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Book ; Thesis: Etablierung eines neuen Zellkulturmodells der Hepatitis-B-Virus, Hepatitis-C-Virus-Koinfektion

Präg, Chiara

immunologische Mechanismen der viralen Interaktion

2015

Author's details	vorgelegt 2015 von Chiara Präg
Language	German
Size	87 Seiten, Illustrationen, 21 cm
Publishing place	Freiburg im Breisgau
Publishing country	Germany
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Dissertation, Albert-Ludwigs-Universität Freiburg im Breisgau, 2015
HBZ-ID	HT018890626
Database	Catalogue ZB MED Medicine, Health

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Article ; Online: Does Children's Education Improve Parental Health and Longevity? Causal Evidence from Great Britain.

Potente, Cecilia / Präg, Patrick / Monden, Christiaan W S

Journal of health and social behavior

2023 Volume 64, Issue 1, Page(s) 21–38

Abstract: Parents with better-educated children are healthier and live longer, but whether there is a causal effect of children's education on their parents' health and longevity is unclear. First, we demonstrate an association between adults' offspring education ... ...

Abstract	Parents with better-educated children are healthier and live longer, but whether there is a causal effect of children's education on their parents' health and longevity is unclear. First, we demonstrate an association between adults' offspring education and parental mortality in the 1958 British birth cohort study, which remains substantial-about two additional years of life-even when comparing parents with similar socioeconomic status. Second, we use the 1972 educational reform in England and Wales, which increased the minimum school leaving age from 15 to 16 years, to identify the presence of a causal effect of children's education on parental health and longevity using census-linked data from the Office for National Statistics Longitudinal Study. Results reveal that children's education has no causal effects on a wide range of parental mortality and health outcomes. We interpret these findings discussing the role of universal health care and education for socioeconomic inequality in Great Britain.
MeSH term(s)	Adult ; Humans ; Child ; Adolescent ; Longevity ; Longitudinal Studies ; Cohort Studies ; United Kingdom ; Educational Status ; Parents
Language	English
Publishing date	2023-01-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218206-3
ISSN	2150-6000 ; 0022-1465
ISSN (online)	2150-6000
ISSN	0022-1465
DOI	10.1177/00221465221143089
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Article ; Online: Substitutional analysis of the C-terminal domain of AbrB revealed its essential role in DNA-binding activity.

Neubauer, Svetlana / Dolgova, Olga / Präg, Gregory / Borriss, Rainer / Makarewicz, Oliwia

PloS one

2014 Volume 9, Issue 5, Page(s) e97254

Abstract: ... the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA ...

Abstract	The global transition state regulator AbrB controls more than 100 genes of the Bacillus relatives and is known to interact with varying DNA-sequences. The DNA-binding domain of the AbrB-like proteins was proposed to be located exclusively within the amino-terminal ends. However, the recognition of DNA, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. Here we present a substitutional analysis to examine the role of the carboxy-terminal domain of AbrB from Bacillus subtilis and Bacillus amyloliquefaciens. Our results demonstrate that the carboxy-terminal domains of AbrB affect the DNA-binding properties of the tetrameric AbrB. Most likely, the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC.
MeSH term(s)	Amino Acid Sequence ; Bacillus/genetics ; Bacillus/metabolism ; Bacillus subtilis/genetics ; Bacillus subtilis/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; DNA/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Oligonucleotides/genetics ; Peptide Synthases/metabolism ; Promoter Regions, Genetic ; Protein Binding ; Protein Structure, Tertiary ; Sequence Homology, Amino Acid ; Transcription Factors/genetics ; Transcription Factors/metabolism
Chemical Substances	AbrB protein, Bacillus subtilis ; Bacterial Proteins ; DNA-Binding Proteins ; Oligonucleotides ; Transcription Factors ; DNA (9007-49-2) ; Peptide Synthases (EC 6.3.2.-) ; tyrocidine synthetase (EC 6.3.2.-)
Language	English
Publishing date	2014-05-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0097254
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Article: Regulation of post-translational modifications of muskelin by protein kinase C.

