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Article ; Online: Identification of biomarkers for colorectal cancer through proteomics-based approaches.

Tjalsma, Harold

2010 Volume 7, Issue 6, Page(s) 879–895

Abstract: The early detection of colorectal cancer is one of the great challenges in the battle against this disease. However, owing to its heterogeneous character, single markers are not likely to provide sufficient diagnostic power to be used in colorectal ... ...

Abstract	The early detection of colorectal cancer is one of the great challenges in the battle against this disease. However, owing to its heterogeneous character, single markers are not likely to provide sufficient diagnostic power to be used in colorectal cancer population screens. This review provides an overview of recent studies aimed at the discovery of new diagnostic protein markers through proteomics-based approaches. It indicates that studies that start with the proteomic analysis of tumor tissue or tumor cell lines (near the source) have a high potential to yield novel and colorectal cancer-specific biomarkers. In the next step, the diagnostic accuracy of these candidate markers can be assessed by a targeted ELISA assay using serum from colorectal cancer patients and healthy controls. Instead, direct proteomic analysis of serum yields predominantly secondary markers composed of fragments of abundant serum proteins that may be associated with tumor-associated protease activity, and alternatively, immunoproteomic analysis of the serum antibody repertoire provides a valuable tool to identify the molecular imprint of colorectal cancer-associated antigens directly from patient serum samples. The latter approach also allows a relatively easy translation into targeted assays. Eventually, multimarker assays should be developed to reach a diagnostic accuracy that meets the stringent criteria for colorectal cancer screening at the population level.
MeSH term(s)	Animals ; Biomarkers, Tumor/biosynthesis ; Biomarkers, Tumor/blood ; Colorectal Neoplasms/chemistry ; Colorectal Neoplasms/diagnosis ; Feces/chemistry ; Humans ; Proteome/analysis ; Proteomics/methods
Chemical Substances	Biomarkers, Tumor ; Proteome
Language	English
Publishing date	2010-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2299100-1
ISSN	1744-8387 ; 1478-9450
ISSN (online)	1744-8387
ISSN	1478-9450
DOI	10.1586/epr.10.81
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Article: Feature-based reappraisal of the Bacillus subtilis exoproteome.

Tjalsma, Harold

Proteomics

2007 Volume 7, Issue 1, Page(s) 73–81

Abstract: Proteomics-based verification of computer-assisted predictions on bacterial protein export have indicated that problems occur with the distinction between (Sec-type) signal peptides that govern protein secretion, and lipoprotein signal peptides or amino- ... ...

Abstract	Proteomics-based verification of computer-assisted predictions on bacterial protein export have indicated that problems occur with the distinction between (Sec-type) signal peptides that govern protein secretion, and lipoprotein signal peptides or amino-terminal membrane anchors that cause protein retention in the membrane. Therefore, the main aim of this study was to investigate whether feature-based predictions by the SecretomeP (SecP) algorithm will aid the proteomics-based analysis of protein export in Bacillus subtilis. The SecP algorithm is trained to recognize features such as secondary structure and disordered regions, which are generally present in secreted proteins. The results showed that membrane-retained proteins receive, in general, high SecP scores, similar to the scores of secretory proteins. Importantly, the SecP algorithm aided in the re-evaluation of a class of previously identified proteins that remain attached to the membrane despite the presence of an apparent Sec-type signal peptide. These so-called 'Sec-attached' proteins receive on average a lower SecP score, and several of these proteins could be unmasked as transmembrane proteins by combined SecP and signal peptide analyses. Finally, the present study suggests that feature-based outlier analysis may provide leads towards the discovery of novel special-purpose pathways for bacterial protein export.
MeSH term(s)	Algorithms ; Bacillus subtilis/metabolism ; Computer Simulation ; Cytoplasm/metabolism ; Membrane Proteins/metabolism ; Protein Sorting Signals/physiology ; Protein Structure, Secondary ; Protein Transport ; Proteome/metabolism
Chemical Substances	Membrane Proteins ; Protein Sorting Signals ; Proteome
Language	English
Publishing date	2007-01
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2032093-0
ISSN	1615-9861 ; 1615-9853
ISSN (online)	1615-9861
ISSN	1615-9853
DOI	10.1002/pmic.200600520
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Zs.A 1868: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Streptococcus gallolyticus

Taddese, Rahwa / Roelofs, Rian / Draper, Derk / Wu, Xinqun / Wu, Shaoguang / Swinkels, Dorine W / Tjalsma, Harold / Boleij, Annemarie

Frontiers in cellular and infection microbiology

2021 Volume 11, Page(s) 740704

Abstract: Objective: The opportunistic pathogen : Design and results: Transcription profiling of : Conclusion: This study shows that gut bacteria have the potential to modulate the expression of biotransformation pathways in colonic epithelial cells in an ... ...

Abstract	Objective: The opportunistic pathogen Design and results: Transcription profiling of Conclusion: This study shows that gut bacteria have the potential to modulate the expression of biotransformation pathways in colonic epithelial cells in an AhR-dependent manner. This offers a novel theory on the contribution of intestinal bacteria to the etiology of CRC by modifying the capacity of intestinal epithelial or (pre-)cancerous cells to (de)toxify dietary components, which could alter intestinal susceptibility to DNA damaging events.
MeSH term(s)	Animals ; Biotransformation ; Colorectal Neoplasms ; Cytochrome P-450 CYP1A1/genetics ; Cytochrome P-450 CYP1A1/metabolism ; Epithelial Cells/metabolism ; Mice ; Mice, Inbred C57BL ; Receptors, Aryl Hydrocarbon/genetics ; Receptors, Aryl Hydrocarbon/metabolism ; Streptococcus gallolyticus/metabolism
Chemical Substances	Receptors, Aryl Hydrocarbon ; Cyp1a1 protein, mouse (EC 1.14.14.1) ; Cytochrome P-450 CYP1A1 (EC 1.14.14.1)
Language	English
Publishing date	2021-10-27
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2021.740704
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Article ; Online: The itinerary of Streptococcus gallolyticus infection in patients with colonic malignant disease.

Boleij, Annemarie / Tjalsma, Harold

The Lancet. Infectious diseases

2013 Volume 13, Issue 8, Page(s) 719–724

Abstract: Bacteria constitute about 90% of all cells in the human body. The densest and most complex bacterial community is in the large intestine. This population is quite stable in healthy intestines, but intestinal disease distorts the ecological balance and ... ...

Abstract	Bacteria constitute about 90% of all cells in the human body. The densest and most complex bacterial community is in the large intestine. This population is quite stable in healthy intestines, but intestinal disease distorts the ecological balance and induces dysbiosis. Results of studies have indicated that the epithelial and metabolic changes that occur with colorectal cancer provide a competitive advantage to a subset of intestinal bacteria. Strikingly, however, Streptococcus gallolyticus gallolyticus (previously known as Streptococcus bovis biotype I) is one of the very few opportunistic pathogens that has been clinically linked to colonic malignant diseases. In this Personal View we describe how S. gallolyticus gallolyticus exploits its unique range of virulence features to cause infections in patients with colorectal cancer. We postulate that distinct virulence factors on one hand enable this bacterium to establish a symptomatic infection in susceptible individuals, and on the other hand make its ability to do this dependent on pre-existing colonic abnormalities. We believe that our current reconstruction of this route of infection aids understanding of how S. gallolyticus gallolyticus infections can be best exploited for early detection of colorectal cancer.
MeSH term(s)	Adenoma/complications ; Adenoma/diagnosis ; Adenoma/microbiology ; Bacterial Translocation ; Carcinoma/complications ; Carcinoma/diagnosis ; Carcinoma/microbiology ; Colorectal Neoplasms/complications ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/microbiology ; Early Detection of Cancer ; Endocarditis/complications ; Endocarditis/microbiology ; Humans ; Streptococcal Infections/complications ; Streptococcal Infections/microbiology ; Streptococcus bovis/pathogenicity ; Streptococcus bovis/physiology ; Virulence Factors
Chemical Substances	Virulence Factors
Language	English
Publishing date	2013-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	2061641-7
ISSN	1474-4457 ; 1473-3099
ISSN (online)	1474-4457
ISSN	1473-3099
DOI	10.1016/S1473-3099(13)70107-5
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Article ; Online: Gut bacteria in health and disease: a survey on the interface between intestinal microbiology and colorectal cancer.

Boleij, Annemarie / Tjalsma, Harold

Biological reviews of the Cambridge Philosophical Society

2012 Volume 87, Issue 3, Page(s) 701–730

Abstract: A healthy human body contains at least tenfold more bacterial cells than human cells and the most abundant and diverse microbial community resides in the intestinal tract. Intestinal health is not only maintained by the human intestine itself and by ... ...

Abstract	A healthy human body contains at least tenfold more bacterial cells than human cells and the most abundant and diverse microbial community resides in the intestinal tract. Intestinal health is not only maintained by the human intestine itself and by dietary factors, but is also largely supported by this resident microbial community. Conversely, however, a large body of evidence supports a relationship between bacteria, bacterial activities and human colorectal cancer. Symbiosis in this multifaceted organ is thus crucial to maintain a healthy balance within the host-diet-microbiota triangle and accordingly, changes in any of these three factors may drive a healthy situation into a state of disease. In this review, the factors that sustain health or drive this complex intestinal system into dysbiosis are discussed. Emphasis is on the role of the intestinal microbiota and related mechanisms that can drive the initiation and progression of sporadic colorectal cancer (CRC). These mechanisms comprise the induction of pro-inflammatory and pro-carcinogenic pathways in epithelial cells as well as the production of (geno)toxins and the conversion of pro-carcinogenic dietary factors into carcinogens. A thorough understanding of these processes will provide leads for future research and may ultimately aid in development of new strategies for CRC diagnosis and prevention.
MeSH term(s)	Colorectal Neoplasms/microbiology ; Diet ; Gastrointestinal Diseases/microbiology ; Gastrointestinal Tract/cytology ; Gastrointestinal Tract/immunology ; Gastrointestinal Tract/microbiology ; Humans ; Mucus
Language	English
Publishing date	2012-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1423558-4
ISSN	1469-185X ; 0006-3231 ; 1464-7931
ISSN (online)	1469-185X
ISSN	0006-3231 ; 1464-7931
DOI	10.1111/j.1469-185X.2012.00218.x
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Article ; Online: Nutritional iron turned inside out: intestinal stress from a gut microbial perspective.

Kortman, Guus A M / Raffatellu, Manuela / Swinkels, Dorine W / Tjalsma, Harold

FEMS microbiology reviews

2014 Volume 38, Issue 6, Page(s) 1202–1234

Abstract: Iron is abundantly present on earth, essential for most microorganisms and crucial for human health. Human iron deficiency that is nevertheless highly prevalent in developing regions of the world can be effectively treated by oral iron administration. ... ...

Abstract	Iron is abundantly present on earth, essential for most microorganisms and crucial for human health. Human iron deficiency that is nevertheless highly prevalent in developing regions of the world can be effectively treated by oral iron administration. Accumulating evidence indicates that excess of unabsorbed iron that enters the colonic lumen causes unwanted side effects at the intestinal host-microbiota interface. The chemical properties of iron, the luminal environment and host iron withdrawal mechanisms, especially during inflammation, can turn the intestine in a rather stressful milieu. Certain pathogenic enteric bacteria can, however, deal with this stress at the expense of other members of the gut microbiota, while their virulence also seems to be stimulated in an iron-rich intestinal environment. This review covers the multifaceted aspects of nutritional iron stress with respect to growth, composition, metabolism and pathogenicity of the gut microbiota in relation to human health. We aim to present an unpreceded view on the dynamic effects and impact of oral iron administration on intestinal host-microbiota interactions to provide leads for future research and other applications.
MeSH term(s)	Bacteria/drug effects ; Bacteria/immunology ; Biodiversity ; Host-Pathogen Interactions/drug effects ; Humans ; Intestines/immunology ; Intestines/microbiology ; Iron/chemistry ; Iron/metabolism ; Iron, Dietary/metabolism ; Iron, Dietary/pharmacology ; Oxidation-Reduction ; Stress, Physiological/drug effects
Chemical Substances	Iron, Dietary ; Iron (E1UOL152H7)
Language	English
Publishing date	2014-11
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	283740-7
ISSN	1574-6976 ; 0168-6445
ISSN (online)	1574-6976
ISSN	0168-6445
DOI	10.1111/1574-6976.12086
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Book ; Thesis: Signal peptidases of Bacillus subtilis

Tjalsma, Harold

a functional analysis

1999

Author's details	door Harold Tjalsma
Language	English
Size	196 S, Ill., graph. Darst
Publisher	Rijksuniv
Publishing place	Groningen
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ.,Diss.--Groningen, 1999
Accompanying material	stellingen
ISBN	9036710863 ; 9789036710862
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Association between Streptococcus bovis and colon cancer.

Boleij, Annemarie / Schaeps, Renée M J / Tjalsma, Harold

Journal of clinical microbiology

2009 Volume 47, Issue 2, Page(s) 516

MeSH term(s)	Colonic Neoplasms/microbiology ; Humans ; Streptococcal Infections/complications ; Streptococcal Infections/epidemiology ; Streptococcus bovis/isolation & purification
Language	English
Publishing date	2009-02-03
Publishing country	United States
Document type	Comment ; Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.01755-08
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Article ; Online: A bacterial driver-passenger model for colorectal cancer: beyond the usual suspects.

Tjalsma, Harold / Boleij, Annemarie / Marchesi, Julian R / Dutilh, Bas E

Nature reviews. Microbiology

2012 Volume 10, Issue 8, Page(s) 575–582

Abstract: Cancer has long been considered a genetic disease. However, accumulating evidence supports the involvement of infectious agents in the development of cancer, especially in those organs that are continuously exposed to microorganisms, such as the large ... ...

Abstract	Cancer has long been considered a genetic disease. However, accumulating evidence supports the involvement of infectious agents in the development of cancer, especially in those organs that are continuously exposed to microorganisms, such as the large intestine. Recent next-generation sequencing studies of the intestinal microbiota now offer an unprecedented view of the aetiology of sporadic colorectal cancer and have revealed that the microbiota associated with colorectal cancer contains bacterial species that differ in their temporal associations with developing tumours. Here, we propose a bacterial driver-passenger model for microbial involvement in the development of colorectal cancer and suggest that this model be incorporated into the genetic paradigm of cancer progression.
MeSH term(s)	Bacteria/classification ; Bacteria/genetics ; Bacteria/pathogenicity ; Bacterial Infections/complications ; Bacterial Infections/microbiology ; Biota ; Colorectal Neoplasms/microbiology ; Colorectal Neoplasms/pathology ; Humans ; Models, Biological
Language	English
Publishing date	2012-06-25
Publishing country	England
Document type	Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2139054-X
ISSN	1740-1534 ; 1740-1526
ISSN (online)	1740-1534
ISSN	1740-1526
DOI	10.1038/nrmicro2819
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Article ; Online: Iron availability increases the pathogenic potential of Salmonella typhimurium and other enteric pathogens at the intestinal epithelial interface.

Kortman, Guus A M / Boleij, Annemarie / Swinkels, Dorine W / Tjalsma, Harold

PloS one

2012 Volume 7, Issue 1, Page(s) e29968

Abstract: Recent trials have questioned the safety of untargeted oral iron supplementation in developing regions. Excess of luminal iron could select for enteric pathogens at the expense of beneficial commensals in the human gut microflora, thereby increasing the ... ...

Abstract	Recent trials have questioned the safety of untargeted oral iron supplementation in developing regions. Excess of luminal iron could select for enteric pathogens at the expense of beneficial commensals in the human gut microflora, thereby increasing the incidence of infectious diseases. The objective of the current study was to determine the effect of high iron availability on virulence traits of prevalent enteric pathogens at the host-microbe interface. A panel of enteric bacteria was cultured under iron-limiting conditions and in the presence of increasing concentrations of ferric citrate to assess the effect on bacterial growth, epithelial adhesion, invasion, translocation and epithelial damage in vitro. Translocation and epithelial integrity experiments were performed using a transwell system in which Caco-2 cells were allowed to differentiate to a tight epithelial monolayer mimicking the intestinal epithelial barrier. Growth of Salmonella typhimurium and other enteric pathogens was increased in response to iron. Adhesion of S. typhimurium to epithelial cells markedly increased when these bacteria were pre-incubated with increasing iron concentration (P = 0.0001), whereas this was not the case for the non-pathogenic Lactobacillus plantarum (P = 0.42). Cellular invasion and epithelial translocation of S. typhimurium followed the trend of increased adhesion. Epithelial damage was increased upon incubation with S. typhimurium or Citrobacter freundii that were pre-incubated under iron-rich conditions. In conclusion, our data fit with the consensus that oral iron supplementation is not without risk as iron could, in addition to inducing pathogenic overgrowth, also increase the virulence of prevalent enteric pathogens.
MeSH term(s)	Bacterial Adhesion/drug effects ; Bacterial Translocation/drug effects ; Biological Availability ; Caco-2 Cells ; Citrobacter freundii/growth & development ; Citrobacter freundii/physiology ; Dietary Supplements ; Dose-Response Relationship, Drug ; Enterococcus faecalis/growth & development ; Enterococcus faecalis/physiology ; Epithelium/metabolism ; Epithelium/microbiology ; Escherichia coli/growth & development ; Escherichia coli/physiology ; Ferric Compounds/pharmacology ; Host-Pathogen Interactions ; Humans ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/microbiology ; Intestines/microbiology ; Iron/metabolism ; Iron, Dietary/administration & dosage ; Iron, Dietary/metabolism ; Iron, Dietary/pharmacokinetics ; Lactobacillus plantarum/growth & development ; Lactobacillus plantarum/physiology ; Salmonella typhimurium/growth & development ; Salmonella typhimurium/physiology
Chemical Substances	Ferric Compounds ; Iron, Dietary ; ferric citrate (63G354M39Z) ; Iron (E1UOL152H7)
Language	English
Publishing date	2012-01-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0029968
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