LIVIVO - Search results -

Search results

Result 1 - 10 of total 70

Search options

Article ; Online: Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance.

Muñoz-Gallego, Irene / Meléndez Carmona, María Ángeles / Martín Higuera, Carmen / Viedma, Esther / Delgado, Rafael / Folgueira, María Dolores

Scientific reports

2023 Volume 13, Issue 1, Page(s) 11094

Abstract: The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout ... ...

Abstract	The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.
MeSH term(s)	Humans ; SARS-CoV-2/genetics ; COVID-19/diagnosis ; COVID-19/epidemiology ; Pandemics ; Laboratories
Language	English
Publishing date	2023-07-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-37866-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance

Irene Muñoz-Gallego / María Ángeles Meléndez Carmona / Carmen Martín Higuera / Esther Viedma / Rafael Delgado / María Dolores Folgueira

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 5

Abstract: Abstract The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) ... ...

Abstract	Abstract The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19.

Folgueira, Maria Dolores / Luczkowiak, Joanna / Lasala, Fátima / Pérez-Rivilla, Alfredo / Delgado, Rafael

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

2021 Volume 27, Issue 6, Page(s) 886–891

Abstract: Objectives: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of ... ...

Abstract	Objectives: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of neutralizing activity in paired sera. Methods: We performed cell cultures on 193 real-time reverse transcription polymerase chain reaction respiratory samples, positive for SARS-CoV-2, obtained from 189 patients at various times, from clinical diagnosis to follow-up. Eleven samples were obtained from asymptomatic individuals, 91 samples from 91 outpatients with mild forms of COVID-19 and 91 samples from 87 inpatients with severe pneumonia. In these patients, neutralizing activity was analysed in 30 paired sera collected after symptom onset >10 days. Results: We detected a cytopathic effect (CPE) in 91/193 (47%) samples. Viral viability was maintained for up to 10 days in patients with mild COVID-19. In patients with severe COVID-19, the virus remained viable for up to 32 days after the onset of symptoms. Patients with severe COVID-19 presented infectious virus at a significantly higher rate in the samples with moderate to low viral load (cycle threshold value ≥ 26): 32/75 (43%) versus 14/63 (22%) for mild cases (p < 0.01). We observed a positive CPE despite the presence of clear neutralizing activity (NT50 > 1:1024 in 10% (3/30) of samples. Discussion: Patients with severe COVID-19 might shed viable virus during prolonged periods of up to 4 weeks after symptom onset, even when presenting high cycle threshold values in their respiratory samples and despite having developed high neutralizing antibody titres.
MeSH term(s)	Adult ; Aged ; Animals ; Antibodies, Viral/blood ; COVID-19/immunology ; COVID-19/virology ; COVID-19 Nucleic Acid Testing ; Cell Culture Techniques ; Chlorocebus aethiops ; Cytopathogenic Effect, Viral ; Female ; Hospitalization ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Male ; Microbial Viability ; Middle Aged ; Pneumonia, Viral ; SARS-CoV-2/growth & development ; SARS-CoV-2/pathogenicity ; Serologic Tests ; Severity of Illness Index ; Time Factors ; Vero Cells ; Viral Load ; Virus Cultivation ; Virus Shedding ; Young Adult
Chemical Substances	Antibodies, Viral ; Immunoglobulin G ; Immunoglobulin M
Language	English
Publishing date	2021-02-22
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	1328418-6
ISSN	1469-0691 ; 1470-9465 ; 1198-743X
ISSN (online)	1469-0691
ISSN	1470-9465 ; 1198-743X
DOI	10.1016/j.cmi.2021.02.014
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4541: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 284: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Dried Blood Spot Testing for Detection of Congenital Cytomegalovirus.

Blázquez-Gamero, Daniel / Sánchez, Blanca / Folgueira, María Dolores

JAMA pediatrics

2021 Volume 175, Issue 8, Page(s) 865–866

MeSH term(s)	Cytomegalovirus ; Cytomegalovirus Infections/diagnosis ; Dried Blood Spot Testing ; Humans
Language	English
Publishing date	2021-05-19
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	2701223-2
ISSN	2168-6211 ; 2168-6203
ISSN (online)	2168-6211
ISSN	2168-6203
DOI	10.1001/jamapediatrics.2021.0755
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Um IV Zs.74: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Derivation and external validation of a simple prediction rule for the development of respiratory failure in hospitalized patients with influenza.

