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Article: Effect of electroacupuncture of "Jiaji" (EX-B2) on NOD-like receptor protein 3 mediated pyroptosis in spinal cord tissue of rats with acute spinal cord injury.

Mei, Ji-Lin / Yang, Zhi-Xin / Li, Xiao-Ning

2024 Volume 49, Issue 2, Page(s) 110–118

Abstract: Objectives: To observe the effect of electroacupuncture (EA) stimulation of "Jiaji"(EX-B2) on motor function, histomorphology, and expression of NOD-like receptor protein 3 (NLRP3) and N-terminal domain of gasdermin D (GSDMD-N) in the spinal cord tissue ...

Title translation	夹脊电针对急性脊髓损伤大鼠脊髓组织NOD样受体热蛋白结构域相关蛋白3介导的细胞焦亡的影响.
Abstract	Objectives: To observe the effect of electroacupuncture (EA) stimulation of "Jiaji"(EX-B2) on motor function, histomorphology, and expression of NOD-like receptor protein 3 (NLRP3) and N-terminal domain of gasdermin D (GSDMD-N) in the spinal cord tissue of rats with spinal cord injury (SCI), so as to explore its mechanism underlying improvement of SCI. Methods: Forty eight female SD rats were randomly divided into sham surgery (sham), SCI model (model), EA, and NLRP3 agonist (monosodium urate, MSU) combined with Jiaji EA (MSU+EA) groups, with 12 rats in each group which were further divided into 3 d and 7 d subgroups, with 6 rats at each time point. Two EA groups received EA stimulation of EX-B2 with a frequency of 100 Hz, electrical current of 1-2 mA for 30 min, once a day for 3 or 7 days. After 5 min, 6 h, and 24 h of modeling, rats of the MSU+EA group received intraperitoneal injection of MSU (200 μg/kg, 200 μg/mL) . The motor function was evaluated using Basso-Beattie-Bresnahan (BBB) scale, the morphological structure of rat spinal cord tissue was observed by H.E. staining. The expression of pyroptosis related factors NLRP3, cleaved Caspase-1 and GSDMD-N of the spinal cord was observed by using immunohistochemistry and Western blot separately, the expression and localization of Iba-1 and GSDMD-N in the spinal cord tissue were observed using immunofluorescence double staining method. Results: Compared with the sham group, the BBB scores after modeling and on day 3 and 7 were decreased ( Conclusions: EA of EX-B2 can improve the motor function of SCI rats, which may be related to its functions in inhibiting pyroptosis of microglia mediated by NLRP3/Caspase-1 signaling pathway.
MeSH term(s)	Animals ; Female ; Rats ; Caspase 1 ; Caspases ; Electroacupuncture ; NLR Family, Pyrin Domain-Containing 3 Protein/genetics ; Pyroptosis ; Rats, Sprague-Dawley ; Spinal Cord ; Spinal Cord Injuries/genetics ; Spinal Cord Injuries/therapy
Chemical Substances	Caspase 1 (EC 3.4.22.36) ; Caspases (EC 3.4.22.-) ; NLR Family, Pyrin Domain-Containing 3 Protein ; Nlrp3 protein, rat
Language	Chinese
Publishing date	2024-02-27
Publishing country	China
Document type	Journal Article
ZDB-ID	1283179-7
ISSN	1000-0607
ISSN	1000-0607
DOI	10.13702/j.1000-0607.20230701
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Article: [Research progress of acupuncture-moxibustion in treatment of spinal cord injury through mediating inflammatory reaction].

Mei, Ji-Lin / Yang, Zhi-Xin / Li, Xiao-Ning

Zhen ci yan jiu = Acupuncture research

2023 Volume 48, Issue 7, Page(s) 699–705

Abstract: Inflammatory reaction after spinal cord injury (SCI) is the main obstacle to the recovery of neural function. In the occurrence and development of SCI, the complex regulatory mechanisms are involved in inflammatory reaction, including the activation of ... ...

