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Article ; Online: Clinical, dosimetric, and reporting considerations for Y-90 glass microspheres in hepatocellular carcinoma: updated 2022 recommendations from an international multidisciplinary working group.

Salem, Riad / Padia, Siddharth A / Lam, Marnix / Chiesa, Carlo / Haste, Paul / Sangro, Bruno / Toskich, Beau / Fowers, Kirk / Herman, Joseph M / Kappadath, S Cheenu / Leung, Thomas / Sze, Daniel Y / Kim, Edward / Garin, Etienne

European journal of nuclear medicine and molecular imaging

2022 Volume 50, Issue 2, Page(s) 328–343

Abstract: ... in the treatment of HCC with yttrium-90 (Y-90) glass microsphere-based transarterial radioembolization (TARE ... in patients with HCC to support more individualized efficacious treatment with Y-90 glass microspheres ... recommendations are provided to guide clinical and dosimetric approaches for the use of Y-90 glass microsphere ...

Abstract	Purpose: In light of recently published clinical reports and trials, the TheraSphere Global Dosimetry Steering Committee (DSC) reconvened to review new data and to update previously published clinical and dosimetric recommendations for the treatment of hepatocellular carcinoma (HCC). Methods: The TheraSphere Global DSC is comprised of health care providers across multiple disciplines involved in the treatment of HCC with yttrium-90 (Y-90) glass microsphere-based transarterial radioembolization (TARE). Literature published between January 2019 and September 2021 was reviewed, discussed, and adjudicated by the Delphi method. Recommendations included in this updated document incorporate both the results of the literature review and the expert opinion and experience of members of the committee. Results: Committee discussion and consensus led to the expansion of recommendations to apply to five common clinical scenarios in patients with HCC to support more individualized efficacious treatment with Y-90 glass microspheres. Existing clinical scenarios were updated to reflect recent developments in dosimetry approaches and broader treatment paradigms evolving for patients presenting with HCC. Conclusion: Updated consensus recommendations are provided to guide clinical and dosimetric approaches for the use of Y-90 glass microsphere TARE in HCC, accounting for disease presentation, tumor biology, and treatment intent.
MeSH term(s)	Humans ; Carcinoma, Hepatocellular/pathology ; Yttrium Radioisotopes/therapeutic use ; Liver Neoplasms/diagnostic imaging ; Liver Neoplasms/radiotherapy ; Liver Neoplasms/drug therapy ; Microspheres ; Radiopharmaceuticals/therapeutic use ; Embolization, Therapeutic/methods ; Glass
Chemical Substances	Yttrium-90 (1K8M7UR6O1) ; Yttrium Radioisotopes ; Radiopharmaceuticals
Language	English
Publishing date	2022-09-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	8236-3
ISSN	1619-7089 ; 0340-6997 ; 1619-7070
ISSN (online)	1619-7089
ISSN	0340-6997 ; 1619-7070
DOI	10.1007/s00259-022-05956-w
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Zs.A 1227: Show issues

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Article ; Online: High density lipoprotein-associated miRNA is increased following Roux-en-Y gastric bypass surgery for severe obesity.

Ho, Jan Hoong / Ong, Kwok Leung / Cuesta Torres, Luisa F / Liu, Yifen / Adam, Safwaan / Iqbal, Zohaib / Dhage, Shaishav / Ammori, Basil J / Syed, Akheel A / Rye, Kerry-Anne / Tabet, Fatiha / Soran, Handrean

Journal of lipid research

2021 Volume 62, Page(s) 100043

Abstract: Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures ...

