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Article: Endogenous Retroviral-K Envelope Is a Novel Tumor Antigen and Prognostic Indicator of Renal Cell Carcinoma.

Weyerer, Veronika / Strissel, Pamela L / Stöhr, Christine / Eckstein, Markus / Wach, Sven / Taubert, Helge / Brandl, Lisa / Geppert, Carol I / Wullich, Bernd / Cynis, Holger / Beckmann, Matthias W / Seliger, Barbara / Hartmann, Arndt / Strick, Reiner

Frontiers in oncology

2021 Volume 11, Page(s) 657187

Abstract: ... targets for cancer (immune) therapy. ERV-K env is overexpressed and implicated as a therapeutic target ... with an ERV-K env antibody and correlated with patient clinical data. TMA results showed the highest amount ... of ERV-K env protein expression and the strongest significant membrane expression in ccRCC versus ...

Abstract	Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. Although epigenetically silenced, endogenous retroviral (ERV) genes become activated in tumors and function to ignite immune responses. Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. ERV-K env is overexpressed and implicated as a therapeutic target for breast cancer, however studies in RCC are limited. In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. TMA results showed the highest amount of ERV-K env protein expression and the strongest significant membrane expression in ccRCC versus other RCC subtypes. High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. Transfecting these cell lines with a codon optimized
Language	English
Publishing date	2021-04-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2021.657187
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Article ; Online: Endogenous Retroviral-K Envelope is a Novel Tumor Antigen and Prognostic Indicator of Renal Cell Carcinoma

Weyerer, Veronika / Strissel, Pamela L. / Stöhr, Christine / Eckstein, Markus / Wach, Sven / Taubert, Helge / Brandl, Lisa / Geppert, Carol I. / Wullich, Bernd / Cynis, Holger / Beckmann, Matthias W. / Seliger, Barbara / Hartmann, Arndt / Strick, Reiner

2021

Abstract: ... and their role as tumor antigens and targets for cancer (immune) therapy. ERV-K env is overexpressed ... histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data ... TMA results showed the highest amount of ERV-K env protein expression and the strongest significant ...

Abstract	Art. 657187, 12 S. Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. Although epigenetically silenced, endogenous retroviral (ERV) genes become activated in tumors and function to ignite immune responses. Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. ERV-K env is overexpressed and implicated as a therapeutic target for breast cancer, however studies in RCC are limited. In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. TMA results showed the highest amount of ERV-K env protein expression and the strongest significant membrane expression in ccRCC versus other RCC subtypes. High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. Transfecting these cell lines with a codon optimized ERV-K113 env overexpressing CMV vector was performed with or without 5'-Aza-2'-deoxycytidine (Aza) treatment to sustain promoter de-methylation. MZ1257RC showed induction of ERV-K113 expression and significantly increased both proliferation and invasion in the presence or absence of Aza. HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. However, in the presence of Aza despite increased ERV-K113 env expression, an inhibition of HEK293 proliferation and a further restriction of invasion was ...
Keywords	human endogenous retrovirus ; ERV-K ; renal cell carcinoma ; tumor antigen ; patient prognosis ; p53 ; Azacytidine ; Invasion ; 610 ; 620
Subject code	616
Language	English
Publishing country	de
Document type	Article ; Online
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Article: Elevated HERV-K Expression in Soft Tissue Sarcoma Is Associated with Worsened Relapse-Free Survival.

Giebler, Maria / Staege, Martin S / Blauschmidt, Sindy / Ohm, Lea I / Kraus, Matthias / Würl, Peter / Taubert, Helge / Greither, Thomas

Frontiers in microbiology

2018 Volume 9, Page(s) 211

Abstract: ... K and HERV-F and the clinicopathological characteristics in a cohort of STSs as well ... as the patients' prognosis was evaluated. HERV-K and HERV-F expression was assessed by quantitative real-time PCR ... in 120 patient specimens. HERV-K and HERV-F expression was significantly correlated ( ...

