; Bacterial Outer Membrane Proteins/biosynthesis ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Bacterial Proteins/biosynthesis ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Exons ; Female ; Gastritis, Atrophic/microbiology ; Gastroscopy ; Gene Expression Regulation, Bacterial ; Helicobacter Infections/genetics ; Helicobacter Infections/metabolism ; Helicobacter Infections/microbiology ; Helicobacter pylori/genetics ; Humans ; Male ; Middle Aged ; Oligonucleotide Array Sequence Analysis ; Real-Time Polymerase Chain Reaction ; Stomach Diseases/microbiology ; Stomach Neoplasms/microbiology |
| Chemical Substances | Antigens, Bacterial ; Bacterial Outer Membrane Proteins ; Bacterial Proteins ; VacA protein, Helicobacter pylori ; cagA protein, Helicobacter pylori |
| Language | English |
| Publishing date | 2016-02-11 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0148936 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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