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  1. Article ; Online: Effect of potential microplastics in sewage effluent on Nile Tilapia and photocatalytic remediation with zinc oxide nanoparticles.

    Alanazi, Manal Qayyadh / Virk, Promy / Alterary, Seham Soliman / Awad, Manal / Ahmad, Zubair / Albadri, Abdulrehman M / Ortashi, KalidM / Ahmed, Mohieldeen Mahgoub A / Ali Yousef, Mohamed Ismail / Elobeid, Mai / Al-Qahtani, Ebtesam Abdullah

    Environmental pollution (Barking, Essex : 1987)

    2023  Volume 332, Page(s) 121946

    Abstract: The aim of the present study was a qualitative assessment of potential microplastics (MPs) in the sewage effluent collected from a local sewage treatment plant located in Riyadh City, Saudi Arabia. The composite samples of domestic sewage effluent were ... ...

    Abstract The aim of the present study was a qualitative assessment of potential microplastics (MPs) in the sewage effluent collected from a local sewage treatment plant located in Riyadh City, Saudi Arabia. The composite samples of domestic sewage effluent were subjected to UV (ultraviolet) light-induced zinc oxide nanoparticles (ZnONPs) mediated photocatalysis. The first phase of the study included the synthesis of the ZnONPs with an extensive characterization. The synthesized nanoparticles were 220 nm in size with a characteristic spherical/hexagonal shape. These NPs were then used at three different concentrations (10 mM, 20 mM, and 30 mM) for the UV light-induced photocatalysis. A shift in the Raman spectra on photodegradation mirrored the surface changes of the functional groups shown by the FTIR spectra; presence of functional groups containing oxygen and C-C bonds associated with oxidation and chain scission. SEM micrographs showed photodegraded particles. Complementary elemental maps from the EDS analysis showed the presence of C, O, and Cl suggesting the potential presence of MPs. The O/C ratio was used to assess potential oxidation degree. In addition, an evaluation of the toxicological effects of the potential MPs in the sewage effluent on Nile tilapia (Oreochromis niloticus) exposed to the effluent at two concentrations (50% and 75%) elicited a marked response in the endpoints evaluated; EROD activity, MDA (malondialdehyde), 8-oxo-2'-deoxyguanosine levels in and AChE (acetylcholinesterase) activity in the brain. Thus, the key results provide new insights into the use of clean technologies to combat global MP pollution in aquatic ecosystems.
    MeSH term(s) Animals ; Cichlids/metabolism ; Microplastics/metabolism ; Zinc Oxide/chemistry ; Plastics/metabolism ; Sewage ; Acetylcholinesterase/metabolism ; Ecosystem ; Nanoparticles
    Chemical Substances Microplastics ; Zinc Oxide (SOI2LOH54Z) ; Plastics ; Sewage ; Acetylcholinesterase (EC 3.1.1.7)
    Language English
    Publishing date 2023-06-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 280652-6
    ISSN 1873-6424 ; 0013-9327 ; 0269-7491
    ISSN (online) 1873-6424
    ISSN 0013-9327 ; 0269-7491
    DOI 10.1016/j.envpol.2023.121946
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Putative anti-proliferative effect of Indian mustard (Brassica juncea) seed and its nano-formulation

    Virk Promy / Awad Manal A. / Alanazi Meznah M. / Hendi Awatif A. / Elobeid Mai / Ortashi Khalid M. / Alrowaily Albandari W. / Bahlool Taghreed / Aouaini Fatma

    Green Processing and Synthesis, Vol 12, Iss 1, Pp 685-

    2023  Volume 850

    Abstract: Over the past few decades, nanotechnology has shown promising prospects in biomedicine and has a proven impact on enhancing therapeutics by facilitating drug delivery. The present study brings an amalgamation of nanoscience and “clean technology” by ... ...

    Abstract Over the past few decades, nanotechnology has shown promising prospects in biomedicine and has a proven impact on enhancing therapeutics by facilitating drug delivery. The present study brings an amalgamation of nanoscience and “clean technology” by fabricating nature-friendly nanoparticles (NPs) sans the use of chemical surfactants using Indian mustard seed, Brassica juncea L. The as-synthesized NPs were characterized to assess their average size, crystallinity, morphology, and constituent functional groups through conventional techniques: dynamic light scattering (DLS), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR). The NPs were crystalline in nature and exhibited a mean size of 205.5 nm (PDI of 0.437) being primarily polygonal in shape. Additionally, the therapeutic efficacy of the green NPs was evaluated based on their cytotoxic effect against two human cancer lines, MCF-7 and HepG-2. Both the NPs and the bulk seeds showed a dose-dependent cytotoxic effect. However, an assessment of the antiproliferative/cytotoxic potential of the green NPs versus the bulk seeds showed that the NPs were relatively more efficacious on both cell lines. Taken together, the mustard seed NPs could be potential nutraceuticals considering the green credential in their mode of biosynthesis.
    Keywords green synthesis ; nanoformulation ; indian mustard seed ; brassica juncea l ; anti-proliferative effect ; Chemistry ; QD1-999
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher De Gruyter
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: In Silico Studies on Zinc Oxide Based Nanostructured Oil Carriers with Seed Extracts of

    Hendi, Awatif A / Virk, Promy / Awad, Manal A / Elobeid, Mai / Ortashi, Khalid M O / Alanazi, Meznah M / Alkallas, Fatemah H / Almoneef, Maha Mohammad / Abdou, Mohammed Aly

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 13

    Abstract: Coming into the second year of the pandemic, the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants continue to be a serious health hazard globally. A surge in the omicron wave, despite the discovery of the vaccines, has shifted the ... ...

    Abstract Coming into the second year of the pandemic, the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants continue to be a serious health hazard globally. A surge in the omicron wave, despite the discovery of the vaccines, has shifted the attention of research towards the discovery and use of bioactive compounds, being potential inhibitors of the viral structural proteins. The present study aimed at the green synthesis of zinc oxide (ZnO) nanoparticles with seed extracts of
    MeSH term(s) COVID-19/drug therapy ; Cysteine Endopeptidases/chemistry ; Humans ; Ligands ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Nanostructures ; Nigella sativa/metabolism ; Peptide Hydrolases/metabolism ; Pimpinella ; Plant Extracts/pharmacology ; Protease Inhibitors/chemistry ; SARS-CoV-2 ; Seeds/metabolism ; Viral Nonstructural Proteins/metabolism ; Zinc Oxide/pharmacology
    Chemical Substances Ligands ; Plant Extracts ; Protease Inhibitors ; Viral Nonstructural Proteins ; Peptide Hydrolases (EC 3.4.-) ; Cysteine Endopeptidases (EC 3.4.22.-) ; Zinc Oxide (SOI2LOH54Z)
    Language English
    Publishing date 2022-07-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27134301
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Green synthesis of Moringa oleifera leaf nanoparticles and an assessment of their therapeutic potential

    Promy Virk / Manal A. Awad / Sarah Saleh Abdu-llah Alsaif / Awatif A. Hendi / Mai Elobeid / Khalid Ortashi / Rabia Qindeel / Manal F. El-Khadragy / Hany M. Yehia / Mohamed Ferkry Serag EL-DIN / Hatem Ali Salama

    Journal of King Saud University: Science, Vol 35, Iss 3, Pp 102576- (2023)

    2023  

    Abstract: The present study centered around the prospect of bio fabricating nanoparticles using Moringa oleifera leaf and investigating their therapeutic potential with an assessment of the antioxidant status. Synthesis of stable ‘green’ nanoparticles of Moringa ... ...

    Abstract The present study centered around the prospect of bio fabricating nanoparticles using Moringa oleifera leaf and investigating their therapeutic potential with an assessment of the antioxidant status. Synthesis of stable ‘green’ nanoparticles of Moringa leaf extract is successfully reported with an extensive characterization. The average particle size and polydispersity index (PDI) of the nanoparticles obtained by Dynamic light scattering (DLS) analysis were 141.6 nm and a polydispersity index (PDI) of 0.32 respectively. The UV–visible spectroscopy (UV–vis) revealed a strong red absorbance peak at 664 nm,characteristic of chlorophyll. Electron micrographs confirmed that particles were in the nano range and were spherical with minimal agglomeration. The constituent functional groups were assessed by Fourier transformed infrared spectroscopy (FTIR). The X-ray diffractograms of reflected both the amorphous and crystalline domains.Furthermore, the phytochemical screening showed a higher phenolic/ flavonoid content of the Moringa nanoparticles (NMo) in comparison to the bulk Moringa leaf extract(Mo). These results corresponded to a potent antioxidant status of the NMo which was assessed by three complementary antioxidant assays. The therapeutic potential of the nanoparticles was evaluated based on in vitro anti-diabetic assays and cytotoxicity assay on human cancer cell lines (MCF-7 and HepG-2). The key findings suggest a more profound therapeutic efficacy of NMo than the Moringa leaf extract.
    Keywords Moringa oleifera ; Green synthesis ; Antioxidant ; Phytochemical profile ; Anti-diabetic ; Anticancer ; Science (General) ; Q1-390
    Subject code 500
    Language English
    Publishing date 2023-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Synthesis and characterization of noble metal/metal oxide nanoparticles and their potential antidiabetic effect on biochemical parameters and wound healing

    Elobeid Mai A. / Awad Manal A. / Virk Promy / Ortashi Khalid M. / Merghani Nada M. / Asiri Atheer M. / Bashir Emadeldin Abdeljabar Ali

    Green Processing and Synthesis, Vol 11, Iss 1, Pp 106-

    2022  Volume 115

    Abstract: The study assessed the antidiabetic effect of Solenostemma argel and its nanoformulations with silver/gold nanocomposites (CNPs), zinc oxide nanoparticles (ZnONPs), and metaformin drug. Experimental groups consisted of normal control, diabetic control, ... ...

    Abstract The study assessed the antidiabetic effect of Solenostemma argel and its nanoformulations with silver/gold nanocomposites (CNPs), zinc oxide nanoparticles (ZnONPs), and metaformin drug. Experimental groups consisted of normal control, diabetic control, and four diabetic groups treated with metformin, CNPs, ZnONPs, and bulk argel leaf extract (So-argel). Transmission electron microscopy characterization showed that the synthesized CNPs and ZnONPs were of variable sizes and dimensions and were quasi-spherical in shape. Particle sizes measured by dynamic light scattering were 106 and 139 nm for CNPs and ZnONPs, respectively. Also, the polydispersity index values were 0.473 and 0.269 for CNPs and ZnONPs, respectively. The biochemical parameters were as follows: the group treated with bulk So-argel (105.00 ± 4.041 mg·dL−1) and CNPs (109.00 ± 8.373 mg·dL−1) showed a more profound anti-hyperglycemic effect and were comparable to the control (88.40 ± 2.249). Liver and kidney functions (p ≤ 0.05) improved with So-argel and its nanoformulations compared to metformin. However, bulk argel (170.33 ± 20.431 and 38.00 ± 3.05 U·L−1) and the nanocomposite (228.33 ± 11.464 and 48.00 ± 5.291 U·L−1) were efficacious in lowering serum levels of liver enzymes (AST and ALT, respectively). No significant difference was observed between urea levels. Nevertheless, bulk So-argel (0.26 ± 0.007) and CNPs (0.24 ± 0.018) were more effective than ZnONPs (0.41 ± 0.289) on serum creatinine. Nanotreatment exhibited a reduction in lesions size/healing. Overall, nanoparticles may offer a safe potential for Type 2 diabetes management.
    Keywords a diabetic lesion ; diabetes mellitus ; nanoparticles ; solenostemma argel ; Chemistry ; QD1-999
    Subject code 571
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher De Gruyter
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: In Silico Studies on Zinc Oxide Based Nanostructured Oil Carriers with Seed Extracts of Nigella sativa and Pimpinella anisum as Potential Inhibitors of 3CL Protease of SARS-CoV-2

    Awatif A. Hendi / Promy Virk / Manal A. Awad / Mai Elobeid / Khalid M. O. Ortashi / Meznah M. Alanazi / Fatemah H. Alkallas / Maha Mohammad Almoneef / Mohammed Aly Abdou

    Molecules, Vol 27, Iss 13, p

    2022  Volume 4301

    Abstract: Coming into the second year of the pandemic, the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants continue to be a serious health hazard globally. A surge in the omicron wave, despite the discovery of the vaccines, has shifted the ... ...