Abstract	A wide variety of endogenous retroviral sequences has been demonstrated in the human genome so far, divided into several different families according to the sequence homology to viral strains. While increased expression of human endogenous retrovirus (HERV) elements has already been linked to unfavorable prognosis in hepatocellular carcinoma, breast cancer, and ovarian carcinoma yet less is known about the impact of the expression of different HERV elements on sarcomagenesis in general as well as the outcome of soft tissue sarcoma (STS) patients. Therefore, in this study the association between expression of HERV-K and HERV-F and the clinicopathological characteristics in a cohort of STSs as well as the patients' prognosis was evaluated. HERV-K and HERV-F expression was assessed by quantitative real-time PCR in 120 patient specimens. HERV-K and HERV-F expression was significantly correlated (
Language	English
Publishing date	2018-02-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2018.00211
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Article ; Online: Identification of ZNF217, hnRNP-K, VEGF-A and IPO7 as targets for microRNAs that are downregulated in prostate carcinoma.

Szczyrba, Jaroslaw / Nolte, Elke / Hart, Martin / Döll, Celina / Wach, Sven / Taubert, Helge / Keck, Bastian / Kremmer, Elisabeth / Stöhr, Robert / Hartmann, Arndt / Wieland, Wolf / Wullich, Bernd / Grässer, Friedrich A

International journal of cancer

2012 Volume 132, Issue 4, Page(s) 775–784

Abstract: ... heterogeneous nuclear ribonucleoprotein K, hnRNP-K, and vascular endothelial growth factor A, VEGF-A), we identified two regulatory miRNAs ... by their respective miRNAs was confirmed by luciferase assays. As expected, the upregulation of ZNF217, hnRNP-K, VEGF ...

Abstract	In primary prostate cancer (PCa), a major cause of cancer-related death in men, the expression of various microRNAs (miRNAs) is deregulated. We previously detected several miRNAs, for example, miR-24 and miR-22, as significantly downregulated in PCa (Szczyrba et al., Mol Cancer Res 2010;8:529-38). An in silico search predicted that zinc finger protein 217 (ZNF217) and importin 7 (IPO7) were potential target genes of these miRNAs. Additionally, for two genes that are deregulated in PCa (heterogeneous nuclear ribonucleoprotein K, hnRNP-K, and vascular endothelial growth factor A, VEGF-A), we identified two regulatory miRNAs, miR-205 and miR-29b. The regulation of the 3'-untranslated regions of the four genes by their respective miRNAs was confirmed by luciferase assays. As expected, the upregulation of ZNF217, hnRNP-K, VEGF-A and IPO7 could be verified at the protein level in the PCa cell lines LNCaP and DU145. ZNF217 and IPO7, which had not yet been studied in PCa, were analyzed in more detail. ZNF217 mRNA is overexpressed in primary PCa samples, and this overexpression translates to an elevated protein level. However, IPO7 was upregulated at the protein level alone. The inhibition of ZNF217 and IPO7 by siRNA resulted in reduced proliferation of the PCa cell lines. ZNF217 could thus be identified as an oncogene that is overexpressed in PCa and affects the growth of PCa cell lines, whereas the function of IPO7 remains to be elucidated in greater detail.
MeSH term(s)	3' Untranslated Regions ; Aged ; Cell Proliferation ; Down-Regulation ; Gene Expression Regulation, Neoplastic ; Heterogeneous-Nuclear Ribonucleoprotein K/metabolism ; Humans ; Karyopherins/genetics ; Karyopherins/metabolism ; Male ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Middle Aged ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; RNA Interference ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Receptors, Cytoplasmic and Nuclear/genetics ; Receptors, Cytoplasmic and Nuclear/metabolism ; Trans-Activators/genetics ; Trans-Activators/metabolism ; Up-Regulation ; Vascular Endothelial Growth Factor A/metabolism
Chemical Substances	3' Untranslated Regions ; Heterogeneous-Nuclear Ribonucleoprotein K ; IPO7 protein, human ; Karyopherins ; MIRN205 microRNA, human ; MIRN22 microRNA, human ; MIRN24 microRNA, human ; MIRN29a microRNA, human ; MicroRNAs ; RNA, Messenger ; RNA, Small Interfering ; Receptors, Cytoplasmic and Nuclear ; Trans-Activators ; Vascular Endothelial Growth Factor A ; ZNF217 protein, human
Language	English
Publishing date	2012-08-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218257-9
ISSN	1097-0215 ; 0020-7136
ISSN (online)	1097-0215
ISSN	0020-7136
DOI	10.1002/ijc.27731
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Zs.A 478: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Toxoplasma gondii modulates the host cell cycle, chromosome segregation, and cytokinesis irrespective of cell type or species origin.