Prag, Soren / De Arcangelis, Adèle / Georges-Labouesse, Elisabeth / Adams, Josephine C

The international journal of biochemistry & cell biology

2007 Volume 39, Issue 2, Page(s) 366–378

Abstract: ... for phosphorylation by protein kinase C (PKC), are conserved between vertebrate and Drosophila muskelins ...

Abstract	Muskelin is a member of the kelch-repeat superfamily of proteins, identified as an intracellular protein involved in cell spreading responses to thombospondin-1. Muskelin is expressed by many adult tissues and has an evolutionarily conserved, multidomain architecture consisting of an amino-terminal discoidin-like domain, a central alpha-helical region and six kelch-repeats that are predicted to form a beta-propeller structure. We previous demonstrated that muskelin molecules undergo head-to-tail association, however the physiological, post-translational regulation of muskelin is not well understood. Here, we have examined the expression of muskelin during mouse embryonic development and report widespread expression that includes muscle tissues, multiple epithelia and the brain. In cultured skeletal myoblasts and vascular smooth muscle cells, muskelin exists as a complex set of isoelectric variants. Five potential sites for phosphorylation by protein kinase C (PKC), are conserved between vertebrate and Drosophila muskelins, therefore we examined the hypothesis that muskelin is regulated post-translationally by PKC activity. We demonstrate that PKC activation or inhibition regulates the profile of endogenous muskelin isoelectric variants and that muskelin is a substrate for PKCalphain vitro. Wild-type GFP-muskelin and a panel of alanine point mutations were used to test the sensitivity of self-association to PKC activation. Mutation of two of the sites, S324 and T515, partially inhibited the ability of muskelin to self-associate in cells and inhibited responsiveness to activated PKC. Interestingly, both sites are predicted to lie in surface-exposed loops on the same side of the beta-propeller, implicating a common binding interface.
MeSH term(s)	Animals ; COS Cells ; Cell Adhesion Molecules/genetics ; Cell Adhesion Molecules/metabolism ; Cercopithecus aethiops ; Embryo, Mammalian ; Gene Expression Regulation, Developmental ; Green Fluorescent Proteins/genetics ; Humans ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Isoelectric Point ; Mice ; Models, Molecular ; Phosphorylation ; Protein Conformation ; Protein Kinase C/genetics ; Protein Kinase C/metabolism ; Protein Processing, Post-Translational ; Protein Structure, Secondary ; Rats ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
Chemical Substances	Cell Adhesion Molecules ; Intracellular Signaling Peptides and Proteins ; Mkln1 protein, mouse ; Recombinant Proteins ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9) ; Protein Kinase C (EC 2.7.11.13)
Language	English
Publishing date	2007
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1228429-4
ISSN	1878-5875 ; 1357-2725
ISSN (online)	1878-5875
ISSN	1357-2725
DOI	10.1016/j.biocel.2006.09.003
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Article ; Online: Subjective socioeconomic status and self-rated health in the English Longitudinal Study of Aging: A fixed-effects analysis.

Coustaury, Camille / Jeannot, Elias / Moreau, Adele / Nietge, Clotilde / Maharani, Asri / Richards, Lindsay / Präg, Patrick

Social science & medicine (1982)

2023 Volume 336, Page(s) 116235

Abstract: Higher subjective socio-economic status (SES) goes along with better self-rated health: This finding is well-established in the literature, yet the majority of studies it is based on only rely on cross-sectional analyses and only account for few ... ...

