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Article ; Online: A hybrid electronic nose system for discrimination of pathogenic bacterial volatile compounds.

Seesaard, Thara / Thippakorn, Chadinee / Kerdcharoen, Teerakiat / Kladsomboon, Sumana

Analytical methods : advancing methods and applications

2020 Volume 12, Issue 47, Page(s) 5671–5683

Abstract: A hybrid electronic nose comprising an array of three organic-inorganic nanocomposite gas sensors [zinc tetra tert-butyl phthalocyanine (ZnTTBPc), zinc tetra-phenyl porphyrin (ZnTPP), and cobalt tetraphenyl-porphyrin (CoTPP)] coupled with three ... ...

Abstract	A hybrid electronic nose comprising an array of three organic-inorganic nanocomposite gas sensors [zinc tetra tert-butyl phthalocyanine (ZnTTBPc), zinc tetra-phenyl porphyrin (ZnTPP), and cobalt tetraphenyl-porphyrin (CoTPP)] coupled with three commercial metal-oxide semiconductor gas sensors (TGS 2444, TGS 2603 and TGS 2620) was developed to discriminate bacterial volatile compounds. Each type of gas sensor had its own strengths and weaknesses in terms of its capability to detect complex odors from the five different bacterial species tested. Bacterial samples were controlled at a fixed initial bacterial concentration by measuring the optical density at 600 nm of the culture suspensions. A comparative evaluation of the volatile compound fingerprints from five bacterial species grown in Luria-Bertani medium was conducted to identify the optimal incubation time for detection of volatile biomarkers to discriminate among bacteria. The results suggest that the hybrid electronic nose was indeed able to discriminate among the bacterial species and culture media, with a variance based on contributions of 92.4% from PC1 and 7.2% from PC2, at an incubation time of 6 hours. Furthermore, the results of hierarchical cluster analysis showed that bacterial odor data formed two major bacterial groups, with the maximum cluster distance close to 25. Intra-group similarity was demonstrated as the two bacterial species (E. cloacae and P. aeruginosa) from among the Gram-negative bacteria had a greater similarity with a cluster distance close to 4. Finally, the minimum distance between E. cloacae and S. Typhi was approximately 1, at an equal distance from E. coli and S. aureus.
MeSH term(s)	Bacteria ; Electronic Nose ; Escherichia coli ; Staphylococcus aureus ; Volatile Organic Compounds
Chemical Substances	Volatile Organic Compounds
Language	English
Publishing date	2020-11-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d0ay01255f
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A hybrid electronic nose system for discrimination of pathogenic bacterial volatile compounds

Seesaard, Thara / Thippakorn, Chadinee / Kerdcharoen, Teerakiat / Kladsomboon, Sumana

Analytical methods. 2020 Dec. 17, v. 12, no. 47

2020

Abstract: A hybrid electronic nose comprising an array of three organic–inorganic nanocomposite gas sensors [zinc tetra tert-butyl phthalocyanine (ZnTTBPc), zinc tetra-phenyl porphyrin (ZnTPP), and cobalt tetraphenyl-porphyrin (CoTPP)] coupled with three ... ...

Abstract	A hybrid electronic nose comprising an array of three organic–inorganic nanocomposite gas sensors [zinc tetra tert-butyl phthalocyanine (ZnTTBPc), zinc tetra-phenyl porphyrin (ZnTPP), and cobalt tetraphenyl-porphyrin (CoTPP)] coupled with three commercial metal-oxide semiconductor gas sensors (TGS 2444, TGS 2603 and TGS 2620) was developed to discriminate bacterial volatile compounds. Each type of gas sensor had its own strengths and weaknesses in terms of its capability to detect complex odors from the five different bacterial species tested. Bacterial samples were controlled at a fixed initial bacterial concentration by measuring the optical density at 600 nm of the culture suspensions. A comparative evaluation of the volatile compound fingerprints from five bacterial species grown in Luria–Bertani medium was conducted to identify the optimal incubation time for detection of volatile biomarkers to discriminate among bacteria. The results suggest that the hybrid electronic nose was indeed able to discriminate among the bacterial species and culture media, with a variance based on contributions of 92.4% from PC1 and 7.2% from PC2, at an incubation time of 6 hours. Furthermore, the results of hierarchical cluster analysis showed that bacterial odor data formed two major bacterial groups, with the maximum cluster distance close to 25. Intra-group similarity was demonstrated as the two bacterial species (E. cloacae and P. aeruginosa) from among the Gram-negative bacteria had a greater similarity with a cluster distance close to 4. Finally, the minimum distance between E. cloacae and S. Typhi was approximately 1, at an equal distance from E. coli and S. aureus.
Keywords	Escherichia coli ; absorbance ; biomarkers ; cluster analysis ; cobalt ; electronic nose ; nanocomposites ; odors ; porphyrins ; semiconductors ; variance ; volatile compounds ; zinc
Language	English
Dates of publication	2020-1217
Size	p. 5671-5683.
Publishing place	The Royal Society of Chemistry
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d0ay01255f
Database	NAL-Catalogue (AGRICOLA)

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Article: Proteomic and bioinformatic discovery of biomarkers for diabetic nephropathy.

Thippakorn, Chadinee / Schaduangrat, Nalini / Nantasenamat, Chanin

EXCLI journal

2018 Volume 17, Page(s) 312–330

Abstract: Diabetes is associated with numerous metabolic and vascular risk factors that contribute to a high rate of micro-vascular and macro-vascular disorders leading to mortality and morbidity from diabetic complications. In this case, the major cause of death ... ...

Abstract	Diabetes is associated with numerous metabolic and vascular risk factors that contribute to a high rate of micro-vascular and macro-vascular disorders leading to mortality and morbidity from diabetic complications. In this case, the major cause of death in overall diabetic patients results from diabetic nephropathy (DN) or renal failure. The risk factors and mechanisms that correspond to the development of DN are not fully understood and so far, no specific and sufficient diagnostic biomarkers are currently available other than micro- or macroalbuminuria. Therefore, this review describes current and novel protein biomarkers in the context of DN as well as probable proteins biomarkers associated with pathological processes for the early stage of DN via proteomics data together with bioinformatics. In addition, the mechanisms involved in early development of diabetic vascular disorders and complications resulting from glucose induced oxidative stress will also be explored.
Language	English
Publishing date	2018-03-26
Publishing country	Germany
Document type	Journal Article ; Review
ISSN	1611-2156
ISSN	1611-2156
DOI	10.17179/excli2018-1150
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Development of Organic-Inorganic Hybrid Optical Gas Sensors for the Non-Invasive Monitoring of Pathogenic Bacteria.

Kladsomboon, Sumana / Thippakorn, Chadinee / Seesaard, Thara

Sensors (Basel, Switzerland)

2018 Volume 18, Issue 10

Abstract: Hybrid optical gas sensors, based on different organic and inorganic materials, are proposed in this paper, with the aim of using them as optical artificial nose systems. Three types of organic and inorganic dyes, namely zinc-porphyrin, manganese- ... ...

Abstract	Hybrid optical gas sensors, based on different organic and inorganic materials, are proposed in this paper, with the aim of using them as optical artificial nose systems. Three types of organic and inorganic dyes, namely zinc-porphyrin, manganese-porphyrin, and zinc-phthalocyanine, were used as gas sensing materials to fabricate a thin-film coating on glass substrates. The performance of the gas sensor was enhanced by a thermal treatment process. The optical absorption spectra and morphological structure of the sensing films were confirmed by UV-Vis spectrophotometer and atomic force microscope, respectively. The optical gas sensors were tested with various volatile compounds, such as acetic acid, acetone, ammonia, ethanol, ethyl acetate, and formaldehyde, which are commonly found to be released during the growth of bacteria. These sensors were used to detect and discriminate between the bacterial odors of three pathogenic species (
MeSH term(s)	Bacteria/chemistry ; Bacteria/growth & development ; Bacteria/isolation & purification ; Bacteria/pathogenicity ; Electronic Nose ; Gases/analysis ; Gases/chemistry ; Odorants/analysis ; Porphyrins/chemistry ; Volatile Organic Compounds/analysis ; Volatile Organic Compounds/chemistry ; Volatilization
Chemical Substances	Gases ; Porphyrins ; Volatile Organic Compounds
Language	English
Publishing date	2018-09-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s18103189
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Oxidative responses and defense mechanism of hyperpigmented

Thippakorn, Chadinee / Isarankura-Na-Ayudhya, Chartchalerm / Pannengpetch, Supitcha / Isarankura-Na-Ayudhya, Patcharee / Schaduangrat, Nalini / Nantasenamat, Chanin / Prachayasittikul, Virapong

EXCLI journal

2018 Volume 17, Page(s) 544–562

Abstract: Pseudomonas ... ...

Abstract	Pseudomonas aeruginosa
Language	English
Publishing date	2018-06-13
Publishing country	Germany
Document type	Journal Article
ISSN	1611-2156
ISSN	1611-2156
DOI	10.17179/excli2018-1238
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Metal complexation by histidine-rich peptides confers protective roles against cadmium stress in

Isarankura-Na-Ayudhya, Patcharee / Thippakorn, Chadinee / Pannengpetch, Supitcha / Roytrakul, Sittiruk / Isarankura-Na-Ayudhya, Chartchalerm / Bunmee, Nipawan / Sawangnual, Suchitra / Prachayasittikul, Virapong

PeerJ

2018 Volume 6, Page(s) e5245

Abstract: The underlying mechanism and cellular responses of bacteria against toxic cadmium ions is still not fully understood. Herein, ...

Abstract	The underlying mechanism and cellular responses of bacteria against toxic cadmium ions is still not fully understood. Herein,
Language	English
Publishing date	2018-07-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.5245
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Roles of kininogen-1, basement membrane specific heparan sulfate proteoglycan core protein, and roundabout homolog 4 as potential urinary protein biomarkers in diabetic nephropathy.

Na Nakorn, Piyada / Pannengpetch, Supitcha / Isarankura-Na-Ayudhya, Patcharee / Thippakorn, Chadinee / Lawung, Ratana / Sathirapongsasuti, Nuankanya / Kitiyakara, Chagriya / Sritara, Piyamitr / Vathesatogkit, Prin / Isarankura-Na-Ayudhya, Chartchalerm

EXCLI journal

2020 Volume 19, Page(s) 872–891

Abstract: Diabetic nephropathy, a major complication of diabetes mellitus (DM), is increasing worldwide and the large majority of patients have type 2 DM. Microalbuminuria has been used as a diagnostic marker of diabetic nephropathy. But owing to its insufficient ... ...

Abstract	Diabetic nephropathy, a major complication of diabetes mellitus (DM), is increasing worldwide and the large majority of patients have type 2 DM. Microalbuminuria has been used as a diagnostic marker of diabetic nephropathy. But owing to its insufficient sensitivity and specificity, other biomarkers are being sought. In addition, the pathophysiology of diabetic nephropathy is not fully understood and declines in renal function occur even without microalbuminuria. In this study, we investigated urinary proteins from three study groups (controls, and type 2 diabetic subjects with or without microalbuminuria). Non-targeted label-free Nano-LC QTOF analysis was conducted to discover underlying mechanisms and protein networks, and targeted label-free Nano-LC QTOF with SWATH was performed to qualify discovered protein candidates. Twenty-eight proteins were identified as candidates and functionally analyzed via String DB, gene ontology and pathway analysis. Four predictive mechanisms were analyzed: i) response to stimulus, ii) platelet activation, signaling and aggregation, iii) ECM-receptor interaction, and iv) angiogenesis. These mechanisms can provoke kidney dysfunction in type 2 diabetic patients via endothelial cell damage and glomerulus structural alteration. Based on these analyses, three proteins (kininogen-1, basement membrane-specific heparan sulfate proteoglycan core protein, and roundabout homolog 4) were proposed for further study as potential biomarkers. Our findings provide insights that may improve methods for both prevention and diagnosis of diabetic nephropathy.
Language	English
Publishing date	2020-06-24
Publishing country	Germany
Document type	Journal Article
ISSN	1611-2156
ISSN	1611-2156
DOI	10.17179/excli2020-1396
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Metal complexation by histidine-rich peptides confers protective roles against cadmium stress in Escherichia coli as revealed by proteomics analysis

Patcharee Isarankura-Na-Ayudhya / Chadinee Thippakorn / Supitcha Pannengpetch / Sittiruk Roytrakul / Chartchalerm Isarankura-Na-Ayudhya / Nipawan Bunmee / Suchitra Sawangnual / Virapong Prachayasittikul

PeerJ, Vol 6, p e

2018 Volume 5245

Abstract: The underlying mechanism and cellular responses of bacteria against toxic cadmium ions is still not fully understood. Herein, Escherichia coli TG1 expressing hexahistidine-green fluorescent protein (His6GFP) and cells expressing polyhistidine-fused to ... ...

Abstract	The underlying mechanism and cellular responses of bacteria against toxic cadmium ions is still not fully understood. Herein, Escherichia coli TG1 expressing hexahistidine-green fluorescent protein (His6GFP) and cells expressing polyhistidine-fused to the outer membrane protein A (His-OmpA) were applied as models to investigate roles of cytoplasmic metal complexation and metal chelation at the surface membrane, respectively, upon exposure to cadmium stress. Two-dimensional gel electrophoresis (2-DE) and two-dimensional difference in gel electrophoresis (2D-DIGE) in conjunction with mass spectrometry-based protein identification had successfully revealed the low level expression of antioxidative enzymes and stress-responsive proteins such as manganese-superoxide dismutase (MnSOD; +1.65 fold), alkyl hydroperoxide reductase subunit C (AhpC; +1.03 fold) and DNA starvation/stationary phase protection protein (Dps; −1.02 fold) in cells expressing His6GFP in the presence of 0.2 mM cadmium ions. By contrarily, cadmium exposure led to the up-regulation of MnSOD of up to +7.20 and +3.08 fold in TG1-carrying pUC19 control plasmid and TG1 expressing native GFP, respectively, for defensive purposes against Cd-induced oxidative cell damage. Our findings strongly support the idea that complex formation between cadmium ions and His6GFP could prevent reactive oxygen species (ROS) caused by interaction between Cd2+ and electron transport chain. This coincided with the evidence that cells expressing His6GFP could maintain their growth pattern in a similar fashion as that of the control cells even in the presence of harmful cadmium. Interestingly, overexpression of either OmpA or His-OmpA in E. coli cells has also been proven to confer protection against cadmium toxicity as comparable to that observed in cells expressing His6GFP. Blockage of metal uptake as a consequence of anchored polyhistidine residues on surface membrane limited certain amount of cadmium ions in which some portion could pass through and exert their toxic ...
Keywords	Cadmium stress ; Polyhistidine ; Proteomics ; Metal complexation ; Outer membrane protein ; Medicine ; R ; Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2018-07-01T00:00:00Z
Publisher	PeerJ Inc.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Retinol-binding protein 4 and its potential roles in hypercholesterolemia revealed by proteomics.

Jugnam-Ang, Watcharapong / Pannengpetch, Supitcha / Isarankura-Na-Ayudhya, Patcharee / Thippakorn, Chadinee / Isarankura-Na-Ayudhya, Chartchalerm / Lawung, Ratana / Prachayasittiku, Virapong

EXCLI journal

2015 Volume 14, Page(s) 999–1013

Abstract: Effects of hypercholesterolemia on alterations of serum proteins have not been fully elucidated. Herein, using two-dimensional gel electrophoresis (2-DE) in conjunction with LC-MS searching has successfully been carried out to investigate the change of ... ...

Abstract	Effects of hypercholesterolemia on alterations of serum proteins have not been fully elucidated. Herein, using two-dimensional gel electrophoresis (2-DE) in conjunction with LC-MS searching has successfully been carried out to investigate the change of protein expression profiles as consequences of raised blood cholesterol at different levels (normal group: total cholesterol 200 mg/dL; borderline high group: total cholesterol 200-239 mg/dL; and high group: total cholesterol ≥ 240 mg/dL) (n = 45). Results revealed that down-regulation of retinol-binding protein 4 (RBP4) (-2.26 fold), transthyretin (-1.25 fold) and gelsolin (-1.47 fold) was observed in the high group. Meanwhile, the other proteins such as haptoglobin, complement factor B and CD5 antigen-like protein were up-regulated upto +3.24, +1.96 and +2.04 fold, respectively. Confirmation by Western blotting revealed a significant reduction of RBP4 (approximately 50 %) in individual samples derived from the high group. Presumptive conclusion can be drawn that down-regulation of RBP4 might be attributable to the inflammation of adipocytes caused by the release of proinflammatory cytokines (e.g. tumor necrosis factor α and interleukin-1β) from adipose tissues. Moreover, the decrease of transthyretin might also be taken into accounts since it is known that the transthyretin usually forms complex with RBP4 to prevent glomerular filtration and excretion through the kidney. The suppressing effect on RBP4 should be potentiated by the increase of complement factor B and CD5 antigen-like protein, which rendered the adipose tissues to overwhelm the liberation of RBP4 to blood circulation by metabolic and inflammatory processes. Such inflammation could further modulate the induction of cytokine release (e.g. IL-6 and IL-1β), resulting in the synthesis of acute phase protein, in particular, haptoglobin and C-reactive proteins from hepatocytes. However, the mechanism of gelsolin reduction remains unclear. Among these differentially expressed proteins, the RBP4 has been proposed as a major linkage between hypercholesterolemia, adipose tissues, liver and kidney, which is believed to be a potential biomarker for metabolic and cardiovascular disorders associated with dyslipidemia in the future.
Language	English
Publishing date	2015-08-28
Publishing country	Germany
Document type	Journal Article
ISSN	1611-2156
ISSN	1611-2156
DOI	10.17179/excli2015-478
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Proteomic alterations of Escherichia coli by paraquat.

Isarankura-Na-Ayudhya, Patcharee / Isarankura-Na-Ayudhya, Chartchalerm / Yainoy, Sakda / Thippakorn, Chadinee / Singhagamol, Watsarach / Polprachum, Wilaiwan / Roytrakul, Sittiruk / Prachayasittikul, Virapong

EXCLI journal

2010 Volume 9, Page(s) 108–118

Abstract: Paraquat (PQ; a widely used herbicide) exerts its harmful effect to human, mammals and microorganisms upon intracellular conversion to superoxide radical. Cellular responses against toxic paraquat remain not fully understood, especially on the adaptive ... ...

Abstract	Paraquat (PQ; a widely used herbicide) exerts its harmful effect to human, mammals and microorganisms upon intracellular conversion to superoxide radical. Cellular responses against toxic paraquat remain not fully understood, especially on the adaptive metabolic changes as a consequence of oxidative burden. In this study, alterations of metabolic processes of
Language	English
Publishing date	2010-09-28
Publishing country	Germany
Document type	Journal Article
ISSN	1611-2156
ISSN	1611-2156
Database	MEDical Literature Analysis and Retrieval System OnLINE

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