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Article ; Online: Does the initial surgery for necrotizing enterocolitis matter? Comparative outcomes for laparotomy vs. peritoneal drain as initial surgery for necrotizing enterocolitis in infants <1000 g birth weight.

Yanowitz, Toby Debra / Sullivan, Kevin M / Piazza, Anthony J / Brozanski, Beverly / Zaniletti, Isabella / Sharma, Jotishna / DiGeronimo, Robert / Nayak, Sujir Pritha / Wadhawan, Rajan / Reber, Kristina M / Murthy, Karna

Journal of pediatric surgery

2019 Volume 54, Issue 4, Page(s) 712–717

Abstract: Purpose: Quantify short-term outcomes associated with initial surgery [laparotomy (LAP) vs. peritoneal drain (PD)] for necrotizing enterocolitis (NEC) in extremely-low-birth-weight (ELBW) infants.: Methods: Using the Children's Hospitals Neonatal ... ...

Abstract	Purpose: Quantify short-term outcomes associated with initial surgery [laparotomy (LAP) vs. peritoneal drain (PD)] for necrotizing enterocolitis (NEC) in extremely-low-birth-weight (ELBW) infants. Methods: Using the Children's Hospitals Neonatal Database, we identified ELBW infants <32 weeks' gestation with surgical NEC (sNEC). Unadjusted and multivariable regression analyses were used to estimate the associations between LAP (or PD) and death/short bowel syndrome (SBS) and length of stay (LOS). Results: LAP was the more common initial procedure for sNEC (n = 359/528, 68%). Infants receiving LAP were older and heavier. Initial procedure was unrelated to death/SBS in both bivariate (LAP: 43% vs PD: 46%, p = 0.573) and multivariable analyses (OR = 0.89, 95% CI = 0.57, 1.38, p = 0.6). LAP was inversely related to mortality (29% vs. 41%, p < 0.007) in bivariate analysis, but not significant in multivariable analysis accounting for markers of preoperative illness severity. However, the association between LAP and SBS (14% vs. 5%, p = 0.012) remained significant in multivariable analyses (adjusted OR = 2.25, p = 0.039). LOS among survivors was unrelated to the first surgical procedure in multivariable analysis. Conclusion: ELBW infants who undergo LAP as the initial operative procedure for sNEC may be at higher risk for SBS without a clear in-hospital survival advantage or shorter hospitalization. Level of evidence: Level II.
MeSH term(s)	Birth Weight ; Databases, Factual ; Drainage/adverse effects ; Drainage/methods ; Enterocolitis, Necrotizing/mortality ; Enterocolitis, Necrotizing/surgery ; Female ; Hospitals, Pediatric ; Humans ; Infant ; Infant, Extremely Low Birth Weight ; Infant, Newborn ; Laparotomy/adverse effects ; Laparotomy/methods ; Length of Stay/statistics & numerical data ; Peritoneum/surgery ; Retrospective Studies ; Short Bowel Syndrome/epidemiology ; Short Bowel Syndrome/etiology ; Survival Rate ; Treatment Outcome
Language	English
Publishing date	2019-01-19
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	80165-3
ISSN	1531-5037 ; 0022-3468
ISSN (online)	1531-5037
ISSN	0022-3468
DOI	10.1016/j.jpedsurg.2018.12.010
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Article: Broad-spectrum G protein-coupled receptor antagonist, [D-Arg1,D-Trp5,7,9,Leu11]SP: a dual inhibitor of growth and angiogenesis in pancreatic cancer.

Guha, Sushovan / Eibl, Guido / Kisfalvi, Krisztina / Fan, Robert S / Burdick, Marie / Reber, Howard / Hines, Oscar J / Strieter, Robert / Rozengurt, Enrique

Cancer research

2005 Volume 65, Issue 7, Page(s) 2738–2745

Abstract: Substance P analogues, including [D-Arg(1),D-Trp(5,7,9),Leu(11)]SP (SPA) are broad-spectrum G ...

Abstract	Substance P analogues, including [D-Arg(1),D-Trp(5,7,9),Leu(11)]SP (SPA) are broad-spectrum G protein-coupled receptor (GPCR) antagonists that have potential antitumorigenic activities, although the mechanism(s) are not completely understood. Here, we examined the effects of SPA in ductal pancreatic cancers that express multiple GPCRs for mitogenic agonists and also produce proangiogenic chemokines. Using HPAF-II, a well-differentiated pancreatic cancer cell line as our model system, we showed that SPA inhibited multiple neuropeptide-induced Ca(2+) mobilization, DNA synthesis, and anchorage-independent growth in vitro. SPA also significantly attenuated the growth of HPAF-II tumor xenografts in nude mice beyond the treatment period. Interestingly, SPA markedly increased apoptosis but moderately decreased proliferation marker, Ki-67 in the tumor xenografts implying additional mechanism(s) for the significant growth inhibitory effect observed in vivo. HPAF-II cells express ELR(+) CXC chemokines, including IL-8/CXCL8, which bind to CXCR2 (a member of GPCR superfamily) and promote angiogenesis in multiple cancers, including pancreatic cancer. SPA inhibited CXCR2-mediated Ca(2+) mobilization and blocked specifically IL-8/CXCL8-induced angiogenesis in rat corneal micropocket assay in vivo. A salient feature of the results presented here is that SPA markedly reduced tumor-associated angiogenesis in the HPAF-II xenografts in vivo. Our results show that SPA, a broad-spectrum GPCR antagonist attenuates tumor growth in pancreatic cancer via a dual mechanism involving both the antiproliferative and antiangiogenic properties. We conclude that this novel dual-inhibitory property of SPA could be of significant therapeutic value in pancreatic cancer, when used in combination with other antiproliferative and/or antiangiogenic agents.
MeSH term(s)	Animals ; Apoptosis/drug effects ; Carcinoma, Pancreatic Ductal/blood supply ; Carcinoma, Pancreatic Ductal/drug therapy ; Carcinoma, Pancreatic Ductal/pathology ; Cell Growth Processes/drug effects ; Cornea/blood supply ; DNA, Neoplasm/biosynthesis ; Humans ; Interleukin-8/antagonists & inhibitors ; Ki-67 Antigen/biosynthesis ; Male ; Mice ; Mice, Nude ; Neovascularization, Pathologic/drug therapy ; Neovascularization, Pathologic/pathology ; Neovascularization, Physiologic/drug effects ; Pancreatic Neoplasms/blood supply ; Pancreatic Neoplasms/drug therapy ; Pancreatic Neoplasms/pathology ; Rats ; Receptors, G-Protein-Coupled/agonists ; Receptors, G-Protein-Coupled/antagonists & inhibitors ; Substance P/analogs & derivatives ; Substance P/pharmacology ; Xenograft Model Antitumor Assays
Chemical Substances	DNA, Neoplasm ; Interleukin-8 ; Ki-67 Antigen ; Receptors, G-Protein-Coupled ; substance P, Arg(1)-Trp(5,7,9)-Leu(11)- ; Substance P (33507-63-0)
Language	English
Publishing date	2005-04-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	1432-1
ISSN	1538-7445 ; 0008-5472
ISSN (online)	1538-7445
ISSN	0008-5472
DOI	10.1158/0008-5472.CAN-04-3197
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Book ; Online: Problemlösung durch Steuerungsmodelle in der Organisation und an Schnittstellen des österreichischen Gesundheitswesens

Reber, Gerhard

(Gesundheitswissenschaften Dokumente ; 2)

2005

Author's details	Univ.-Prof. Dr. Dr. Gerhard Reber, MBA
Series title	Gesundheitswissenschaften Dokumente ; 2 Gesundheitswissenschaften Dokument
Collection	Gesundheitswissenschaften Dokument
Subject code	610
Language	German
Size	1 Online-Ressource (30 Seiten)
Publisher	Oberösterreichische Gebietskrankenkasse
Publishing place	Linz
Publishing country	Austria
Document type	Book ; Online
HBZ-ID	HT019307426
DOI	10.4126/FRL01-006402201
Database	Repository for Life Sciences

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Article: G protein-coupled receptor signaling in human ductal pancreatic cancer cells: neurotensin responsiveness and mitogenic stimulation.

Ryder, N M / Guha, S / Hines, O J / Reber, H A / Rozengurt, E

Journal of cellular physiology

2001 Volume 186, Issue 1, Page(s) 53–64

Abstract: Neuropeptides and their corresponding G protein-coupled receptors (GPCRs) are increasingly ...

