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  1. Article: The role of interleukin 6 in the pathogenesis and therapy of Castleman disease - an immunologist's perspective.

    Milota, Tomáš / Střížová, Zuzana / Sobotková, Marta / Bartůňková, Jiřina

    Casopis lekaru ceskych

    2023  Volume 162, Issue 2-3, Page(s) 106–111

    Abstract: Castleman disease (CD) is a heterogeneous group of diseases characterized by lymphadenopathy and systemic inflammatory manifestations. CD can be divided into uni- (UCD) and multicentric form (MCD) according to the disease extent. MCD is usually ... ...

    Title translation Úloha interleukinu 6 v patogenezi a léčbě Castlemanovy choroby – pohled imunologa.
    Abstract Castleman disease (CD) is a heterogeneous group of diseases characterized by lymphadenopathy and systemic inflammatory manifestations. CD can be divided into uni- (UCD) and multicentric form (MCD) according to the disease extent. MCD is usually accompanied by the features of a systemic inflammatory response including fever, weight loss, hepatosplenomegaly, ascites, and edema. In these patients, we can also observe elevation of inflammatory parameters and anemia within the laboratory assessment. Based on etiological nature, the CD can be further divided into human herpesvirus-8-associated (HHV8-associated) and idiopathic form. Interleukin 6 (IL-6) plays a central role in the disease pathogenesis. Inhibition of IL-6 has been shown to be an effective treatment modality. Currently, siltuximab, a chimeric monoclonal antibody targeting IL-6, is the only approved treatment for MCD. Its short-term and long-term efficacy and safety have been demonstrated in a few clinical studies.
    MeSH term(s) Humans ; Castleman Disease/drug therapy ; Castleman Disease/pathology ; Interleukin-6/therapeutic use ; Treatment Outcome
    Chemical Substances Interleukin-6 ; IL6 protein, human
    Language English
    Publishing date 2023-07-18
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 413441-2
    ISSN 1805-4420 ; 0008-7335
    ISSN (online) 1805-4420
    ISSN 0008-7335
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  2. Article: Current treatment options for monogenic periodic fever syndromes - the role of interleukin 1 inhibitors.

    Milota, Tomáš / Malcová, Hana / Střížová, Zuzana / Cebecauerová, Dita / Horváth, Rudolf

    Casopis lekaru ceskych

    2022  Volume 161, Issue 1, Page(s) 3–10

    Abstract: Monogenic periodic fever syndromes are heterogeneous group of autoinflammatory diseases including distinct syndromes, such as cryopyrin-associated periodic syndrome (CAPS), tumor necrosis factor alpha receptor-associated periodic syndrome (TRAPS), ... ...

    Title translation Současné možnosti léčby monogenních syndromů periodických horeček - úloha inhibitorů interleukinu 1.
    Abstract Monogenic periodic fever syndromes are heterogeneous group of autoinflammatory diseases including distinct syndromes, such as cryopyrin-associated periodic syndrome (CAPS), tumor necrosis factor alpha receptor-associated periodic syndrome (TRAPS), mevalonate kinase deficiency/hyper IgD syndrome (MKD/HIDS), and familial Mediterranean fever (FMF). Individual diseases differ in pathogenesis, clinical manifestations, and severity. However, cytokines from the interleukin 1 (IL-1) family play a key role in all of them. Inhibition of these cytokines, especially IL-1, thus plays a crucial role in their treatment. At present, we have a wide range of drugs that differ in structure, mechanism of action, efficacy, and spectrum of side effects. The most available are anakinra, canakinumab and rilonacept. Moreover, several clinical trials are currently underway with other very promising drugs, such as gevokizumab, tadekinig alfa or tranilast. In the following review, we provide a new perspective on the efficacy and safety of IL-1 inhibitors that have provided the novel results coming from recently published clinical trials.
    MeSH term(s) Cryopyrin-Associated Periodic Syndromes/drug therapy ; Hereditary Autoinflammatory Diseases/drug therapy ; Humans ; Interleukin Inhibitors ; Interleukin-1/therapeutic use ; Mevalonate Kinase Deficiency/drug therapy
    Chemical Substances Interleukin Inhibitors ; Interleukin-1
    Language English
    Publishing date 2022-03-30
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 413441-2
    ISSN 1805-4420 ; 0008-7335
    ISSN (online) 1805-4420
    ISSN 0008-7335
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  3. Article: Principles and new perspectives in the vaccination against SARS-CoV-2 virus.

