LIVIVO - Search results -

Search results

Result 1 - 10 of total 1159

Search options

Article ; Online: Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry.

Brazier, John

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2633, Page(s) 185–193

Abstract: Oligonucleotides are used in a variety of molecular biology techniques, from next-generation sequencing to genetic testing. Maintaining the sequence fidelity of synthetic oligonucleotides is critical to their use. This chapter describes the steps of ... ...

Abstract	Oligonucleotides are used in a variety of molecular biology techniques, from next-generation sequencing to genetic testing. Maintaining the sequence fidelity of synthetic oligonucleotides is critical to their use. This chapter describes the steps of solid phase oligonucleotide synthesis and purification, which enables the synthesis of oligonucleotides with specific sequences and high purity.
MeSH term(s)	Genetic Testing ; High-Throughput Nucleotide Sequencing ; Oligonucleotides/genetics ; Organophosphorus Compounds
Chemical Substances	phosphoramidite ; Oligonucleotides ; Organophosphorus Compounds
Language	English
Publishing date	2023-02-28
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3004-4_14
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Exploring health preference heterogeneity in the UK: Using the online elicitation of personal utility functions approach to construct EQ-5D-5L value functions on societal, group and individual level.

Schneider, Paul / Devlin, Nancy / van Hout, Ben / Brazier, John

Health economics

2024 Volume 33, Issue 5, Page(s) 894–910

Abstract: A new method has recently been developed for valuing health states, called 'Online elicitation of Personal Utility Functions' (OPUF). In contrast to established methods, such as time trade-off or discrete choice experiments, OPUF does not require ... ...

Abstract	A new method has recently been developed for valuing health states, called 'Online elicitation of Personal Utility Functions' (OPUF). In contrast to established methods, such as time trade-off or discrete choice experiments, OPUF does not require hundreds of respondents, but allows estimating utility functions for small groups and even at the individual level. In this study, we used OPUF to elicit EQ-5D-5L health state preferences from a (not representative) sample of the UK general population, and then compared utility functions on the societal-, group-, and individual level. A demo version of the survey is available at: https://eq5d5l.me. Data from 874 respondents were included in the analysis. For each respondent, we constructed a personal EQ-5D-5L value set. These personal value sets predicted respondents' choices in three hold-out discrete choice tasks with an accuracy of 78%. Overall, preferences varied greatly between individuals. However, PERMANOVA analysis showed that demographic characteristics explained only a small proportion of the variability between subgroups. While OPUF is still under development, it has important strengths: it can be used to construct value sets for patient reported outcome instruments such as EQ-5D-5L, while also allowing examination of underlying preferences in an unprecedented level of detail. In the future, OPUF could be used to complement existing methods, allowing valuation studies in smaller samples, and providing more detailed insights into the heterogeneity of preferences across subgroups.
MeSH term(s)	Humans ; Health Status ; Quality of Life ; Surveys and Questionnaires ; United Kingdom
Language	English
Publishing date	2024-01-20
Publishing country	England
Document type	Journal Article
ZDB-ID	1135838-5
ISSN	1099-1050 ; 1057-9230
ISSN (online)	1099-1050
ISSN	1057-9230
DOI	10.1002/hec.4805
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3555: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Development and Valuation of a Preference-Weighted Measure in Age-Related Macular Degeneration From the Vision Impairment in Low Luminance Questionnaire-A MACUSTAR Report.

Rowen, Donna / Carlton, Jill / Terheyden, Jan H / Finger, Robert P / Wickramasekera, Nyantara / Brazier, John

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research

2024

Abstract: Objectives: This study generates VILL-UI (Vision Impairment in Low Luminance - Utility Index), a preference-weighted measure (PWM) derived from the VILL-33 measure for use in patients with age-related macular degeneration (AMD) and valued to generate ... ...