Ayuso, Blanca / Lalueza, Antonio / Arrieta, Estibaliz / Romay, Eva María / Marchán-López, Álvaro / García-País, María José / Folgueira, Dolores / Gude, María José / Cueto, Cecilia / Serrano, Antonio / Lumbreras, Carlos

Respiratory research

2022 Volume 23, Issue 1, Page(s) 323

Abstract: Background: Influenza viruses cause seasonal epidemics worldwide with a significant morbimortality burden. Clinical spectrum of Influenza is wide, being respiratory failure (RF) one of its most severe complications. This study aims to elaborate a ... ...

Abstract	Background: Influenza viruses cause seasonal epidemics worldwide with a significant morbimortality burden. Clinical spectrum of Influenza is wide, being respiratory failure (RF) one of its most severe complications. This study aims to elaborate a clinical prediction rule of RF in hospitalized Influenza patients. Methods: A prospective cohort study was conducted during two consecutive Influenza seasons (December 2016-March 2017 and December 2017-April 2018) including hospitalized adults with confirmed A or B Influenza infection. A prediction rule was derived using logistic regression and recursive partitioning, followed by internal cross-validation. External validation was performed on a retrospective cohort in a different hospital between December 2018 and May 2019. Results: Overall, 707 patients were included in the derivation cohort and 285 in the validation cohort. RF rate was 6.8% and 11.6%, respectively. Chronic obstructive pulmonary disease, immunosuppression, radiological abnormalities, respiratory rate, lymphopenia, lactate dehydrogenase and C-reactive protein at admission were associated with RF. A four category-grouped seven point-score was derived including radiological abnormalities, lymphopenia, respiratory rate and lactate dehydrogenase. Final model area under the curve was 0.796 (0.714-0.877) in the derivation cohort and 0.773 (0.687-0.859) in the validation cohort (p < 0.001 in both cases). The predicted model showed an adequate fit with the observed results (Fisher's test p > 0.43). Conclusion: we present a simple, discriminating, well-calibrated rule for an early prediction of the development of RF in hospitalized Influenza patients, with proper performance in an external validation cohort. This tool can be helpful in patient's stratification during seasonal Influenza epidemics.
MeSH term(s)	Adult ; Humans ; Influenza, Human/diagnosis ; Influenza, Human/epidemiology ; Retrospective Studies ; Prospective Studies ; Respiratory Insufficiency/diagnosis ; Respiratory Insufficiency/epidemiology ; Respiratory Insufficiency/complications ; Lymphopenia/complications ; Lactate Dehydrogenases
Chemical Substances	Lactate Dehydrogenases (EC 1.1.-)
Language	English
Publishing date	2022-11-24
Publishing country	England
Document type	Journal Article
ZDB-ID	2041675-1
ISSN	1465-993X ; 1465-993X
ISSN (online)	1465-993X
ISSN	1465-993X
DOI	10.1186/s12931-022-02245-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The emergence, impact, and evolution of human metapneumovirus variants from 2014 to 2021 in Spain.

Piñana, Maria / González-Sánchez, Alejandra / Andrés, Cristina / Abanto, Michel / Vila, Jorgina / Esperalba, Juliana / Moral, Noelia / Espartosa, Elena / Saubi, Narcís / Creus, Anna / Codina, Maria Gema / Folgueira, Dolores / Martinez-Urtaza, Jaime / Pumarola, Tomàs / Antón, Andrés

The Journal of infection

2023 Volume 87, Issue 2, Page(s) 103–110

Abstract: Background: Human metapneumovirus (HMPV) is an important aetiologic agent of respiratory tract infection (RTI). This study aimed to describe the prevalence, genetic diversity, and evolutionary dynamics of HMPV.: Methods: Laboratory-confirmed HMPV ... ...

