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Article: Antifungal Mechanisms of a Chinese Herbal Medicine, Cao Huang Gui Xiang, Against

Yue, Huizhen / Xu, Xiaolong / He, Shasha / Cui, Xuran / Guo, Yuhong / Zhao, Jingxia / Peng, Bing / Liu, Qingquan

2022 Volume 13, Page(s) 813818

Abstract: Cao Huang Gui Xiang (CHGX) formula, a Chinese herbal medicine, has been empirically used ...

Abstract	Cao Huang Gui Xiang (CHGX) formula, a Chinese herbal medicine, has been empirically used for the treatment of
Language	English
Publishing date	2022-03-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.813818
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Corrigendum: Antifungal mechanisms of a Chinese herbal medicine, Cao Huang Gui Xiang, against

Yue, Huizhen / Xu, Xiaolong / He, Shasha / Cui, Xuran / Guo, Yuhong / Zhao, Jingxia / Peng, Bing / Liu, Qingquan

Frontiers in pharmacology

2022 Volume 13, Page(s) 977030

Abstract: This corrects the article DOI: 10.3389/fphar.2022.813818.]. ...

Abstract	[This corrects the article DOI: 10.3389/fphar.2022.813818.].
Language	English
Publishing date	2022-07-22
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.977030
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Integrating network pharmacology and pharmacological validation to explore the effect of Shi Wei Ru Xiang powder on suppressing hyperuricemia.

Li, Qiang / Liu, Peng / Wu, Chen / Bai, Lijie / Zhang, Zhongyun / Bao, Zilu / Zou, Min / Ren, Zhaoxiang / Yuan, Lin / Liao, Maochuan / Lan, Zhou / Yin, Shijin / Chen, Lvyi

Journal of ethnopharmacology

2022 Volume 298, Page(s) 115679

Abstract: Ethnopharmacological relevance: Shi Wei Ru Xiang powder (SWR) is a traditional Tibetan medicinal ...

Abstract	Ethnopharmacological relevance: Shi Wei Ru Xiang powder (SWR) is a traditional Tibetan medicinal formula with the effect of dispelling dampness and dispersing cold. In clinical practice, SWR is generally used for the treatment of hyperuricemia (HUA). However, its exact pharmacological mechanism remains unclear. Aims of the study: To preliminarily elucidate the regulatory effects and possible mechanisms of SWR on hyperuricemia using network pharmacology and experimental validation. Materials and methods: A mouse model of hyperuricemia was used to evaluate the alleviating effect of SWR on hyperuricemia. The major components of SWR were acquired by UPLC-Q/TOF-MS. The potential molecular targets and associated signaling pathways were predicted through network pharmacology. The mechanism of action of SWR in ameliorating hyperuricemia was further investigated by pharmacological evaluation. Results: Mice with hyperuricemia and renal dysfunction were ameliorated by SWR. The 36 components of SWR included phenolic acids, terpenoids, alkaloids and flavonoids were identified. Network pharmacological analysis showed the involvement of the above compounds, and 115 targets were involved to treat hyperuricemia, involving multiple biological processes and different signaling pathways. Pharmacological experiments validated that SWR ameliorated hyperuricemic nephropathy in mice by modulating the mitogen-activated protein kinase (MAPK) signaling pathway, nuclear factor kappaB (NF-κB) signaling pathway and NOD-like receptor signaling pathway. Conclusion: MAPK signaling pathway, NF-κB signaling pathway and NOD-like receptor signaling pathway play important roles in the therapeutic effects of SWR on hyperuricemia.
MeSH term(s)	Animals ; Drugs, Chinese Herbal/metabolism ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Hyperuricemia/drug therapy ; Hyperuricemia/metabolism ; Medicine, Tibetan Traditional ; Mice ; NF-kappa B ; NLR Proteins ; Network Pharmacology ; Powders/therapeutic use
Chemical Substances	Drugs, Chinese Herbal ; NF-kappa B ; NLR Proteins ; Powders
Language	English
Publishing date	2022-09-01
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.115679
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Article: Integrating network pharmacology and pharmacological validation to explore the effect of Shi Wei Ru Xiang powder on suppressing hyperuricemia

Li, Qiang / Liu, Peng / Wu, Chen / Bai, Lijie / Zhang, Zhongyun / Bao, Zilu / Zou, Min / Ren, Zhaoxiang / Yuan, Lin / Liao, Maochuan / Lan, Zhou / Yin, Shijin / Chen, Lvyi

Journal of ethnopharmacology. 2022 Nov. 15, v. 298

2022

Abstract: Shi Wei Ru Xiang powder (SWR) is a traditional Tibetan medicinal formula with the effect ...