Abstract	Higher subjective socio-economic status (SES) goes along with better self-rated health: This finding is well-established in the literature, yet the majority of studies it is based on only rely on cross-sectional analyses and only account for few potential confounders of the association. Particularly wealth, which is increasingly thought of as an important dimension of accumulated advantage, is only rarely examined as a confounder. Using eight waves of panel data from the English Longitudinal Study of Aging (ELSA, 2002-19), we investigate the association between subjective SES and self-rated health. We use random effects models that account for theoretically important time-constant (such as education and social class) and time-varying confounders (such as income and wealth) as well as fixed-effects models, that in addition control for all time-constant confounders, whether observed or unobserved. The fully adjusted fixed-effects model reveals a statistically significant association between subjective SES and self-rated health. Yet, a one-point increase on the subjective SES ladder goes along with a two per cent of a standard deviation increase in self-rated health, only around a quarter of the size of the random-effects estimate. The role of wealth for the subjective SES-self-rated health association is negligible in the fixed-effects specifications. Smoking, drinking, and physical activity do not appear to mediate the association. A substantial part, though not all, of the observed association between subjective SES and self-rated health is due to unobserved confounding rather than a causal effect. Reducing health inequalities based on objective SES is likely more effective than based on subjective SES.
MeSH term(s)	Humans ; Longitudinal Studies ; Cross-Sectional Studies ; Social Class ; Aging ; Income ; Health Status ; Socioeconomic Factors
Language	English
Publishing date	2023-09-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	4766-1
ISSN	1873-5347 ; 0037-7856 ; 0277-9536
ISSN (online)	1873-5347
ISSN	0037-7856 ; 0277-9536
DOI	10.1016/j.socscimed.2023.116235
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Article ; Online: Believing in conspiracy theories in Spain during the COVID-19 pandemic: Drivers and public health implications.

Nefes, Türkay Salim / Präg, Patrick / Romero-Reche, Alejandro / Pereira-Puga, Manuel

Social science & medicine (1982)

2023 Volume 336, Page(s) 116263

Abstract: ... education, (c) related to instrumental rationality considerations, and (d) only weakly related to value ...

Abstract	Conspiracy theories jeopardize public health by disseminating misinformation and undermining authoritative health guidelines. This study explores social factors associated with the belief in conspiracy theories in Spain during the COVID-19 pandemic. Drawing upon the theoretical framework of Max Weber, it posits that beliefs in conspiracy theories are linked to both instrumental rationality considerations, such as trust in health authorities, science, and pharmaceutical companies, as well as value-rationality based factors, such as ideological orientation. The study analyzes recent, nationally representative survey data and is the first to examine the social predictors of belief in conspiracy theories in Spain during the pandemic. The findings highlight that conspiracy theory beliefs are (a) associated with considerably worse vaccination behaviors, (b) not or only very weakly associated with standard demographics such as age, sex, or education, (c) related to instrumental rationality considerations, and (d) only weakly related to value-rationality indicators such as ideological and religious affiliations. In conclusion, the study underscores the significance of public health policies that specifically address conspiracy theory convictions, and to that end, advocates for the application of a Weberian sociological perspective to better understand the diverse rationalities underlying these beliefs, particularly in the absence of discernible demographic predictors.
MeSH term(s)	Humans ; COVID-19/epidemiology ; Spain/epidemiology ; Pandemics ; Public Health ; Educational Status
Language	English
Publishing date	2023-09-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	4766-1
ISSN	1873-5347 ; 0037-7856 ; 0277-9536
ISSN (online)	1873-5347
ISSN	0037-7856 ; 0277-9536
DOI	10.1016/j.socscimed.2023.116263
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Article ; Online: Substitutional analysis of the C-terminal domain of AbrB revealed its essential role in DNA-binding activity.

Svetlana Neubauer / Olga Dolgova / Gregory Präg / Rainer Borriss / Oliwia Makarewicz

PLoS ONE, Vol 9, Iss 5, p e

2014 Volume 97254

Abstract: ... the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA ...

Abstract	The global transition state regulator AbrB controls more than 100 genes of the Bacillus relatives and is known to interact with varying DNA-sequences. The DNA-binding domain of the AbrB-like proteins was proposed to be located exclusively within the amino-terminal ends. However, the recognition of DNA, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. Here we present a substitutional analysis to examine the role of the carboxy-terminal domain of AbrB from Bacillus subtilis and Bacillus amyloliquefaciens. Our results demonstrate that the carboxy-terminal domains of AbrB affect the DNA-binding properties of the tetrameric AbrB. Most likely, the C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Intergenerational social mobility and allostatic load in Great Britain.

