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Article: Characterization of KLHL14 anti-oncogenic action in malignant mesothelioma.

Canciello, Angelo / Domínguez, Reyes Benot / Barboni, Barbara / Giordano, Antonio / Morrione, Andrea

2024 Volume 10, Issue 6, Page(s) e27731

Abstract: Malignant mesothelioma (MM) is a very aggressive neoplasia with a short life expectancy and limited therapeutic options. Thus, the identification of novel molecular targets is a matter of great urgency. Kelch-like (KLHL) proteins play an important role ... ...

Abstract	Malignant mesothelioma (MM) is a very aggressive neoplasia with a short life expectancy and limited therapeutic options. Thus, the identification of novel molecular targets is a matter of great urgency. Kelch-like (KLHL) proteins play an important role in a number of physiological and pathological cell-regulatory processes. Among this family, the function of KLHL14 is still very poorly characterized.
Language	English
Publishing date	2024-03-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e27731
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Stem-Cell-Driven Chondrogenesis: Perspectives on Amnion-Derived Cells.

Sulcanese, Ludovica / Prencipe, Giuseppe / Canciello, Angelo / Cerveró-Varona, Adrián / Perugini, Monia / Mauro, Annunziata / Russo, Valentina / Barboni, Barbara

Cells

2024 Volume 13, Issue 9

Abstract: Regenerative medicine harnesses stem cells' capacity to restore damaged tissues and organs. In vitro methods employing specific bioactive molecules, such as growth factors, bio-inductive scaffolds, 3D cultures, co-cultures, and mechanical stimuli, steer ... ...

Abstract	Regenerative medicine harnesses stem cells' capacity to restore damaged tissues and organs. In vitro methods employing specific bioactive molecules, such as growth factors, bio-inductive scaffolds, 3D cultures, co-cultures, and mechanical stimuli, steer stem cells toward the desired differentiation pathways, mimicking their natural development. Chondrogenesis presents a challenge for regenerative medicine. This intricate process involves precise modulation of chondro-related transcription factors and pathways, critical for generating cartilage. Cartilage damage disrupts this process, impeding proper tissue healing due to its unique mechanical and anatomical characteristics. Consequently, the resultant tissue often forms fibrocartilage, which lacks adequate mechanical properties, posing a significant hurdle for effective regeneration. This review comprehensively explores studies showcasing the potential of amniotic mesenchymal stem cells (AMSCs) and amniotic epithelial cells (AECs) in chondrogenic differentiation. These cells exhibit innate characteristics that position them as promising candidates for regenerative medicine. Their capacity to differentiate toward chondrocytes offers a pathway for developing effective regenerative protocols. Understanding and leveraging the innate properties of AMSCs and AECs hold promise in addressing the challenges associated with cartilage repair, potentially offering superior outcomes in tissue regeneration.
MeSH term(s)	Humans ; Chondrogenesis ; Amnion/cytology ; Cell Differentiation ; Animals ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Chondrocytes/cytology ; Chondrocytes/metabolism ; Regenerative Medicine/methods ; Tissue Engineering/methods
Language	English
Publishing date	2024-04-24
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells13090744
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Article ; Online: Amniotic Membrane and Amniotic Epithelial Cell Culture.

Canciello, Angelo / Cerverò-Varona, Adrian / Turriani, Maura / Russo, Valentina / Barboni, Barbara

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2749, Page(s) 135–149

Abstract: Amniotic membrane (AM) is considered an important medical device for applications in regenerative medicine. The therapeutic properties of AM are due to its resistant extracellular matrix and to the large number of bioactive molecules released by its ... ...