Abstract	Objectives: This study generates VILL-UI (Vision Impairment in Low Luminance - Utility Index), a preference-weighted measure (PWM) derived from the VILL-33 measure for use in patients with age-related macular degeneration (AMD) and valued to generate United Kingdom and German preference weights. Methods: A PWM consists of a classification system to describe health and utility values for every state described by the classification. The classification was derived using existing data collected as part of the MACUSTAR study, a low-interventional study on AMD, conducted at 20 clinical sites across Europe. Items were selected using psychometric and Rasch analyses, published criteria around PWM suitability, alongside instrument developer views and concept elicitation work that informed VILL-33 development. An online discrete choice experiment (DCE) with duration of the health state was conducted with the United Kingdom and German public. Responses were modeled to generate utility values for all possible health states. Results: The classification system has 5 items across the 3 domains of VILL-33: reading and accessing information, mobility and safety, and emotional well-being. The DCE samples (United Kingdom: n = 1004, Germany: n = 1008) are broadly representative and demonstrate good understanding of the tasks. The final DCE analyses produce logically consistent and significant coefficients. Conclusions: This study enables responses to VILL-33 to be directly used to inform economic evaluation in AMD. The elicitation of preferences from both United Kingdom and Germany enables greater application of VILL-UI for economic evaluation throughout Europe. VILL-UI fills a gap in AMD in which generic preference-weighted measures typically lack sensitivity.
Language	English
Publishing date	2024-02-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	1471745-1
ISSN	1524-4733 ; 1098-3015
ISSN (online)	1524-4733
ISSN	1098-3015
DOI	10.1016/j.jval.2024.02.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5904: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Acalculous variant of Mirizzi syndrome: Imaging and clinical characteristics.

Figacz, Alexander / Brazier, Allan / Brazier, Joseph / Jamil, Laith H / Nandalur, Kiran / Al-Katib, Sayf

Clinical imaging

2022 Volume 94, Page(s) 62–70

Abstract: Purpose: Mirizzi Syndrome is a rare disease that causes biliary obstruction in the setting of an impacted stone in the gallbladder neck or Hartmann's Pouch which exerts mass effect on the common duct; however, we have noticed inflammatory biliary ... ...

Abstract	Purpose: Mirizzi Syndrome is a rare disease that causes biliary obstruction in the setting of an impacted stone in the gallbladder neck or Hartmann's Pouch which exerts mass effect on the common duct; however, we have noticed inflammatory biliary narrowing in the absence of an offending gallstone in the setting of acute cholecystitis. The purpose of this study is to report the clinical and MRCP findings in a series of 10 patients with this variant of Mirizzi Syndrome. Materials and methods: A search of our institution's PACS and electronic medical record identified 10 patients with a diagnosis of acute cholecystitis and narrowing of the common duct on imaging in the absence of an impacted gallstone. Imaging and clinical findings were confirmed by two board-certified abdominal radiologists. Results: All patients presented with abdominal pain and an average elevated total bilirubin of 3.0 mg/dL. Seven patients had MRCP findings of complete narrowing of the CBD. Nine patients had intrahepatic biliary ductal dilation. All nine patients with gadoliniumenhanced MRCP displayed biliary wall thickening with enhancement adjacent to the gallbladder. Nine patients underwent cholecystectomy, one patient underwent percutaneous cholecystostomy. Average bilirubin upon discharge was within normal limits at 0.9 mg/dL after intervention. Two patients had follow-up MRCP showing resolution of biliary narrowing. Conclusion: A variant of Mirizzi Syndrome occurs in the absence of an offending gallstone in the gallbladder neck or cystic duct to explain the biliary narrowing. We postulate that acute cholecystitis can cause a local inflammatory narrowing resulting in biliary obstruction.
Language	English
Publishing date	2022-12-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	1028123-x
ISSN	1873-4499 ; 0899-7071
ISSN (online)	1873-4499
ISSN	0899-7071
DOI	10.1016/j.clinimag.2022.11.017
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1469: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: All models are wrong, but the critical power model is useful.