    Smetanová, Jitka / Střížová, Zuzana / Bartůňková, Jiřina / Milota, Tomáš

    Casopis lekaru ceskych

    2021  Volume 159, Issue 7-8, Page(s) 298–302

    Abstract: It has been a year since the first person on Earth became infected with a new type of coronavirus SARS-CoV-2, causing infectious acute respiratory disease COVID-19 with relatively high morbidity and mortality. The most endangered population by ... ...

    Title translation Principy a výhledy vakcinace proti viru SARS-CoV-2.
    Abstract It has been a year since the first person on Earth became infected with a new type of coronavirus SARS-CoV-2, causing infectious acute respiratory disease COVID-19 with relatively high morbidity and mortality. The most endangered population by coronavirus SARS-CoV-2 are healthcare professionals, the elderly and people with associated comorbidities. Due to the fast community spread, governments of different European countries introduced precaution measures including limited socializing of people, closing of most public services and introducing mandatory facial protection. The hope for a return to the life before the pandemic is the development of an effective and safe vaccine against SARS-CoV-2 which would presumably reduce the incidence of severe forms of COVID-19 and prevent the massive spread of the disease. At the end of November, we have 13 clinical trials in phase III involving SARS-CoV-2 vaccines based on inactivated viruses, recombinant non-pathogenic viral vectors and proteins. The first mRNA-based vaccine is currently being evaluated in phase II/III clinical trial and is already being distributed and applied to high-risk population in the United Kingdom, the United States, and Israel, followed by the countries of the European Union, including the Czech Republic. In the review article we present currently ongoing clinical studies with a special focus on the phase III clinical trials and discuss the mechanisms of action of each type of vaccine.
    MeSH term(s) Aged ; COVID-19 ; COVID-19 Vaccines ; Czech Republic ; Europe ; Humans ; SARS-CoV-2 ; United Kingdom ; Vaccination
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2021-01-12
    Publishing country Czech Republic
    Document type Journal Article ; Review
    ZDB-ID 413441-2
    ISSN 1805-4420 ; 0008-7335
    ISSN (online) 1805-4420
    ISSN 0008-7335
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  4. Article ; Online: Immunophenotyping of peripheral blood in NSCLC patients discriminates responders to immune checkpoint inhibitors.

    Krizova, Ludmila / Benesova, Iva / Zemanova, Petra / Spacek, Jan / Strizova, Zuzana / Humlova, Zuzana / Mikulova, Veronika / Petruzelka, Lubos / Vocka, Michal

    Journal of cancer research and clinical oncology

    2024  Volume 150, Issue 2, Page(s) 99

    Abstract: Purpose: Immune checkpoint inhibitors (ICIs) dramatically changed the prognosis of patients with NSCLC. Unfortunately, a reliable predictive biomarker is still missing. Commonly used biomarkers, such as PD-L1, MSI, or TMB, are not quite accurate in ... ...