Abstract	Background: Human metapneumovirus (HMPV) is an important aetiologic agent of respiratory tract infection (RTI). This study aimed to describe the prevalence, genetic diversity, and evolutionary dynamics of HMPV. Methods: Laboratory-confirmed HMPV were characterised based on partial-coding G gene sequences with MEGA.v6.0. WGS was performed with Illumina, and evolutionary analyses with Datamonkey and Nextstrain. Results: HMPV prevalence was 2.5%, peaking in February-April and with an alternation in the predominance of HMPV-A and -B until the emergence of SARS-CoV-2, not circulating until summer and autumn-winter 2021, with a higher prevalence and with the almost only circulation of A2c Conclusion: HMPV showed a significant morbidity until the emergence of SARS-CoV-2 pandemic in 2020, not circulating again until summer and autumn 2021, with a higher prevalence and with almost the only circulation of A2c
MeSH term(s)	Humans ; Infant ; Metapneumovirus/genetics ; Paramyxoviridae Infections/epidemiology ; Spain/epidemiology ; Genotype ; COVID-19/epidemiology ; SARS-CoV-2/genetics ; Respiratory Tract Infections/epidemiology ; Phylogeny
Language	English
Publishing date	2023-05-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	424417-5
ISSN	1532-2742 ; 0163-4453
ISSN (online)	1532-2742
ISSN	0163-4453
DOI	10.1016/j.jinf.2023.05.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1501: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 48: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Genetic polymorphisms in TLR3, IL10 and CD209 influence the risk of BK polyomavirus infection after kidney transplantation.

Redondo, Natalia / Rodríguez-Goncer, Isabel / Parra, Patricia / López-Medrano, Francisco / González, Esther / Hernández, Ana / Trujillo, Hernando / Ruiz-Merlo, Tamara / San Juan, Rafael / Folgueira, María Dolores / Andrés, Amado / Aguado, José María / Fernández-Ruiz, Mario

Scientific reports

2022 Volume 12, Issue 1, Page(s) 11338

Abstract: Genetic determinants of BK polyomavirus infection after kidney transplantation remain poorly investigated. We assessed the potential impact of 13 different single nucleotide polymorphisms within genes mainly involved in innate immune responses on the ... ...

Abstract	Genetic determinants of BK polyomavirus infection after kidney transplantation remain poorly investigated. We assessed the potential impact of 13 different single nucleotide polymorphisms within genes mainly involved in innate immune responses on the risk of BKPyV viremia in 204 KT recipients. After a median follow-up of 1121.5 days, the cumulative incidence of any-level BKPyV viremia was 24.5% (50/204). There was a significant association between the minor T allele of TLR3 (rs3775291) SNP and the development of BKPyV viremia (adjusted hazard ratio [aHR]: 2.16; 95% confidence interval [CI]: 1.08-4.30; P value = 0.029), whereas the minor G allele of CD209 (rs4804803) SNP exerted a protective role (aHR: 0.54; 95% CI: 0.29-1.00; P value = 0.050). A higher incidence of BKPyV viremia was also observed for the minor G allele of IL10 (rs1800872) SNP, although the absence of BKPyV events among homozygotes for the reference allele prevented multivariable analysis. The BKPyV viremia-free survival rate decreased with the increasing number of unfavorable genotypes (100% [no unfavorable genotypes], 85.4% [1 genotype], 70.9% [2 genotypes], 52.5% [3 genotypes]; P value = 0.008). In conclusion, SNPs in TLR3, CD209 and IL10 genes play a role in modulating the susceptibility to any-level BKPyV viremia among KT recipients.
MeSH term(s)	BK Virus/physiology ; Cell Adhesion Molecules/genetics ; Genetic Predisposition to Disease ; Humans ; Interleukin-10/genetics ; Kidney Transplantation ; Lectins, C-Type/genetics ; Polymorphism, Single Nucleotide ; Polyomavirus Infections ; Receptors, Cell Surface/genetics ; Toll-Like Receptor 3/genetics ; Viremia/epidemiology
Chemical Substances	Cell Adhesion Molecules ; DC-specific ICAM-3 grabbing nonintegrin ; IL10 protein, human ; Lectins, C-Type ; Receptors, Cell Surface ; TLR3 protein, human ; Toll-Like Receptor 3 ; Interleukin-10 (130068-27-8)
Language	English
Publishing date	2022-07-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-15406-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Cytomegalovirus Exposure and the Risk of Overall Infection After Kidney Transplantation: A Cohort Study on the Indirect Effects Attributable to Viral Replication.