Muniz-Pumares, Daniel / Maunder, Ed / Brazier, Jon / Hunter, Ben

European journal of applied physiology

2023 Volume 124, Issue 2, Page(s) 669–670

Language	English
Publishing date	2023-11-17
Publishing country	Germany
Document type	Letter
ZDB-ID	124793-1
ISSN	1439-6327 ; 1432-1025 ; 0301-5548 ; 1439-6319
ISSN (online)	1439-6327 ; 1432-1025
ISSN	0301-5548 ; 1439-6319
DOI	10.1007/s00421-023-05359-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.195: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Rapid, repeatable landscape-scale mapping of tree, hedgerow, and woodland habitats (THaW), using airborne LiDAR and spaceborne SAR data.

Luscombe, David J / Gatis, Naomi / Anderson, Karen / Carless, Donna / Brazier, Richard E

Ecology and evolution

2023 Volume 13, Issue 5, Page(s) e10103

Abstract: In the UK, tree, hedgerow, and woodland (THaW) habitats are key havens for biodiversity and support many related ecosystem services. The UK is entering a period of agricultural policy realignment with respect to natural capital and climate change, ... ...

Abstract	In the UK, tree, hedgerow, and woodland (THaW) habitats are key havens for biodiversity and support many related ecosystem services. The UK is entering a period of agricultural policy realignment with respect to natural capital and climate change, meaning that now is a critical time to evaluate the distribution, resilience, and dynamics of THaW habitats. The fine-grained nature of habitats like hedgerows necessitates mapping of these features at relatively fine spatial resolution-and freely available public archives of airborne laser scanning (LiDAR) data at <2 m spatial resolution offer a means of doing so within UK settings. The high cost of LiDAR prohibits use for regular monitoring of THaW change, but space-borne sensors such as Sentinel-1 Synthetic Aperture Radar (SAR at ca. 10 m resolution) can potentially meet this need once baseline distributions are established. We address two aims in this manuscript-(1) to rapidly quantify THaW across UK landscapes using LiDAR data and (2) to monitor canopy change intra- and inter-annually using SAR data. We show that workflows applied to airborne LiDAR data can deliver THaW baselines at 2 m resolution, with positional accuracy of >90%. It was also possible to combine LiDAR mapping data and Sentinel-1 SAR data to rapidly track canopy change through time (i.e., every 3 months) using, cloud-based processing via Google Earth Engine. The resultant toolkit is also provided as an open-access web app. The results highlight that whilst nearly 90% of the tallest trees (above 15 m) are captured within the National Forest Inventory (NFI) database only 50% of THaW with a canopy height range of 3-15 m are recorded. Current estimates of tree distribution neglect these finer-grained features (i.e., smaller or less contiguous THaW canopies), which we argue will account for a significant proportion of landscape THaW cover.
Language	English
Publishing date	2023-05-25
Publishing country	England
Document type	Journal Article
ZDB-ID	2635675-2
ISSN	2045-7758
ISSN	2045-7758
DOI	10.1002/ece3.10103
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Current Perspectives on the Assessment and Management of Gait Disorders in Parkinson's Disease.

Smith, Matthew D / Brazier, Danielle E / Henderson, Emily J

Neuropsychiatric disease and treatment

2021 Volume 17, Page(s) 2965–2985

Abstract: Gait dysfunction is a key defining feature of Parkinson's disease (PD), and is associated with symptoms of freezing and an increased risk of falls. In this narrative review, we cover the putative mechanisms of gait dysfunction in PD, the assessment of ... ...