Abstract	Neuropeptides and their corresponding G protein-coupled receptors (GPCRs) are increasingly implicated in the autocrine/paracrine stimulation of growth of human cancers. We report that neurotensin induced rapid Ca2+ mobilization from intracellular stores followed by Ca2+ influx in five human ductal pancreatic cancer cell lines: HPAF-II, Capan-1, Capan-2, PANC-1, and MIA PaCa-2. In addition, most cell lines exhibited Ca2+ responses to multiple neuropeptides including bombesin, bradykinin, cholecystokinin, and vasopressin and to bioactive lipids, including lysophosphatidic acid (LPA), that also act via GPCRs. The well-differentiated line HPAF-II responded to at least seven independent GPCR agonists. The concentrations of neurotensin required to induce half-maximal effects (EC50) in HPAF-II and PANC-1 cells were 5 and 8nM, respectively. Digital fluorescence image analysis to measure Ca2+ responses in single cells revealed that 90% or more of HPAF-II and PANC-1 cells responded to 10nM neurotensin. Addition of neurotensin to PANC-1 cells also induced rapid and dose-dependent extracellular-regulated protein kinase (ERK-1 and ERK-2) activation and subsequently, stimulated DNA synthesis. The signaling complexity of GPCRs uncovered by these studies reveals a new aspect in the biology of human pancreatic cancer and could offer the basis for new approaches to the treatment of this disease.
MeSH term(s)	Bombesin/pharmacology ; Calcium/physiology ; Dose-Response Relationship, Drug ; Enzyme Activation/drug effects ; GTP-Binding Proteins/metabolism ; Humans ; Image Processing, Computer-Assisted ; Intracellular Membranes/metabolism ; Microscopy, Fluorescence ; Mitogen-Activated Protein Kinases/metabolism ; Mitogens/pharmacology ; Neurotensin/pharmacology ; Osmolar Concentration ; Pancreatic Ducts ; Pancreatic Neoplasms/pathology ; Pancreatic Neoplasms/physiopathology ; Receptors, Cell Surface/agonists ; Receptors, Cell Surface/antagonists & inhibitors ; Receptors, Cell Surface/physiology ; Signal Transduction/drug effects ; Signal Transduction/physiology ; Tumor Cells, Cultured
Chemical Substances	Mitogens ; Receptors, Cell Surface ; Neurotensin (39379-15-2) ; Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; GTP-Binding Proteins (EC 3.6.1.-) ; Bombesin (PX9AZU7QPK) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2001-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/1097-4652(200101)186:1<53::AID-JCP1004>3.0.CO;2-Q
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Article ; Online: Arbiters of Time: The Experience of Adults Aging with Spinal Cord Injury.

Reber, Lisa / Tan, Nasya S W / Meade, Michelle A / Forchheimer, Martin / Tate, Denise G / Clarke, Philippa

Journal of ageing and longevity

2024 Volume 3, Issue 1

Abstract: Time is a fundamental component of our lives. It is both objective, a structure outside of ourselves, and subjective, an element that is relative to the life we live and how we experience it. The disabled body must come to terms with time to understand ... ...

Abstract	Time is a fundamental component of our lives. It is both objective, a structure outside of ourselves, and subjective, an element that is relative to the life we live and how we experience it. The disabled body must come to terms with time to understand the future impact of the injury and its progression, as well as how the injury will impose a new more accelerated aging process in the body, resulting in a compressed lifespan. The body also challenges time's control of the body. This paper extends the literature on the study of time to the experience of adults aging with a spinal cord injury (SCI). Drawing from interviews conducted with adults with long-term SCI, it examines how their narratives about aging and the proactive management of their lives reflect their orientation toward and anticipation of the future. Recognizing that the spoken word often carries a multiplicity of meanings, it considers what participants' words might imply about their engagement with time. The results of this study show that the process of aging is characterized by uncertainty and the expectations of functional and health decline, requiring a sense of urgency and vigilance in the face of the uncertain course of aging with SCI. Participants understood that their lifespan was compressed due to the physiological impact of accelerated aging. Knowledge of this compression made time a scarce resource. Yet, despite it being the arbiters of their futures, so too were they the arbiters of time.
Language	English
Publishing date	2024-03-11
Publishing country	Switzerland
Document type	Journal Article
ISSN	2673-9259
ISSN (online)	2673-9259
DOI	10.3390/jal3010005
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Article: Subcutaneous

Mazzari, Giulia / Lowry, Christopher A / Langgartner, Dominik / Reber, Stefan O

Neurobiology of stress

2023 Volume 26, Page(s) 100568

Abstract: Chronic psychosocial stress is a burden of modern society and poses a clear risk factor for a plethora of somatic and affective disorders, of which most are associated with an activated immune status and chronic low-grade inflammation. Preclinical and ... ...

Abstract	Chronic psychosocial stress is a burden of modern society and poses a clear risk factor for a plethora of somatic and affective disorders, of which most are associated with an activated immune status and chronic low-grade inflammation. Preclinical and clinical studies further suggest that a failure in immunoregulation promotes an over-reaction of the inflammatory stress response and, thus, predisposes an individual to the development of stress-related disorders. Therefore, all genetic (i.e., sex) and environmental (i.e., early life adversity; ELA) factors facilitating an adult's inflammatory stress response are likely to increase their stress vulnerability. In the present study we investigated whether repeated subcutaneous (s.c.) administrations with a heat-killed preparation of
Language	English
Publishing date	2023-09-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	2816500-7
ISSN	2352-2895
ISSN	2352-2895
DOI	10.1016/j.ynstr.2023.100568
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Article ; Online: Non-Contact Face Temperature Measurement by Thermopile-Based Data Fusion.

Bhatti, Faraz / Engel, Grischan / Hampel, Joachim / Khalil, Chaimae / Reber, Andreas / Kray, Stefan / Greiner, Thomas

Sensors (Basel, Switzerland)

2023 Volume 23, Issue 18

Abstract: Thermal imaging cameras and infrared (IR) temperature measurement devices act as state-of-the-art techniques for non-contact temperature determination of the skin surface. The former is cost-intensive in many cases for widespread application, and the ... ...

Abstract	Thermal imaging cameras and infrared (IR) temperature measurement devices act as state-of-the-art techniques for non-contact temperature determination of the skin surface. The former is cost-intensive in many cases for widespread application, and the latter requires manual alignment to the measuring point. Due to this background, this paper proposes a new method for automated, non-contact, and area-specific temperature measurement of the facial skin surface. It is based on the combined use of a low-cost thermopile sensor matrix and a 2D image sensor. The temperature values as well as the 2D image data are fused using a parametric affine transformation. Based on face recognition, this allows temperature values to be assigned to selected facial regions and used specifically to determine the skin surface temperature. The advantages of the proposed method are described. It is demonstrated by means of a participant study that the temperature absolute values, which are achieved without manual alignment in an automated manner, are comparable to a commercially available IR-based forehead thermometer.
Language	English
Publishing date	2023-09-06
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23187680
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Article ; Online: Motor precision deficits in clinical high risk for psychosis.

Damme, Katherine S F / Han, Y Catherine / Han, Ziyan / Reber, Paul J / Mittal, Vijay A

European archives of psychiatry and clinical neuroscience

2023

Abstract: ... literature has often targeted a complex abnormality (e.g., gesture dysfunction, dyskinesia), or a single ... fundamental domain (e.g., accuracy) but rarely provided critical information about several of the individual ...

Abstract	Motor deficits appear prior to psychosis onset, provide insight into vulnerability as well as mechanisms that give rise to emerging illness, and are predictive of conversion. However, to date, the extant literature has often targeted a complex abnormality (e.g., gesture dysfunction, dyskinesia), or a single fundamental domain (e.g., accuracy) but rarely provided critical information about several of the individual components that make up more complex behaviors (or deficits). This preliminary study applies a novel implicit motor task to assess domains of motor accuracy, speed, recognition, and precision in individuals at clinical high risk for psychosis (CHR-p). Sixty participants (29 CHR-p; 31 healthy volunteers) completed clinical symptom interviews and a novel Serial Interception Sequence Learning (SISL) task that assessed implicit motor sequence accuracy, speed, precision, and explicit sequence recognition. These metrics were examined in multilevel models that enabled the examination of overall effects and changes in motor domains over blocks of trials and by positive/negative symptom severity. Implicit motor sequence accuracy, speed, and explicit sequence recognition were not detected as impacted in CHR-p. When compared to healthy controls, individuals at CHR-p were less precise in motor responses both overall (d = 0.91) and particularly in early blocks which normalized over later blocks. Within the CHR-p group, these effects were related to positive symptom levels (t = - 2.22, p = 0.036), such that individuals with higher symptom levels did not improve in motor precision over time (r's = 0.01-0.05, p's > 0.54). CHR-p individuals showed preliminary evidence of motor precision deficits but no other motor domain deficits, particularly in early performance that normalized with practice.
Language	English
Publishing date	2023-07-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1045583-8
ISSN	1433-8491 ; 0175-758X ; 0940-1334
ISSN (online)	1433-8491
ISSN	0175-758X ; 0940-1334
DOI	10.1007/s00406-023-01645-3
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Article ; Online: Camera-based automated monitoring of flying insects (Camfi). I. Field and computational methods.

Wallace, Jesse Rudolf Amenuvegbe / Reber, Therese Maria Joanna / Dreyer, David / Beaton, Brendan / Zeil, Jochen / Warrant, Eric

Frontiers in insect science

2023 Volume 3, Page(s) 1240400

Abstract: ... resolution (e.g. trapping and direct observation), or are expensive, technically complex, and require vehicle ... access to field sites (e.g. radar and lidar entomology). We propose a method called "Camfi" for long-term ...

Abstract	The ability to measure flying insect activity and abundance is important for ecologists, conservationists and agronomists alike. However, existing methods are laborious and produce data with low temporal resolution (e.g. trapping and direct observation), or are expensive, technically complex, and require vehicle access to field sites (e.g. radar and lidar entomology). We propose a method called "Camfi" for long-term non-invasive population monitoring and high-throughput behavioural observation of low-flying insects using images and videos obtained from wildlife cameras, which are inexpensive and simple to operate. To facilitate very large monitoring programs, we have developed and implemented a tool for automatic detection and annotation of flying insect targets in still images or video clips based on the popular Mask R-CNN framework. This tool can be trained to detect and annotate insects in a few hours, taking advantage of transfer learning. Our method will prove invaluable for ongoing efforts to understand the behaviour and ecology of declining insect populations and could also be applied to agronomy. The method is particularly suited to studies of low-flying insects in remote areas, and is suitable for very large-scale monitoring programs, or programs with relatively low budgets.
Language	English
Publishing date	2023-09-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	3123819-1
ISSN	2673-8600 ; 2673-8600
ISSN (online)	2673-8600
ISSN	2673-8600
DOI	10.3389/finsc.2023.1240400
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Article ; Online: Interference of the novel designer benzodiazepine 4'-chloro deschloroalprazolam with alprazolam analysis in toxicology and seized drug DUID casework.

Reber, Jami D / Rodgers, Corissa / Ayala, Jessica L / Miller, James / Stout, Peter / Lee, Dayong

Journal of analytical toxicology

2023 Volume 47, Issue 8, Page(s) 680–684

Abstract: ... quantification criteria (e.g., retention time) were acceptable. Additionally, the seized drug and toxicology ...

Abstract	In recent years, the emergence of the novel designer benzodiazepine 4'-chloro deschloroalprazolam has presented a new challenge for forensic laboratories by interfering with the identification and quantitation of alprazolam. As an isomer of alprazolam, 4'-chloro deschloroalprazolam has similar physicochemical properties and can be misidentified in casework samples as alprazolam without a specific method to differentiate the two analytes. Starting in late 2021, the Houston Forensic Science Center (HFSC) received toxicological and seized drug evidence indicating the presence of 4'-chloro deschloroalprazolam. An interference study was performed to supplement the laboratory's validated benzodiazepines method for toxicological samples to differentiate alprazolam from 4'-chloro deschloroalprazolam. This study showed that while the isomers could not be chromatographically resolved using the current method, they could be differentiated based on their retention times relative to the internal standard, alprazolam-d5. Based on these findings, the HFSC toxicology laboratory reports test results as "unsuitable for analysis due to an interference" if a suspected alprazolam peak elutes before the alprazolam-d5 peak, even if all identification and quantification criteria (e.g., retention time) were acceptable. Additionally, the seized drug and toxicology laboratories re-evaluated previously analyzed alprazolam-positive casework to determine if suspected 4'-chloro deschloroalprazolam had been misidentified as alprazolam. This report presents three cases: one case with toxicological evidence indicating the presence of both 4'-chloro deschloroalprazolam and alprazolam, and two cases with both seized drug material and toxicology evidence indicating the presence of 4'chloro deschloroalprazolam with no detected alprazolam.
MeSH term(s)	Benzodiazepines ; Alprazolam/analysis ; Forensic Toxicology ; Forensic Medicine ; Isomerism
Chemical Substances	Benzodiazepines (12794-10-4) ; Alprazolam (YU55MQ3IZY)
Language	English
Publishing date	2023-08-01
Publishing country	England
Document type	Journal Article
ZDB-ID	752391-9
ISSN	1945-2403 ; 0146-4760
ISSN (online)	1945-2403
ISSN	0146-4760
DOI	10.1093/jat/bkad047
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