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  1. Article: Harry S. Weeks Jr., M.D., R.I.P.

    Ward, S D

    The West Virginia medical journal

    1997  Volume 93, Issue 1, Page(s) 356

    MeSH term(s) Anesthesiology/history ; History, 20th Century ; West Virginia
    Language English
    Publishing date 1997-01
    Publishing country United States
    Document type Biography ; Editorial ; Historical Article ; Portrait
    ZDB-ID 431705-1
    ISSN 0043-3284
    ISSN 0043-3284
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  2. Article: Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains.

    Bastien, Nathalie / Normand, Susan / Taylor, Tracy / Ward, Diane / Peret, Teresa C T / Boivin, Guy / Anderson, Larry J / Li, Yan

    Virus research

    2003  Volume 93, Issue 1, Page(s) 51–62

    Abstract: The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M ... variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F ... identity between the two distinct clusters The P gene showed more diversity with 81.6-85.7% amino acid ...

    Abstract The complete nucleotide sequences of the nucleoprotein (N), phosphoprotein (P), matrix protein (M), and fusion protein (F) genes of 15 Canadian human metapneumovirus (hMPV) isolates were determined. Phylogenetic analysis revealed two distinct genetic clusters, or groups for each gene with additional sequence variability within the individual groups. Comparison of the deduced amino acid sequences for the N, M and F genes of the different isolates revealed that all three genes were well conserved with 94.1-97.6% identity between the two distinct clusters The P gene showed more diversity with 81.6-85.7% amino acid identity for isolates between the two clusters, and 94.6-100% for isolates within the same cluster.
    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Canada ; DNA Primers ; Humans ; Metapneumovirus/classification ; Metapneumovirus/genetics ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; Sequence Homology, Amino Acid ; Viral Matrix Proteins/chemistry ; Viral Matrix Proteins/genetics ; Viral Proteins/genetics
    Chemical Substances DNA Primers ; Viral Matrix Proteins ; Viral Proteins
    Keywords covid19
    Language English
    Publishing date 2003-04-11
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/s0168-1702(03)00065-0
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  3. Article: Dipeptidyl(amino)peptidase IV and aminopeptidase M metabolize circulating substance P in vivo.

    Ahmad, S / Wang, L / Ward, P E

    The Journal of pharmacology and experimental therapeutics

    1992  Volume 260, Issue 3, Page(s) 1257–1261

    Abstract: ... plasma from DAP IV-positive rats metabolized substance P (SP) (5.37 +/- 0.25 nmol/min/ml) via the actions ... hydrolyzed by plasma aminopeptidase M (AmM; EC 3.4.11.2) (36.2 +/- 4.2 nmol/min/ml). In contrast, SP ...

    Abstract Recent studies have demonstrated that Fischer-344 rats from Japanese Charles River Inc. specifically lack dipeptidyl(amino)peptidase IV (DAP IV-negative; EC 3.4.14.5), whereas Fischer-344 rats from sources within the United States (DAP IV-positive) possess normal DAP IV activity. In the present study, plasma from DAP IV-positive rats metabolized substance P (SP) (5.37 +/- 0.25 nmol/min/ml) via the actions of angiotensin-converting enzyme (EC 3.4.15.1) (1.86 +/- 0.50 nmol/min/ml) and DAP IV (2.56 +/- 0.42 nmol/min/ml). DAP IV sequentially converted SP to SP[3-11] and SP[5-11]. The SP[5-11] metabolite was then rapidly hydrolyzed by plasma aminopeptidase M (AmM; EC 3.4.11.2) (36.2 +/- 4.2 nmol/min/ml). In contrast, SP metabolism by plasma from DAP IV-negative rats was less than half that of control animals (2.14 +/- 0.06 nmol/min/ml), due to a complete lack of DAP IV hydrolysis. The absence of DAP IV was not associated with any differences in angiotensin-converting enzyme-mediated hydrolysis of SP (1.45 +/- 0.11 nmol/min/ml) or AmM-mediated hydrolysis of SP[5-11] (37.1 +/- 0.9 nmol/min/ml). Consistent with this deficiency in SP metabolism, SP was more potent in vivo in stimulating salivary secretion in DAP IV-negative rats compared to DAP IV-positive animals. Potentiation was specific in that SP[5-11], an SP fragment resistant to DAP IV, was equipotent in DAP IV-negative and positive animals. SP[5-11]-induced salivary secretion was potentiated in both strains when AmM-mediated hydrolysis was inhibited by amastatin (20 nmol/min, i.v.). These data provide direct evidence for a significant role for DAP IV and AmM in the in vivo processing of SP and active SP metabolites.
    MeSH term(s) Aminopeptidases/physiology ; Animals ; CD13 Antigens ; Dipeptidyl Peptidase 4 ; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/physiology ; Female ; Male ; Peptide Fragments/metabolism ; Rats ; Substance P/metabolism
    Chemical Substances Peptide Fragments ; Substance P (33507-63-0) ; substance P (5-11) (51165-09-4) ; Aminopeptidases (EC 3.4.11.-) ; CD13 Antigens (EC 3.4.11.2) ; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases (EC 3.4.14.-) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5)
    Language English
    Publishing date 1992-03
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 3106-9
    ISSN 1521-0103 ; 0022-3565
    ISSN (online) 1521-0103
    ISSN 0022-3565
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  4. Article ; Online: Embedding research and enquiry in Australian DVM curriculum.

    Šlapeta, J / Ward, M P

    Australian veterinary journal

    2024  

    Abstract: Research and enquiry (R&E) is an integral part of veterinary training. It is a foundation of evidence-based practice. In the University of Sydney Doctor of Veterinary Medicine degree R&E culminates in a cap-stone experience in Year 3: a 'professionally ... ...

    Abstract Research and enquiry (R&E) is an integral part of veterinary training. It is a foundation of evidence-based practice. In the University of Sydney Doctor of Veterinary Medicine degree R&E culminates in a cap-stone experience in Year 3: a 'professionally focused project', a student-driven and academic supported individual research project. The project provides an authentic experience within a veterinary discipline. Students work with an academic advisor who provides guidance for developing and achieving meaningful educational and professional goals. Successful advising depends upon a shared understanding of, and commitment to, the advising process by students, advisors and the university. The R&E mission can be broadly defined as - veterinarians recognise that evidence-based approach to practice, which is based on the scientific method, leads to the generation of new knowledge that underpins the veterinary medical profession.
    Language English
    Publishing date 2024-04-23
    Publishing country England
    Document type Letter
    ZDB-ID 41542-x
    ISSN 1751-0813 ; 0005-0423
    ISSN (online) 1751-0813
    ISSN 0005-0423
    DOI 10.1111/avj.13334
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  5. Article: Differential processing of substance P and neurokinin A by plasma dipeptidyl(amino)peptidase IV, aminopeptidase M and angiotensin converting enzyme.

    Wang, L H / Ahmad, S / Benter, I F / Chow, A / Mizutani, S / Ward, P E

    Peptides

    1991  Volume 12, Issue 6, Page(s) 1357–1364

    Abstract: In addition to plasma metabolism of substance P (SP) by angiotensin converting enzyme (ACE; EC 3.4 ... plasma aminopeptidase M (AmM; EC 3.4.11.2) (24.2-25.5 nmol/min/ml). The Km values of SP for DAP IV and ...

    Abstract In addition to plasma metabolism of substance P (SP) by angiotensin converting enzyme (ACE; EC 3.4.15.1) (less than 1.0 nmol/min/ml), the majority of SP hydrolysis by rat and human plasma was due to dipeptidyl(amino)peptidase IV (DAP IV; EC 3.4.14.5) (3.15-5.91 nmol/min/ml), which sequentially converted SP to SP(3-11) and SP(5-11). In turn, the SP(5-11) metabolite was rapidly hydrolyzed by rat and human plasma aminopeptidase M (AmM; EC 3.4.11.2) (24.2-25.5 nmol/min/ml). The Km values of SP for DAP IV and of SP(5-11) for AmM ranged from 32.7 to 123 microM. In contrast, neurokinin A (NKA) was resistant to both ACE and DAP IV but was subject to N-terminal hydrolysis by AmM (3.76-10.8 nmol/min/ml; Km = 90.7 microM). These data demonstrate differential processing of SP and NKA by specific peptidases in rat and human plasma.
    MeSH term(s) Aminopeptidases/blood ; Animals ; Anti-Bacterial Agents ; CD13 Antigens ; Captopril/pharmacology ; Dipeptidyl Peptidase 4 ; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/blood ; Humans ; In Vitro Techniques ; Kinetics ; Male ; Neurokinin A/blood ; Oligopeptides/pharmacology ; Peptide Fragments/blood ; Peptides ; Peptidyl-Dipeptidase A/blood ; Rats ; Species Specificity ; Substance P/blood
    Chemical Substances Anti-Bacterial Agents ; Oligopeptides ; Peptide Fragments ; Peptides ; Substance P (33507-63-0) ; amastatin (67655-94-1) ; Neurokinin A (86933-74-6) ; diprotin A (90614-48-5) ; Captopril (9G64RSX1XD) ; Aminopeptidases (EC 3.4.11.-) ; CD13 Antigens (EC 3.4.11.2) ; Dipeptidyl-Peptidases and Tripeptidyl-Peptidases (EC 3.4.14.-) ; Dipeptidyl Peptidase 4 (EC 3.4.14.5) ; Peptidyl-Dipeptidase A (EC 3.4.15.1)
    Language English
    Publishing date 1991-11
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 769028-9
    ISSN 1873-5169 ; 0196-9781
    ISSN (online) 1873-5169
    ISSN 0196-9781
    DOI 10.1016/0196-9781(91)90220-j
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    Zs.A 1649: Show issues Location:
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  6. Article ; Online: Results of Implementation of Amyloidosis Screening for Patients Undergoing Carpal Tunnel Release.

    Gannon, Nicholas P / Ward, Christina M

    The Journal of hand surgery

    2023  

    Abstract: Purpose: Many patients with amyloidosis undergo carpal tunnel release (CTR) before amyloidosis diagnosis and before developing cardiac or other serious disease manifestations. The purposes of this study were to examine if our patient population had a ... ...

    Abstract Purpose: Many patients with amyloidosis undergo carpal tunnel release (CTR) before amyloidosis diagnosis and before developing cardiac or other serious disease manifestations. The purposes of this study were to examine if our patient population had a similar prevalence of positive amyloidosis diagnoses to that in prior studies and to describe the results of implementing a screening program for amyloidosis.
    Methods: We retrospectively reviewed the biopsy results and subsequent interventions for all patients who underwent screening tenosynovial biopsy during CTR from March 2020 through December 2021. Amyloid screening was offered to patients who met the criteria for increased risk of disease using an appropriateness screening algorithm.
    Results: Seventy-five (48%) of 156 patients who underwent CTR met the eligibility criteria for amyloidosis testing. Of the 62 patients who agreed to undergo tenosynovial biopsy, 14 had amyloid-positive biopsy specimens (10 men and 4 women). All patients with positive tenosynovial biopsies had bilateral carpal tunnel syndrome and wild-type transthyretin amyloid subtype. One patient was diagnosed and started treatment for otherwise asymptomatic cardiac amyloidosis.
    Conclusions: The incidence of amyloid-positive tenosynovial biopsy results from CTR was 22.5% in patients using the criteria from an appropriateness screening algorithm, which was higher than previously reported. Implementation of a screening program for patients undergoing CTR requires a multidisciplinary approach and may result in early diagnosis and lifesaving interventions for patients with amyloidosis.
    Type of study/level of evidence: Differential diagnosis/symptom prevalence study, II.
    Language English
    Publishing date 2023-01-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 605716-0
    ISSN 1531-6564 ; 0363-5023
    ISSN (online) 1531-6564
    ISSN 0363-5023
    DOI 10.1016/j.jhsa.2022.09.005
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  7. Article ; Online: Serological evidence of exposure of healthy dogs to Leptospira in Sydney, New South Wales, Australia.

    Griebsch, C / Kirkwood, N / Ward, M P / Norris, J M

    Australian veterinary journal

    2024  Volume 102, Issue 4, Page(s) 215–221

    Abstract: In 2017, highly fatal canine leptospirosis emerged in Sydney, Australia. Based on results of microscopic agglutination testing (MAT), serovar Copenhageni appeared to be the most common causative serovar. Prior to this, no clinical cases had been reported ...

    Abstract In 2017, highly fatal canine leptospirosis emerged in Sydney, Australia. Based on results of microscopic agglutination testing (MAT), serovar Copenhageni appeared to be the most common causative serovar. Prior to this, no clinical cases had been reported since 1976. In a serosurvey of healthy dogs in Australian shelters in 2004, 2.4% of 431 New South Wales dogs had serological evidence of exposure to Copenhageni, the most prevalent serovar. The aim of this study was to estimate the current prevalence of Leptospira exposure and associated serovars in healthy Sydney dogs, previously unvaccinated against Leptospira. Serum samples from 411 healthy dogs in leptospirosis hotspots and neighbouring suburbs were collected before vaccination. MAT for 23 serovars was performed at the WHO Leptospirosis Reference Laboratory in Queensland, Australia. The overall seroprevalence was 4.1% (17/411) with low titres (1/50-1/200) detected. Eleven dogs were from known leptospirosis hotspots. Eight dogs were known to hunt rodents. One dog had been in contact with a leptospirosis positive dog 1 year prior. Serovar Topaz was the most prevalent serovar (n = 5) followed by serovars Australis (n = 4), Copenhageni (n = 4), Djasiman (n = 2), Cynopteri (n = 1), Javanica (n = 1), Medanensis (n = 1), and Pomona (n = 1). In conclusion, serological evidence of exposure of dogs in Sydney to Leptospira is low, but apparently has increased since 2004. Positive titres to serovars not previously reported to cause disease in dogs could be due to low virulence of those serovars or cross-reactivity with other serovars.
    MeSH term(s) Animals ; Dogs ; Leptospira ; New South Wales/epidemiology ; Seroepidemiologic Studies ; Australia ; Leptospirosis/epidemiology ; Leptospirosis/veterinary ; Leptospirosis/prevention & control ; Antibodies, Bacterial ; Dog Diseases
    Chemical Substances Antibodies, Bacterial
    Language English
    Publishing date 2024-01-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 41542-x
    ISSN 1751-0813 ; 0005-0423
    ISSN (online) 1751-0813
    ISSN 0005-0423
    DOI 10.1111/avj.13315
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  8. Article ; Online: To curb plastic pollution, industry and academia must unite.

    Ward, Collin P / Reddy, Christopher M / Edwards, Brian / Perri, Steven T

    Nature

    2024  Volume 625, Issue 7996, Page(s) 658–662

    MeSH term(s) Academia/organization & administration ; Environmental Pollution/legislation & jurisprudence ; Environmental Pollution/prevention & control ; Industry/organization & administration ; Plastics/analysis ; Plastics/supply & distribution
    Chemical Substances Plastics
    Language English
    Publishing date 2024-01-22
    Publishing country England
    Document type News
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/d41586-024-00155-z
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  9. Article ; Online: Trends in Permanent Work Disability Associated With Rheumatoid Arthritis in the United States, 1999-2015.

    Ward, Michael M

    Arthritis care & research

    2022  Volume 74, Issue 8, Page(s) 1321–1324

    Abstract: ... confidence interval 0.99-1.00]; P = 0.69). Risks of work disability were much higher among workers over age 50 years ...

    Abstract Objective: Advances in treatment over the past 20 years have resulted in improved control of rheumatoid arthritis (RA). The objective of our study was to investigate whether there has been a decrease in permanent work disability associated with RA in the US.
    Methods: Medicare data from 1999 to 2015 were used to identify beneficiaries age 20-59 years with RA who became eligible for Medicare coverage under Social Security Disability Insurance. Diagnosis of RA was based on physician claims in the first year of enrollment. Annual rates of enrollment were sex- and age-standardized to the 2000 US population.
    Results: The study included 97,787 beneficiaries with RA and Social Security Disability Insurance across all years. Medicare enrollment was 26.0 per million in 1999 and 26.0 per million in 2015. Rates increased following the Great Recession of 2008-2009 before returning to prerecession levels. There was no linear trend over time after adjusting for the annual national unemployment rate (relative risk 0.99 per year [95% confidence interval 0.99-1.00]; P = 0.69). Risks of work disability were much higher among workers over age 50 years.
    Conclusion: Based on Medicare enrollment by recipients of Social Security Disability Insurance, there was no decrease in permanent work disability among young and middle-age workers with RA in the US between 1999 and 2015.
    MeSH term(s) Adult ; Aged ; Arthritis, Rheumatoid/diagnosis ; Arthritis, Rheumatoid/epidemiology ; Disabled Persons ; Humans ; Medicare ; Middle Aged ; Unemployment ; United States/epidemiology ; Young Adult
    Language English
    Publishing date 2022-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural
    ZDB-ID 645059-3
    ISSN 2151-4658 ; 0893-7524 ; 2151-464X
    ISSN (online) 2151-4658
    ISSN 0893-7524 ; 2151-464X
    DOI 10.1002/acr.24575
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  10. Article ; Online: Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice.

    Kumar, Vidya P / Jaiswal, Shalini / Wuddie, Kefale / Ward, Jerrold M / Lawrence, Mark / Ghosh, Sanchita P

    Radiation research

    2024  

    Abstract: With the current volatile geopolitical climate, the threat of nuclear assault is high. Exposure to ionizing radiation from either nuclear incidents or radiological accidents often lead to major harmful consequences to human health. Depending on the ... ...

    Abstract With the current volatile geopolitical climate, the threat of nuclear assault is high. Exposure to ionizing radiation from either nuclear incidents or radiological accidents often lead to major harmful consequences to human health. Depending on the absorbed dose, the symptoms of the acute radiation syndrome and delayed effects of acute radiation exposure (DEARE) can appear within hours, weeks to months. The lung is a relatively radiosensitive organ with manifestation of radiation pneumonitis as an acute effect, followed by apparent fibrosis in weeks or even months. A recently developed, first-of-its-kind murine model for partial-body irradiation (PBI) injury, which can be used to test potential countermeasures against multi-organ damage such as gastrointestinal (GI) tract and lungs was used for irradiation, with 2.5% bone marrow spared (BM2.5-PBI) from radiation exposure. Long-term damage to lungs from radiation was evaluated using µ-CT scans, a pulmonary function test, histopathological parameters and molecular biomarkers. Pulmonary fibrosis was detected by ground glass opacity observed in µ-CT scans of male and female C57BL/6J mice 6-7 months after BM2.5-PBI. Lung mechanics assessments pertaining to peripheral airways suggested fibrotic lungs with stiffer parenchymal lung tissue and reduced inspiratory capacity in irradiated animals 6-7 months after BM2.5-PBI. Histopathological evaluation of the irradiated lungs revealed presence of focal and diffuse pleural, and parenchymal inflammatory and fibrotic lesions. Fibrosis was confirmed by elevated levels of collagen when compared to lungs of age-matched naïve mice. These findings were validated by findings of elevated levels of pro-fibrotic biomarkers and reduction in anti-inflammatory proteins. In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.
    Language English
    Publishing date 2024-02-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80322-4
    ISSN 1938-5404 ; 0033-7587
    ISSN (online) 1938-5404
    ISSN 0033-7587
    DOI 10.1667/RADE-23-00143.1
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