    Abstract Coming into the second year of the pandemic, the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants continue to be a serious health hazard globally. A surge in the omicron wave, despite the discovery of the vaccines, has shifted the attention of research towards the discovery and use of bioactive compounds, being potential inhibitors of the viral structural proteins. The present study aimed at the green synthesis of zinc oxide (ZnO) nanoparticles with seed extracts of Nigella sativa and Pimpinella anisum —loaded nanostructured oil carriers (NLC)—using a mixture of olive and black seed essential oils. The synthesized ZnO NLC were extensively characterized. In addition, the constituent compounds in ZnO NLC were investigated as a potential inhibitor for the SARS-CoV-2 main protease (3CLpro or Mpro) where 27 bioactive constituents, along with ZnO in the nanostructure, were subjected to molecular docking studies. The resultant high-score compounds were further validated by molecular dynamics simulation. The study optimized the compounds dithymoquinone, δ-hederin, oleuropein, and zinc oxide with high docking energy scores (ranging from −7.9 to −9.9 kcal/mol). The RMSD and RMSF data that ensued also mirrored these results for the stability of proteins and ligands. RMSD and RMSF data showed no conformational change in the protein during the MD simulation. Histograms of every simulation trajectory explained the ligand properties and ligand–protein contacts. Nevertheless, further experimental investigations and validation of the selected candidates are imperative to take forward the applicability of the nanostructure as a potent inhibitor of COVID-19 (Coronavirus Disease 2019) for clinical trials.
    Keywords coronavirus ; Nigella sativa ; Pimpenella anisum ; molecular docking ; molecular dynamic simulation ; zinc oxide nanoparticles ; Organic chemistry ; QD241-441
    Subject code 540
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Attenuation of di(2-ethylhexyl)phthalate-induced hepatic and renal toxicity by naringin nanoparticles in a rat model

    Al-Qahtani Malak Abdullah / Virk Promy / Awad Manal / Elobeid Mai / Alotaibi Khawlah Sultan

    Green Processing and Synthesis, Vol 12, Iss 1, Pp 2-

    2023  Volume 25

    Keywords di(2-ethylhexyl)phthalate ; naringin ; nanoparticles ; oxidative stress ; inflammation ; Chemistry ; QD1-999
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher De Gruyter
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Protective effect of probiotic bacteria and its nanoformulation against cadmium-induced oxidative stress in male Wistar rat

    Amenah Mohmmad Monadi Al-Enazi / Promy Virk / Awatif Hindi / Manal. A. Awad / Mai Elobeid / Rabia Qindeel

    Journal of King Saud University: Science, Vol 32, Iss 7, Pp 3045-

    2020  Volume 3051

    Abstract: The present study assessed the ameliorative potential of a commercial probiotic, Protexin® and its nanoformulation against cadmium (Cd) toxicity in Wistar rats. The ‘green’ synthesized nanoparticles had a size of 170 nm (PDI 0.527) with spherical and ... ...

    Abstract The present study assessed the ameliorative potential of a commercial probiotic, Protexin® and its nanoformulation against cadmium (Cd) toxicity in Wistar rats. The ‘green’ synthesized nanoparticles had a size of 170 nm (PDI 0.527) with spherical and tubular bacillary shapes. Rats were exposed to 70 ppm of cadmium chloride hydrate for 35 days. Group I and V were negative and positive control respectively. Groups II, III, and IV were exposed to Cd. The Cd exposed groups, III and IV were treated with the probiotic and nanoprobiotic (1 ml containing 2 × 108cfu) respectively. Cd intoxication led to significant bioaccumulation of cadmium in liver and kidney. A significant increase in the serum malondialdehyde (MDA) and 8 -hydroxydeoxyguanosine (8-OHdG) levels was observed. The metallothionien (MT) and reduced glutathione (GSH) levels in the liver were significantly increased. The advance oxidation protein products (AOPP) and methylglyoxal (MG) levels were also elevated. Treatment with probiotic and its nanoparticles markedly reversed the Cd induced alterations in the parameters. The nanoformulation was more profound in reducing the (GSH) and MT levels in the liver and 8-OHdG levels in the serum. The key findings suggest that a nutritional intervention in the form of probiotic supplementation is a safe and efficacious remediation against heavy metal toxicity/oxidative stress.
    Keywords Cadmium ; Probiotics ; Nanoparticles ; Oxidative stress ; Science (General) ; Q1-390
    Subject code 500
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Plausible effect of hesperetin and nano-hesperetin against bisphenol-A induced hepatoxicity in a rat model

    Khawlah Sultan Alotaibi / Mai Elobeid / Promy Virk / Manal Ahmed Awad / Malak Abdullah Al-Qahtani / Doaa Mohamed Elnagar

    Arabian Journal of Chemistry, Vol 17, Iss 2, Pp 105563- (2024)

    2024  

    Abstract: Bisphenol A(BPA) is a non-persistent, anthropic compound and continues to be ubiquitous in the environment. Since BPA is estrogenic in nature, its exposure induces adverse cellular responses associated with oxidative stress and inflammation. Hesperetin, ... ...