    Abstract Purpose: Immune checkpoint inhibitors (ICIs) dramatically changed the prognosis of patients with NSCLC. Unfortunately, a reliable predictive biomarker is still missing. Commonly used biomarkers, such as PD-L1, MSI, or TMB, are not quite accurate in predicting ICI efficacy.
    Methods: In this prospective observational cohort study, we investigated the predictive role of erythrocytes, thrombocytes, innate and adaptive immune cells, complement proteins (C3, C4), and cytokines from peripheral blood of 224 patients with stage III/IV NSCLC treated with ICI alone (pembrolizumab, nivolumab, and atezolizumab) or in combination (nivolumab + ipilimumab) with chemotherapy. These values were analyzed for associations with the response to the treatment and survival endpoints.
    Results: Higher baseline Tregs, MPV, hemoglobin, and lower monocyte levels were associated with favorable PFS and OS. Moreover, increased baseline basophils and lower levels of C3 predicted significantly improved PFS. The levels of the baseline immature granulocytes, C3, and monocytes were significantly associated with the occurrence of partial regression at the first restaging. Multiple studied parameters (n = 9) were related to PFS benefit at the time of first restaging as compared to baseline values. In addition, PFS nonbenefit group showed a decrease in lymphocyte count after three months of therapy. The OS benefit was associated with higher levels of lymphocytes, erythrocytes, hemoglobin, MCV, and MPV, and a lower value of NLR after three months of treatment.
    Conclusion: Our work suggests that parameters from peripheral venous blood may be potential biomarkers in NSCLC patients on ICI. The baseline values of Tregs, C3, monocytes, and MPV are especially recommended for further investigation.
    MeSH term(s) Humans ; Lung Neoplasms ; Nivolumab/therapeutic use ; Immune Checkpoint Inhibitors/therapeutic use ; Immunophenotyping ; Prospective Studies ; Antineoplastic Agents, Immunological/therapeutic use ; Carcinoma, Non-Small-Cell Lung ; Biomarkers ; Hemoglobins/therapeutic use ; B7-H1 Antigen
    Chemical Substances Nivolumab (31YO63LBSN) ; Immune Checkpoint Inhibitors ; Antineoplastic Agents, Immunological ; Biomarkers ; Hemoglobins ; B7-H1 Antigen
    Language English
    Publishing date 2024-02-21
    Publishing country Germany
    Document type Observational Study ; Journal Article
    ZDB-ID 134792-5
    ISSN 1432-1335 ; 0171-5216 ; 0084-5353 ; 0943-9382
    ISSN (online) 1432-1335
    ISSN 0171-5216 ; 0084-5353 ; 0943-9382
    DOI 10.1007/s00432-024-05628-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Diagnostic challenges and treatment options in patients with solitary fibrous tumor: A single-center observational study.

    Ozaniak, Andrej / Hladik, Pavel / Lischke, Robert / Strizova, Zuzana

    Frontiers in surgery

    2022  Volume 9, Page(s) 952463

    Abstract: Introduction: Solitary fibrous tumor (SFT) is an extremely rare disease with a high misdiagnosis rate and a potentially malignant biologic nature. We have collected and analyzed data from 18 SFT patients to provide a deeper insight into this uncommon ... ...

    Abstract Introduction: Solitary fibrous tumor (SFT) is an extremely rare disease with a high misdiagnosis rate and a potentially malignant biologic nature. We have collected and analyzed data from 18 SFT patients to provide a deeper insight into this uncommon disease entity.
    Methods: In our study, 18 patients who had undergone surgery between April 2014 and December 2021 for the diagnosis of SFT were evaluated. The collected data for each patient included the location of the SFT, the preoperative diagnosis, the definitive histological diagnosis, the presence of postoperative complications, the time of recurrence, the time of systemic progression, the type of treatment, and the survival rate. The median follow-up was 36 months.
    Results: In three patients, the preoperative diagnosis did not correlate with the definitive histology of SFT. In patients with the limb location of SFT, no signs of recurrence nor distant metastases were seen within the study period. In total, 50% of the postsurgical complications were associated with the abdominal location of the SFT. In newly diagnosed SFT patients, two patients (20%) developed local recurrence, and the median time until recurrence was 22.5 months. Out of patients that were admitted and operated on for recurrent SFT, 67% relapsed, and the median time to relapse was 9.5 months. The systemic progression of the disease was observed in 33% of patients treated for recurrent SFT.
    Conclusion: In our study, the misdiagnosis rate was high and correlated with previously published studies. Postsurgical complications were associated with the extrathoracic location of SFT. The mainstay of SFT treatment remains radical surgery, although radiotherapy alone can significantly improve overall survival. Clinical trials are urgently needed to evaluate the potential effect of other treatment modalities, such as immunotherapy and targeted therapy, in SFT patients.
    Language English
    Publishing date 2022-08-31
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2773823-1
    ISSN 2296-875X
    ISSN 2296-875X
    DOI 10.3389/fsurg.2022.952463
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  6. Article: Can wearing face masks in public affect transmission route and viral load in COVID-19?

    Střížová, Zuzana / Bartůňková, Jiřina / Smrž, Daniel

    Central European journal of public health

    2020  Volume 28, Issue 2, Page(s) 161–162

    Abstract: The mandatory face mask wearing was implemented in the Czech Republic and Slovakia shortly after the COVID-19 outbreak in Central Europe. So far, the number of COVID-19-associated deaths per 100,000 individuals is far lower in these countries as compared ...

