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Article ; Online: Alkali-assisted engineering of ultrathin graphite phase carbon nitride nanosheets with carbon vacancy and cyano group for significantly promoting photocatalytic hydrogen peroxide generation under visible light: Fast electron transfer channel.

Shi, Jianhui / Wang, Hui / Nie, Jianhui / Yang, Tiantian / Ju, Chenke / Pu, Kaikai / Shi, Jiating / Zhao, Ting / Li, Houfen / Xue, Jinbo

Journal of colloid and interface science

2023 Volume 643, Page(s) 47–61

Abstract: Exfoliating bulk graphite phase carbon nitride (g- ... ...

Abstract	Exfoliating bulk graphite phase carbon nitride (g-C
Language	English
Publishing date	2023-04-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	241597-5
ISSN	1095-7103 ; 0021-9797
ISSN (online)	1095-7103
ISSN	0021-9797
DOI	10.1016/j.jcis.2023.03.209
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: LaserNet: a method of laser stripe center extraction under non-ideal conditions.

Shang, Jiawei / Chen, Yuzhou / Nie, Jianhui

Applied optics

2023 Volume 62, Issue 13, Page(s) 3387–3397

Abstract: The extraction of the center of a laser stripe is a key step in line-structure measurement, where noise interference and changes in the surface color of an object are the main factors affecting extraction accuracy. To obtain sub-pixel level center ... ...

Abstract	The extraction of the center of a laser stripe is a key step in line-structure measurement, where noise interference and changes in the surface color of an object are the main factors affecting extraction accuracy. To obtain sub-pixel level center coordinates under such non-ideal conditions, we propose LaserNet, a novel deep learning-based algorithm, to the best of our knowledge, which consists of a laser region detection sub-network and a laser position optimization sub-network. The laser region detection sub-network is used to determine potential stripe regions, and the laser position optimization sub-network uses the local image of these regions to obtain the accurate center position of the laser stripe. The experimental results show that LaserNet can eliminate noise interference, handle color changes, and give accurate results under non-ideal conditions. The three-dimensional reconstruction experiments further demonstrate the effectiveness of the proposed method.
Language	English
Publishing date	2023-05-01
Publishing country	United States
Document type	Journal Article
ISSN	1539-4522
ISSN (online)	1539-4522
DOI	10.1364/AO.486107
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Assays Based on Pseudotyped Viruses.

Nie, Jianhui / Wu, Xueling / Wang, Youchun

Advances in experimental medicine and biology

2023 Volume 1407, Page(s) 29–44

Abstract: Pseudotyped viruses are more and more widely used in virus research and the evaluation of antiviral products because of their high safety, simple operation, high accessibility, ease in achieving standardization, and high throughput. The development of ... ...

Abstract	Pseudotyped viruses are more and more widely used in virus research and the evaluation of antiviral products because of their high safety, simple operation, high accessibility, ease in achieving standardization, and high throughput. The development of measures based on pseudotyped virus is closely related to the characteristics of viruses, and it is also necessary to follow the principles of assay development. Only in the process of method development, where the key parameters that affect the results are systematically optimized and the preliminary established method is fully validated, can the accuracy, reliability, and repeatability of the test results be ensured. Only the method established on this basis can be transferred to different laboratories and make the results of different laboratories comparable. This paper summarizes the specific aspects and general principles in the development of assays based on pseudotyped virus, which is of reference value for the development of similar methods.
MeSH term(s)	Viral Pseudotyping ; Reproducibility of Results ; Antiviral Agents ; Reference Values
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2023-03-15
Publishing country	United States
Document type	Journal Article
ISSN	2214-8019 ; 0065-2598
ISSN (online)	2214-8019
ISSN	0065-2598
DOI	10.1007/978-981-99-0113-5_2
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Pseudotyped Virus for Papillomavirus.

Wu, Xueling / Nie, Jianhui / Wang, Youchun

Advances in experimental medicine and biology

2023 Volume 1407, Page(s) 85–103

Abstract: Papillomavirus is difficult to culture in vitro, which limits its related research. The development of pseudotyped virus technology provides a valuable research tool for virus infectivity research, vaccine evaluation, infection inhibitor evaluation, and ... ...

Abstract	Papillomavirus is difficult to culture in vitro, which limits its related research. The development of pseudotyped virus technology provides a valuable research tool for virus infectivity research, vaccine evaluation, infection inhibitor evaluation, and so on. Depending on the application fields, different measures have been developed to generate various kinds of pseudotyped papillomavirus. L1-based and L2-based HPV vaccines should be evaluated using different pseudotyped virus system. Pseudotyped papillomavirus animal models need high-titer pseudotyped virus and unique handling procedure to generate robust results. This paper reviewed the development, optimization, standardization, and application of various pseudotyped papillomavirus methods.
MeSH term(s)	Animals ; Capsid Proteins/genetics ; Oncogene Proteins, Viral/genetics ; Viral Pseudotyping ; Papillomavirus Vaccines/genetics ; Papillomavirus Vaccines/therapeutic use ; Papillomavirus Infections/prevention & control ; Antibodies, Viral ; Papillomaviridae/genetics
Chemical Substances	Capsid Proteins ; Oncogene Proteins, Viral ; Papillomavirus Vaccines ; Antibodies, Viral
Language	English
Publishing date	2023-03-15
Publishing country	United States
Document type	Journal Article
ISSN	2214-8019 ; 0065-2598
ISSN (online)	2214-8019
ISSN	0065-2598
DOI	10.1007/978-981-99-0113-5_5
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Pseudotyped Viruses for Coronaviruses.

Wang, Meiyu / Nie, Jianhui / Wang, Youchun

Advances in experimental medicine and biology

2023 Volume 1407, Page(s) 133–151

Abstract: Seven coronaviruses have been identified that can infect humans, four of which usually cause mild symptoms, including HCoV-229E, HCoV-NL63, HCoV-OC43, and HCoV-HKU1, three of which are lethal coronaviruses, named severe acute respiratory syndrome ... ...

Abstract	Seven coronaviruses have been identified that can infect humans, four of which usually cause mild symptoms, including HCoV-229E, HCoV-NL63, HCoV-OC43, and HCoV-HKU1, three of which are lethal coronaviruses, named severe acute respiratory syndrome coronavirus, Middle East respiratory syndrome coronavirus, and severe acute respiratory syndrome coronavirus 2. Pseudotyped virus is an important tool in the field of human coronavirus research because it is safe, easy to prepare, easy to detect, and highly modifiable. In addition to the application of pseudotyped viruses in the study of virus infection mechanism, vaccine, and candidate antiviral drug or antibody evaluation and screening, pseudotyped viruses can also be used as an important platform for further application in the prediction of immunogenicity and antigenicity after virus mutation, cross-species transmission prediction, screening, and preparation of vaccine strains with better broad spectrum and antigenicity. Meanwhile, as clinical trials of various types of vaccines and post-clinical studies are also being carried out one after another, the establishment of a high-throughput and fully automated detection platform based on SARS-CoV-2 pseudotyped virus to further reduce the cost of detection and manual intervention and improve the efficiency of large-scale detection is also a demand for the development of SARS-CoV-2 pseudotyped virus.
MeSH term(s)	Humans ; Viral Pseudotyping ; COVID-19 ; SARS-CoV-2/genetics ; Middle East Respiratory Syndrome Coronavirus/genetics ; Coronavirus 229E, Human/genetics
Language	English
Publishing date	2023-03-15
Publishing country	United States
Document type	Journal Article
ISSN	2214-8019 ; 0065-2598
ISSN (online)	2214-8019
ISSN	0065-2598
DOI	10.1007/978-981-99-0113-5_7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Raw data for Supplementary methods and Figure 3 and 4

Jianhui Nie (8975930)

2020

Abstract: Pseudotyped viruses are useful virological tools due to their safety and versatility. Based on a VSV pseudotyped virus production system, we developed a pseudotyped virus-based neutralization assay against SARS-CoV-2 in biosafety level 2 facilities. ... ...

Abstract	Pseudotyped viruses are useful virological tools due to their safety and versatility. Based on a VSV pseudotyped virus production system, we developed a pseudotyped virus-based neutralization assay against SARS-CoV-2 in biosafety level 2 facilities. Compared with the binding antibody test, the neutralization assay could discriminate the protective agents from the antibody family. This protocol includes production, titration of the SARS-CoV-2 S pseudotyped virus and neutralization assay based on it. Various types of samples targeting virus attachment and entry could be evaluated for their potency, including serum samples derived from animals and humans, monoclonal antibodies, fusion inhibitors (peptides or small molecules). If the pseudotyped virus stock has been prepared in advance, it will take 2 days to get the potency data for the candidate samples. Experience of handling cells is needed before implementing this protocol.
Keywords	Virology ; COVID-19 ; SARS-CoV-2 ; neutralization ; pseudotyped virus ; covid19
Publishing date	2020-06-16T01:18:42Z
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3.

Lu, Qiong / Wu, Hao / Meng, Jing / Wang, Jiangyuan / Wu, Jiajing / Liu, Shuo / Tong, Jincheng / Nie, Jianhui / Huang, Weijin

Frontiers in microbiology

2024 Volume 15, Page(s) 1372069

Abstract: Introduction: Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking.: Methods: We designed a ...

Abstract	Introduction: Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking. Methods: We designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics. Results: The vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization. Discussion: These findings provide a foundation for future HEV vaccine studies.
Language	English
Publishing date	2024-03-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2024.1372069
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: In-situ one-step preparation of anatase/rutile TiO2(A/R) with oxygen vacancy modification derived from 2D Ti3C2 for enhanced visible-light-driven photodegradation of tetracycline

Shi, Jianhui / Yang, Tiantian / Nie, Jianhui / Wang, Hui / Ju, Chenke / Pu, Kaikai / Shi, Jiating / Zhao, Ting

Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2023 June, v. 666 p.131261-

2023

Abstract: The accumulation of residual tetracycline in water environment is an environmental problem that needs to be solved urgently. As an attractive photocatalyst for antibiotic removal, TiO₂ has limited photocatalytic activity due to its low solar light ... ...

Abstract	The accumulation of residual tetracycline in water environment is an environmental problem that needs to be solved urgently. As an attractive photocatalyst for antibiotic removal, TiO₂ has limited photocatalytic activity due to its low solar light utilization efficiency and low quantum yield. Herein, the oxygen vacancy modified Ti₃C₂/TiO₂(anatase/rutile) (TTO₍A/R₎) heterojunction photocatalyst was successfully synthesized and regulated by adjusting the hydrothermal oxidation time using Ti₃C₂ as the metal material and homologous titanium. The TTO₍A/R₎8 h heterojunction photocatalyst with optimal composition percentage of (Ti₃C₂) 30.93%, (A-TiO₂) 54.58% and (R-TiO₂) 14.49% showed the best photocatalytic degradation efficiency of TC, which was increased by 1.5 times compared with commercial P25. Further characterization results indicated that the local states formed by the introduced oxygen vacancy broadened the visible range of TTO₍A/R₎8 h to ∼700 nm. The separation of photogenerated electrons-holes was highly promoted by biphasic TiO₂ and the interface Schottky junction between TiO₂ and Ti₃C₂, which would provide more photogenerated electrons and holes to participate in the photocatalytic reaction. Our research provided a new idea for constructing composite photocatalyst derived from Ti₃C₂ to treat refractory organic pollutants.
Keywords	oxidation ; oxygen ; photocatalysis ; photocatalysts ; photolysis ; solar radiation ; tetracycline ; titanium ; titanium dioxide ; Ti3C2 ; Anatase/rutile TiO2 ; Oxygen vacancy ; Schottky junction ; Tetracycline degradation
Language	English
Dates of publication	2023-06
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	1500517-3
ISSN	0927-7757
ISSN	0927-7757
DOI	10.1016/j.colsurfa.2023.131261
Database	NAL-Catalogue (AGRICOLA)

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Article: Author Correction: A broader neutralizing antibody against all the current VOCs and VOIs targets unique epitope of SARS-CoV-2 RBD.

Liu, Shuo / Jia, Zijing / Nie, Jianhui / Liang, Ziteng / Xie, Jingshu / Wang, Lei / Zhang, Li / Wang, Xiangxi / Wang, Youchun / Huang, Weijin

Cell discovery

2023 Volume 9, Issue 1, Page(s) 122

Language	English
Publishing date	2023-12-08
Publishing country	England
Document type	Published Erratum
ISSN	2056-5968
ISSN	2056-5968
DOI	10.1038/s41421-023-00619-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The complete chloroplast genome of

Li, Jianhui / Yan, Jiawen / Yu, Lin / Bai, Wenfu / Nie, Dongling / Xiong, Ying / Wu, Sizheng

Mitochondrial DNA. Part B, Resources

2022 Volume 7, Issue 1, Page(s) 164–166

Abstract: ... Prunus ... ...

Abstract	Prunus clarofolia
Language	English
Publishing date	2022-01-05
Publishing country	England
Document type	Journal Article
ISSN	2380-2359
ISSN (online)	2380-2359
DOI	10.1080/23802359.2021.2016080
Database	MEDical Literature Analysis and Retrieval System OnLINE

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