Rodríguez-Goncer, Isabel / Ruiz-Ruigómez, María / López-Medrano, Francisco / Trujillo, Hernando / González, Esther / Polanco, Natalia / Gutiérrez, Eduardo / San Juan, Rafael / Corbella, Laura / Ruiz-Merlo, Tamara / Parra, Patricia / Folgueira, María Dolores / Andrés, Amado / Aguado, José María / Fernández-Ruiz, Mario

Transplant international : official journal of the European Society for Organ Transplantation

2022 Volume 35, Page(s) 10273

Abstract: Previous reports hypothesized that cytomegalovirus (CMV) may predispose to non-CMV infection after kidney transplantation (KT). We analysed the incidence of non-CMV infection (overall, bacterial and opportunistic) in 291 KT recipients according to the ... ...

Abstract	Previous reports hypothesized that cytomegalovirus (CMV) may predispose to non-CMV infection after kidney transplantation (KT). We analysed the incidence of non-CMV infection (overall, bacterial and opportunistic) in 291 KT recipients according to the previous development of any level or high-level (≥1,000 IU/ml) CMV viremia. Exposure to CMV replication was assessed throughout fixed intervals covering first the 30, 90, 180 and 360 post-transplant days (cumulative exposure) and non-overlapping preceding periods (recent exposure). Adjusted Cox models were constructed for each landmark analysis. Overall, 67.7 and 50.5% patients experienced non-CMV and CMV infection, respectively. Patients with cumulative CMV exposure had higher incidence of non-CMV infection beyond days 30 (
MeSH term(s)	Antiviral Agents ; Cohort Studies ; Cytomegalovirus ; Cytomegalovirus Infections/epidemiology ; Cytomegalovirus Infections/etiology ; Humans ; Kidney Transplantation/adverse effects ; Viral Load ; Virus Replication
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2022-01-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	639435-8
ISSN	1432-2277 ; 0934-0874
ISSN (online)	1432-2277
ISSN	0934-0874
DOI	10.3389/ti.2021.10273
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2358: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Clinical outcomes and antibody transfer in a cohort of infants with in utero or perinatal exposure to SARS-CoV-2 (Coronascope Study).

European journal of pediatrics

2023 Volume 182, Issue 10, Page(s) 4647–4654

Abstract: We aimed to describe the outcomes, focusing on the hearing and neurological development, of infants born to mothers with COVID-19 during pregnancy and to evaluate the persistence of maternal antibodies in the first months of life. An observational, ... ...

Abstract	We aimed to describe the outcomes, focusing on the hearing and neurological development, of infants born to mothers with COVID-19 during pregnancy and to evaluate the persistence of maternal antibodies in the first months of life. An observational, prospective study at a tertiary hospital in Madrid (Spain) on infants born to mothers with COVID-19 during pregnancy between March and September 2020 was conducted. A follow-up visit at 1-3 months of age with a physical and neurological examination, cranial ultrasound (cUS), SARS-CoV-2 RT-PCR on nasopharyngeal swab, and SARS-CoV-2 serology were performed. Hearing was evaluated at birth through the automated auditory brainstem response and at six months of age through the auditory steady-state response. A neurodevelopmental examination using the Bayley-III scale was performed at 12 months of age. Of 95 infants studied, neurological examination was normal in all of them at the follow-up visit, as was the cUS in 81/85 (95%) infants, with only mild abnormalities in four of them. Serology was positive in 47/95 (50%) infants, which was not associated with symptoms or severity of maternal infection. No hearing loss was detected, and neurodevelopment was normal in 96% of the infants (median Z score: 0). Conclusion: In this cohort, the majority of infants born to mothers with COVID-19 during pregnancy were healthy infants with a normal cUS, no hearing loss, and normal neurodevelopment in the first year of life. Only half of the infants had a positive serological result during the follow-up. What is known: • Hearing loss and neurodevelopmental delay in infants born to mothers with COVID-19 during pregnancy has been suggested, although data is inconsistent. Maternal antibody transfer seems to be high, with a rapid decrease during the first weeks of life. What is new: • Most infants born to mothers with COVID-19 during pregnancy had normal hearing screening, cranial ultrasound, and neurodevelopmental status at 12 months of life. Antibodies against SARS-CoV-2 were only detected in 50% of the infants at two months of life.
MeSH term(s)	Infant, Newborn ; Pregnancy ; Female ; Humans ; Infant ; SARS-CoV-2 ; COVID-19/diagnosis ; Prospective Studies ; Spain/epidemiology ; Pregnancy Complications, Infectious/diagnosis ; Pregnancy Complications, Infectious/prevention & control ; Infectious Disease Transmission, Vertical/prevention & control
Language	English
Publishing date	2023-08-10
Publishing country	Germany
Document type	Journal Article
ZDB-ID	194196-3
ISSN	1432-1076 ; 0340-6199 ; 0943-9676
ISSN (online)	1432-1076
ISSN	0340-6199 ; 0943-9676
DOI	10.1007/s00431-023-05147-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Um IV Zs.75: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: The role of the T-cell mediated immune response to Cytomegalovirus infection in intrauterine transmission.

Soriano-Ramos, María / Esquivel-De la Fuente, Estrella / Albert Vicent, Eliseo / de la Calle, María / Baquero-Artigao, Fernando / Domínguez-Rodríguez, Sara / Cabanes, María / Gómez-Montes, Enery / Goncé, Anna / Valdés-Bango, Marta / Viñuela-Benéitez, Mª Carmen / Muñoz-Chápuli Gutiérrez, Mar / Saavedra-Lozano, Jesús / Cuadrado Pérez, Irene / Encinas, Begoña / Castells Vilella, Laura / de la Serna Martínez, María / Tagarro, Alfredo / Rodríguez-Molino, Paula /

Giménez Quiles, Estela / García Alcázar, Diana / García Burguillo, Antonio / Folgueira, María Dolores / Navarro, David / Blázquez-Gamero, Daniel

PloS one

2023 Volume 18, Issue 2, Page(s) e0281341

Abstract: Introduction: Prognostic markers for fetal transmission of Cytomegalovirus (CMV) infection during pregnancy are poorly understood. Maternal CMV-specific T-cell responses may help prevent fetal transmission and thus, we set out to assess whether this may ...

Abstract	Introduction: Prognostic markers for fetal transmission of Cytomegalovirus (CMV) infection during pregnancy are poorly understood. Maternal CMV-specific T-cell responses may help prevent fetal transmission and thus, we set out to assess whether this may be the case in pregnant women who develop a primary CMV infection. Methods: A multicenter prospective study was carried out at 8 hospitals in Spain, from January 2017 to April 2020. Blood samples were collected from pregnant women at the time the primary CMV infection was diagnosed to assess the T-cell response. Quantitative analysis of interferon producing specific CMV-CD8+/CD4+ cells was performed by intracellular cytokine flow cytometry. Results: In this study, 135 pregnant women with a suspected CMV infection were evaluated, 60 of whom had a primary CMV infection and samples available. Of these, 24 mothers transmitted the infection to the fetus and 36 did not. No association was found between the presence of specific CD4 or CD8 responses against CMV at the time maternal infection was diagnosed and the risk of fetal transmission. There was no transmission among women with an undetectable CMV viral load in blood at diagnosis. Conclusions: In this cohort of pregnant women with a primary CMV infection, no association was found between the presence of a CMV T-cell response at the time of maternal infection and the risk of intrauterine transmission. A detectable CMV viral load in the maternal blood at diagnosis of the primary maternal infection may represent a relevant biomarker associated with fetal transmission.
MeSH term(s)	Pregnancy ; Female ; Humans ; Cytomegalovirus ; Pregnancy Complications, Infectious ; Prospective Studies ; Cytomegalovirus Infections ; CD8-Positive T-Lymphocytes ; Infectious Disease Transmission, Vertical/prevention & control ; Immunity
Language	English
Publishing date	2023-02-06
Publishing country	United States
Document type	Multicenter Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0281341
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito