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Article ; Online: Maribacter halichondriae sp. nov., isolated from the marine sponge Halichondria panicea, displays features of a sponge-associated life style.

Steiner, Leon X / Wiese, Jutta / Rahn, Tanja / Borchert, Erik / Slaby, Beate M / Hentschel, Ute

2024 Volume 117, Issue 1, Page(s) 56

Abstract: A new member of the family Flavobacteriaceae (termed ... ...

Abstract	A new member of the family Flavobacteriaceae (termed Hal144
MeSH term(s)	Animals ; Seawater ; Phosphatidylethanolamines/chemistry ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Porifera ; DNA, Bacterial/genetics ; Sequence Analysis, DNA ; Bacterial Typing Techniques ; Vitamin K 2/chemistry ; Fatty Acids/chemistry ; Flavobacteriaceae
Chemical Substances	Phosphatidylethanolamines ; RNA, Ribosomal, 16S ; DNA, Bacterial ; Vitamin K 2 (11032-49-8) ; Fatty Acids
Language	English
Publishing date	2024-03-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	214861-4
ISSN	1572-9699 ; 0003-6072
ISSN (online)	1572-9699
ISSN	0003-6072
DOI	10.1007/s10482-024-01950-4
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Article: Wedge Shaped vs Round Implants: Bone Strain During the Insertion Process.

Steiner, Constanze / Karl, Matthias / Grobecker-Karl, Tanja

The Journal of oral implantology

2022 Volume 48, Issue 6, Page(s) 557–561

Abstract: A novel implant system resembling the shape of a wedge and employing piezosurgery for implant bed preparation has been introduced with the aim of solving the problem of horizontal bone deficiency. This in vitro study compared emerging bone strain during ... ...

Abstract	A novel implant system resembling the shape of a wedge and employing piezosurgery for implant bed preparation has been introduced with the aim of solving the problem of horizontal bone deficiency. This in vitro study compared emerging bone strain during insertion of a conventionally round implant vs the wedge implant. Adhering to the manufacturers' protocols, implant surgery was performed in polyurethane foam blocks equipped with strain gauges attached to the buccal and occlusal surfaces. Five implants per group were placed while strain development during insertion was recorded. Primary implant stability was determined using resonance frequency analysis. Statistical analysis was based on Welch's 2-sample tests (α = 0.05). In general, greater strain development was found on the buccal aspect of bone compared to the occlusal aspect with an overall range between -724 μm/m and 9132 μm/m. A stepwise increase in strain development was seen in the wedge implants while, in the round implants, a continuous increase in strain development was recorded. Absolute strain development on the buccal aspect of bone was significantly greater in wedge implants (P = .0137) while, on the occlusal aspect, significantly lower strain development was seen for wedge implants (P = .0012). Primary stability of wedge implants was significantly lower compared to round implants (P = .0005). Wedge implants differ from round implants with respect to the insertion process characterized by a stepwise increase in bone deformation. High strain development in buccal bone may constitute a risk factor for bone resorption and should be avoided by reducing the degree of underpreparation of the implant site.
MeSH term(s)	Humans ; Dental Implants ; Dental Implantation, Endosseous/methods ; Bone and Bones ; Zygoma ; Bone Resorption ; Torque
Chemical Substances	Dental Implants
Language	English
Publishing date	2022-04-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	448462-9
ISSN	1548-1336 ; 0160-6972
ISSN (online)	1548-1336
ISSN	0160-6972
DOI	10.1563/aaid-joi-D-21-00135
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Article ; Online: Quantum Chemical Microsolvation by Automated Water Placement.

Steiner, Miguel / Holzknecht, Tanja / Schauperl, Michael / Podewitz, Maren

Molecules (Basel, Switzerland)

2021 Volume 26, Issue 6

Abstract: We developed a quantitative approach to quantum chemical microsolvation. Key in our methodology is the automatic placement of individual solvent molecules based on the free energy solvation thermodynamics derived from molecular dynamics (MD) simulations ... ...

Abstract	We developed a quantitative approach to quantum chemical microsolvation. Key in our methodology is the automatic placement of individual solvent molecules based on the free energy solvation thermodynamics derived from molecular dynamics (MD) simulations and grid inhomogeneous solvation theory (GIST). This protocol enabled us to rigorously define the number, position, and orientation of individual solvent molecules and to determine their interaction with the solute based on physical quantities. The generated solute-solvent clusters served as an input for subsequent quantum chemical investigations. We showcased the applicability, scope, and limitations of this computational approach for a number of small molecules, including urea, 2-aminobenzothiazole, (+)-
Language	English
Publishing date	2021-03-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26061793
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Article ; Online: Quantum Chemical Microsolvation by Automated Water Placement

Miguel Steiner / Tanja Holzknecht / Michael Schauperl / Maren Podewitz

Molecules, Vol 26, Iss 1793, p

2021 Volume 1793

Abstract	We developed a quantitative approach to quantum chemical microsolvation. Key in our methodology is the automatic placement of individual solvent molecules based on the free energy solvation thermodynamics derived from molecular dynamics (MD) simulations and grid inhomogeneous solvation theory (GIST). This protocol enabled us to rigorously define the number, position, and orientation of individual solvent molecules and to determine their interaction with the solute based on physical quantities. The generated solute–solvent clusters served as an input for subsequent quantum chemical investigations. We showcased the applicability, scope, and limitations of this computational approach for a number of small molecules, including urea, 2-aminobenzothiazole, (+)- syn -benzotriborneol, benzoic acid, and helicene. Our results show excellent agreement with the available ab initio molecular dynamics data and experimental results.
Keywords	microsolvation ; implicit-explicit salvation ; density functional theory ; molecular dynamics ; grid inhomogeneous solvation theory ; bulk phase ; Organic chemistry ; QD241-441
Subject code	541
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Maribacter halichondriae sp. nov., isolated from the marine sponge Halichondria panicea, displays features of a sponge-associated life style

Steiner, Leon X. / Wiese, Jutta / Rahn, Tanja / Borchert, Erik / Slaby, Beate M. / Hentschel, Ute

2024

Abstract: A new member of the family Flavobacteriaceae (termed Hal144T) was isolated from the marine breadcrumb sponge Halichondria panicea. Sponge material was collected in 2018 at Schilksee which is located in the Kiel Fjord (Baltic Sea, Germany). Phylogenetic ... ...

Abstract	A new member of the family Flavobacteriaceae (termed Hal144T) was isolated from the marine breadcrumb sponge Halichondria panicea. Sponge material was collected in 2018 at Schilksee which is located in the Kiel Fjord (Baltic Sea, Germany). Phylogenetic analysis of the full-length Hal144T 16S rRNA gene sequence revealed similarities from 94.3 to 96.6% to the nearest type strains of the genus Maribacter. The phylogenetic tree of the 16S rRNA gene sequences depicted a cluster of strain Hal144T with its closest relatives Maribacter aestuarii GY20T (96.6%) and Maribacter thermophilus HT7-2T (96.3%). Genome phylogeny showed that Maribacter halichondriae Hal144T branched from a cluster consisting of Maribacter arenosus, Maribacter luteus, and Maribacter polysiphoniae. Genome comparisons of strain Maribacter halichondriae Hal144T with Maribacter sp. type strains exhibited average nucleotide identities in the range of 75–76% and digital DNA-DNA hybridisation values in the range of 13.1–13.4%. Compared to the next related type strains, strain Hal144T revealed unique genomic features such as phosphoenolpyruvate-dependent phosphotransferase system pathway, serine-glyoxylate cycle, lipid A 3-O-deacylase, 3-hexulose-6-phosphate synthase, enrichment of pseudogenes and of genes involved in cell wall and envelope biogenesis, indicating an adaptation to the host. Strain Hal144T was determined to be Gram-negative, mesophilic, strictly aerobic, flexirubin positive, resistant to aminoglycoside antibiotics, and able to utilize N-acetyl-β-D-glucosamine. Optimal growth occurred at 25–30 °C, within a salinity range of 2–6% sea salt, and a pH range between 5 and 8. The major fatty acids identified were C17:0 3-OH, iso-C15:0, and iso-C15:1 G. The DNA G + C content of strain Hal144T was 41.4 mol%. Based on the polyphasic approach, strain Hal144T represents a novel species of the genus Maribacter, and we propose the name Maribacter halichondriae sp. nov. The type strain is Hal144T (= DSM 114563T = LMG 32744T).
Subject code	572
Language	English
Publishing date	2024-03-15
Publisher	Springer
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Insertion torque/time integral as a measure of primary implant stability.

Grobecker-Karl, Tanja / Karl, Matthias / Steiner, Constanze

Biomedizinische Technik. Biomedical engineering

2020

Abstract: The goal of this in vitro study was to determine the insertion torque/time integral for three implant systems. Bone level implants (n = 10; BLT - Straumann Bone Level Tapered 4.1 mm × 12 mm, V3 - MIS V3 3.9 mm × 11.5 mm, ASTRA - Dentsply-Sirona ASTRA TX ... ...

Abstract	The goal of this in vitro study was to determine the insertion torque/time integral for three implant systems. Bone level implants (n = 10; BLT - Straumann Bone Level Tapered 4.1 mm × 12 mm, V3 - MIS V3 3.9 mm × 11.5 mm, ASTRA - Dentsply-Sirona ASTRA TX 4.0 mm × 13 mm) were placed in polyurethane foam material consisting of a trabecular and a cortical layer applying protocols for medium quality bone. Besides measuring maximum insertion torque and primary implant stability using resonance frequency analysis (RFA), torque time curves recorded during insertion were used for calculating insertion torque/time integrals. Statistical analysis was based on ANOVA, Tukey's honest differences test and Pearson product moment correlation (α = 0.05). Significantly greater mean maximum insertion torque (59.9 ± 4.94 Ncm) and mean maximum insertion torque/time integral (961.64 ± 54.07 Ncm∗s) were recorded for BLT implants (p < 0.01). V3 showed significantly higher mean maximum insertion torque as compared to ASTRA (p < 0.01), but significantly lower insertion torque/time integral (p < 0.01). Primary implant stability did not differ significantly among groups. Only a single weak (r = 0.61) but significant correlation could be established between maximum insertion torque and insertion torque/time integral (p < 0.01) when all data from all three implant groups were pooled. Implant design (length, thread pitch) seems to affect insertion torque/time integral more than maximum insertion torque.
Language	English
Publishing date	2020-06-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	120817-2
ISSN	1862-278X ; 0013-5585
ISSN (online)	1862-278X
ISSN	0013-5585
DOI	10.1515/bmt-2020-0039
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Article ; Online: Insertion and Loading Characteristics of Three Different Bone-Level Implants.

Steiner, Constanze / Karl, Matthias / Grobecker-Karl, Tanja

The International journal of oral & maxillofacial implants

2020 Volume 35, Issue 3, Page(s) 560–565

Abstract: Purpose: While primary stability still constitutes an important factor for implant success, high levels of insertion torque resulting from bone compression are controversial and may constitute a co-factor in peri-implant bone loss.: Materials and ... ...

Abstract	Purpose: While primary stability still constitutes an important factor for implant success, high levels of insertion torque resulting from bone compression are controversial and may constitute a co-factor in peri-implant bone loss. Materials and methods: Adhering to the manufacturers' protocols for medium-quality bone, implant surgery was performed in polyurethane foam blocks equipped with strain gauges attached to the buccal aspect. Following insertion and attachment of provisional abutments, oblique loading was performed. The following parameters were recorded for three different implant types (Straumann Bone Level Tapered [BLT], MIS V3 [V3], Dentsply Sirona ASTRA TX [ASTRA]): maximum insertion and removal torque, maximum strain during insertion and loading, and implant stability before and after loading (resonance frequency analysis [RFA]). Statistical analysis was based on analysis of variance (ANOVA), Tukey honest significant difference test, and Pearson's product moment correlation (α = .05). Results: Maximum insertion torque (59.9 ± 4.94 Ncm) was recorded for BLT followed by V3 and ASTRA (P < .01 for all comparisons). Maximum removal torque (43.7 ± 9.69 Ncm) was also recorded for BLT, but the pairwise comparisons reached significance only for BLT vs ASTRA (P < .01) and V3 vs BLT (P = .03). Implant stability differed among groups only after loading, where the pairwise comparison between BLT and ASTRA reached significance (P = .02). Maximum strain during insertion was caused by BLT reaching 19,482.62 <m/m, whereas ASTRA implants only caused 6,169.84 <m/m. Strain development during insertion differed significantly among groups (P < .05 for all comparisons). Maximum strain during loading was observed in V3 (646.44 ± 204 μm/m), while only a nonsignificant difference existed between ASTRA and BLT. Insertion torque correlated significantly with strain development (r = 0.68; P < .01), implant stability after loading (r = 0.46; P = .01), and removal torque (r = 0.54; P < .01). Also, implant stability after loading correlated with removal torque (r = 0.86; P < .01).<br />Conclusion: Different implant designs and surgical protocols result in varying levels of bone compression. Implants with a triangular shape do not seem to solve this problem.
MeSH term(s)	Dental Implantation, Endosseous ; Dental Implants ; Torque
Chemical Substances	Dental Implants
Language	English
Publishing date	2020-05-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	632880-5
ISSN	1942-4434 ; 0882-2786
ISSN (online)	1942-4434
ISSN	0882-2786
DOI	10.11607/jomi.7770
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Zs.A 2179: Show issues

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Article ; Online: Polysulfides derived from the hydrogen sulfide and persulfide donor P* inhibit IL-1β-mediated inducible nitric oxide synthase signaling in ATDC5 cells: are CCAAT/enhancer-binding proteins β and δ involved in the anti-inflammatory effects of hydrogen sulfide and polysulfides?

Trummer, Modesta / Galardon, Erwan / Mayer, Bernd / Steiner, Günter / Stamm, Tanja / Kloesch, Burkhard

Nitric oxide : biology and chemistry

2022 Volume 129, Page(s) 41–52

Abstract: Hydrogen sulfide (H ... 2 ... S) emerged as an essential signaling molecule exerting beneficial effects in various cardiovascular, neurodegenerative, or musculoskeletal diseases with an inflammatory component, such as osteoarthritis. These protective ... ...

Abstract	Hydrogen sulfide (H<sub>2</sub>S) emerged as an essential signaling molecule exerting beneficial effects in various cardiovascular, neurodegenerative, or musculoskeletal diseases with an inflammatory component, such as osteoarthritis. These protective effects were initially attributed to protein S-sulfhydration, a posttranslational modification of reactive cysteine residues. However, recent studies suggest that polysulfides and not H<sub>2</sub>S are responsible for S-sulfhydration. To distinguish between H<sub>2</sub>S and polysulfide-mediated effects in this study, we used the slow-releasing H<sub>2</sub>S and persulfide donor P, which can be decomposed into polysulfides. The effects of P on IL-1β-induced inducible nitric oxide synthase (iNOS), a pro-inflammatory mediator in osteoarthritis, were determined by nitrite measurement, qPCR, and Western blotting in the murine chondrocyte-like cell line ATDC5. Decomposed P* significantly reduced IL-1β-induced iNOS signaling via polysulfides, independently of H<sub>2</sub>S. In line with this, the fast-releasing H<sub>2</sub>S donor NaHS was ineffective. In RAW 264.7 macrophages, similar results were obtained. P-derived polysulfides further diminished IL-1β-induced CCAAT/enhancer-binding protein (C/EBP) β and δ expression in ATDC5 cells, which might play a critical role in P-mediated iNOS decline. In conclusion, our data support the view that polysulfides are essential signaling molecules as well as potential mediators of H<sub>2</sub>S signaling. Moreover, we propose that C/EBPβ/δ might be a novel target involved in H<sub>2</sub>S and polysulfide-mediated anti-inflammatory signaling.
MeSH term(s)	Mice ; Animals ; Hydrogen Sulfide/pharmacology ; Hydrogen Sulfide/metabolism ; Nitric Oxide Synthase Type II/metabolism ; Sulfides/pharmacology ; Sulfides/metabolism ; Anti-Inflammatory Agents ; Osteoarthritis ; Nitric Oxide/metabolism
Chemical Substances	Hydrogen Sulfide (YY9FVM7NSN) ; persulfides ; polysulfide (9080-49-3) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Sulfides ; Anti-Inflammatory Agents ; Nitric Oxide (31C4KY9ESH)
Language	English
Publishing date	2022-10-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1362794-6
ISSN	1089-8611 ; 1089-8603
ISSN (online)	1089-8611
ISSN	1089-8603
DOI	10.1016/j.niox.2022.09.005
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Article: Primär- versus sekundärverblockte Versorgung zahnloser Patienten

Grobecker-Karl, Tanja / Steiner, Constanze / Karl, Matthias

ZWR - Das Deutsche Zahnärzteblatt

2020 Volume 129, Issue 04, Page(s) 137–145

Language	German
Publishing date	2020-04-01
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	210131-2
ISSN	1439-9148 ; 0044-166X
ISSN (online)	1439-9148
ISSN	0044-166X
DOI	10.1055/a-1108-6180
Database	Thieme publisher's database

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Article ; Online: Thyrotropin Receptor Antagonism by a Novel Small Molecule: Preclinical

Kahaly, George J / Steiner, Lisa / van der Lee, Miranda M C / van Achterberg, Tanja A E / Arends, Roel J / Karstens, Willem F J / Weirauch, Tobias / Henseling, Jil / Frommer, Lara / Wolf, Jan / George, Augustine

Thyroid : official journal of the American Thyroid Association

2023 Volume 33, Issue 6, Page(s) 732–742

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Cricetinae ; Animals ; Humans ; Receptors, Thyrotropin ; Graves Ophthalmopathy/drug therapy ; Cricetulus ; CHO Cells ; Immunoglobulins, Thyroid-Stimulating ; Thyrotropin/metabolism ; Autoantibodies
Chemical Substances	Receptors, Thyrotropin ; Immunoglobulins, Thyroid-Stimulating ; Thyrotropin (9002-71-5) ; Autoantibodies
Language	English
Publishing date	2023-05-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1086044-7
ISSN	1557-9077 ; 1050-7256
ISSN (online)	1557-9077
ISSN	1050-7256
DOI	10.1089/thy.2022.0694
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