    Abstract The mandatory face mask wearing was implemented in the Czech Republic and Slovakia shortly after the COVID-19 outbreak in Central Europe. So far, the number of COVID-19-associated deaths per 100,000 individuals is far lower in these countries as compared with other neighbouring or close countries. The use of face masks in public may not protect the general public from contracting the virus, however, presumptively decreases the viral load and contributes to a favourable clinical outcome in COVID-19 disease. A certain time is required for antigen-specific T cells and B cells to fully develop. Obligatory face mask wearing in public favours the virus transmission through oral mucosa and/or conjunctival epithelium, which enables the adaptive immune responses to evolve. In the case of inhalation of high loads of SARS-CoV-2, the time for the development of fully protective adaptive immune responses seems to be insufficient. Then, a less specific and more damaging innate immune response prevails.
    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections/epidemiology ; Coronavirus Infections/prevention & control ; Coronavirus Infections/transmission ; Czech Republic/epidemiology ; Humans ; Masks ; Pandemics/prevention & control ; Personal Protective Equipment ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/prevention & control ; Pneumonia, Viral/transmission ; Protective Clothing ; SARS-CoV-2 ; Slovakia/epidemiology ; Viral Load
    Keywords covid19
    Language English
    Publishing date 2020-06-27
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 1176053-9
    ISSN 1803-1048 ; 1210-7778 ; 0022-1732
    ISSN (online) 1803-1048
    ISSN 1210-7778 ; 0022-1732
    DOI 10.21101/cejph.a6290
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  7. Article: COVID-19 from the perspective of an immunologist.

    Střížová, Zuzana / Milota, Tomáš / Bartůňková, Jiřina

    Casopis lekaru ceskych

    2020  Volume 159, Issue 2, Page(s) 67–71

    Abstract: COVID-19 is an infectious disease caused by a coronavirus SARS-CoV-2. COVID-19 has a number of similarities to SARS and MERS diseases. Its highly contagious nature is particularly due to the rapid spread of the disease through asymptomatic individuals; ... ...

    Title translation Aktuální problematika COVID-19 z pohledu imunologa.
    Abstract COVID-19 is an infectious disease caused by a coronavirus SARS-CoV-2. COVID-19 has a number of similarities to SARS and MERS diseases. Its highly contagious nature is particularly due to the rapid spread of the disease through asymptomatic individuals; however, the worlds most contagious infectious disease is still considered measles. Scientific data have revealed the interactions between COVID-19 and the immune system. These findings may contribute to the development of novel preventive and therapeutic approaches. Just as coronavirus itself the reports about the disease have massively spread through media and public contributing to overall public fear and stress. This promotion of non-scientific evidence and misinformation through social media might have also a devastating impact on the individuals immune system. Data regarding the mortality rates of COVID-19 have achieved unprecedented media and public engagements, however, the true facts about the disease prevention, immunomodulation and novel treatments are often left unsaid. We present the most recent facts about COVID-19 disease and its interactions with the immune system.
    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections/immunology ; Humans ; Immune System ; Pandemics ; Pneumonia, Viral/immunology ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-06-03
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 413441-2
    ISSN 1805-4420 ; 0008-7335
    ISSN (online) 1805-4420
    ISSN 0008-7335
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  8. Article: News in immunology.

    Bartůňková, Jiřina / Bloomfield, Markéta / Havlišová, Magdalena / Klocperk, Adam / Kubešová, Helena / Podrazil, Michal / Střížová, Zuzana / Šedivá, Anna

    Vnitrni lekarstvi

    2023  Volume 69, Issue 2, Page(s) 133–137

    Abstract: The field of immunology has undergone a very significant development in recent decades, which has been reflected especially in the beginning of this millennium in significant advances in the understanding of the immune system and in the application of ... ...

    Title translation Novinky v imunologii.
    Abstract The field of immunology has undergone a very significant development in recent decades, which has been reflected especially in the beginning of this millennium in significant advances in the understanding of the immune system and in the application of this knowledge in practice. The progress and acceleration of research and advances in the field of immunology was further prompt by the unexpected onset of the COVID-19 pandemic in 2020. The intense scientific work has not only led to the development of our understanding of the immune response to viruses, but also to the rapid conversion of this knowledge into practical pandemic management on a global scale, as exemplified by the development of vaccines against SARS-Cov-2 virus. The pandemic era has further contributed to the acceleration of the application of not only biological discoveries but also technological approaches into practical applications, such as use of advanced mathematics, computer science and, more recently, artificial intelligence which are all are adding to the advances that are significantly moving the field of immunology forward. In this communication, we present specific advances in particular areas of immunopathology, which are mainly allergy, immunodeficiency, immunity and infection, vaccination, autoimmune diseases and cancer immunology.
    MeSH term(s) Humans ; COVID-19 ; SARS-CoV-2 ; COVID-19 Vaccines ; Pandemics ; Artificial Intelligence
    Chemical Substances COVID-19 Vaccines
    Language English
    Publishing date 2023-04-18
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 138213-5
    ISSN 1801-7592 ; 0042-773X
    ISSN (online) 1801-7592
    ISSN 0042-773X
    DOI 10.36290/vnl.2023.021
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  9. Article: Rare Pseudosarcomatous Lesions Posing Diagnostic Challenges: Histopathologic Examination as a Dominant Tool Preventing Misdiagnosis of Proliferative Fasciitis.

    Ozaniak, Andrej / Vachtenheim, Jiri / Chmelova, Renata / Lischke, Robert / Strizova, Zuzana

    Cureus

    2022  Volume 14, Issue 6, Page(s) e25770

    Abstract: Proliferative fasciitis is an extremely rare benign myofibroblastic proliferation that typically presents as a rapidly growing subcutaneous mass. Precise histopathological interpretation is required to obtain a proper diagnosis. Due to a symptomatology ... ...

    Abstract Proliferative fasciitis is an extremely rare benign myofibroblastic proliferation that typically presents as a rapidly growing subcutaneous mass. Precise histopathological interpretation is required to obtain a proper diagnosis. Due to a symptomatology overlap, discrimination from soft tissue sarcomas is crucial in the prevention of unnecessary excessive treatment that could be potentially harmful to the patients. Here, we present a rare case of atypical localization of proliferative fasciitis. The lesion was predominantly localized in the groin with the invasion of the scrotum and clinically mimicked soft tissue sarcoma. However, according to a proper histopathologic analysis, the diagnosis of proliferative fasciitis was concluded. With a large number of pseudosarcomatous lesions, there is a rising urge to introduce these rare but benign processes to physicians in order to prevent misdiagnosing patients.
    Language English
    Publishing date 2022-06-08
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2747273-5
    ISSN 2168-8184
    ISSN 2168-8184
    DOI 10.7759/cureus.25770
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  10. Article: How COVID-19 Affects Lung Transplantation: A Comprehensive Review.

    Vachtenheim, Jiri / Novysedlak, Rene / Svorcova, Monika / Lischke, Robert / Strizova, Zuzana

    Journal of clinical medicine

    2022  Volume 11, Issue 12

    Abstract: Lung transplant (LuTx) recipients are at a higher risk of developing serious illnesses from COVID-19, and thus, we have closely reviewed the consequences of the COVID-19 pandemic on lung transplantation. In most transplant centers, the overall LuTx ... ...

    Abstract Lung transplant (LuTx) recipients are at a higher risk of developing serious illnesses from COVID-19, and thus, we have closely reviewed the consequences of the COVID-19 pandemic on lung transplantation. In most transplant centers, the overall LuTx activity significantly declined and led to a specific period of restricting lung transplantation to urgent cases. Moreover, several transplant centers reported difficulties due to the shortage of ICU capacities. The fear of donor-derived transmission generated extensive screening programs. Nevertheless, reasonable concerns about the unnecessary losses of viable organs were also raised. The overall donor shortage resulted in increased waiting-list mortality, and COVID-19-associated ARDS became an indication of lung transplantation. The impact of specific immunosuppressive agents on the severity of COVID-19 varied. Corticosteroid discontinuation was not found to be beneficial for LuTx patients. Tacrolimus concentrations were reported to increase during the SARS-CoV-2 infection, and in combination with remdesivir, tacrolimus may clinically impact renal functions. Monoclonal antibodies were shown to reduce the risk of hospitalization in SOT recipients. However, understanding the pharmacological interactions between the anti-COVID-19 drugs and the immunosuppressive drugs requires further research.
    Language English
    Publishing date 2022-06-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm11123513
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