Abstract	Gait dysfunction is a key defining feature of Parkinson's disease (PD), and is associated with symptoms of freezing and an increased risk of falls. In this narrative review, we cover the putative mechanisms of gait dysfunction in PD, the assessment of gait abnormalities, and the management of symptoms caused by the inherent difficulty in walking. Our understanding of the causes of gait problems in PD has progressed in recent times, moving from neurocognitive theory to correlates of affected neuronal pathways. In particular, this can be shown to correspond with abnormalities in responses to dual-task paradigms and dysfunction in cholinergic signaling. Great progress has been made in the sophistication and precision of gait assessment; however, it has firmly remained in the research domain. There is significant momentum behind wearable technologies that can be used by patients in their own environment, acting as digital biomarkers that can not only reflect progression but also independently discriminate PD from non-PD individuals. The treatment of gait dysfunction has historically relied on physical therapies and training combined with a view to mitigating the impact of such consequences as falls. Pharmacological therapies that are the mainstay of treatment in PD have tended to address symptoms like bradykinesia; however, optimization of dopaminergic therapies likely has a positive effect on quality of gait. Other targets have been assessed with the goal of improving gait, of which medications that improve cholinergic signaling appear most promising. Neuromodulation techniques are increasingly used in the form of deep-brain stimulation; however, standard targets, such as the globus pallidus interna, have a modest effect on gait. Considerable benefit has been seen through targeting the pedunculopontine nucleus, and a dual-target approach may be warranted. Stimulation of the spinal cord and brain through direct or magnetic approaches has been assessed, but requires further evidence.
Language	English
Publishing date	2021-09-21
Publishing country	New Zealand
Document type	Journal Article ; Review
ZDB-ID	2186503-6
ISSN	1178-2021 ; 1176-6328
ISSN (online)	1178-2021
ISSN	1176-6328
DOI	10.2147/NDT.S304567
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 6068: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Turner’s 1836 Alpine Crossing and the Little Ice Age Portrayed in Val Veny and Val Ferret; a Geography of the Sublime

Martin P. Kirkbride / Susan P. Mains / Vanessa Brazier

Revue de Géographie Alpine, Vol 111, Iss

2023 Volume 2

Abstract: The English artist J.M.W. Turner made his second visit to upper Val d’Aosta in 1836, after ...

Abstract	The English artist J.M.W. Turner made his second visit to upper Val d’Aosta in 1836, after which he produced several significant paintings. Here, we accurately locate two of these works and relate them to the climatic and geomorphological environment of the time. We combine critical geomorphological and cultural geographic analyses to derive a more holistic appraisal of Turner’s artistic motivations and methods in his Alpine works. The first is a watercolour of Mont Rouge de Peuterey from across the Glacier du Miage. The second is a major oil painting re-imagining of the 1717 Triolet disaster which conflates the complex geomorphological processes of this rock-ice avalanche and subsequent outburst flood into a single dramatic scene. Turner’s visit coincided with the mid-nineteenth century maximum advance of glaciers in the region allowing him to witness dynamic geomorphological processes in close proximity. The precise viewpoints of these scenes demonstrate that Turner spent some time in the area to seek out preferred locations and that he was aware of considerable details of local history. The paintings speak of an immersion in the environment of the time, and their study yields fruitful insights into the complex relationships between geography and landscape art.
Keywords	J.M.W. Turner ; Landscape painting ; Val d’Aosta ; Little Ice Age ; Triolet rock avalanche ; Geography. Anthropology. Recreation ; G ; Physical geography ; GB3-5030
Subject code	910
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Institut de Géographie Alpine
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: La traversée alpine de Turner en 1836 et le petit âge glaciaire dépeints dans le Val Veny et le Val Ferret (massif du Mont-Blanc)

Martin P. Kirkbride / Susan P. Mains / Vanessa Brazier

Revue de Géographie Alpine, Vol 111, Iss

2023 Volume 2

Abstract: L’artiste anglais J.M.W. Turner (1775-1851) a réalisé sa deuxième visite dans le haut Val d’Aoste en 1836. Elle lui inspira plusieurs tableaux importants. Ici, nous localisons avec précision deux de ces œuvres et les relions à l’environnement climatique ... ...