Rojas-Baron, Lisbeth / Senk, Kira / Hermosilla, Carlos / Taubert, Anja / Velásquez, Zahady D

Parasites & vectors

2024 Volume 17, Issue 1, Page(s) 180

Abstract: Background: Toxoplasma gondii is an apicomplexan intracellular obligate parasite and the etiological agent of toxoplasmosis in humans, domestic animals and wildlife, causing miscarriages and negatively impacting offspring. During its intracellular ... ...

Abstract	Background: Toxoplasma gondii is an apicomplexan intracellular obligate parasite and the etiological agent of toxoplasmosis in humans, domestic animals and wildlife, causing miscarriages and negatively impacting offspring. During its intracellular development, it relies on nutrients from the host cell, controlling several pathways and the cytoskeleton. T. gondii has been proven to control the host cell cycle, mitosis and cytokinesis, depending on the time of infection and the origin of the host cell. However, no data from parallel infection studies have been collected. Given that T. gondii can infect virtually any nucleated cell, including those of humans and animals, understanding the mechanism by which it infects or develops inside the host cell is essential for disease prevention. Therefore, we aimed here to reveal whether this modulation is dependent on a specific cell type or host cell species. Methods: We used only primary cells from humans and bovines at a maximum of four passages to ensure that all cells were counted with appropriate cell cycle checkpoint control. The cell cycle progression was analysed using fluorescence-activated cell sorting (FACS)-based DNA quantification, and its regulation was followed by the quantification of cyclin B1 (mitosis checkpoint protein). The results demonstrated that all studied host cells except bovine colonic epithelial cells (BCEC) were arrested in the S-phase, and none of them were affected in cyclin B1 expression. Additionally, we used an immunofluorescence assay to track mitosis and cytokinesis in uninfected and T. gondii-infected cells. Results: The results demonstrated that all studied host cell except bovine colonic epithelial cells (BCEC) were arrested in the S-phase, and none of them were affected in cyclin B1 expression. Our findings showed that the analysed cells developed chromosome segregation problems and failed to complete cytokinesis. Also, the number of centrosomes per mitotic pole was increased after infection in all cell types. Therefore, our data suggest that T. gondii modulates the host cell cycle, chromosome segregation and cytokinesis during infection or development regardless of the host cell origin or type.
MeSH term(s)	Humans ; Animals ; Cattle ; Toxoplasma/physiology ; Cytokinesis ; Cyclin B1/genetics ; Cyclin B1/metabolism ; Chromosome Segregation ; Toxoplasmosis/parasitology
Chemical Substances	Cyclin B1
Language	English
Publishing date	2024-04-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2409480-8
ISSN	1756-3305 ; 1756-3305
ISSN (online)	1756-3305
ISSN	1756-3305
DOI	10.1186/s13071-024-06244-2
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Article: New Insights on the Neglected Avian Nematode

Fischer, Ella F / Schlohsarczyk, Elfi K / Gröf, Manuela / Gärtner, Ulrich / Taubert, Anja / Hermosilla, Carlos

Pathogens (Basel, Switzerland)

2023 Volume 12, Issue 5

Abstract: Hystrichis ... ...

Abstract	Hystrichis tricolor
Language	English
Publishing date	2023-04-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens12050663
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Book: Wann sollte im ärztlichen Alltag an einen HIV-Test gedacht werden?

Esser, Stefan / Schüttfort, Gundolf / Taubert, Steffen / Lemmen, Karl / Rockstroh, Jürgen

ein Handlungsleitfaden

(Praxis Report ; 11. Jahrgang, Heft 2 (März 2019))

2019

Author's details	Herausgeber Prof. Dr. Jürgen K. Rockstroh ; Autoren PD Dr. med. Stefan Esser, Dr. Dr. Gundolf Schüttfort, Dipl.-Psych. Steffen Taubert, Dipl.-Psych. Karl Lemmen
Series title	Praxis Report ; 11. Jahrgang, Heft 2 (März 2019)
Collection
Language	German
Size	19 Seiten, Illustrationen
Publisher	Thieme
Publishing place	Stuttgart
Publishing country	Germany
Document type	Book
HBZ-ID	HT020105267
Database	Catalogue ZB MED Medicine, Health

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Zs A 6809 -11,2-	verfügbar in Königswinter	Königswinter

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Article ; Online: Iron coatings on carbonate rocks shape the attached bacterial aquifer community.