Abstract	Shi Wei Ru Xiang powder (SWR) is a traditional Tibetan medicinal formula with the effect of dispelling dampness and dispersing cold. In clinical practice, SWR is generally used for the treatment of hyperuricemia (HUA). However, its exact pharmacological mechanism remains unclear. To preliminarily elucidate the regulatory effects and possible mechanisms of SWR on hyperuricemia using network pharmacology and experimental validation. A mouse model of hyperuricemia was used to evaluate the alleviating effect of SWR on hyperuricemia. The major components of SWR were acquired by UPLC-Q/TOF-MS. The potential molecular targets and associated signaling pathways were predicted through network pharmacology. The mechanism of action of SWR in ameliorating hyperuricemia was further investigated by pharmacological evaluation. Mice with hyperuricemia and renal dysfunction were ameliorated by SWR. The 36 components of SWR included phenolic acids, terpenoids, alkaloids and flavonoids were identified. Network pharmacological analysis showed the involvement of the above compounds, and 115 targets were involved to treat hyperuricemia, involving multiple biological processes and different signaling pathways. Pharmacological experiments validated that SWR ameliorated hyperuricemic nephropathy in mice by modulating the mitogen-activated protein kinase (MAPK) signaling pathway, nuclear factor kappaB (NF-κB) signaling pathway and NOD-like receptor signaling pathway. MAPK signaling pathway, NF-κB signaling pathway and NOD-like receptor signaling pathway play important roles in the therapeutic effects of SWR on hyperuricemia.
Keywords	cold ; flavonoids ; hyperuricemia ; kidney diseases ; mechanism of action ; mice ; mitogen-activated protein kinase ; models ; pharmacology ; terpenoids ; therapeutics ; traditional medicine ; transcription factor NF-kappa B
Language	English
Dates of publication	2022-1115
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2022.115679
Database	NAL-Catalogue (AGRICOLA)

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Article: Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate.

Zheng, Changbing / Song, Bo / Guo, Qiuping / Zheng, Jie / Li, Fengna / Duan, Yehui / Peng, Can

Animals : an open access journal from MDPI

2021 Volume 11, Issue 5

Abstract: ... supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow ...

Abstract	This study aimed to investigate the effects of dietary beta-hydroxy beta-methyl butyrate (HMB) supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow) were selected and fed a basal diet supplemented either with 0 (control), 0.13%, 0.64%, or 1.28% HMB for 60 days. Throughout the experiments, they had free access to clean drinking water and diets. Data of this study were analyzed by one-way ANOVA using the SAS 8.2 software package, followed by a Tukey's studentized range test to explore treatment effects. The results showed that compared to the control, 0.13% HMB decreased the intramuscular fat (IMF) content and increased polyunsaturated fatty acids (PUFAs) in
Language	English
Publishing date	2021-04-21
Publishing country	Switzerland
Document type	Journal Article
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani11051190
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway.

Ma, Hui-Xia / Hou, Fan / Chen, Ai-Ling / Li, Ting-Ting / Zhu, Ya-Fei / Zhao, Qi-Peng

Journal of ethnopharmacology

2020 Volume 252, Page(s) 112583

Abstract: Ethnopharmacological relevance: Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine ...

Abstract	Ethnopharmacological relevance: Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs and has been used to treat ischemic stroke for many years. However, the potential pharmacological mechanisms of MXYF remain unclear. The present research is aimed to investigate the protective effect and possible mechanisms of MXYF treatment in an in vitro model of cerebral ischemia-reperfusion injury. Materials and methods: An oxygen-glucose deprivation and reperfusion (OGD/R) model of PC12 cells was established. The effect of MXYF on the cell viability after OGD/R injury was determined using a cell counting kit (CCK-8) assay. The colorimetric method was used to determine the lactate dehydrogenase (LDH) leakage rate. The calcium concentration was determined by the chemical fluorescence method, and mitochondrial membrane potential was determined using flow cytometry. Monodansylcadaverine (MDC) staining and electron microscopic analysis were then conducted to detect autophagy after oxygen-glucose deprivation and reperfusion in PC12 cells. Immunofluorescence and western blot analyses were used to detect the expression of proteins associated with autophagy. Results: It was found that MXYF (1, 2, 4 μg/mL) could significantly increase cell viability and mitochondrial membrane potential and decrease the calcium concentration and LDH release rate in PC12 cells. After OGD/R injury in PC12 cells, the number of autophagosomes and autophagolysosome significantly increased. MXYF (4 μg/mL) inhibited the autophagy induced by OGD/R and inhibited the expression of LC3, beclin1, p-AMPK, and ULK1. In contrast, the expression of p-mTOR, p-p70s6k, and p62 was significantly enhanced. Conclusions: These findings suggest that MXYF inhibits autophagy after OGD/R-induced PC12 cell injury through the AMPK-mTOR pathway. Thus, MXYF might have therapeutic potential in treating ischemic stroke.
MeSH term(s)	AMP-Activated Protein Kinases/metabolism ; Animals ; Autophagy/drug effects ; Cell Hypoxia ; Drugs, Chinese Herbal/pharmacology ; Glucose/deficiency ; Neuroprotective Agents/pharmacology ; Oxygen ; PC12 Cells ; Rats ; Reperfusion Injury/drug therapy ; Reperfusion Injury/metabolism ; Signal Transduction/drug effects ; TOR Serine-Threonine Kinases/metabolism
Chemical Substances	Drugs, Chinese Herbal ; Mu-Xiang-You-Fang ; Neuroprotective Agents ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; mTOR protein, rat (EC 2.7.1.1) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Glucose (IY9XDZ35W2) ; Oxygen (S88TT14065)
Language	English
Publishing date	2020-01-22
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112583
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1494: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: Xiang Study: an association of breastmilk composition with maternal body mass index and infant growth during the first 3 month of life.

Peng, Xuyi / Li, Jie / Yan, Shuyuan / Chen, Juchun / Lane, Jonathan / Malard, Patrice / Liu, Feitong

Nutrition research and practice

2020 Volume 15, Issue 3, Page(s) 367–381

Abstract: Background/objectives: This study aimed to establish a mother and child cohort in the Chinese population, and investigate human breastmilk (HBM) composition and its relationship with maternal body mass index (BMI) and infant growth during the first 3 ... ...

Abstract	Background/objectives: This study aimed to establish a mother and child cohort in the Chinese population, and investigate human breastmilk (HBM) composition and its relationship with maternal body mass index (BMI) and infant growth during the first 3 mon of life. Subjects/methods: A total of 101 Chinese mother and infant pairs were included in this prospective cohort. Alterations in the milk macronutrients of Chinese mothers at 1 mon (T1), 2 mon (T2), and 3 mon (T3) lactation were analyzed. HBM fatty acid (FA) profiles were measured by gas chromatography (GC), and HBM proteomic profiling was achieved by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Results: During the first 3 mon of lactation ( Conclusion: This study provides additional evidence to the Chinese HBM database, and further knowledge of FA function. It also helps to establish future maternal strategies that support the healthy growth and development of Chinese infants.
Language	English
Publishing date	2020-10-06
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2387946-4
ISSN	2005-6168 ; 1976-1457
ISSN (online)	2005-6168
ISSN	1976-1457
DOI	10.4162/nrp.2021.15.3.367
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Mu-Xiang-You-Fang protects PC12 cells against OGD/R-induced autophagy via the AMPK/mTOR signaling pathway

Ma, Hui-xia / Chen, Ai-ling / Hou, Fan / Li, Ting-ting / Zhao, Qi-peng / Zhu, Ya-fei

Journal of ethnopharmacology. 2020 Apr. 24, v. 252

2020

Abstract: Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs ...

Abstract	Mu-Xiang-You-Fang (MXYF) is a classic prescription of Hui medicine. It is composed of five herbs and has been used to treat ischemic stroke for many years. However, the potential pharmacological mechanisms of MXYF remain unclear. The present research is aimed to investigate the protective effect and possible mechanisms of MXYF treatment in an in vitro model of cerebral ischemia-reperfusion injury.An oxygen-glucose deprivation and reperfusion (OGD/R) model of PC12 cells was established. The effect of MXYF on the cell viability after OGD/R injury was determined using a cell counting kit (CCK-8) assay. The colorimetric method was used to determine the lactate dehydrogenase (LDH) leakage rate. The calcium concentration was determined by the chemical fluorescence method, and mitochondrial membrane potential was determined using flow cytometry. Monodansylcadaverine (MDC) staining and electron microscopic analysis were then conducted to detect autophagy after oxygen-glucose deprivation and reperfusion in PC12 cells. Immunofluorescence and western blot analyses were used to detect the expression of proteins associated with autophagy.It was found that MXYF (1, 2, 4 μg/mL) could significantly increase cell viability and mitochondrial membrane potential and decrease the calcium concentration and LDH release rate in PC12 cells. After OGD/R injury in PC12 cells, the number of autophagosomes and autophagolysosome significantly increased. MXYF (4 μg/mL) inhibited the autophagy induced by OGD/R and inhibited the expression of LC3, beclin1, p-AMPK, and ULK1. In contrast, the expression of p-mTOR, p-p70s6k, and p62 was significantly enhanced.These findings suggest that MXYF inhibits autophagy after OGD/R-induced PC12 cell injury through the AMPK-mTOR pathway. Thus, MXYF might have therapeutic potential in treating ischemic stroke.
Keywords	autophagy ; calcium ; cell viability ; colorimetry ; electron microscopy ; flow cytometry ; fluorescence ; fluorescent antibody technique ; herbs ; lactate dehydrogenase ; membrane potential ; mitochondrial membrane ; models ; protective effect ; proteins ; signal transduction ; staining ; stroke ; therapeutics ; traditional medicine ; Western blotting
Language	English
Dates of publication	2020-0424
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112583
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate

Changbing Zheng / Bo Song / Qiuping Guo / Jie Zheng / Fengna Li / Yehui Duan / Can Peng

Animals, Vol 11, Iss 1190, p

2021 Volume 1190

Abstract: ... supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow ... profiling showed that in both LTM and SM of Bama Xiang mini-pigs, N -Methyl- l -glutamate was positively ...

Abstract	This study aimed to investigate the effects of dietary beta-hydroxy beta-methyl butyrate (HMB) supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow) were selected and fed a basal diet supplemented either with 0 (control), 0.13%, 0.64%, or 1.28% HMB for 60 days. Throughout the experiments, they had free access to clean drinking water and diets. Data of this study were analyzed by one-way ANOVA using the SAS 8.2 software package, followed by a Tukey’s studentized range test to explore treatment effects. The results showed that compared to the control, 0.13% HMB decreased the intramuscular fat (IMF) content and increased polyunsaturated fatty acids (PUFAs) in Longissimus thoracis muscle (LTM), and increased the n3 PUFAs in soleus muscles (SM, p < 0.05). Moreover, HMB supplementation led to alterations in the mRNA expression of genes related to lipid metabolism. Serum metabolome profiling showed that in both LTM and SM of Bama Xiang mini-pigs, N -Methyl- l -glutamate was positively correlated with SFA and nummularine A was negatively correlated with C18:3n3 PUFA ( p < 0.05). Therefore, N -Methyl- l -glutamate and nummularine A might be potential biomarkers of the HMB-supplemented group. These results suggested that dietary HMB supplementation could decrease the IMF content and increase n3 PUFAs as well as regulate the related metabolites ( N -Methyl- l -glutamate and nummularine A) in the serum of pigs.
Keywords	beta-hydroxy beta-methyl butyrate ; intramuscular fat ; n3 fatty acids ; N -Methyl- l -glutamate ; nummularine A ; pigs ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
Subject code	630
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Alterations of the Muscular Fatty Acid Composition and Serum Metabolome in Bama Xiang Mini-Pigs Exposed to Dietary Beta-Hydroxy Beta-Methyl Butyrate

Zheng, Changbing / Song, Bo / Guo, Qiuping / Zheng, Jie / Li, Fengna / Duan, Yehui / Peng, Can

Animals. 2021 Apr. 21, v. 11, no. 5

2021

Abstract	This study aimed to investigate the effects of dietary beta-hydroxy beta-methyl butyrate (HMB) supplementation on muscular lipid metabolism in Bama Xiang mini-pigs. Thirty-two piglets (8.58 ± 0.40 kg, barrow) were selected and fed a basal diet supplemented either with 0 (control), 0.13%, 0.64%, or 1.28% HMB for 60 days. Throughout the experiments, they had free access to clean drinking water and diets. Data of this study were analyzed by one-way ANOVA using the SAS 8.2 software package, followed by a Tukey’s studentized range test to explore treatment effects. The results showed that compared to the control, 0.13% HMB decreased the intramuscular fat (IMF) content and increased polyunsaturated fatty acids (PUFAs) in Longissimus thoracis muscle (LTM), and increased the n3 PUFAs in soleus muscles (SM, p < 0.05). Moreover, HMB supplementation led to alterations in the mRNA expression of genes related to lipid metabolism. Serum metabolome profiling showed that in both LTM and SM of Bama Xiang mini-pigs, N-Methyl-l-glutamate was positively correlated with SFA and nummularine A was negatively correlated with C18:3n3 PUFA (p < 0.05). Therefore, N-Methyl-l-glutamate and nummularine A might be potential biomarkers of the HMB-supplemented group. These results suggested that dietary HMB supplementation could decrease the IMF content and increase n3 PUFAs as well as regulate the related metabolites (N-Methyl-l-glutamate and nummularine A) in the serum of pigs.
Keywords	barrows ; biomarkers ; blood serum ; butyrates ; computer software ; diet ; fatty acid composition ; gene expression ; intramuscular fat ; lipid metabolism ; longissimus muscle ; metabolites ; metabolome ; miniature swine
Language	English
Dates of publication	2021-0421
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ISSN	2076-2615
DOI	10.3390/ani11051190
Database	NAL-Catalogue (AGRICOLA)

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