    Abstract Bisphenol A(BPA) is a non-persistent, anthropic compound and continues to be ubiquitous in the environment. Since BPA is estrogenic in nature, its exposure induces adverse cellular responses associated with oxidative stress and inflammation. Hesperetin, a major flavonoid present in lemons and sweet oranges exhibits a broad spectrum of pharmacological effects attributed to its potent anti-inflammatory and antioxidant status. The current study reports the ‘green’ synthesis of nano hesperetin (NHsp) followed by characterization that included; Fourier Transform Infrared spectroscopy (FTIR), X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Dynamic Light Scattering (DLS). In accordance with the findings, the mean size of the synthesized nanoparticles was 139.9 nm with a PDI of 0.362. The morphotype was primarily spherical and rod shaped. This was followed by a comparative assessment of the ameliorative efficacy of bulk and nano hesperetin against BPA-induced hepatotoxicity in rat. The findings showed that post-exposure to 250 mg/kg of BPA for 21 days significantly enhanced the pro-inflammatory cytokines (IL-6, IL-8, IL-1β, TNF-α), in the serum with a concomitant upregulation in the mRNA expression of Nrf 2(Nuclear factor erythroid 2-related factor) factor in the liver. Treatment with both bulk hesperetin (Hst) and nano hesperetin (NHst) reduced the levels of cytokines and expression of hepatic Nrf2 factor. This was paralleled by the modulatory effects on the histology of liver and kidney which were reversed on treatment. Taken together, the nano hesperetin was more efficacious in alleviating the BPA-induced toxicity. Thus,hesperetin and its nano formulation could be potentially promising nutraceuticals in medicine and industry.
    Keywords Bisphenol A ; Hesperetin ; Oxidative stress ; Inflammation ; Nanoparticles ; Green synthesis ; Chemistry ; QD1-999
    Subject code 500
    Language English
    Publishing date 2024-02-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Putative environmental-endocrine disruptors and obesity: a review.

    Elobeid, Mai A / Allison, David B

    Current opinion in endocrinology, diabetes, and obesity

    2008  Volume 15, Issue 5, Page(s) 403–408

    Abstract: Purpose of review: There has been a substantial increase in the prevalence of obesity in the last several decades. Recent evidence suggests that endocrine-disrupting chemicals, for example halogenated aromatic hydrocarbons, may cause perturbations in ... ...

    Abstract Purpose of review: There has been a substantial increase in the prevalence of obesity in the last several decades. Recent evidence suggests that endocrine-disrupting chemicals, for example halogenated aromatic hydrocarbons, may cause perturbations in endogenous hormonal regulation and alter other mechanisms involved in weight homeostasis, which may lead to weight gain by increased volume of adipose tissue. Synthetic chemicals derived from industrial processes are suspected to play a contributory role. Yet of the approximately 70,000 documented synthetic chemicals, few have been examined to determine their effects on the endocrine system.
    Recent findings: The present study examines prior laboratory, epidemiological and experimental research findings. Data demonstrate migration of endocrine disruptors in the environment and are beginning to catalogue their effects on adiposity. We present postulated relationships between these chemicals, their mechanisms of action, and the obesity epidemic.
    Summary: Endocrine disruptors may adversely impact human and environmental health by altering the physiological control mechanism. Obesity, which is known to increase medical costs and reduce quality and length of life, may be increasing as a function of endocrine disruptor exposure. This merits concern among scientists and public health officials and warrants additional vigorous research in this area.
    MeSH term(s) Animals ; Endocrine Disruptors/adverse effects ; Endocrine Disruptors/chemistry ; Environmental Exposure/adverse effects ; Environmental Pollutants/adverse effects ; Hormones ; Humans ; Obesity/epidemiology ; Obesity/etiology
    Chemical Substances Endocrine Disruptors ; Environmental Pollutants ; Hormones
    Language English
    Publishing date 2008-09-04
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2272017-0
    ISSN 1752-2978 ; 1752-296X
    ISSN (online) 1752-2978
    ISSN 1752-296X
    DOI 10.1097/MED.0b013e32830ce95c
    Database MEDical Literature Analysis and Retrieval System OnLINE

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