Abstract	Roux-en-Y gastric bypass (RYGB) is one of the most commonly performed weight-loss procedures, but how severe obesity and RYGB affect circulating HDL-associated microRNAs (miRNAs) remains unclear. Here, we aim to investigate how HDL-associated miRNAs are regulated in severe obesity and how weight loss after RYGB surgery affects HDL-miRNAs. Plasma HDLs were isolated from patients with severe obesity (n = 53) before and 6 and 12 months after RYGB by immunoprecipitation using goat anti-human apoA-I microbeads. HDLs were also isolated from 18 healthy participants. miRNAs were extracted from isolated HDL and levels of miR-24, miR-126, miR-222, and miR-223 were determined by TaqMan miRNA assays. We found that HDL-associated miR-126, miR-222, and miR-223 levels, but not miR-24 levels, were significantly higher in patients with severe obesity when compared with healthy controls. There were significant increases in HDL-associated miR-24, miR-222, and miR-223 at 12 months after RYGB. Additionally, cholesterol efflux capacity and paraoxonase activity were increased and intercellular adhesion molecule-1 (ICAM-1) levels decreased. The increases in HDL-associated miR-24 and miR-223 were positively correlated with an increase in cholesterol efflux capacity (r = 0.326, P = 0.027 and r = 0.349, P = 0.017, respectively). An inverse correlation was observed between HDL-associated miR-223 and ICAM-1 at baseline. Together, these findings show that HDL-associated miRNAs are differentially regulated in healthy participants versus patients with severe obesity and are altered after RYGB. These findings provide insights into how miRNAs are regulated in obesity before and after weight reduction and may lead to the development of novel treatment strategies for obesity and related metabolic disorders.
MeSH term(s)	Gastric Bypass
Language	English
Publishing date	2021-02-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80154-9
ISSN	1539-7262 ; 0022-2275
ISSN (online)	1539-7262
ISSN	0022-2275
DOI	10.1194/jlr.RA120000963
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Zs.A 175: Show issues

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Article ; Online: Immortalization and Characterization of Rat Lingual Keratinocytes in a High-Calcium and Feeder-Free Culture System Using ROCK Inhibitor Y-27632.

Chen, Zixing / He, Wenmeng / Leung, Thomas Chun Ning / Chung, Hau Yin

International journal of molecular sciences

2021 Volume 22, Issue 13

Abstract: ... Here, we investigated the effect of Y-27632, a Rho kinase (ROCK) inhibitor, on the immortalization and ... characterization of cultured rat lingual keratinocyte (RLKs). Three Y-27632-supplemented media were screened ... to visualize cytoskeleton dynamics and cell apoptosis following Y-27632 removal. Label-free proteomics, RT-PCR ...

Abstract	Cultured keratinocytes are desirable models for biological and medical studies. However, primary keratinocytes are difficult to maintain, and there has been little research on lingual keratinocyte culture. Here, we investigated the effect of Y-27632, a Rho kinase (ROCK) inhibitor, on the immortalization and characterization of cultured rat lingual keratinocyte (RLKs). Three Y-27632-supplemented media were screened for the cultivation of RLKs isolated from Sprague-Dawley rats. Phalloidin staining and TUNEL assay were applied to visualize cytoskeleton dynamics and cell apoptosis following Y-27632 removal. Label-free proteomics, RT-PCR, calcium imaging, and cytogenetic studies were conducted to characterize the cultured cells. Results showed that RLKs could be conditionally immortalized in a high-calcium medium in the absence of feeder cells, although they did not exhibit normal karyotypes. The removal of Y-27632 from the culture medium led to reversible cytoskeletal reorganization and nuclear enlargement without triggering apoptosis, and a total of 239 differentially expressed proteins were identified by proteomic analysis. Notably, RLKs derived from the non-taste epithelium expressed some molecular markers characteristic of taste bud cells, yet calcium imaging revealed that they rarely responded to tastants. Collectively, we established a high-calcium and feeder-free culture method for the long-term maintenance of RLKs. Our results shed some new light on the immortalization and differentiation of lingual keratinocytes.
MeSH term(s)	Amides/pharmacology ; Animals ; Calcium/metabolism ; Cell Culture Techniques ; Cells, Cultured ; Keratinocytes/drug effects ; Keratinocytes/metabolism ; Protein Kinase Inhibitors/pharmacology ; Pyridines/pharmacology ; Rats ; rho-Associated Kinases/antagonists & inhibitors
Chemical Substances	Amides ; Protein Kinase Inhibitors ; Pyridines ; Y 27632 (138381-45-0) ; rho-Associated Kinases (EC 2.7.11.1) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2021-06-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms22136782
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Article ; Online: Immortalization and Characterization of Rat Lingual Keratinocytes in a High-Calcium and Feeder-Free Culture System Using ROCK Inhibitor Y-27632

Zixing Chen / Wenmeng He / Thomas Chun Ning Leung / Hau Yin Chung

International Journal of Molecular Sciences, Vol 22, Iss 6782, p

2021 Volume 6782

Abstract: ... Here, we investigated the effect of Y-27632, a Rho kinase (ROCK) inhibitor, on the immortalization and ... characterization of cultured rat lingual keratinocyte (RLKs). Three Y-27632–supplemented media were screened ... to visualize cytoskeleton dynamics and cell apoptosis following Y-27632 removal. Label-free proteomics, RT-PCR ...

Abstract	Cultured keratinocytes are desirable models for biological and medical studies. However, primary keratinocytes are difficult to maintain, and there has been little research on lingual keratinocyte culture. Here, we investigated the effect of Y-27632, a Rho kinase (ROCK) inhibitor, on the immortalization and characterization of cultured rat lingual keratinocyte (RLKs). Three Y-27632–supplemented media were screened for the cultivation of RLKs isolated from Sprague–Dawley rats. Phalloidin staining and TUNEL assay were applied to visualize cytoskeleton dynamics and cell apoptosis following Y-27632 removal. Label-free proteomics, RT-PCR, calcium imaging, and cytogenetic studies were conducted to characterize the cultured cells. Results showed that RLKs could be conditionally immortalized in a high-calcium medium in the absence of feeder cells, although they did not exhibit normal karyotypes. The removal of Y-27632 from the culture medium led to reversible cytoskeletal reorganization and nuclear enlargement without triggering apoptosis, and a total of 239 differentially expressed proteins were identified by proteomic analysis. Notably, RLKs derived from the non-taste epithelium expressed some molecular markers characteristic of taste bud cells, yet calcium imaging revealed that they rarely responded to tastants. Collectively, we established a high-calcium and feeder-free culture method for the long-term maintenance of RLKs. Our results shed some new light on the immortalization and differentiation of lingual keratinocytes.
Keywords	lingual keratinocytes ; immortalization ; ROCK inhibitor ; Y-27632 ; high calcium ; feeder-free ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	610
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Metformin use was linked to hospitalization for acidosis at 6 y only in patients with eGFR < 30 mL/min/1.73 m2.

Leung, Alexander A / Padwal, Raj S

Annals of internal medicine

2018 Volume 169, Issue 8, Page(s) JC47

MeSH term(s)	Acidosis ; Acidosis, Lactic ; Cohort Studies ; ErbB Receptors ; Hospitalization ; Humans ; Hypoglycemic Agents ; Metformin
Chemical Substances	Hypoglycemic Agents ; Metformin (9100L32L2N) ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1)
Language	English
Publishing date	2018-11-15
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	336-0
ISSN	1539-3704 ; 0003-4819
ISSN (online)	1539-3704
ISSN	0003-4819
DOI	10.7326/ACPJC-2018-169-8-047
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Article ; Online: Mitochondrial DNA haplogroup Y is associated to Leigh syndrome in Chinese population.

Hao, Xiao-Dan / Yang, Yan-Ling / Tang, Nelson Leung Sang / Kong, Qing-Peng / Wu, Shi-Fang / Zhang, Ya-Ping

Gene

2013 Volume 512, Issue 2, Page(s) 460–463

Abstract: ... of 171 Chinese LS patients with that of 1597 controls. Our results show that haplogroup Y may increase ...