Abstract	Amniotic membrane (AM) is considered an important medical device for applications in regenerative medicine. The therapeutic properties of AM are due to its resistant extracellular matrix and to the large number of bioactive molecules released by its cells. To this regard, ovine amniotic epithelial cells (AECs) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, either oAEC or AM have been object of intense study for regenerative medicine, thanks to several advantages in developing preclinical studies on a high value translational animal model, such as sheep. For this reason, a critical standardization of cultural practices is fundamental in order to maintain, on one hand, AM integrity and structure and, on the other hand, oAEC native properties, thus improving their in vivo therapeutic potential and clinical outcomes.In addition, freshly isolated AECs or AM can be exploited to produce enriched immunomodulatory secretomes that had been used with success into cell-free regenerative medicine procedures.To this aim, here is described an improved oAEC cultural protocol able to preserve their native epithelial phenotype also after the in vitro amplification and an innovative AM in vitro cultural protocol design to prolong the integrity and the biological properties of this tissue in order to collect stable conditioned media enriched with immunomodulatory factors.
MeSH term(s)	Sheep ; Animals ; Female ; Pregnancy ; Amnion ; Placenta ; Cell Culture Techniques ; Epithelial Cells ; Regenerative Medicine
Language	English
Publishing date	2023-12-22
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3609-1_13
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Article: "In medio stat virtus"

Canciello, Angelo / Cerveró-Varona, Adrián / Peserico, Alessia / Mauro, Annunziata / Russo, Valentina / Morrione, Andrea / Giordano, Antonio / Barboni, Barbara

Frontiers in cell and developmental biology

2022 Volume 10, Page(s) 1038841

Abstract: Epithelial-mesenchymal plasticity (EMP) refers to the ability of cells to dynamically interconvert between epithelial (E) and mesenchymal (M) phenotypes, thus generating an array of hybrid E/M intermediates with mixed E and M features. Recent findings ... ...

Abstract	Epithelial-mesenchymal plasticity (EMP) refers to the ability of cells to dynamically interconvert between epithelial (E) and mesenchymal (M) phenotypes, thus generating an array of hybrid E/M intermediates with mixed E and M features. Recent findings have demonstrated how these hybrid E/M rather than fully M cells play key roles in most of physiological and pathological processes involving EMT. To this regard, the onset of hybrid E/M state coincides with the highest stemness gene expression and is involved in differentiation of either normal and cancer stem cells. Moreover, hybrid E/M cells are responsible for wound healing and create a favorable immunosuppressive environment for tissue regeneration. Nevertheless, hybrid state is responsible of metastatic process and of the increasing of survival, apoptosis and therapy resistance in cancer cells. The present review aims to describe the main features and the emerging concepts regulating EMP and the formation of E/M hybrid intermediates by describing differences and similarities between cancer and normal hybrid stem cells. In particular, the comprehension of hybrid E/M cells biology will surely advance our understanding of their features and how they could be exploited to improve tissue regeneration and repair.
Language	English
Publishing date	2022-11-18
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2737824-X
ISSN	2296-634X
ISSN	2296-634X
DOI	10.3389/fcell.2022.1038841
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Article: Nanotechnology-Assisted Cell Tracking.

Peserico, Alessia / Di Berardino, Chiara / Russo, Valentina / Capacchietti, Giulia / Di Giacinto, Oriana / Canciello, Angelo / Camerano Spelta Rapini, Chiara / Barboni, Barbara

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 9

Abstract: The usefulness of nanoparticles (NPs) in the diagnostic and/or therapeutic sector is derived from their aptitude for navigating intra- and extracellular barriers successfully and to be spatiotemporally targeted. In this context, the optimization of NP ... ...

Abstract	The usefulness of nanoparticles (NPs) in the diagnostic and/or therapeutic sector is derived from their aptitude for navigating intra- and extracellular barriers successfully and to be spatiotemporally targeted. In this context, the optimization of NP delivery platforms is technologically related to the exploitation of the mechanisms involved in the NP-cell interaction. This review provides a detailed overview of the available technologies focusing on cell-NP interaction/detection by describing their applications in the fields of cancer and regenerative medicine. Specifically, a literature survey has been performed to analyze the key nanocarrier-impacting elements, such as NP typology and functionalization, the ability to tune cell interaction mechanisms under in vitro and in vivo conditions by framing, and at the same time, the imaging devices supporting NP delivery assessment, and consideration of their specificity and sensitivity. Although the large amount of literature information on the designs and applications of cell membrane-coated NPs has reached the extent at which it could be considered a mature branch of nanomedicine ready to be translated to the clinic, the technology applied to the biomimetic functionalization strategy of the design of NPs for directing cell labelling and intracellular retention appears less advanced. These approaches, if properly scaled up, will present diverse biomedical applications and make a positive impact on human health.
Language	English
Publishing date	2022-04-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12091414
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Article ; Online: Amniotic Epithelial Cell Culture.

