LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 82

Search options

  1. Article ; Online: Measuring PROMIS® Well-Being in Early Childhood.

    Blackwell, Courtney K / Kallen, Michael A / Lai, Jin-Shei / Bevans, Katherine B / Wakschlag, Lauren S / Cella, David

    Journal of pediatric psychology

    2022  Volume 47, Issue 5, Page(s) 559–572

    Abstract: Objective: Expand the current Patient-Reported Outcome Measurement Information System (PROMIS®) well-being measures to early childhood (1-5 years) using best practices from PROMIS and developmental science.: Methods: Qualitative methods included ... ...

    Abstract Objective: Expand the current Patient-Reported Outcome Measurement Information System (PROMIS®) well-being measures to early childhood (1-5 years) using best practices from PROMIS and developmental science.
    Methods: Qualitative methods included expert input, literature and measure review, and parent interviews to confirm measure frameworks, item understandability, and developmental appropriateness. Quantitative methods included two waves of field testing and item response theory (IRT)-based psychometric evaluation of reliability and validity, as well as IRT centering and item calibration. Correlational analyses with other PROMIS Early Childhood (EC) Parent Report measures and known-group differences analyses by health status were conducted to evaluate construct validity. All measures were normed to the general U.S. population.
    Results: Qualitative results suggested three primary early childhood well-being domains: Positive Affect, Engagement, and Self-Regulation. Quantitative results revealed a unidimensional factor structure for Positive Affect and multidimensional factor structures for Engagement and Self-Regulation, both of which had two factors accounting for >10% of modeled variance reflecting unique unidimensional domains. This resulted in five final PROMIS EC well-being measures: Positive Affect, Engagement-Curiosity, Engagement-Persistence, Self-Regulation-Flexibility, and Self-Regulation-Frustration Tolerance. Correlations and known-groups differences analyses showed robust construct validity across a range of chronic health conditions.
    Conclusions: The new PROMIS EC Parent Report well-being measures offer clinicians and researchers a brief, efficient, and precise way to evaluate young children's well-being. All five measures include only positively valanced item content, which pushes the field to evaluate the presence of children's positive assets rather than the absence of problems.
    MeSH term(s) Child ; Child, Preschool ; Chronic Disease ; Health Status ; Humans ; Patient Reported Outcome Measures ; Psychometrics/methods ; Quality of Life ; Reproducibility of Results ; Surveys and Questionnaires
    Language English
    Publishing date 2022-05-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 625329-5
    ISSN 1465-735X ; 0146-8693
    ISSN (online) 1465-735X
    ISSN 0146-8693
    DOI 10.1093/jpepsy/jsac030
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  2. Article ; Online: Estimating acoustic cue rates in bowhead whales, Balaena mysticetus, during their fall migration through the Alaskan Beaufort Sea.

    Blackwell, Susanna B / Thode, Aaron M / Conrad, Alexander S / Ferguson, Megan C / Berchok, Catherine L / Stafford, Kathleen M / Marques, Tiago A / Kim, Katherine H

    The Journal of the Acoustical Society of America

    2021  Volume 149, Issue 5, Page(s) 3611

    Abstract: Eight years of passive acoustic data (2007-2014) from the Beaufort Sea were used to estimate the mean cue rate (calling rate) of individual bowhead whales (Balaena mysticetus) during their fall migration along the North Slope of Alaska. Calls detected on ...

    Abstract Eight years of passive acoustic data (2007-2014) from the Beaufort Sea were used to estimate the mean cue rate (calling rate) of individual bowhead whales (Balaena mysticetus) during their fall migration along the North Slope of Alaska. Calls detected on directional acoustic recorders (DASARs) were triangulated to provide estimates of locations at times of call production, which were then translated into call densities (calls/h/km
    MeSH term(s) Acoustics ; Alaska ; Animals ; Bowhead Whale ; Cues ; Seasons
    Language English
    Publishing date 2021-07-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 219231-7
    ISSN 1520-8524 ; 0001-4966
    ISSN (online) 1520-8524
    ISSN 0001-4966
    DOI 10.1121/10.0005043
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article ; Online: LNK/SH2B3 loss of function increases susceptibility to murine and human atrial fibrillation.

    Murphy, Matthew B / Yang, Zhenjiang / Subati, Tuerdi / Farber-Eger, Eric / Kim, Kyungsoo / Blackwell, Daniel J / Fleming, Matthew R / Stark, Joshua M / Van Amburg, Joseph C / Woodall, Kaylen K / Van Beusecum, Justin P / Agrawal, Vineet / Smart, Charles D / Pitzer, Ashley / Atkinson, James B / Fogo, Agnes B / Bastarache, Julie A / Kirabo, Annet / Wells, Quinn S /
    Madhur, Meena S / Barnett, Joey V / Murray, Katherine T

    Cardiovascular research

    2024  

    Abstract: Aims: The lymphocyte adaptor protein (LNK) is a negative regulator of cytokine and growth factor signaling. The rs3184504 variant in SH2B3 reduces LNK function and is linked to cardiovascular, inflammatory, and hematologic disorders including stroke. In ...

    Abstract Aims: The lymphocyte adaptor protein (LNK) is a negative regulator of cytokine and growth factor signaling. The rs3184504 variant in SH2B3 reduces LNK function and is linked to cardiovascular, inflammatory, and hematologic disorders including stroke. In mice, deletion of Lnk causes inflammation and oxidative stress. We hypothesized that Lnk-/- mice are susceptible to atrial fibrillation (AF) and that rs3184504 is associated with AF and AF-related stroke in humans. During inflammation, reactive lipid dicarbonyls are a major component of oxidative injury, and we further hypothesized that these mediators are critical drivers of the AF substrate in Lnk-/- mice.
    Methods and results: Lnk-/- or wild-type (WT) mice were treated with vehicle or 2-hydroxybenzylamine (2-HOBA), a dicarbonyl scavenger, for 3 months. Compared to WT, Lnk-/- mice displayed increased AF duration that was prevented by 2-HOBA. In the Lnk-/- atria, action potentials were prolonged with reduced transient outward K+ current, increased late Na+ current, and reduced peak Na+ current, proarrhythmic effects that were inhibited by 2-HOBA. Mitochondrial dysfunction, especially for complex I, was evident in Lnk-/- atria, while scavenging lipid dicarbonyls prevented this abnormality. Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were elevated in Lnk-/- plasma and atrial tissue, respectively, both of which caused electrical and bioenergetic remodeling in vitro. Inhibition of soluble TNF-α prevented electrical remodeling and AF susceptibility, while IL-1β inhibition improved mitochondrial respiration but had no effect on AF susceptibility. In a large database of genotyped patients, rs3184504 was associated with AF, as well as AF-related stroke.
    Conclusions: These findings identify a novel role for LNK in the pathophysiology of AF in both experimental mice and in humans. Moreover, reactive lipid dicarbonyls are critical to the inflammatory AF substrate in Lnk-/- mice and mediate the proarrhythmic effects of pro-inflammatory cytokines, primarily through electrical remodeling.
    Language English
    Publishing date 2024-02-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 80340-6
    ISSN 1755-3245 ; 0008-6363
    ISSN (online) 1755-3245
    ISSN 0008-6363
    DOI 10.1093/cvr/cvae036
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  4. Article: Weekly and Daily Tooth Brushing by Care Staff Reduces Gingivitis and Calculus in Racing Greyhounds.

    Rooney, Nicola J / Wonham, Katharine L / McIndoe, Katherine S / Casey, Rachel A / Blackwell, Emily J / Browne, William J

    Animals : an open access journal from MDPI

    2021  Volume 11, Issue 7

    Abstract: Periodontal disease is one of the most common conditions affecting dogs worldwide and is reported to be particularly prevalent in racing greyhounds. A range of potential risk factors have been hypothesised. Previous research has suggested that regular ... ...

    Abstract Periodontal disease is one of the most common conditions affecting dogs worldwide and is reported to be particularly prevalent in racing greyhounds. A range of potential risk factors have been hypothesised. Previous research has suggested that regular tooth brushing can reduce both calculus and gingivitis, but the frequency required is unclear. Here, we report a controlled blinded in situ trial, in which kennel staff brushed 160 racing greyhounds' teeth (living at six kennel establishments), either weekly, daily or never over a two-month period. All of the visible teeth were scored for calculus and gingivitis, using previously validated scales. We calculated average scores for each of the three teeth groups and overall whole mouth scores, averaging the teeth groups. Changes were compared to the baseline. After two months, the total calculus scores (controlling for baseline) were significantly different in the three treatment groups, (F
    Language English
    Publishing date 2021-06-23
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11071869
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: Crowdsourcing medical education.

    Blackwell, Katherine A / Travis, Michael J / Arbuckle, Melissa R / Ross, David A

    Medical education

    2016  Volume 50, Issue 5, Page(s) 576

    MeSH term(s) Crowdsourcing/methods ; Curriculum ; Education, Medical/methods ; Humans ; Neurosciences/education
    Language English
    Publishing date 2016-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 195274-2
    ISSN 1365-2923 ; 0308-0110
    ISSN (online) 1365-2923
    ISSN 0308-0110
    DOI 10.1111/medu.13010
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article ; Online: Weekly and Daily Tooth Brushing by Care Staff Reduces Gingivitis and Calculus in Racing Greyhounds

    Nicola J. Rooney / Katharine L. Wonham / Katherine S. McIndoe / Rachel A. Casey / Emily J. Blackwell / William J. Browne

    Animals, Vol 11, Iss 1869, p

    2021  Volume 1869

    Abstract: Periodontal disease is one of the most common conditions affecting dogs worldwide and is reported to be particularly prevalent in racing greyhounds. A range of potential risk factors have been hypothesised. Previous research has suggested that regular ... ...

    Abstract Periodontal disease is one of the most common conditions affecting dogs worldwide and is reported to be particularly prevalent in racing greyhounds. A range of potential risk factors have been hypothesised. Previous research has suggested that regular tooth brushing can reduce both calculus and gingivitis, but the frequency required is unclear. Here, we report a controlled blinded in situ trial, in which kennel staff brushed 160 racing greyhounds’ teeth (living at six kennel establishments), either weekly, daily or never over a two-month period. All of the visible teeth were scored for calculus and gingivitis, using previously validated scales. We calculated average scores for each of the three teeth groups and overall whole mouth scores, averaging the teeth groups. Changes were compared to the baseline. After two months, the total calculus scores (controlling for baseline) were significantly different in the three treatment groups, (F (2,129) = 10.76, p < 0.001) with both weekly and daily brushing resulting in significant reductions. Gingivitis was also significantly different between groups (F (2,128) = 4.57, p = 0.012), but in this case, only daily brushing resulted in a significant reduction. Although the dogs in different kennels varied significantly in their levels of both calculus (F (5,129) = 8.64, p < 0.001) and gingivitis (F (5,128) = 3.51 p = 0.005), the intervention was similarly effective in all of the establishments. The teeth groups varied, and the incisors were not significantly affected by the treatment. Since the trainers implementing the routine, reported a minimal time commitment and positive experiences, we suggest that daily brushing is recommended for racing greyhounds, and that any instructions or demonstrations should include attention to all teeth groups including the incisors. Similar trials need to be conducted with retired greyhounds since these have been shown to present particularly high levels of periodontal disease.
    Keywords teeth ; greyhound ; intervention ; brushing ; calculus ; gingivitis ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 630
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    More links

    Kategorien

  7. Article: A comparison of three methods for estimating call densities of migrating bowhead whales using passive acoustic monitoring

    Oedekoven, Cornelia S. / Marques, Tiago A. / Harris, Danielle / Thomas, Len / Thode, Aaron M. / Blackwell, Susanna B. / Conrad, Alexander S. / Kim, Katherine H.

    Environmental and ecological statistics. 2022 Mar., v. 29, no. 1

    2022  

    Abstract: Various methods for estimating animal density from visual data, including distance sampling (DS) and spatially explicit capture-recapture (SECR), have recently been adapted for estimating call density using passive acoustic monitoring (PAM) data, e.g., ... ...

    Abstract Various methods for estimating animal density from visual data, including distance sampling (DS) and spatially explicit capture-recapture (SECR), have recently been adapted for estimating call density using passive acoustic monitoring (PAM) data, e.g., recordings of animal calls. Here we summarize three methods available for passive acoustic density estimation: plot sampling, DS, and SECR. The first two require distances from the sensors to calling animals (which are obtained by triangulating calls matched among sensors), but SECR only requires matching (not localizing) calls among sensors. We compare via simulation what biases can arise when assumptions underlying these methods are violated. We use insights gleaned from the simulation to compare the performance of the methods when applied to a case study: bowhead whale call data collected from arrays of directional acoustic sensors at five sites in the Beaufort Sea during the fall migration 2007–2014. Call detections were manually extracted from the recordings by human observers simultaneously scanning spectrograms of recordings from a given site. The large discrepancies between estimates derived using SECR and the other two methods were likely caused primarily by the manual detection procedure leading to non-independent detections among sensors, while errors in estimated distances between detected calls and sensors also contributed to the observed patterns. Our study is among the first to provide a direct comparison of the three methods applied to PAM data and highlights the importance that all assumptions of an analysis method need to be met for correct inference.
    Keywords Balaena mysticetus ; acoustics ; animal density ; case studies ; humans ; mark-recapture studies ; statistics ; Beaufort Sea
    Language English
    Dates of publication 2022-03
    Size p. 101-125.
    Publishing place Springer US
    Document type Article
    ZDB-ID 2000906-9
    ISSN 1573-3009 ; 1352-8505
    ISSN (online) 1573-3009
    ISSN 1352-8505
    DOI 10.1007/s10651-021-00506-3
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

  8. Article ; Online: Myeloid Cell Derived IL1β Contributes to Pulmonary Hypertension in HFpEF.

    Agrawal, Vineet / Kropski, Jonathan A / Gokey, Jason J / Kobeck, Elizabeth / Murphy, Matthew B / Murray, Katherine T / Fortune, Niki L / Moore, Christy S / Meoli, David F / Monahan, Ken / Ru Su, Yan / Blackwell, Thomas / Gupta, Deepak K / Talati, Megha H / Gladson, Santhi / Carrier, Erica J / West, James D / Hemnes, Anna R

    Circulation research

    2023  Volume 133, Issue 11, Page(s) 885–898

    Abstract: Background: Pulmonary hypertension (PH) in heart failure with preserved ejection fraction (HFpEF) is a common and highly morbid syndrome, but mechanisms driving PH-HFpEF are poorly understood. We sought to determine whether a well-accepted murine model ... ...

    Abstract Background: Pulmonary hypertension (PH) in heart failure with preserved ejection fraction (HFpEF) is a common and highly morbid syndrome, but mechanisms driving PH-HFpEF are poorly understood. We sought to determine whether a well-accepted murine model of HFpEF also displays features of PH, and we sought to identify pathways that might drive early remodeling of the pulmonary vasculature in HFpEF.
    Methods: Eight-week-old male and female C57BL/6J mice received either N
    Results: N
    Conclusions: We report a novel model for the study of PH and right heart remodeling in HFpEF, and we identify myeloid cell-derived IL-1β as an important contributor to PH in HFpEF.
    MeSH term(s) Animals ; Female ; Humans ; Male ; Mice ; Clodronic Acid ; Heart Failure/metabolism ; Hypertension, Pulmonary/etiology ; Interleukin-1beta ; Mice, Inbred C57BL ; Myeloid Cells/metabolism ; Stroke Volume/physiology
    Chemical Substances Clodronic Acid (0813BZ6866) ; Interleukin-1beta ; IL1B protein, mouse
    Language English
    Publishing date 2023-11-06
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 80100-8
    ISSN 1524-4571 ; 0009-7330 ; 0931-6876
    ISSN (online) 1524-4571
    ISSN 0009-7330 ; 0931-6876
    DOI 10.1161/CIRCRESAHA.123.323119
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article ; Online: Multiplatform Single-Cell Analysis Identifies Immune Cell Types Enhanced in Pulmonary Fibrosis.

    Serezani, Ana P M / Pascoalino, Bruno D / Bazzano, Julia M R / Vowell, Katherine N / Tanjore, Harikrishna / Taylor, Chase J / Calvi, Carla L / McCall, A Scott / Bacchetta, Matthew D / Shaver, Ciara M / Ware, Lorraine B / Salisbury, Margaret L / Banovich, Nicholas E / Kendall, Peggy L / Kropski, Jonathan A / Blackwell, Timothy S

    American journal of respiratory cell and molecular biology

    2022  Volume 67, Issue 1, Page(s) 50–60

    Abstract: Immune cells have been implicated in idiopathic pulmonary fibrosis (IPF), but the phenotypes and effector mechanisms of these cells remain incompletely characterized. We performed mass cytometry to quantify immune cell subsets in lungs of 12 patients ... ...

    Abstract Immune cells have been implicated in idiopathic pulmonary fibrosis (IPF), but the phenotypes and effector mechanisms of these cells remain incompletely characterized. We performed mass cytometry to quantify immune cell subsets in lungs of 12 patients with IPF and 15 organ donors without chronic lung disease and used existing single-cell RNA-sequencing data to investigate transcriptional profiles of immune cells overrepresented in IPF. Among myeloid cells, we found increased numbers of alveolar macrophages (AMØs) and dendritic cells (DCs) in IPF, as well as a subset of monocyte-derived DCs. In contrast, monocyte-like cells and interstitial macrophages were reduced in IPF. Transcriptomic profiling identified an enrichment for IFN-γ response pathways in AMØs and DCs from IPF, as well as antigen processing in DCs and phagocytosis in AMØs. Among T cells, we identified three subsets of memory T cells that were increased in IPF, including CD4
    MeSH term(s) Gene Expression Profiling ; Humans ; Idiopathic Pulmonary Fibrosis/pathology ; Lung/pathology ; Macrophages, Alveolar/metabolism ; Single-Cell Analysis
    Language English
    Publishing date 2022-04-20
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1025960-0
    ISSN 1535-4989 ; 1044-1549
    ISSN (online) 1535-4989
    ISSN 1044-1549
    DOI 10.1165/rcmb.2021-0418OC
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article: Myeloid Cell Derived IL1β Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction.

    Agrawal, Vineet / Kropski, Jonathan A / Gokey, Jason J / Kobeck, Elizabeth / Murphy, Matthew / Murray, Katherine T / Fortune, Niki L / Moore, Christy S / Meoli, David F / Monahan, Ken / Su, Yan Ru / Blackwell, Thomas / Gupta, Deepak K / Talati, Megha H / Gladson, Santhi / Carrier, Erica J / West, James D / Hemnes, Anna R

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Background: Pulmonary hypertension (PH) in heart failure with preserved ejection fraction (HFpEF) is a common and highly morbid syndrome, but mechanisms driving PH-HFpEF are not well understood. We sought to determine whether a well-accepted murine ... ...

    Abstract Background: Pulmonary hypertension (PH) in heart failure with preserved ejection fraction (HFpEF) is a common and highly morbid syndrome, but mechanisms driving PH-HFpEF are not well understood. We sought to determine whether a well-accepted murine model of HFpEF also displays features of PH in HFpEF, and we sought to identify pathways that might drive early remodeling of the pulmonary vasculature in HFpEF.
    Methods: Eight week old male and female C57/BL6J mice were given either L-NAME and high fat diet (HFD) or control water/diet for 2,5, and 12 weeks. Bulk RNA sequencing and single cell RNA sequencing was performed to identify early and cell-specific pathways that might regulate pulmonary vascular remodeling in PH-HFpEF. Finally, clodronate liposome and IL1β antibody treatments were utilized to deplete macrophages or IL1β, respectively, to assess their impact on pulmonary vascular remodeling in HFpEF.
    Results: Mice given L-NAME/HFD developed PH, small vessel muscularization, and right heart dysfunction after 2 weeks of treatment. Inflammation-related gene ontologies were over-represented in bulk RNA sequencing analysis of whole lungs, with an increase in CD68+ cells in both murine and human PH-HFpEF lungs. Cytokine profiling of mouse lung and plasma showed an increase in IL1β, which was confirmed in plasma from patients with HFpEF. Single cell sequencing of mouse lungs also showed an increase in M1-like, pro-inflammatory populations of Ccr2+ monocytes and macrophages, and transcript expression of IL1β was primarily restricted to myeloid-type cells. Finally, clodronate liposome treatment prevented the development of PH in L-NAME/HFD treated mice, and IL1β antibody treatment also attenuated PH in L-NAME/HFD treated mice.
    Conclusions: Our study demonstrated that a well-accepted model of HFpEF recapitulates features of pulmonary vascular remodeling commonly seen in patients with HFpEF, and we identified myeloid cell derived IL1β as an important contributor to PH in HFpEF.
    Language English
    Publishing date 2023-05-18
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.18.541302
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top