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Article ; Online: Babao Dan Alleviates Cancer Cachexia in Mice Via Inhibiting IL-6/STAT3 Signaling Pathway.

Qiu, Zhengliang / Wo, Da / Zhong, Xiaomei / Chen, Jinxiao / Ma, En / He, Jia / Peng, Jun / Zhu, Weidong / Ren, Dan-Ni

2023 Volume 22, Page(s) 15347354231168369

Abstract: ... therapeutics that can effectively prevent or even reverse cancer cachexia is crucial. Babao Dan (BBD) is ...

Abstract	Background: Cancer cachexia is a common but severe condition that causes muscle wasting, body weight loss, and progressive functional impairment, affecting over 50% of cancer patients. Currently, there are no effective treatments that can alleviate cachexia, and hence the discovery of new therapeutics that can effectively prevent or even reverse cancer cachexia is crucial. Babao Dan (BBD) is a Traditional Chinese Medicine (TCM) formula that has been used clinically in combating various cancers, however, its therapeutic potential in alleviating cancer cachexia remains unexplored. Our current study aims to determine the anti-cachectic effects of BBD treatment in alleviating cancer cachexia, as well as determining the underlying mechanisms involved. Methods: Mouse models of cancer cachexia were induced via implantation of CT26 colon adenocarcinoma cells, and the anti-cachectic effects and mechanisms of BBD were determined via examinations of body weight and muscle mass, as well as serum and muscle markers of cachexia and muscle atrophy. Results: CT26 tumor implantation reduced in the rapid occurrence of cancer cachexia characterized by marked reductions in body weight and muscle mass, functional decrease in muscle function and accelerated deaths. BBD administration not only demonstrated robust anti-cachectic ability via preventing decreases in body weight, muscle mass, and muscle atrophy, but also markedly prolonged survival. The effects of BBD in alleviating cancer cachexia and its associated adverse effects were due to its ability in preventing the activation of IL-6/STAT3 signaling post-CT26 tumor implantation. Conclusion: Our findings demonstrated the robust ability of BBD in preventing cancer cachexia and alleviating the main cachexia-induced symptoms as well as prolonging survival via inhibiting activation of IL-6/STAT3 signaling pathway. Therefore, our study demonstrating the strong anti-cachectic effects of BBD in mice may provide a theoretical basis for the use of BBD as a safe and effective drug in the treatment of cancer cachexia.
MeSH term(s)	Mice ; Animals ; Cachexia/drug therapy ; Cachexia/etiology ; Cachexia/metabolism ; Interleukin-6 ; Adenocarcinoma/drug therapy ; Colonic Neoplasms/complications ; Colonic Neoplasms/drug therapy ; Muscular Atrophy ; Signal Transduction ; Body Weight
Chemical Substances	babao dan ; Interleukin-6
Language	English
Publishing date	2023-04-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2182320-0
ISSN	1552-695X ; 1534-7354
ISSN (online)	1552-695X
ISSN	1534-7354
DOI	10.1177/15347354231168369
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Article ; Online: The effect of Ding-kun-dan comparing with Marvelon on primary dysmenorrhea: A prospective, double-blind, multicenter, randomized controlled trial.

Gan, Jingwen / Zhu, Shi-Yang / Ma, Xiao / Ding, Xue-Song / Deng, Yan / Wang, Yanfang / Sun, Ai-Jun

Journal of ethnopharmacology

2023 Volume 318, Issue Pt B, Page(s) 116975

Abstract: Ethnopharmacological relevance: Ding-kun-dan (DKD), as one of well-known ... Aim of the study: To compare the effect of Ding-kun-dan (DKD) with Marvelon on relief of symptoms and ... 4: Conclusion: Ding-kun-dan is another available, effective and safe method for patients ...