Canciello, Angelo / Greco, Luana / Russo, Valentina / Barboni, Barbara

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1817, Page(s) 67–78

Abstract: Ovine amniotic epithelial cells (oAEC) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, oAEC are object of intense study for regenerative medicine thanks to the several advantages in developing pre- ... ...

Abstract	Ovine amniotic epithelial cells (oAEC) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, oAEC are object of intense study for regenerative medicine thanks to the several advantages in developing pre-clinical studies on a high value translational animal model, such as sheep. For this reason, a critical standardization of cultural practices is fundamental to preserve in vitro oAEC native phenotype, thus improving their in vivo therapeutic potential and clinical outcomes. Here is described an improved oAEC cultural protocol able to preserve the native epithelial phenotype also after the in vitro amplification.
MeSH term(s)	Amnion/cytology ; Animals ; Cell Culture Techniques/standards ; Cell Culture Techniques/veterinary ; Cell Differentiation ; Cell Proliferation/drug effects ; Cells, Cultured ; Culture Media/chemistry ; Epithelial Cells/cytology ; Epithelial-Mesenchymal Transition ; Female ; Phenotype ; Pregnancy ; Progesterone/pharmacology ; Sheep
Chemical Substances	Culture Media ; Progesterone (4G7DS2Q64Y)
Language	English
Publishing date	2018-07-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-8600-2_7
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Article ; Online: Graphene oxide accelerates TGFβ-mediated epithelial-mesenchymal transition and stimulates pro-inflammatory immune response in amniotic epithelial cells.

Cerverò-Varona, Adrian / Canciello, Angelo / Peserico, Alessia / Haidar Montes, Arlette Alina / Citeroni, Maria Rita / Mauro, Annunziata / Russo, Valentina / Moffa, Samanta / Pilato, Serena / Di Giacomo, Stefano / Dufrusine, Beatrice / Dainese, Enrico / Fontana, Antonella / Barboni, Barbara

Materials today. Bio

2023 Volume 22, Page(s) 100758

Abstract: The application of biomaterials on immune regenerative strategies to deal with unsolved pathologies is getting attention in the field of tissue engineering. In this context, graphene oxide (GO) has been proposed as an immune-mimetic material largely used ...

Abstract	The application of biomaterials on immune regenerative strategies to deal with unsolved pathologies is getting attention in the field of tissue engineering. In this context, graphene oxide (GO) has been proposed as an immune-mimetic material largely used for developing stem cell-based regenerative therapies, since it has shown to influence stem cell behavior and modulate their immune response. Similarly, amniotic epithelial stem cells (AECs) are getting an increasing clinical interest as source of stem cells due to their great plasticity and immunomodulatory paracrine activities, even though GO bio-mimetic effects still remain unknown. To this aim, GO-functionalized glass coverslips have been used for AECs culture. The results demonstrated how GO-coating is able to induce and accelerate the Epithelial-Mesenchymal Transition (EMT), in a process mediated by the intracellular activation of TGFβ1-SMAD2/3 signaling pathway. The
Language	English
Publishing date	2023-08-02
Publishing country	England
Document type	Journal Article
ISSN	2590-0064
ISSN (online)	2590-0064
DOI	10.1016/j.mtbio.2023.100758
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Article: Progesterone Prolongs Viability and Anti-inflammatory Functions of Explanted Preterm Ovine Amniotic Membrane.