Sharma, Alisha / Taubert, Martin / Pérez-Carrascal, Olga M / Lehmann, Robert / Ritschel, Thomas / Totsche, Kai U / Lazar, Cassandre S / Küsel, Kirsten

The Science of the total environment

2024 Volume 917, Page(s) 170384

Abstract: Most studies of groundwater ecosystems target planktonic microbes, which are easily obtained via water samples. In contrast, little is known about the diversity and function of microbes adhering to rock surfaces, particularly to consolidated rocks. To ... ...

Abstract	Most studies of groundwater ecosystems target planktonic microbes, which are easily obtained via water samples. In contrast, little is known about the diversity and function of microbes adhering to rock surfaces, particularly to consolidated rocks. To investigate microbial attachment to rock surfaces, we incubated rock chips from fractured aquifers in limestone-mudstone alternations in bioreactors fed with groundwater from two wells representing oxic and anoxic conditions. Half of the chips were coated with iron oxides, representing common secondary mineralization in fractured rock. Our time-series analysis showed bacteria colonizing the chips within two days, reaching cell numbers up to 4.16 × 10
MeSH term(s)	Iron ; Ecosystem ; Bacteria ; Carbonates ; Groundwater/microbiology
Chemical Substances	Iron (E1UOL152H7) ; Carbonates
Language	English
Publishing date	2024-01-26
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.170384
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Article ; Conference proceedings: Ist Sarkopenie ein nützlicher Bildgebungs-Biomarker bei Patienten mit primär sklerosierender Cholangitis (PSC)?

Ringe, K / Levers, A / Pantke, J / Düx, D / Klimes, F / Taubert, R / Wedemeyer, H H / Lenzen, H / Wacker, F

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren

2024 Volume 196, Issue S 01

Event/congress	RÖKO 2024, RheinMain CongressCenter (RMCC) in Wiesbaden, 2024-05-08
Language	German
Publishing date	2024-04-01
Publisher	Georg Thieme Verlag
Publishing place	Stuttgart ; New York
Document type	Article ; Conference proceedings
ZDB-ID	554830-5
ISSN	1438-9010 ; 1438-9029 ; 0340-1618 ; 0936-6652 ; 1433-5972
ISSN (online)	1438-9010
ISSN	1438-9029 ; 0340-1618 ; 0936-6652 ; 1433-5972
DOI	10.1055/s-0044-1781490
Database	Thieme publisher's database

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Article ; Online: Understanding adefovir pharmacokinetics as a component of a transporter phenotyping cocktail.

Dong, Qian / Chen, Chunli / Taubert, Max / Bilal, Muhammad / Kinzig, Martina / Sörgel, Fritz / Scherf-Clavel, Oliver / Fuhr, Uwe / Dokos, Charalambos

European journal of clinical pharmacology

2024

Abstract: ... observed for adefovir as a victim. However, renal elimination remained unaffected. The high K ...

Abstract	Purpose: Adefovir (as dipivoxil) was selected as a probe drug in a previous transporter cocktail phenotyping study to assess renal organic anion transporter 1 (OAT1), with renal clearance (CL Methods: Data from 24 healthy subjects were reanalyzed. After establishing a base model, covariate effects, including the impact of co-administered drugs, were assessed using forward inclusion then backward elimination. Results: A one-compartment model with first-order absorption (including lag time) and a combination of nonlinear renal and linear nonrenal elimination best described the data. A significantly higher apparent bioavailability (73.6% vs. 59.0%) and a lower apparent absorption rate constant (2.29 h Conclusion: The popPK modeling approach indicated that the co-administration primarily affected the apparent absorption and/or prodrug conversion of adefovir dipivoxil, resulting in the minor drug-drug interaction observed for adefovir as a victim. However, renal elimination remained unaffected. The high K
Language	English
Publishing date	2024-03-28
Publishing country	Germany
Document type	Journal Article
ZDB-ID	121960-1
ISSN	1432-1041 ; 0031-6970
ISSN (online)	1432-1041
ISSN	0031-6970
DOI	10.1007/s00228-024-03673-x
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