Abstract	Inflammatory reaction after spinal cord injury (SCI) is the main obstacle to the recovery of neural function. In the occurrence and development of SCI, the complex regulatory mechanisms are involved in inflammatory reaction, including the activation of inflammatory cells (e.g.macrophages and microglia), and the release of cytokines (e.g. tumor necrosis factor, interleukin and chemokines). Acupuncture-moxibustion is significantly effective in clinical treatment of SCI, and its mechanism is related to adjusting the function of inflammatory cells after SCI, modulating the expression of cytokines and the activation of NLRP3 inflammatory bodies, as well as the expression of high mobility group protein B1 and calcitonin gene related peptide. This article summarizes the research progress of mechanism of inflammatory reaction after SCI and the effects of acupuncture-moxibustion intervention in recent years so that the new ideas can be provided to clarify the pathogenesis of SCI and the effect of acupuncture-moxibustion intervention.
MeSH term(s)	Humans ; Moxibustion ; Spinal Cord Injuries/therapy ; Acupuncture Therapy ; Cytokines/metabolism ; Macrophages/metabolism ; Macrophages/pathology ; Inflammation/therapy ; Spinal Cord
Chemical Substances	Cytokines
Language	Chinese
Publishing date	2023-07-31
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1283179-7
ISSN	1000-0607
ISSN	1000-0607
DOI	10.13702/j.1000-0607.20221181
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Article: [The Regulatory Effect of RNA m

Li, Yu-Qing / Shao, Yang-Liu / Li, Meng-Yue / Wang, Li-Li / Gao, Xiao-Ning

Zhongguo shi yan xue ye xue za zhi

2024 Volume 32, Issue 2, Page(s) 382–388

Abstract: Objective: To confirm the direct regulatory effect of : Methods: The lentivirus-mediated shRNA target : Results: Silencing the expression of : Conclusion: In t(8;21) AML cell lines, ...

Abstract	Objective: To confirm the direct regulatory effect of Methods: The lentivirus-mediated shRNA target Results: Silencing the expression of Conclusion: In t(8;21) AML cell lines,
MeSH term(s)	Humans ; Jumonji Domain-Containing Histone Demethylases/genetics ; Leukemia, Myeloid, Acute/genetics ; Cell Line, Tumor ; Methylation ; RNA, Messenger/genetics ; RNA, Small Interfering/genetics
Chemical Substances	Jumonji Domain-Containing Histone Demethylases (EC 1.14.11.-) ; KDM4B protein, human (EC 1.14.11.-) ; RNA, Messenger ; RNA, Small Interfering
Language	Chinese
Publishing date	2024-04-25
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	2404306-0
ISSN	1009-2137
ISSN	1009-2137
DOI	10.19746/j.cnki.issn.1009-2137.2024.02.009
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Article ; Online: Analysis of the lncRNA-Associated Competing Endogenous RNA (ceRNA) Network for Tendinopathy.

Huang, Bing-Zhe / Jing-Jing, Yang / Dong, Xiao-Ming / Zhuan Zhong / Xiao-Ning Liu

Genetics research

2022 Volume 2022, Page(s) 9792913

Abstract: Background: We aimed to construct the lncRNA-associated competing endogenous RNA (ceRNA) network and distinguish feature lncRNAs associated with tendinopathy.: Methods: We downloaded the gene profile of GSE26051 from the Gene Expression Omnibus (GEO), ...

Abstract	Background: We aimed to construct the lncRNA-associated competing endogenous RNA (ceRNA) network and distinguish feature lncRNAs associated with tendinopathy. Methods: We downloaded the gene profile of GSE26051 from the Gene Expression Omnibus (GEO), including 23 normal samples and 23 diseased tendons. Differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs) were identified, and functional and pathway enrichment analyses were performed. Protein-protein interaction (PPI) network was constructed and further analyzed by module mining. Moreover, a ceRNA regulatory network was constructed based on the identified lncRNA-mRNA coexpression relationship pairs and miRNA-mRNA regulation pairs. Results: We identified 1117 DEmRNAs and 57 DElncRNAs from the GEO data. The downregulated DEmRNAs were particularly associated with muscle contraction and muscle filament, while the upregulated ones were linked to extracellular matrix organization and cell adhesion. From the PPI network, 11 modules were extracted. Genes in MCODE 2 (such as TPM4) were significantly involved in cardiomyopathy, and genes in MCODE 4 (such as COL4A3 and COL4A4) were involved in focal adhesion, ECM-receptor interaction, and PI3K-Akt signaling pathway. The ceRNA network contained 7 lncRNAs (MIR133A1HG, LINC01405, PRKCQ-AS1, C10orf71-AS1, MBNL1-AS1, HOTAIRM1, and DNM3OS), 63 mRNAs, and 41 miRNAs. Downregulated lncRNA MIR133A1HG could competitively bind with hsa-miR-659-3p and hsa-miR-218-1-3p to regulate the TPM3. Meanwhile, MIR133A1HG could competitively bind with hsa-miR-1179 to regulate the COL4A3. Downregulated C10orf71-AS1 could competitively bind with hsa-miR-130a-5p to regulate the COL4A4. Conclusions: Seven important lncRNAs, particularly MIR133A1HG and C10orf71-AS1, were found associated with tendinopathy according to the lncRNA-associated ceRNA network.
MeSH term(s)	Gene Regulatory Networks/genetics ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Phosphatidylinositol 3-Kinases/genetics ; RNA, Long Noncoding/genetics ; RNA, Long Noncoding/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Tendinopathy/genetics
Chemical Substances	MIRN1179 microRNA, human ; MIRN218 microRNA, human ; MicroRNAs ; RNA, Long Noncoding ; RNA, Messenger
Language	English
Publishing date	2022-05-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2412681-0
ISSN	1469-5073 ; 0016-6723
ISSN (online)	1469-5073
ISSN	0016-6723
DOI	10.1155/2022/9792913
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Article ; Online: Proteinase‑3‑antineutrophil cytoplasmic antibody‑associated vasculitis secondary to subacute infective endocarditis: A case report.

Lu, Hui / Cui, Zhao / Zhou, Xu-Jie / Yang, Ying / Han, Xiao-Ning / Li, Xi-Hui / Zhou, Fu-De / Zhao, Ming-Hui

Experimental and therapeutic medicine

2024 Volume 27, Issue 5, Page(s) 216

Abstract: A 58-year-old male patient was admitted to Peking University First Hospital (Beijing, China) due to recurrent hematuria, proteinuria and kidney dysfunction. The patient was positive for proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA). ... ...

Abstract	A 58-year-old male patient was admitted to Peking University First Hospital (Beijing, China) due to recurrent hematuria, proteinuria and kidney dysfunction. The patient was positive for proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA). Pathology of the kidney showed focal proliferative necrotizing glomerulonephritis with crescent formation and immune complex-mediated glomerulonephritis. The patient was diagnosed with PR3-ANCA-associated vasculitis (AAV), received intensive immunosuppressive therapy and experienced two relapses within 1 year. After admission, aortic valve vegetation was observed via echocardiography. The patient subsequently received antibiotic treatment and valve replacement, and achieved complete remission of kidney and cardiac function. The present case emphasized the importance of identifying secondary reasons for ANCA formation, especially infective endocarditis in patients with PR3-AAV.
Language	English
Publishing date	2024-03-20
Publishing country	Greece
Document type	Case Reports
ZDB-ID	2683844-8
ISSN	1792-1015 ; 1792-0981
ISSN (online)	1792-1015
ISSN	1792-0981
DOI	10.3892/etm.2024.12504
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Article: [Distribution Prediction of Soil Heavy Metals Based on Remote Sensing Temporal-Spatial-Spectral Features and Random Forest Model].

Wang, Ze-Qiang / Zhang, Dong-You / Xu, Xi-Bo / Wang, Zhao-Peng / Yang, Dong-Yu / Song, Xiao-Ning

Huan jing ke xue= Huanjing kexue

2024 Volume 45, Issue 3, Page(s) 1713–1723

Abstract: Obtaining soil heavy metal content characteristics and spatial distribution is crucial for preventing soil pollution and formulating environmental protection policies. We collected 304 surface soil samples (0-20 cm) in the Changqing district. At the same ...

Abstract	Obtaining soil heavy metal content characteristics and spatial distribution is crucial for preventing soil pollution and formulating environmental protection policies. We collected 304 surface soil samples (0-20 cm) in the Changqing district. At the same time, the spectral, temporal, and spatial features of soil heavy metals were derived from multi-remote sensing data; the temporal-spatial-spectral features closely related to soil heavy metals were selected via correlation analysis and used as input independent variables. The measured soil arsenic (As) content was used as the dependent variable to establish a spatial prediction model based on the random forest (RF) algorithm. The results showed the following:the As content in the soils exceeded the background value by 43.17% but did not exceed the risk screening values and intervention values, indicating slight heavy metal pollution in the soil. The accuracy ranking of the spatial prediction models with one feature type from high to low was spatial features (ratio of performance to inter-quartile range (RPIQ)=3.87)>temporal features (RPIQ=2.57)>spectral features (RPIQ=2.50). The spatial features were the most informative for predicting soil heavy metals. The models using temporal-spatial, temporal-spectral, and spatial-spectral features were superior to those using only one feature type, and the RPIQ values were 4.81, 4.21, and 4.70, respectively. The RF model with temporal-spatial-spectral features achieved the highest spatial prediction accuracy (
Language	Chinese
Publishing date	2024-03-12
Publishing country	China
Document type	English Abstract ; Journal Article
ISSN	0250-3301
ISSN	0250-3301
DOI	10.13227/j.hjkx.202301103
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Article: Esmolol increases the fecal abundance of Lactobacillus in a rat model of sepsis.

Yao, Bo / Wang, Fu-Hua / Han, Xiao-Ning / Yang, Jun / Xue, Ping / Qi, Qi / Wei, Guang-Yao / Xing, Jin-Yan

Intensive care medicine experimental

2024 Volume 12, Issue 1, Page(s) 22

Abstract: Background: Disorders of the gut microbiome could be responsible for the progression of multiple organ dysfunction syndrome. In this study, we examined the effect of esmolol on the gut microbiome in a rat model of sepsis induced by cecal ligation and ... ...

Abstract	Background: Disorders of the gut microbiome could be responsible for the progression of multiple organ dysfunction syndrome. In this study, we examined the effect of esmolol on the gut microbiome in a rat model of sepsis induced by cecal ligation and puncture (CLP). Methods: The animals (n = 32) were randomly divided into 3 groups: Sham group (sham operation + normal saline treatment, n = 8), CLP group (cecal ligation and puncture + normal saline treatment, n = 12), and CLP + ESM group (cecal ligation and puncture + esmolol treatment, n = 12). After 24 h, feces in the colon were collected for 16S rRNA gene sequencing and nitric oxide analysis. In addition, colon was removed for immunohistochemical staining of inducible nitric oxide synthase (iNOS). Results: Four rats in the CLP group and two rats in the CLP + ESM group died. The abundance of Lactobacillus in the CLP + ESM group was higher than CLP group (P = 0.048). In the linear discriminant analysis effect size analysis, Norank f Muribaculaceae, Escherichia-Shigella and Lactobacillus were the predominant bacteria in the Sham group, CLP group and CLP + ESM group, respectively. The iNOS expression in colonocytes stained by brown in the CLP group were much more than Sham group (P = 0.001). Compared to CLP group, the iNOS expression in colonocytes reduced after esmolol treatment (P = 0.013). The concentration of nitric oxide in colon feces was different in Sham group, CLP group and CLP + ESM group (1.31 ± 0.15μmmol/l vs. 1.98 ± 0.27μmmol/l vs. 1.51 ± 0.14μmmol/l, P = 0.001). In addition, the concentration of nitric oxide in CLP group was higher than Sham group (P = 0.001) or CLP + ESM group (P = 0.001). Conclusions: Esmolol increased the fecal abundance of Lactobacillus in a rat model of sepsis. Moreover, esmolol reduced the iNOS expression of colonocytes and the nitric oxide concentration of colon feces.
Language	English
Publishing date	2024-03-04
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2740385-3
ISSN	2197-425X
ISSN	2197-425X
DOI	10.1186/s40635-023-00589-1
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Article ; Online: Association of beta-blocker therapy at discharge with clinical outcomes in patients without heart failure or left ventricular systolic dysfunction after acute coronary syndrome: An updated systematic review and meta-analysis.

Hu, Meng-Jin / Wang, Xiao-Ning / Tan, Jiang-Shan / Yang, Yue-Jin

Archives of cardiovascular diseases

2022

Abstract: Background: Beta-blockers are the standard treatment for acute coronary syndrome (ACS) based on evidence from the prethrombolytic era. We sought to examine the effect of beta-blocker treatment on patients without heart failure or left ventricular ... ...

Abstract	Background: Beta-blockers are the standard treatment for acute coronary syndrome (ACS) based on evidence from the prethrombolytic era. We sought to examine the effect of beta-blocker treatment on patients without heart failure or left ventricular systolic dysfunction after ACS in the contemporary percutaneous coronary intervention (PCI) era. Methods: We systematically searched PubMed, Web of Science, Cochrane Library, ClinicalTrials.gov and Google Scholar for studies comparing beta-blockers versus no beta-blockers in ACS patients in the contemporary PCI era. The primary outcome was all-cause death. Pooling unadjusted and multivariable adjusted results were calculated under random-effects models. Results: Data from 15 studies (n=205,672), including 1 randomized trial, were analysed. Compared with no beta-blockers, beta-blocker therapy at discharge may reduce the risk of all-cause death (odds ratio [OR] 0.66, 95% confidence interval [CI]: 0.50-0.86; I Conclusions: In patients without heart failure or left ventricular systolic dysfunction after ACS in the contemporary PCI era, beta-blocker therapy may still be beneficial due to a potential reduced risk of all-cause death.
Language	English
Publishing date	2022-10-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2408778-6
ISSN	1875-2128 ; 1875-2136
ISSN (online)	1875-2128
ISSN	1875-2136
DOI	10.1016/j.acvd.2022.09.004
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Article ; Online: Yan Xiaoning’s experience in treating erythema nodule

Xue-Yuan Yang / Xiao-Ning Yan

Journal of Hainan Medical University, Vol 26, Iss 18, Pp 61-

2020 Volume 64

Abstract: ... skin under the successive generations of the successor, unique, unique experience. Yan xiaoning ...

Abstract	Erythema tuberculum is a common disease in dermatology and difficult to treat. Chang an cheng's skin under the successive generations of the successor, unique, unique experience. Yan xiaoning summarized the etiology and pathogenesis of nodular erythema as heat and humidity toxicity, and divided the syndrome differentiation into two syndromes: wind and heat toxicity containing moisture and heat. The prescription drug emphasizes in the overall concept of the foundation, both inside and outside treatment, treatment of the need to reconcile, both the spleen and stomach for the specimens. In this paper, Professor Yan Xiaoning's experience in the treatment of erythema nodule is summarized, and case 1 is attached as evidence
Keywords	yan xiaoning ; erythema nodosum ; etiology and pathogenesis ; characteristics ; experience ; Medicine ; R
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	Editorial Board of Journal of Hainan Medical University
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Potential Common Mechanisms of Cytotoxicity Induced by Organophosphorus Pesticides via NLRP3 Inflammasome Activation.

Wang, Xiaoning / Sui, Xin / Sun, Yangyang / Cui, Ziqi / Ma, Ning / Wang, Shuai / Yang, Jun / Liu, Fengying / Yang, Weijie / Xiao, Zhenyu / Zhu, Tong / Luo, Yuan / Wang, Yongan

GeoHealth

2024 Volume 8, Issue 4, Page(s) e2023GH000888

Abstract: The Multi-Threat Medical Countermeasure (MTMC) technique is crucial for developing common biochemical signaling pathways, molecular mediators, and cellular processes. This study revealed that the Nod-like receptor 3 (NLRP3) inflammasome pathway may be a ... ...

Abstract	The Multi-Threat Medical Countermeasure (MTMC) technique is crucial for developing common biochemical signaling pathways, molecular mediators, and cellular processes. This study revealed that the Nod-like receptor 3 (NLRP3) inflammasome pathway may be a significant contributor to the cytotoxicity induced by various organophosphorus pesticides (OPPs). The study demonstrated that exposure to six different types of OPPs (paraoxon, dichlorvos, fenthion, dipterex, dibrom, and dimethoate) led to significant cytotoxicity in BV2 cells, which was accompanied by increased expression of NLRP3 inflammasome complexes (NLRP3, ASC, Caspase-1) and downstream inflammatory cytokines (IL-1β, IL-18), in which the order of cytotoxicity was dichlorvos > dipterex > dibrom > paraoxon > fenthion > dimethoate, based on the IC
Language	English
Publishing date	2024-04-18
Publishing country	United States
Document type	Journal Article
ISSN	2471-1403
ISSN (online)	2471-1403
DOI	10.1029/2023GH000888
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