Canciello, Angelo / Teti, Gabriella / Mazzotti, Eleonora / Falconi, Mirella / Russo, Valentina / Giordano, Antonio / Barboni, Barbara

Frontiers in bioengineering and biotechnology

2020 Volume 8, Page(s) 135

Abstract: Amniotic membrane (AM) is considered an important medical device with many applications in regenerative medicine. The therapeutic properties of AM are due to its resistant extracellular matrix and to the large number of bioactive molecules released by ... ...

Abstract	Amniotic membrane (AM) is considered an important medical device with many applications in regenerative medicine. The therapeutic properties of AM are due to its resistant extracellular matrix and to the large number of bioactive molecules released by its cells. An important goal that still remains to be achieved is the identification of cultural and preservation protocols able to maintain in time the membrane morphology and the biological properties of its cells. Recently, our research group demonstrated that progesterone (P
Language	English
Publishing date	2020-03-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2020.00135
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Article ; Online: “In medio stat virtus”

Angelo Canciello / Adrián Cerveró-Varona / Alessia Peserico / Annunziata Mauro / Valentina Russo / Andrea Morrione / Antonio Giordano / Barbara Barboni

Frontiers in Cell and Developmental Biology, Vol

Insights into hybrid E/M phenotype attitudes

2022 Volume 10

Abstract	Epithelial-mesenchymal plasticity (EMP) refers to the ability of cells to dynamically interconvert between epithelial (E) and mesenchymal (M) phenotypes, thus generating an array of hybrid E/M intermediates with mixed E and M features. Recent findings have demonstrated how these hybrid E/M rather than fully M cells play key roles in most of physiological and pathological processes involving EMT. To this regard, the onset of hybrid E/M state coincides with the highest stemness gene expression and is involved in differentiation of either normal and cancer stem cells. Moreover, hybrid E/M cells are responsible for wound healing and create a favorable immunosuppressive environment for tissue regeneration. Nevertheless, hybrid state is responsible of metastatic process and of the increasing of survival, apoptosis and therapy resistance in cancer cells. The present review aims to describe the main features and the emerging concepts regulating EMP and the formation of E/M hybrid intermediates by describing differences and similarities between cancer and normal hybrid stem cells. In particular, the comprehension of hybrid E/M cells biology will surely advance our understanding of their features and how they could be exploited to improve tissue regeneration and repair.
Keywords	hybrid E/M phenotype ; partial EMT ; stem cell ; cancer ; immune evasion ; immune suppression ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Nanotechnology-Assisted Cell Tracking

Alessia Peserico / Chiara Di Berardino / Valentina Russo / Giulia Capacchietti / Oriana Di Giacinto / Angelo Canciello / Chiara Camerano Spelta Rapini / Barbara Barboni

Nanomaterials, Vol 12, Iss 1414, p

2022 Volume 1414

Abstract	The usefulness of nanoparticles (NPs) in the diagnostic and/or therapeutic sector is derived from their aptitude for navigating intra- and extracellular barriers successfully and to be spatiotemporally targeted. In this context, the optimization of NP delivery platforms is technologically related to the exploitation of the mechanisms involved in the NP–cell interaction. This review provides a detailed overview of the available technologies focusing on cell–NP interaction/detection by describing their applications in the fields of cancer and regenerative medicine. Specifically, a literature survey has been performed to analyze the key nanocarrier-impacting elements, such as NP typology and functionalization, the ability to tune cell interaction mechanisms under in vitro and in vivo conditions by framing, and at the same time, the imaging devices supporting NP delivery assessment, and consideration of their specificity and sensitivity. Although the large amount of literature information on the designs and applications of cell membrane-coated NPs has reached the extent at which it could be considered a mature branch of nanomedicine ready to be translated to the clinic, the technology applied to the biomimetic functionalization strategy of the design of NPs for directing cell labelling and intracellular retention appears less advanced. These approaches, if properly scaled up, will present diverse biomedical applications and make a positive impact on human health.
Keywords	nanoparticles ; cell tracking ; cell labelling ; cell uptake ; stem cell homing ; Chemistry ; QD1-999
Subject code	500
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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