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Article ; Online: Building a framework for embryonic microenvironments and cancer stem cells.

Ruiz-Vela, Antonio / Aguilar-Gallardo, Cristóbal / Simón, Carlos

2010 Volume 5, Issue 4, Page(s) 319–327

Abstract: The putative existence of a cancer stem cell niche consisting of bi-directional stromal and stem cell secreting factors that trigger cancer stem cell growth and proliferation has been hypothesized in the nervous and hematopoietic systems. In light of ... ...

Abstract	The putative existence of a cancer stem cell niche consisting of bi-directional stromal and stem cell secreting factors that trigger cancer stem cell growth and proliferation has been hypothesized in the nervous and hematopoietic systems. In light of this theory, it has been proposed that embryonic stem cell microenvironments, upon interactions with cancer stem cells, may reprogram cancer cells resulting in a substantial inhibition of tumor cell properties. Here, we discuss emerging data that support this novel concept of cancer inhibitory factors produced in the context of embryonic microenvironments as well as by embryonic stem cells (ESCs).
MeSH term(s)	Animals ; Biomarkers ; Cell Differentiation ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Humans ; Neoplasms/therapy ; Neoplastic Stem Cells/cytology ; Neoplastic Stem Cells/metabolism
Chemical Substances	Biomarkers
Language	English
Publishing date	2010-01-08
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2495577-2
ISSN	2629-3277 ; 1558-6804 ; 1550-8943
ISSN (online)	2629-3277 ; 1558-6804
ISSN	1550-8943
DOI	10.1007/s12015-009-9096-7
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Article: Sleep quality and sleep deprivation: relationship with academic performance in university students during examination period.

Suardiaz-Muro, Maria / Ortega-Moreno, Manuel / Morante-Ruiz, Miguel / Monroy, Manuel / Ruiz, Miguel A / Martín-Plasencia, Pilar / Vela-Bueno, Antonio

Sleep and biological rhythms

2023 Volume 21, Issue 3, Page(s) 377–383

Abstract: The beginning of the university brings together maturational, psychosocial and academic changes that make university students more prone to suffer from insufficient or poor quality sleep, which can negatively influence their academic performance. The ... ...

Abstract	The beginning of the university brings together maturational, psychosocial and academic changes that make university students more prone to suffer from insufficient or poor quality sleep, which can negatively influence their academic performance. The period of taking exams is a key part of the academic year. However, there are few studies that analyze sleep during this period of time. Our aim is to study the association of sleep quality and sleep deprivation with academic performance during the examination period. A descriptive, cross-sectional and correlational study was carried out with the participation of 640 subjects in the first three years of five faculties belonging to the Universidad Autónoma de Madrid. The instrument used consisted of a questionnaire that included sociodemographic and academic data, Pittsburgh Sleep Quality Index, Epworth Sleepiness Scale and information about the academic performance. During the examination period, a positive association was found between sleep quality and academic performance. University students slept less than desired, both on weekdays and weekends, and the sleep debt during the week was associated with a worse students' perception of their academic performance. In total, 61.3% of the students believed that their performance would improve by getting more sleep. In addition, low drowsiness and napping were also found. In conclusion, during periods of greater academic demand, an insufficient sleep and poor quality is commonly observed, affecting negatively to their academic performance. Actually, about 2/3 of our subjects believed that their performance would improve by getting more sleep.
Language	English
Publishing date	2023-04-10
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2185804-4
ISSN	1479-8425 ; 1446-9235
ISSN (online)	1479-8425
ISSN	1446-9235
DOI	10.1007/s41105-023-00457-1
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Article: Proapoptotic histone H1.2 induces CASP-3 and -7 activation by forming a protein complex with CYT c, APAF-1 and CASP-9.

Ruiz-Vela, Antonio / Korsmeyer, Stanley J

FEBS letters

2007 Volume 581, Issue 18, Page(s) 3422–3428

Abstract: Cytochrome c (CYT c) is a protein that employs the caspase recruitment domain (CARD)-containing proteins APAF-1 and CASP-9 to activate effectors CASP-3 and -7. By using affinity labeling techniques and mass spectrometry analysis, we show that histone H1 ... ...

Abstract	Cytochrome c (CYT c) is a protein that employs the caspase recruitment domain (CARD)-containing proteins APAF-1 and CASP-9 to activate effectors CASP-3 and -7. By using affinity labeling techniques and mass spectrometry analysis, we show that histone H1.2 is a regulator of caspases upon UV irradiation. We demonstrated that histone H1.2 forms a protein complex with APAF-1, CASP-9 and CYT c upon UV irradiation. In cell-free systems, we show that histone H1.2 triggers activation of CASP-3 and -7 via APAF-1 and CASP-9. We therefore conclude that upon DNA damage histone H1.2 acts as a positive regulator of apoptosome formation.
MeSH term(s)	Animals ; Apoptosis ; Apoptotic Protease-Activating Factor 1/deficiency ; Apoptotic Protease-Activating Factor 1/genetics ; Apoptotic Protease-Activating Factor 1/metabolism ; Caspase 3/metabolism ; Caspase 7/metabolism ; Caspase 9/deficiency ; Caspase 9/genetics ; Caspase 9/metabolism ; Cells, Cultured ; Cytochromes c/metabolism ; Enzyme Activation ; Histones/metabolism ; Mice ; Mice, Knockout ; Protein Binding
Chemical Substances	Apoptotic Protease-Activating Factor 1 ; Histones ; Cytochromes c (9007-43-6) ; Caspase 3 (EC 3.4.22.-) ; Caspase 7 (EC 3.4.22.-) ; Caspase 9 (EC 3.4.22.-)
Language	English
Publishing date	2007-07-24
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1016/j.febslet.2007.06.049
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Article ; Online: Endothelial progenitor cells as biomarkers of diabetes-related cardiovascular complications.

Benítez-Camacho, Josefa / Ballesteros, Antonio / Beltrán-Camacho, Lucía / Rojas-Torres, Marta / Rosal-Vela, Antonio / Jimenez-Palomares, Margarita / Sanchez-Gomar, Ismael / Durán-Ruiz, Mª Carmen

Stem cell research & therapy

2023 Volume 14, Issue 1, Page(s) 324

Abstract: Diabetes mellitus (DM) constitutes a chronic metabolic disease characterized by elevated levels of blood glucose which can also lead to the so-called diabetic vascular complications (DVCs), responsible for most of the morbidity, hospitalizations and ... ...

Abstract	Diabetes mellitus (DM) constitutes a chronic metabolic disease characterized by elevated levels of blood glucose which can also lead to the so-called diabetic vascular complications (DVCs), responsible for most of the morbidity, hospitalizations and death registered in these patients. Currently, different approaches to prevent or reduce DM and its DVCs have focused on reducing blood sugar levels, cholesterol management or even changes in lifestyle habits. However, even the strictest glycaemic control strategies are not always sufficient to prevent the development of DVCs, which reflects the need to identify reliable biomarkers capable of predicting further vascular complications in diabetic patients. Endothelial progenitor cells (EPCs), widely known for their potential applications in cell therapy due to their regenerative properties, may be used as differential markers in DVCs, considering that the number and functionality of these cells are affected under the pathological environments related to DM. Besides, drugs commonly used with DM patients may influence the level or behaviour of EPCs as a pleiotropic effect that could finally be decisive in the prognosis of the disease. In the current review, we have analysed the relationship between diabetes and DVCs, focusing on the potential use of EPCs as biomarkers of diabetes progression towards the development of major vascular complications. Moreover, the effects of different drugs on the number and function of EPCs have been also addressed.
MeSH term(s)	Humans ; Endothelial Progenitor Cells/metabolism ; Diabetes Mellitus/metabolism ; Diabetic Angiopathies/metabolism ; Blood Glucose/metabolism ; Biomarkers/metabolism
Chemical Substances	Blood Glucose ; Biomarkers
Language	English
Publishing date	2023-11-10
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-023-03537-8
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Article ; Online: Modulating Elements of Nurse Resilience in Population Care during the COVID-19 Pandemic.

Sierra-García, Ester / Sosa-Palanca, Eva María / Saus-Ortega, Carlos / Ruiz-Hontangas, Antonio / Juárez-Vela, Raúl / Gea-Caballero, Vicente

International journal of environmental research and public health

2022 Volume 19, Issue 8

Abstract: COVID-19 has significantly affected the work environment of nurses. In the face of the challenges posed by stressors in clinical practice, some nurses adapt and prove to be resilient. In the face of the COVID-19 pandemic, the nature of care itself and ... ...

Abstract	COVID-19 has significantly affected the work environment of nurses. In the face of the challenges posed by stressors in clinical practice, some nurses adapt and prove to be resilient. In the face of the COVID-19 pandemic, the nature of care itself and the new ways of working are potentially very stressful. We aim to analyze the resilience of care nurses to the psychological impact of the COVID-19 pandemic. This study is a systematic review of nurse caregiver resilience to the COVID-19 pandemic in 2021. Our search was conducted in the WOS, Medline/PubMed, Cochrane, BVS/LILACS, and Cuiden databases. The inclusion criteria were: studies published in Spanish or English; carried out from March 2020 to May 2021 on nurses caring for patients with COVID-19; and investigating the factors influencing the psychological impact, resilience, strategies to develop it, and interventions to promote it during this pandemic and others, such as SARS, MERS, or ebola. The quality of the studies and the risk of bias were evaluated following ICROMS, STROBE and AMSTAR-2 criteria. Twenty-two studies were selected. Most of the studies highlighted the presence of stressors in nurses, emphasizing those of the environment, which converged in dysfunctional responses that hurt their resilience. The most persuasive factors were social and organizational support. Coping strategies developed by nurses and especially interventions by organizations were detected as instruments to foster resilience, but have not been well researched. Resilience has a key moderating role in mitigating the psychological impact of nurses in the face of the COVID-19 pandemic.
MeSH term(s)	Adaptation, Psychological ; COVID-19/epidemiology ; Humans ; Pandemics ; Resilience, Psychological ; SARS-CoV-2
Language	English
Publishing date	2022-04-07
Publishing country	Switzerland
Document type	Journal Article ; Review ; Systematic Review
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph19084452
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Article ; Online: Profile and characteristics of the adequacy of blood transfusions in Trauma Intensive Care. A cross sectional multicenter study.

Juárez-Vela, Raúl / Quintana-Diaz, Manuel / Rodríguez-Calvo, Antonio / Santos-Sánchez, José Ángel / Gero-Escapa, María / Gallego-Curto, Elena / Satústegui-Dordá, Pedro José / Sánchez-González, Juan Luis / Jericó, Carlos / Ruiz de Viñaspre-Hernández, Regina / Gil-Fernández, Guadalupe / García-Erce, José Antonio

Frontiers in public health

2023 Volume 11, Page(s) 1133191

Abstract: Introduction: Major trauma is one of the major health care problems facing modern society, trauma systems require careful planning to achieve an ideal level of coverage for the population. The Patient Blood Management Program is an integrated and global ...

Abstract	Introduction: Major trauma is one of the major health care problems facing modern society, trauma systems require careful planning to achieve an ideal level of coverage for the population. The Patient Blood Management Program is an integrated and global strategy to provide patient care that aims to assess and address, when possible, the etiology of blood abnormalities rather than transfuse without treating the underlying cause. We aimed to describe the factors that are associated with the clinical decision to transfuse polytraumatized patients admitted to the Intensive Care Unit (ICU). Method: We performed a cross sectional multicenter study of patients admitted to ICUs for trauma in 14 Spanish hospitals from September 2020 to December 2021. Results: A total of 69 patients were treated in the emergency room due to polytrauma, 46% of them were considered serious in the initial triage. Thirty were caused by a fall from considerable height (43.47%), followed by 39 patients admitted due to trac accidents (56.52%). The location of the trauma was mainly cranioencephalic, followed by thoracic trauma. Of the 69 patients, 25 received a blood transfusion during their ICU stay (36.23%). Discussion: No significant differences were observed between transfused and non-transfused patients, except for the severity scales, where transfused patients have a higher score on all the scales assessed in the ICU except for the Revised Trauma Score. As we can see, the incidence of kidney failure was also different between the groups analyzed, reaching 44.00% in transfused patients and 13.64% in the group of patients without blood transfusion,
MeSH term(s)	Humans ; Cross-Sectional Studies ; Blood Transfusion ; Critical Care ; Intensive Care Units ; Hospitalization
Language	English
Publishing date	2023-03-20
Publishing country	Switzerland
Document type	Multicenter Study ; Journal Article
ZDB-ID	2711781-9
ISSN	2296-2565 ; 2296-2565
ISSN (online)	2296-2565
ISSN	2296-2565
DOI	10.3389/fpubh.2023.1133191
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Article ; Online: Endothelial progenitor cells as biomarkers of diabetes-related cardiovascular complications

Josefa Benítez-Camacho / Antonio Ballesteros / Lucía Beltrán-Camacho / Marta Rojas-Torres / Antonio Rosal-Vela / Margarita Jimenez-Palomares / Ismael Sanchez-Gomar / Mª Carmen Durán-Ruiz

Stem Cell Research & Therapy, Vol 14, Iss 1, Pp 1-

2023 Volume 24

Abstract: Abstract Diabetes mellitus (DM) constitutes a chronic metabolic disease characterized by elevated levels of blood glucose which can also lead to the so-called diabetic vascular complications (DVCs), responsible for most of the morbidity, hospitalizations ...

Abstract	Abstract Diabetes mellitus (DM) constitutes a chronic metabolic disease characterized by elevated levels of blood glucose which can also lead to the so-called diabetic vascular complications (DVCs), responsible for most of the morbidity, hospitalizations and death registered in these patients. Currently, different approaches to prevent or reduce DM and its DVCs have focused on reducing blood sugar levels, cholesterol management or even changes in lifestyle habits. However, even the strictest glycaemic control strategies are not always sufficient to prevent the development of DVCs, which reflects the need to identify reliable biomarkers capable of predicting further vascular complications in diabetic patients. Endothelial progenitor cells (EPCs), widely known for their potential applications in cell therapy due to their regenerative properties, may be used as differential markers in DVCs, considering that the number and functionality of these cells are affected under the pathological environments related to DM. Besides, drugs commonly used with DM patients may influence the level or behaviour of EPCs as a pleiotropic effect that could finally be decisive in the prognosis of the disease. In the current review, we have analysed the relationship between diabetes and DVCs, focusing on the potential use of EPCs as biomarkers of diabetes progression towards the development of major vascular complications. Moreover, the effects of different drugs on the number and function of EPCs have been also addressed. Graphical Abstract
Keywords	Diabetes mellitus ; Diabetic vascular complications ; Endothelial progenitor cells ; Biomarkers ; Medicine (General) ; R5-920 ; Biochemistry ; QD415-436
Subject code	610
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
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Article ; Online: Modulating Elements of Nurse Resilience in Population Care during the COVID-19 Pandemic

Ester Sierra-García / Eva María Sosa-Palanca / Carlos Saus-Ortega / Antonio Ruiz-Hontangas / Raúl Juárez-Vela / Vicente Gea-Caballero

International Journal of Environmental Research and Public Health, Vol 19, Iss 4452, p

2022 Volume 4452

Abstract	COVID-19 has significantly affected the work environment of nurses. In the face of the challenges posed by stressors in clinical practice, some nurses adapt and prove to be resilient. In the face of the COVID-19 pandemic, the nature of care itself and the new ways of working are potentially very stressful. We aim to analyze the resilience of care nurses to the psychological impact of the COVID-19 pandemic. This study is a systematic review of nurse caregiver resilience to the COVID-19 pandemic in 2021. Our search was conducted in the WOS, Medline/PubMed, Cochrane, BVS/LILACS, and Cuiden databases. The inclusion criteria were: studies published in Spanish or English; carried out from March 2020 to May 2021 on nurses caring for patients with COVID-19; and investigating the factors influencing the psychological impact, resilience, strategies to develop it, and interventions to promote it during this pandemic and others, such as SARS, MERS, or ebola. The quality of the studies and the risk of bias were evaluated following ICROMS, STROBE and AMSTAR-2 criteria. Twenty-two studies were selected. Most of the studies highlighted the presence of stressors in nurses, emphasizing those of the environment, which converged in dysfunctional responses that hurt their resilience. The most persuasive factors were social and organizational support. Coping strategies developed by nurses and especially interventions by organizations were detected as instruments to foster resilience, but have not been well researched. Resilience has a key moderating role in mitigating the psychological impact of nurses in the face of the COVID-19 pandemic.
Keywords	nurse ; COVID-19 ; resilience ; coping ; stress ; uncertainty ; Medicine ; R
Subject code	306
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Specific unsaturated fatty acids enforce the transdifferentiation of human cancer cells toward adipocyte-like cells.

Ruiz-Vela, Antonio / Aguilar-Gallardo, Cristóbal / Martínez-Arroyo, Ana M / Soriano-Navarro, Mario / Ruiz, Verónica / Simón, Carlos

Stem cell reviews and reports

2011 Volume 7, Issue 4, Page(s) 898–909

Abstract: Differentiation therapy pursues the discovery of novel molecules to transform cancer progression into less aggressive phenotypes by mechanisms involving enforced cell transdifferentiation. In this study, we examined the identification of ... ...

Abstract	Differentiation therapy pursues the discovery of novel molecules to transform cancer progression into less aggressive phenotypes by mechanisms involving enforced cell transdifferentiation. In this study, we examined the identification of transdifferentiating adipogenic programs in human cancer cell lines (HCCLs). Our findings showed that specific unsatturated fatty acids, such as palmitoleic, oleic and linoleic acids, trigger remarkable phenotypic modifications in a large number of human cancer cell lines (HCCLs), including hepatocarcinoma HUH-7, ovarian carcinoma SK-OV-3, breast adenocarcinoma MCF-7 and melanoma MALME-3M. In particular, we characterized a massive biogenesis of lipid droplets (LDs) and up-regulation of the adipogenic master regulator, PPARG, resulting in the transdifferentiation of HCCLs into adipocyte-like cells. These findings suggest the possibility of a novel strategy in cancer differentiation therapy via switching the identity of HCCLs to an adipogenic phenotype through unsaturated fatty acid-induced transdifferentiation.
MeSH term(s)	Adipocytes/cytology ; Adipogenesis ; Albumins/metabolism ; Cell Line, Tumor ; Cell Transdifferentiation ; Culture Media, Conditioned/metabolism ; Cytoplasmic Granules/metabolism ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Fatty Acids, Monounsaturated/metabolism ; Fatty Acids, Unsaturated/metabolism ; Humans ; Linoleic Acids/metabolism ; Oleic Acids/metabolism ; PPAR gamma/metabolism ; Phenotype ; Serum/metabolism
Chemical Substances	Albumins ; Culture Media, Conditioned ; Fatty Acids, Monounsaturated ; Fatty Acids, Unsaturated ; Linoleic Acids ; Oleic Acids ; PPAR gamma ; palmitoleic acid (209B6YPZ4I) ; petroselinic acid (3A10DOC461)
Language	English
Publishing date	2011-04-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2495577-2
ISSN	2629-3277 ; 1558-6804 ; 1550-8943
ISSN (online)	2629-3277 ; 1558-6804
ISSN	1550-8943
DOI	10.1007/s12015-011-9253-7
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Article ; Online: Comparison of a new Scheimpflug imaging combined with partial coherence interferometry biometer and a low-coherence reflectometry biometer.

Ruiz-Mesa, Ramón / Abengózar-Vela, Antonio / Ruiz-Santos, María

Journal of cataract and refractive surgery

2017 Volume 43, Issue 11, Page(s) 1406–1412

Abstract: Purpose: To evaluate the repeatability of a new biometer using Scheimpflug technology combined with partial coherence interferometry (PCI) (Pentacam AXL) and its agreement with a device based on optical low-coherence reflectometry (OLCR), the Allegro ... ...

Abstract	Purpose: To evaluate the repeatability of a new biometer using Scheimpflug technology combined with partial coherence interferometry (PCI) (Pentacam AXL) and its agreement with a device based on optical low-coherence reflectometry (OLCR), the Allegro Biograph. Setting: Oftalvist Centro Integral Ocular Jerez, Jerez de la Frontera, Spain. Design: Evaluation of a diagnostic test. Methods: The mean keratometry (K), central corneal thickness (CCT), anterior chamber depth (ACD), and axial length (AL) were measured with the 2 devices 3 times by the same examiner in 2 groups (patients with cataract and patients without cataract). The repeatability was determined using the within-subject standard deviation, test-retest repeatability, coefficient of variation, and intraclass correlation coefficient. The correlation was evaluated with the Pearson coefficient and interchangeability with the Bland-Altman plot. Results: Eighty eyes (40 eyes in each group) of 80 patients were analyzed. Significant differences were found between the Scheimpflug-PCI device and the OLCR device for mean K in the normal group (P < .001) and for CCT in the normal group (P < .05) and the cataract group (P < .001). There were no differences between devices in ACD and AL in either group. The repeatability between devices was similar. Although a significant correlation between devices was found for all measurements (all P < .001), wide limits of agreement were found in both groups for all biometric parameters. Conclusions: The Scheimpflug-PCI and OLCR devices showed excellent intravisit repeatability and high correlation for mean K, CCT, ACD, and AL in healthy and cataractous eyes. No differences were found in AL; however, the 2 devices might not be interchangeable.
MeSH term(s)	Anterior Chamber ; Axial Length, Eye ; Biometry ; Cataract ; Cornea/anatomy & histology ; Humans ; Interferometry/instrumentation ; Tomography, Optical Coherence
Language	English
Publishing date	2017-12-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	632744-8
ISSN	1873-4502 ; 0886-3350
ISSN (online)	1873-4502
ISSN	0886-3350
DOI	10.1016/j.jcrs.2017.08.016
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