Präg, Patrick / Richards, Lindsay

Journal of epidemiology and community health

2018 Volume 73, Issue 2, Page(s) 100–105

Abstract: ... triglycerides, (4) glycated haemoglobin, (5) C-reactive protein, (6) fibrinogen, (7) systolic blood pressure, (8 ...

Abstract	Background: Intergenerational social mobility is hypothesised to be a stressful process that has a negative effect on health. By examining the relationship between own socioeconomic position, parental socioeconomic position and allostatic load (AL) in a representative sample of the British population, we test this hypothesis. Methods: Our study uses cross-sectional data from 9851 adult participants of waves 2 and 3 of Understanding Society. The relationship between parental occupational class at age 14 years, respondents' social class at the time of the interview and AL is explored by means of diagonal reference models, which allow us to disentangle the effects of parental social class, own social class and the mobility process. The AL score comprises the following biomarkers: (1) total cholesterol, (2) high-density lipoprotein cholesterol, (3) triglycerides, (4) glycated haemoglobin, (5) C-reactive protein, (6) fibrinogen, (7) systolic blood pressure, (8) diastolic blood pressure, (9) resting heart rate, (10) body mass index and (11) waist circumference. Results: AL is particularly high among the stable working class and low among the stable upper class. On average, current class and origin class exert about equal weight on current AL. However, social mobility-regardless of whether upwards or downwards-is not detrimental for AL. Furthermore, we find evidence that class of origin may be less important among those outside the labour market for reasons other than retirement. Conclusion: Both own social class and parental social class influence AL to a similar extent. However, we find no evidence that mobility trajectories exert any effects, good or bad, on AL.
MeSH term(s)	Adolescent ; Adult ; Allostasis/physiology ; C-Reactive Protein/analysis ; Cross-Sectional Studies ; Female ; Glycated Hemoglobin A/analysis ; Humans ; Male ; Middle Aged ; Social Class ; Social Mobility ; United Kingdom
Chemical Substances	Glycated Hemoglobin A ; C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2018-11-01
Publishing country	England
Document type	Journal Article
ZDB-ID	391868-3
ISSN	1470-2738 ; 0142-467X ; 0141-7681 ; 0143-005X
ISSN (online)	1470-2738
ISSN	0142-467X ; 0141-7681 ; 0143-005X
DOI	10.1136/jech-2017-210171
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Article ; Online: Cultural determinants influence assisted reproduction usage in Europe more than economic and demographic factors.

Präg, Patrick / Mills, Melinda C

Human reproduction (Oxford, England)

2017 Volume 32, Issue 11, Page(s) 2305–2314

Abstract: ... Strategic Support Fund (all to M.C.M.). The authors have no conflicts of interest to declare.: Trial ...

Abstract	Study question: To what extent do financial, demographic and cultural determinants explain the vast cross-national differences in ART treatments in Europe? Summary answer: The normative cultural acceptance of ART is a major driver of ART treatments in Europe, above and beyond differences in country wealth, demographic aspects and religious composition. What is known already: There are vast differences in the number of ART treatments across European countries, which are to some extent related to country affluence, regulation, and insurance coverage and costs. The role and impact of cultural and normative factors has not been explored in a larger cross-national comparison. Study design, size, duration: A descriptive and comparative cross-national analysis of ART treatment prevalence in over 30 European countries in 2010, with the outcome defined as the total number of ART cycles per million women of reproductive age (15-44 years). Data is drawn from multiple sources (ICMART, US Census Bureau Library, World Bank, Barro-Lee Educational Attainment Dataset, IFFS Surveillance reports, European Values Study and World Religion Database). Participants/materials, setting, methods: Our sample includes data from 35 European countries, where we describe the associations between demographic and cultural factors and the prevalence of ART treatments. Bivariate correlation and ordinary least squares multiple regression analysis serves to establish the relationships between predictor variables and the number of ART treatments per million women aged 15-44 years in a country. Main results and the role of chance: A one-percent increase in national GDP is associated with 382 (95% CI: 177-587) additional ART procedures per million women of reproductive age, yet this effect is reduced to 99 (-92 to 290) treatments once cultural values and demographic factors are accounted for. In our fully adjusted model, normative cultural values measuring the acceptability of ART are the strongest predictor of ART usage, with a one-point increase of average approval in a country associated with 276 (167-385) additional ART treatments per million women of reproductive age. Limitations, reasons for caution: Findings are based on a cross-sectional, cross-national analysis, making formal tests of causality impossible and prohibiting inferences to the individual level. Wider implications of the findings: Results indicate that reproductive health policy should openly acknowledge the importance of cultural norms in informally shaping and regulating the wider availability of ART treatment. Study funding/competing interest(s): Funding for this project was provided by the European Union's Seventh Framework Program (FP7 2007-2013) (No. 320116 Families and Societies), European Research Council for the SOCIOGENOME Consolidator Grant (ERC-2013-CoG-615603) and the Wellcome Trust Institutional Strategic Support Fund (all to M.C.M.). The authors have no conflicts of interest to declare. Trial registration number: N/A.
MeSH term(s)	Adolescent ; Adult ; Age Factors ; Cross-Sectional Studies ; Culture ; Europe ; Female ; Humans ; Pregnancy ; Pregnancy Rate ; Registries ; Reproductive Techniques, Assisted/economics ; Reproductive Techniques, Assisted/statistics & numerical data ; Socioeconomic Factors ; Young Adult
Language	English
Publishing date	2017-12-21
Publishing country	England
Document type	Journal Article
ZDB-ID	632776-x
ISSN	1460-2350 ; 0268-1161 ; 1477-741X
ISSN (online)	1460-2350
ISSN	0268-1161 ; 1477-741X
DOI	10.1093/humrep/dex298
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Article ; Online: Intergenerational Social Mobility and Allostatic Load in Midlife and Older Ages: A Diagonal Reference Modeling Approach.

McLoughlin, Sinéad / Präg, Patrick / Bartley, Mel / Kenny, Rose Anne / McCrory, Cathal

The journals of gerontology. Series B, Psychological sciences and social sciences

2022 Volume 78, Issue 1, Page(s) 154–166

Abstract: Objectives: This study aims to understand the association of life-course intergenerational social mobility with allostatic load (AL) burden in midlife and older ages in Ireland.: Methods: The study involved biological data for 3,987 older adults ... ...

Abstract	Objectives: This study aims to understand the association of life-course intergenerational social mobility with allostatic load (AL) burden in midlife and older ages in Ireland. Methods: The study involved biological data for 3,987 older adults participating in The Irish Longitudinal Study on Ageing (TILDA). Intergenerational social mobility was characterized using the cross-classification of origin socioeconomic position (SEP; i.e., father's occupation) and destination SEP (i.e., own occupation). AL was operationalized using 12 biomarkers tapping cardiovascular, metabolic, renal, and immune system dysregulation. Diagonal reference modeling (DRM) and ordinary least square regression techniques were applied to explore the effect of social mobility on AL burden. Results: A total of 55.5% experienced intergenerational mobility: 37.5% were upwardly mobile, 18.0% were downwardly mobile. A social gradient in AL was observed among the socially non-mobile. Destination SEP (b = 0.74, 95% CI = 0.57, 0.92) predominated in influence over origin, although both life stages exerted significant influence on later-life AL. Social mobility in either direction was not associated with AL burden. Mobility coefficients were substantially small across a large variety of model specifications. Discussion: Findings provide evidence for an accumulation model of social inequalities in which disparities in health are diluted rather than increased by social mobility (i.e., gradient constraint), with the socially mobile having an AL score that is intermediate between their origin class and destination class. This implies that the effects of origin SEP on health are not immutable, but are instead responsive to changing socioeconomic circumstances across the life course.
MeSH term(s)	Humans ; Aged ; Middle Aged ; Social Mobility ; Longitudinal Studies ; Allostasis/physiology ; Socioeconomic Factors ; Aging/physiology ; Social Class
Language	English
Publishing date	2022-08-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1223664-0
ISSN	1758-5368 ; 1079-5014
ISSN (online)	1758-5368
ISSN	1079-5014
DOI	10.1093/geronb/gbac122
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