Abstract	Although Leigh syndrome (LS) is a well characterized clinical mitochondrial disorder; the exact mutation is not found in all cases and it is not clear whether matrilineal background has contributed to this disease. To address this issue, we extensively studied and compared the haplogroup composition of a sample of 171 Chinese LS patients with that of 1597 controls. Our results show that haplogroup Y may increase the risk of LS in Chinese by 2.867 fold (95% CI=1.135-7.240, P=0.020). Haplogroup B5 has also this trend (1.737 fold, 95% CI=0.961-3.139), but with a borderline P-value (P=0.065). Both haplogroups belong to macro-haplogroup N and share a common reverse mutation on nucleotide position 10398 (A10398G). In fact, the combined haplogroup N with 10398G is also associated with an increased risk for LS (OR=1.882, 95% CI=1.134-3.124, P=0.013).
MeSH term(s)	Adolescent ; Asian Continental Ancestry Group ; Base Sequence ; Child ; Child, Preschool ; China ; DNA, Mitochondrial/genetics ; Female ; Haplotypes ; Humans ; Infant ; Infant, Newborn ; Leigh Disease/epidemiology ; Leigh Disease/genetics ; Male ; Molecular Sequence Data ; Point Mutation
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2013-01-10
Publishing country	Netherlands
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2012.10.054
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Zs.A 1357: Show issues

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Article: Y-box-binding protein 1 contributes to IL-7-mediated survival signaling in B-cell precursor acute lymphoblastic leukemia.

Kariminia, Amina / Ivison, Sabine M / Leung, Vivian M / Sung, Susanna / Couto, Nicole / Rozmus, Jacob / Rolf, Nina / Narendran, Aru / Dunn, Sandra E / Reid, Gregor S D / Schultz, Kirk R

Oncology letters

2016 Volume 13, Issue 1, Page(s) 497–505

Abstract: Y-box-binding protein 1 (YB-1) is a regulatory protein that is associated with drug resistance and ...

Abstract	Y-box-binding protein 1 (YB-1) is a regulatory protein that is associated with drug resistance and relapse in solid tumors. As YB-1 mediates some of its activity through growth factor receptor signaling dysregulation, the present study compared the expression of YB-1 and interleukin 7 (IL-7) receptor α (IL-7Rα) in pediatric B-cell precursor (BCP) acute lymphoblastic leukemia (ALL) and normal BCP cells. The expression levels of IL-7Rα and YB-1 were higher in relapsed vs. diagnostic samples of primary BCP ALL; however, co-expression was also observed in a minor BCP cell population in samples from healthy donors. Functional crosstalk between YB-1 and IL-7R was detected: Overexpression of YB-1 increased surface levels of IL-7R in B cells, and the stimulation of BCP ALL cell lines and primary samples by IL-7 activated YB-1 by phosphorylation at S102 in a phosphatidylinositol 3-kinase-independent and MEK1/2-dependent manner. Targeted knockdown of YB-1 reduced IL-7-mediated protection against rapamycin, and an inhibitor of MEK1/2 potentiated rapamycin-mediated killing in the presence of IL-7. These data establish a novel link between two well-characterized pro-survival factors in acute leukemia, and suggest that YB-1 inhibition may represent a novel therapeutic strategy for increasing sensitivity to chemotherapy in patients with refractory acute B-cell leukemia.
Language	English
Publishing date	2016-11-28
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2573196-8
ISSN	1792-1082 ; 1792-1074
ISSN (online)	1792-1082
ISSN	1792-1074
DOI	10.3892/ol.2016.5437
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Article: Bone mineral density and calcium metabolism of Hong Kong Chinese postpartum women--a 1-y longitudinal study.

Chan, S M / Nelson, E A S / Leung, S S F / Cheng, J C Y

European journal of clinical nutrition

2005 Volume 59, Issue 7, Page(s) 868–876

Abstract: ... for at least 3 months and 14 formula feeding mothers aged 20-40 y were interviewed after delivery, 2 and 6 ...

Abstract	Objective: This paper reports longitudinal changes in bone mineral density (BMD), calcium homeostasis and dietary calcium intake in a group of Hong Kong breastfeeding women during the first year postpartum. Design and subjects: Nine mothers who breastfed exclusively or almost exclusively for at least 3 months and 14 formula feeding mothers aged 20-40 y were interviewed after delivery, 2 and 6 weeks, 3, 6 and 12 months postpartum. BMD at L2-L4 lumbar spine (LS), trochanter (Tro) and femoral neck (FN), serum intact parathyroid hormone (iPTH), serum bone-specific alkaline phosphatase (b-ALP), urinary deoxypyridinoline (Dpd), serum and urinary calcium (Ca) and phosphorus (P) and dietary intake of macronutrients were assessed. Results: Compared to the formula feeding group, BMD assessed at LS, Tro and FN decreased significantly in the breastfeeding group over the first 6 months, with rebound to approximate baseline values at 12 months for the latter two sites. Serum iPTH increased in both groups, whereas serum b-ALP was consistently higher in the breastfeeders. Urinary Ca and P excretion decreased early postpartum in both groups, but the breastfeeders had higher excretion at 3 and 6 months. Breastfeeding mothers consumed significantly more Ca than the formula feeding mothers in the early postpartum. Conclusions: Increased calcium requirement during early lactation is affected through mobilisation of bone and renal calcium conservation. Bone mineral loss during lactation is temporary. Further studies are warranted to investigate the effects of diet and other hormonal factors on the calcium homeostasis during lactation.
MeSH term(s)	Adult ; Bone Density/physiology ; Breast Feeding ; Calcium/metabolism ; Calcium/urine ; Calcium, Dietary/administration & dosage ; China/ethnology ; Diet ; Diet Records ; Female ; Hong Kong ; Humans ; Lactation/metabolism ; Longitudinal Studies ; Nutritional Requirements ; Phosphorus/metabolism ; Phosphorus/urine ; Postpartum Period/metabolism ; Postpartum Period/physiology
Chemical Substances	Calcium, Dietary ; Phosphorus (27YLU75U4W) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2005-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639358-5
ISSN	1476-5640 ; 0954-3007
ISSN (online)	1476-5640
ISSN	0954-3007
DOI	10.1038/sj.ejcn.1602148
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Zs.A 1045: Show issues

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Article: Mitochondrial DNA haplogroup Y is associated to Leigh syndrome in Chinese population

Hao, Xiao-Dan / Yang, Yan-Ling / Tang, Nelson Leung Sang / Kong, Qing-Peng / Wu, Shi-Fang / Zhang, Ya-Ping

Gene. 2013 Jan. 10, v. 512, no. 2

2013

Abstract: ... of 171 Chinese LS patients with that of 1597 controls. Our results show that haplogroup Y may increase ...

Abstract	Although Leigh syndrome (LS) is a well characterized clinical mitochondrial disorder; the exact mutation is not found in all cases and it is not clear whether matrilineal background has contributed to this disease. To address this issue, we extensively studied and compared the haplogroup composition of a sample of 171 Chinese LS patients with that of 1597 controls. Our results show that haplogroup Y may increase the risk of LS in Chinese by 2.867 fold (95% CI=1.135–7.240, P=0.020). Haplogroup B5 has also this trend (1.737 fold, 95% CI=0.961–3.139), but with a borderline P-value (P=0.065). Both haplogroups belong to macro-haplogroup N and share a common reverse mutation on nucleotide position 10398 (A10398G). In fact, the combined haplogroup N with 10398G is also associated with an increased risk for LS (OR=1.882, 95% CI=1.134–3.124, P=0.013).
Keywords	mitochondrial DNA ; mutation ; patients ; risk
Language	English
Dates of publication	2013-0110
Size	p. 460-463.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	391792-7
ISSN	1879-0038 ; 0378-1119
ISSN (online)	1879-0038
ISSN	0378-1119
DOI	10.1016/j.gene.2012.10.054
Database	NAL-Catalogue (AGRICOLA)

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Z 79/715: Show issues

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Book ; Online: Knowledge Justice : Disrupting Library and Information Studies through Critical Race Theory

Leung, Sofia Y. / López-McKnight, Jorge R.

2021

Keywords	Social discrimination & inequality ; Coding theory & cryptology ; Critical race theory ; Library and Information Studies ; Information science ; Libraries ; Archives ; Knowledge production ; Social justice ; Black ; Indigenous ; people of color ; white supremacy ; united states ; whiteness ; race ; racism ; care
Size	1 electronic resource (358 pages)
Publisher	The MIT Press
Publishing place	Cambridge
Document type	Book ; Online
Note	English ; Open Access
HBZ-ID	HT021291082
ISBN	9780262043502 ; 0262043505
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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