Abstract	Ethnopharmacological relevance: Ding-kun-dan (DKD), as one of well-known traditional Chinese medicine (TCM), is considered as an effective prescription to regulate menstruation, benefit Qi and nourish the blood. Previous studies had showed that DKD could improve sex hormone levels, insulin resistance, metabolism abnormalities and regulate immunity in animal models with polycystic ovary syndrome or endometriosis, however, little study conducted to reveal its clinical efficacy in Primary Dysmenorrhea (PD). Aim of the study: To compare the effect of Ding-kun-dan (DKD) with Marvelon on relief of symptoms and change of serum pain-related factors in patients with primary dysmenorrhea. Materials and method: 136 patients with primary dysmenorrhea were randomly assigned to the DKD group (n = 73, take one tablet per day from 5th day of the menstrual cycle for 10 days every 28 days) and the Marvelon group (n = 63, take one tablet per day from 5th day of the menstrual cycle for 21 days every 28 days), the therapeutic effects were analyzed through evaluating the change of VAS scores, CMSS scores and the level of PGF Results: Both DKD and Marvelon could effectively relief pain and other associated symptoms at each visit (Baseline, 4 Conclusion: Ding-kun-dan is another available, effective and safe method for patients with primary dysmenorrhea to choose, especially for those who are suffered from mild pain and/or contraindicated to hormonal agents.
MeSH term(s)	Female ; Humans ; Desogestrel/therapeutic use ; Dinoprostone ; Double-Blind Method ; Dysmenorrhea/drug therapy ; Dysmenorrhea/diagnosis ; Endometriosis ; Medicine, Chinese Traditional ; Prospective Studies
Chemical Substances	Desogestrel (81K9V7M3A3) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2023-07-29
Publishing country	Ireland
Document type	Journal Article ; Multicenter Study ; Randomized Controlled Trial
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116975
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Article: Effect of Dan-Lou tablets on coronary heart disease revealed by microarray analysis integrated with molecular mechanism studies.

Li, Zhu / Cheng, Qi / He, Yuanyuan / Wang, Shuo / Xie, Jing / Zheng, Yanchao / Liu, Yijia / Li, Lin / Gao, Shan / Yu, Chunquan

Heliyon

2023 Volume 9, Issue 5, Page(s) e15777

Abstract: Dan-Lou tablets (DLT) effectively treat coronary heart disease (CHD). However, its pharmacological ...

Abstract	Dan-Lou tablets (DLT) effectively treat coronary heart disease (CHD). However, its pharmacological mechanism in CHD treatment requires further investigation. This study aimed to elucidate the underlying pharmacological mechanisms of DLT in the treatment of CHD through clinical trials, microarray research, bioinformatics analysis, and molecular mechanism research. In this study, DLT improved coagulation function, endothelial injury, and levels of lipids, metalloproteases, adhesion molecules, inflammatory mediators, and homocysteine. The results of molecular biology research demonstrated that DLT can increase the gene and protein expressions of meningioma expressed antigen 5 (MGEA5) and mouse doubleminute 2 (MDM2) and inhibited the gene and protein expressions of signal transcription and transcription activator 5 B (STAT5B), tropomyosin-1 (TPM1), and aromatic hydrocarbon receptor nuclear transpose (ARNT). The results indicate that DLT reduced the extent of vascular endothelial damage in CHD rats by reducing the expressions of STAT5B, TPM1, and MDM2; inhibiting the inflammatory reaction; and increasing the expressions of ARNT and MGEA5.
Language	English
Publishing date	2023-04-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e15777
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Babao Dan is a robust anti-tumor agent via inhibiting wnt/β-catenin activation and cancer cell stemness.

Xie, Xinxin / Chen, Jinxiao / Wo, Da / Ma, En / Ning, Yongling / Peng, Jun / Zhu, Weidong / Ren, Dan-Ni

Journal of ethnopharmacology

2021 Volume 280, Page(s) 114449

Abstract: ... worldwide due to its diverse efficacy and relatively low side effects. Babao Dan (BBD) is a well-known TCM ... molecular mechanisms associated with the anti-tumor proliferative effects of Babao Dan (BBD).: Materials ...

Abstract	Ethnopharmacological relevance: Traditional Chinese Medicine (TCM) is being increasingly used worldwide due to its diverse efficacy and relatively low side effects. Babao Dan (BBD) is a well-known TCM formula that is currently used for the effective treatment of various cancers, however its underlying molecular mechanism remains unknown. Aim of the study: Tumor growth and tumor recurrence are characterized by two distinct populations of cells, namely the well-differentiated cancer cells composing the majority of tumor bulk, and cancer stem cells (CSCs) involved in tumor relapse, which are both strongly associated with excessive activation of Wnt/β-catenin signaling. Our study aims to elucidate the underlying molecular mechanisms associated with the anti-tumor proliferative effects of Babao Dan (BBD). Materials and methods: We used a hepatoblastoma cell line HepG2 with stem cell-like traits that harbors a constitutively active mutant of β-catenin in order to study the anti-tumor ability of BBD via targeting Wnt/β-catenin signaling. Results: BBD robustly attenuated both the intrinsic and extrinsic activation of Wnt/β-catenin pathway in HepG2 hepatoblastoma cells, as well as Wnt target genes. Moreover, BBD significantly inhibited both the proliferation of well-differentiated cancer cells, as well as the stem-like property of CSCs as evidenced by EpCAM, a Wnt target gene and a novel marker of cancer cell stemness. In addition, mice administered with BBD using HepG2 cell line derived xenograft model had marked reductions in tumor size and weight, as well as significantly decreased expressions of Wnt target genes and cancer cell stemness. Conclusion: Our findings elucidated the underlying molecular mechanisms associated with the robust anti-tumor effects of BBD via potent inhibition of Wnt/β-catenin signaling, and implicate its use in the clinical treatment of cancers.
MeSH term(s)	Animals ; Antineoplastic Agents, Phytogenic/pharmacology ; Cell Proliferation/drug effects ; Drugs, Chinese Herbal/pharmacology ; Hep G2 Cells ; Hepatoblastoma/drug therapy ; Hepatoblastoma/genetics ; Hepatoblastoma/pathology ; Humans ; Liver Neoplasms/drug therapy ; Liver Neoplasms/genetics ; Liver Neoplasms/pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Mutation ; Neoplastic Stem Cells/drug effects ; Wnt Signaling Pathway/drug effects ; Xenograft Model Antitumor Assays ; beta Catenin/genetics
Chemical Substances	Antineoplastic Agents, Phytogenic ; CTNNB1 protein, human ; Drugs, Chinese Herbal ; babao dan ; beta Catenin
Language	English
Publishing date	2021-07-28
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114449
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Article ; Online: The effect of Ding-kun-dan comparing with Marvelon on primary dysmenorrhea: A prospective, double-blind, multicenter, randomized controlled trial

Gan, Jingwen / Zhu, Shi-yang / Ma, Xiao / Ding, Xue-song / Deng, Yan / Wang, Yanfang / Sun, Ai-jun

Journal of Ethnopharmacology. 2024 Jan., v. 318 p.116975-

2024

Abstract: Ding-kun-dan (DKD), as one of well-known traditional Chinese medicine (TCM), is considered ... dan (DKD) with Marvelon on relief of symptoms and change of serum pain-related factors in patients ... kun-dan is another available, effective and safe method for patients with primary dysmenorrhea ...

Abstract	Ding-kun-dan (DKD), as one of well-known traditional Chinese medicine (TCM), is considered as an effective prescription to regulate menstruation, benefit Qi and nourish the blood. Previous studies had showed that DKD could improve sex hormone levels, insulin resistance, metabolism abnormalities and regulate immunity in animal models with polycystic ovary syndrome or endometriosis, however, little study conducted to reveal its clinical efficacy in Primary Dysmenorrhea (PD). To compare the effect of Ding-kun-dan (DKD) with Marvelon on relief of symptoms and change of serum pain-related factors in patients with primary dysmenorrhea. 136 patients with primary dysmenorrhea were randomly assigned to the DKD group (n = 73, take one tablet per day from 5th day of the menstrual cycle for 10 days every 28 days) and the Marvelon group (n = 63, take one tablet per day from 5th day of the menstrual cycle for 21 days every 28 days), the therapeutic effects were analyzed through evaluating the change of VAS scores, CMSS scores and the level of PGF₂ₐ, PGE₂, PGF₂ₐ/PGE₂ and NO during the 12 weeks intervention. Both DKD and Marvelon could effectively relief pain and other associated symptoms at each visit (Baseline, 4ᵗʰ week, 8ᵗʰ week and 12ᵗʰ week). Although Marvelon was significantly superior to DKD in reducing VAS scores, the total CMSS, CMSS severity and duration scores at each posttreatment follow-up (P < 0.01), VAS scores in the DKD group decreased significantly over time while scores in the COC group only dropped rapidly after the first two months of treatment. Efficacy gap between two interventions continuously narrowed over time and the efficacy of DKD became non-inferior at the 12ᵗʰ week compared to that of Marvelon (the difference between groups, – 0.78%; 95% confidence interval (CI), −13.67%–12.75%; non-inferiority margin, 15%). DKD group had better efficacy on mild pain compared to that of the COC group with no statistical difference (75% VS 61.9%, P > 0.05). DKD and Marvelon could effectively reduce PGF₂ₐ, PGE₂ and higher PGF₂ₐ/PGE₂ in patients with PD. There was no statistical difference in the level of PGF₂ₐ, PGE₂, PGF₂ₐ/PGE₂ and NO between DKD and Marvelon group at each follow-up. No serious adverse effect was observed. Ding-kun-dan is another available, effective and safe method for patients with primary dysmenorrhea to choose, especially for those who are suffered from mild pain and/or contraindicated to hormonal agents.
Keywords	Oriental traditional medicine ; adverse effects ; animals ; blood serum ; confidence interval ; endometriosis ; immunity ; insulin resistance ; menstruation ; metabolism ; pain ; polycystic ovary syndrome ; therapeutics ; Primary dysmenorrhea ; Ding-kun-dan ; Marvelon ; VAS ; CMSS ; PGF2a ; PGE2 ; PGF2a/PGE2
Language	English
Dates of publication	2024-01
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116975
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Effect of Dan-Lou tablets on coronary heart disease revealed by microarray analysis integrated with molecular mechanism studies

Zhu Li / Qi Cheng / Yuanyuan He / Shuo Wang / Jing Xie / Yanchao Zheng / Yijia Liu / Lin Li / Shan Gao / Chunquan Yu

Heliyon, Vol 9, Iss 5, Pp e15777- (2023)

2023

Abstract: Dan-Lou tablets (DLT) effectively treat coronary heart disease (CHD). However, its pharmacological ...

Abstract	Dan-Lou tablets (DLT) effectively treat coronary heart disease (CHD). However, its pharmacological mechanism in CHD treatment requires further investigation. This study aimed to elucidate the underlying pharmacological mechanisms of DLT in the treatment of CHD through clinical trials, microarray research, bioinformatics analysis, and molecular mechanism research. In this study, DLT improved coagulation function, endothelial injury, and levels of lipids, metalloproteases, adhesion molecules, inflammatory mediators, and homocysteine. The results of molecular biology research demonstrated that DLT can increase the gene and protein expressions of meningioma expressed antigen 5 (MGEA5) and mouse doubleminute 2 (MDM2) and inhibited the gene and protein expressions of signal transcription and transcription activator 5 B (STAT5B), tropomyosin-1 (TPM1), and aromatic hydrocarbon receptor nuclear transpose (ARNT). The results indicate that DLT reduced the extent of vascular endothelial damage in CHD rats by reducing the expressions of STAT5B, TPM1, and MDM2; inhibiting the inflammatory reaction; and increasing the expressions of ARNT and MGEA5.
Keywords	Dan-Lou tablets ; Coronary heart disease ; Affymetrix ; Molecular docking ; Molecular biology ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	610
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Dan-Lou tablets on coronary heart disease revealed by microarray analysis integrated with molecular mechanism studies

Li, Zhu / Cheng, Qi / He, Yuanyuan / Wang, Shuo / Xie, Jing / Zheng, Yanchao / Liu, Yijia / Li, Lin / Gao, Shan / Yu, Chunquan

Heliyon. 2023 May, v. 9, no. 5 p.e15777-

2023

Abstract: Dan-Lou tablets (DLT) effectively treat coronary heart disease (CHD). However, its pharmacological ...

Abstract	Dan-Lou tablets (DLT) effectively treat coronary heart disease (CHD). However, its pharmacological mechanism in CHD treatment requires further investigation. This study aimed to elucidate the underlying pharmacological mechanisms of DLT in the treatment of CHD through clinical trials, microarray research, bioinformatics analysis, and molecular mechanism research. In this study, DLT improved coagulation function, endothelial injury, and levels of lipids, metalloproteases, adhesion molecules, inflammatory mediators, and homocysteine. The results of molecular biology research demonstrated that DLT can increase the gene and protein expressions of meningioma expressed antigen 5 (MGEA5) and mouse doubleminute 2 (MDM2) and inhibited the gene and protein expressions of signal transcription and transcription activator 5 B (STAT5B), tropomyosin-1 (TPM1), and aromatic hydrocarbon receptor nuclear transpose (ARNT). The results indicate that DLT reduced the extent of vascular endothelial damage in CHD rats by reducing the expressions of STAT5B, TPM1, and MDM2; inhibiting the inflammatory reaction; and increasing the expressions of ARNT and MGEA5.
Keywords	adhesion ; antigens ; bioinformatics ; coagulation ; coronary disease ; genes ; homocysteine ; metalloproteinases ; microarray technology ; molecular biology ; Dan-Lou tablets ; Coronary heart disease ; Affymetrix ; Molecular docking ; PT ; TT ; APTT ; INR ; FIB ; TC ; TG ; LDL-C ; HDL-C ; ET-1 ; ANG-I ; MMP2 ; ICAM-1 ; NF-κB ; GM-CSF ; sCD40L ; IL-1 ; IL-6 ; MCP-1 ; TNF-α ; MGEA5 ; ARNT ; STAT5B ; TPM1 ; MDM2
Language	English
Dates of publication	2023-05
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e15777
Database	NAL-Catalogue (AGRICOLA)

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Article: Babao Dan is a robust anti-tumor agent via inhibiting wnt/β-catenin activation and cancer cell stemness

Xie, Xinxin / Chen, Jinxiao / Wo, Da / Ma, En / Ning, Yongling / Peng, Jun / Zhu, Weidong / Ren, Dan-ni

Journal of ethnopharmacology. 2021 Nov. 15, v. 280

2021

Abstract: ... and relatively low side effects. Babao Dan (BBD) is a well-known TCM formula that is currently used ... effects of Babao Dan (BBD).We used a hepatoblastoma cell line HepG2 with stem cell-like traits ...

Abstract	Traditional Chinese Medicine (TCM) is being increasingly used worldwide due to its diverse efficacy and relatively low side effects. Babao Dan (BBD) is a well-known TCM formula that is currently used for the effective treatment of various cancers, however its underlying molecular mechanism remains unknown.Tumor growth and tumor recurrence are characterized by two distinct populations of cells, namely the well-differentiated cancer cells composing the majority of tumor bulk, and cancer stem cells (CSCs) involved in tumor relapse, which are both strongly associated with excessive activation of Wnt/β-catenin signaling. Our study aims to elucidate the underlying molecular mechanisms associated with the anti-tumor proliferative effects of Babao Dan (BBD).We used a hepatoblastoma cell line HepG2 with stem cell-like traits that harbors a constitutively active mutant of β-catenin in order to study the anti-tumor ability of BBD via targeting Wnt/β-catenin signaling.BBD robustly attenuated both the intrinsic and extrinsic activation of Wnt/β-catenin pathway in HepG2 hepatoblastoma cells, as well as Wnt target genes. Moreover, BBD significantly inhibited both the proliferation of well-differentiated cancer cells, as well as the stem-like property of CSCs as evidenced by EpCAM, a Wnt target gene and a novel marker of cancer cell stemness. In addition, mice administered with BBD using HepG2 cell line derived xenograft model had marked reductions in tumor size and weight, as well as significantly decreased expressions of Wnt target genes and cancer cell stemness.Our findings elucidated the underlying molecular mechanisms associated with the robust anti-tumor effects of BBD via potent inhibition of Wnt/β-catenin signaling, and implicate its use in the clinical treatment of cancers.
Keywords	Oriental traditional medicine ; antineoplastic agents ; genes ; human cell lines ; models ; mutants ; neoplasm cells ; neoplasms ; relapse ; xenotransplantation
Language	English
Dates of publication	2021-1115
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2021.114449
Database	NAL-Catalogue (AGRICOLA)

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Article: Kun-Dan Decoction Ameliorates Insulin Resistance by Activating AMPK/mTOR-Mediated Autophagy in High-Fat Diet-Fed Rats.

Su, Zuqing / Zeng, Kexue / Feng, Bing / Tang, Lipeng / Sun, Chaoyue / Wang, Xieqi / Li, Caiyun / Zheng, Guangjuan / Zhu, Ying

Frontiers in pharmacology

2021 Volume 12, Page(s) 670151

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2021-05-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.670151
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The effect and mechanism of Qingre Huashi formula in the treatment of chronic hepatitis B with Gan-dan-shi-Re syndrome: An integrated transcriptomic and targeted metabolomic analysis.

Lu, Yiyu / Zhao, Chaoqun / Wang, Chengbao / Cai, Hong / Hu, Yuting / Chen, Long / Yu, Shanghai / Zhu, Huiming / Liu, Ping / Wan'e, Wang / Zhang, Hua

Journal of ethnopharmacology

2023 Volume 319, Issue Pt 1, Page(s) 117092

Abstract: ... for the treatment of Gan-Dan-Shi-Re syndrome (GDSR) syndrome in traditional Chinese medicine (TCM). GDSR is one ...

Abstract	Ethnopharmacological relevance: Qingre Huashi (QRHS) formula is an empirical prescription for the treatment of Gan-Dan-Shi-Re syndrome (GDSR) syndrome in traditional Chinese medicine (TCM). GDSR is one of the typical TCM syndromes in chronic hepatitis B (CHB). However, little is known about the mechanism of the QRHS formula in treating CHB patients with GDSR. The biological basis of GDSR also remains largely unknown. Aim of the study: GDSR mostly occurs in the acute and early stages of chronic liver disease. Effectively alleviating GDSR stalls disease development and benefits patients. The purpose of this study was to explore the molecular basis of GDSR in CHB and then study the mechanism of the QRHS formula treating GDSR using transcriptomics and metabolomics. Materials and methods: The transcriptome and metabolome of CHB patients with GDSR syndrome were detected using RNA microarray combined with ultra-high performance liquid chromatography/mass spectrometry and information mining. The potential biomarkers were identified from differentially expressed genes and metabolites, and the metabolic pathway was analyzed. We also investigated the callback of metabolic biomarkers after treatment with the QRHS formula, an empirical prescription for the treatment of GDSR syndrome. RT-PCR analysis was carried out in an independent patient cohort of CHB for validation. Results: Four candidate genes-GPT2, HK2, DDIT3, and HIF1A-and 14 candidate metabolic biomarkers, including L-alpha-aminobutyric acid, selenomethionine, and fructose 1,6-bisphosphate, were identified and validated. All four transcripts of GPT2, HK2, DDIT3, and HIF1A were significantly differentially expressed between the GDSR and non-GDSR groups through independent microarray data and RT-PCR. After treatment with the QRHS formula, the clinical indexes and TCM syndrome were significantly improved, and the 14 disturbed biomarkers were obviously corrected. Three metabolic pathways were confirmed to be perturbed in CHB GDSR patients: alanine, aspartate, and glutamate metabolism, arginine biosynthesis, and aminoacyl-tRNA biosynthesis. Conclusion: Using integrated transcriptomic and targeted metabolomic methods, we identified the potential biomarkers and dysregulated metabolic pathways in CHB patients with GDSR syndrome, which was alleviated by the QRHS formula treatment. These results may provide the mechanism of metabolic dysregulation in GDSR syndrome as well as that underlying the curative effect of the QRHS formula.
MeSH term(s)	Humans ; Transcriptome ; Hepatitis B, Chronic/drug therapy ; Hepatitis B, Chronic/genetics ; Metabolomics/methods ; Medicine, Chinese Traditional/methods ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Drugs, Chinese Herbal/metabolism ; Biomarkers
Chemical Substances	Drugs, Chinese Herbal ; Biomarkers
Language	English
Publishing date	2023-08-25
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117092
Database	MEDical Literature Analysis and Retrieval System OnLINE

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