Abstract	L’artiste anglais J.M.W. Turner (1775-1851) a réalisé sa deuxième visite dans le haut Val d’Aoste en 1836. Elle lui inspira plusieurs tableaux importants. Ici, nous localisons avec précision deux de ces œuvres et les relions à l’environnement climatique et géomorphologique de l’époque. Nous combinons des analyses géomorphologiques et géographiques culturelles pour proposer une évaluation plus holistique des motivations artistiques et des méthodes de Turner dans ses œuvres alpines. La première œuvre est une aquarelle présentant le mont Rouge de Peuterey (2941 m) à proximité du glacier du Miage (Val Veny). La seconde est une peinture à l’huile majeure interprétant la catastrophe du Triolet (3870 m) de 1717 (Val Ferret). Pour cette dernière, l’artiste a combiné en une seule scène dramatique les processus géomorphologiques complexes que sont une avalanche rocheuse impliquant également de la glace et l’inondation qui a suivi. La visite de Turner dans la région a coïncidé avec l’avancée maximale des glaciers au milieu du xixe siècle, ce qui lui a permis d’être confronté à des processus géomorphologiques d’ampleur. Les points de vue précis de ces deux scènes, déterminés ici pour la première fois, montrent que Turner a passé un certain temps dans la région pour rechercher les endroits les plus favorables et qu’il connaissait certains éléments importants de l’histoire locale. Les peintures font état d’une immersion dans l’environnement de l’époque, et leur étude donne un aperçu fructueux des relations complexes qui existent entre la géographie et l’art du paysage en montagne.
Keywords	J.M.W. Turner ; peinture de paysage ; Val d’Aoste ; Petit âge glaciaire ; avalanche rocheuse du Triolet ; Geography. Anthropology. Recreation ; G ; Physical geography ; GB3-5030
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Institut de Géographie Alpine
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Valuing SF-6Dv2 in Australia Using an International Protocol.

Mulhern, Brendan / Norman, Richard / Brazier, John

PharmacoEconomics

2021 Volume 39, Issue 10, Page(s) 1151–1162

Abstract: Background: The SF-6Dv2 is an updated version of the SF-6D, with improved consistency and dimension descriptors, and value sets are required. The aim of this study was to estimate an SF-6Dv2 value set for Australia using an international protocol, while ...

Abstract	Background: The SF-6Dv2 is an updated version of the SF-6D, with improved consistency and dimension descriptors, and value sets are required. The aim of this study was to estimate an SF-6Dv2 value set for Australia using an international protocol, while secondary aims were to compare Australian and UK values and to understand heterogeneity. Methods: The study design was based on the SF-6Dv2 valuation protocol, which involved the administration of two different discrete choice experiment (DCE) tasks. The first task presented pairs of SF-6Dv2 health states with duration (Design 1), and the second task presented triplets that appended 'immediate death' to the health state pairs (Design 2). Respondents completed 12 choice sets online (10 from Design 1; 2 from Design 2). Value sets were estimated for Design 1 separately and combining Designs 1 and 2 using a variety of logit model specifications. Value set characteristics were compared descriptively with the UK model. Results: The sample included 3001 Australians. A range of ordered and consistent models were estimated. The models only including Design 1 data resulted in a relatively wider utility range. The value range was reduced by an interaction added when the health state included a dimension with a severe level. The model matched with the UK value set resulted in a larger utility range and consistent ordering of the most important dimensions. Conclusion: The model including the Design 1 data and moderating interaction was chosen for the Australian value set. This allows the SF-6Dv2 to be used in health technology assessment decision making in Australia.
MeSH term(s)	Australia ; Health Status ; Humans ; Logistic Models ; Quality of Life ; Surveys and Questionnaires
Language	English
Publishing date	2021-07-12
Publishing country	New Zealand
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1100273-6
ISSN	1179-2027 ; 1170-7690
ISSN (online)	1179-2027
ISSN	1170-7690
DOI	10.1007/s40273-021-01043-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 3579: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito