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Article ; Online: Dipeptide coacervates as artificial membraneless organelles for bioorthogonal catalysis.

Cao, Shoupeng / Ivanov, Tsvetomir / Heuer, Julian / Ferguson, Calum T J / Landfester, Katharina / Caire da Silva, Lucas

2024 Volume 15, Issue 1, Page(s) 39

Abstract: Artificial organelles can manipulate cellular functions and introduce non-biological processes into cells. Coacervate droplets have emerged as a close analog of membraneless cellular organelles. Their biomimetic properties, such as molecular crowding and ...

Abstract	Artificial organelles can manipulate cellular functions and introduce non-biological processes into cells. Coacervate droplets have emerged as a close analog of membraneless cellular organelles. Their biomimetic properties, such as molecular crowding and selective partitioning, make them promising components for designing cell-like materials. However, their use as artificial organelles has been limited by their complex molecular structure, limited control over internal microenvironment properties, and inherent colloidal instability. Here we report the design of dipeptide coacervates that exhibit enhanced stability, biocompatibility, and a hydrophobic microenvironment. The hydrophobic character facilitates the encapsulation of hydrophobic species, including transition metal-based catalysts, enhancing their efficiency in aqueous environments. Dipeptide coacervates carrying a metal-based catalyst are incorporated as active artificial organelles in cells and trigger an internal non-biological chemical reaction. The development of coacervates with a hydrophobic microenvironment opens an alternative avenue in the field of biomimetic materials with applications in catalysis and synthetic biology.
MeSH term(s)	Dipeptides ; Artificial Cells/chemistry ; Biomolecular Condensates ; Transition Elements/chemistry ; Catalysis ; Organelles/chemistry
Chemical Substances	Dipeptides ; Transition Elements
Language	English
Publishing date	2024-01-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-44278-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Dipeptide coacervates as artificial membraneless organelles for bioorthogonal catalysis

Shoupeng Cao / Tsvetomir Ivanov / Julian Heuer / Calum T. J. Ferguson / Katharina Landfester / Lucas Caire da Silva

Nature Communications, Vol 15, Iss 1, Pp 1-

2024 Volume 14

Abstract: Abstract Artificial organelles can manipulate cellular functions and introduce non-biological processes into cells. Coacervate droplets have emerged as a close analog of membraneless cellular organelles. Their biomimetic properties, such as molecular ... ...

Abstract	Abstract Artificial organelles can manipulate cellular functions and introduce non-biological processes into cells. Coacervate droplets have emerged as a close analog of membraneless cellular organelles. Their biomimetic properties, such as molecular crowding and selective partitioning, make them promising components for designing cell-like materials. However, their use as artificial organelles has been limited by their complex molecular structure, limited control over internal microenvironment properties, and inherent colloidal instability. Here we report the design of dipeptide coacervates that exhibit enhanced stability, biocompatibility, and a hydrophobic microenvironment. The hydrophobic character facilitates the encapsulation of hydrophobic species, including transition metal-based catalysts, enhancing their efficiency in aqueous environments. Dipeptide coacervates carrying a metal-based catalyst are incorporated as active artificial organelles in cells and trigger an internal non-biological chemical reaction. The development of coacervates with a hydrophobic microenvironment opens an alternative avenue in the field of biomimetic materials with applications in catalysis and synthetic biology.
Keywords	Science ; Q
Subject code	540
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The

Nikolova, Galina / Ananiev, Julian / Ivanov, Veselin / Petkova-Parlapanska, Kamelia / Georgieva, Ekaterina / Karamalakova, Yanka

Antioxidants (Basel, Switzerland)

2022 Volume 11, Issue 9

Abstract: Liver damage severity depends on both the dose and the exposure duration. Oxidative stress may increase the Ochratoxine-A (OTA) hepatotoxicity and many antioxidants may counteract toxic liver function. The present study aims to investigate the ... ...

Abstract	Liver damage severity depends on both the dose and the exposure duration. Oxidative stress may increase the Ochratoxine-A (OTA) hepatotoxicity and many antioxidants may counteract toxic liver function. The present study aims to investigate the hepatoprotective potential of
Language	English
Publishing date	2022-08-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox11091678
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Article: Vitamin E and Silymarin Reduce Oxidative Tissue Damage during Gentamycin-Induced Nephrotoxicity.

Georgiev, Tsvetelin / Nikolova, Galina / Dyakova, Viktoriya / Karamalakova, Yanka / Georgieva, Ekaterina / Ananiev, Julian / Ivanov, Veselin / Hadzhibozheva, Petya

Pharmaceuticals (Basel, Switzerland)

2023 Volume 16, Issue 10

Abstract: Aminoglycoside antibiotics and gentamicin (GN), in particular, are still widely used in clinical practice. It is a well-known fact that GN causes nephrotoxicity, and redox disturbances are discussed as a factor in its side effects. Recently, a new type ... ...

Abstract	Aminoglycoside antibiotics and gentamicin (GN), in particular, are still widely used in clinical practice. It is a well-known fact that GN causes nephrotoxicity, and redox disturbances are discussed as a factor in its side effects. Recently, a new type of cell oxidative death, named ferroptosis, was discovered; it is associated with iron accumulation in the cell, glutathione (GSH) depletion and inactivation of glutathione peroxidase-4 (GPX4), reactive oxygen species (ROS) increment with concomitant lipid peroxidation. In this regard, a possible connection between GN-induced renal damage, ferroptosis and the overall antioxidant status of the organism could be investigated. Moreover, due to its beneficial effects, GN is still one of the main choices as a therapeutic agent for several diseases, and the possible reduction of its side effects with the application of certain antioxidants will be of important clinical significance. The study was conducted with adult male white mice divided into several groups (n = 6). GN nephrotoxicity was induced by the administration of GN 100-200 mg/kg i.p. for 10 days. The control group received only saline. The other groups received either Vitamin E (400 mg/kg p.o.) or Silymarin (200 mg/kg p.o.) applied alone or together with GN for the same period. After the end of the study, the animals were sacrificed, and blood and tissue samples were taken for the assessment of biochemical parameters and antioxidant status, as well as routine and specific for GPX4 histochemistry examination. The experimental results indicate that GN-induced nephrotoxicity negatively modulates GPX4 activity and is associated with increased production of ROS and lipid peroxidation. The groups treated with antioxidants demonstrated preserved antioxidant status and better GPX4 activity. In conclusion, the inhibition of ROS production and especially the suppression of ferroptosis, could be of clinical potential and can be applied as a means of reducing the toxic effects of GN application.
Language	English
Publishing date	2023-09-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph16101365
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Conference proceedings ; Online: Characterization of different thawing mechanisms of fibroblast cell-containing tissue models by Mueller polarimetry and statistical analysis

Ivanov, Deyan / Hoeppel, Anika / Schwebler, Julian / Lotz, Christian / Dembski, Sofia / Ossikovski, Razvigor / Novikova, Tatiana

2023

Abstract: Long-term storage and cryopreservation of biological tissues is a global challenge in the fields of regenerative medicine, tissue engineering and implants, because of the formation of large ice crystals that may damage cellular membranes and ... ...

Abstract	Long-term storage and cryopreservation of biological tissues is a global challenge in the fields of regenerative medicine, tissue engineering and implants, because of the formation of large ice crystals that may damage cellular membranes and extracellular matrix (ECM) of collagen. In our studies different thawing mechanisms were tested to achieve more uniform warming of 3D in vitro tissue models, prepared from magnetic nanoparticles (NPs)-modified 3D electrospun nanofiber fleeces and fibroblasts. 3D tissue models frozen in liquid nitrogen were defrosted either with water bath or with radio-frequency (RF) inductive heating of the magnetic NPs and their morphology was compared to that of a non-frozen tissue model using a transmission Mueller microscope and thin sections of all three types of tissue models. Our results demonstrate a sensitivity of the polarimetric parameters obtained with the differential decomposition of Mueller matrices to small changes in sample morphology caused by the different thawing methods. A detailed statistical analysis proved the statistical significance of the experimental data from the three groups of all tissue models.
Keywords	cryopreservation ; fibroblast cells ; Mueller polarimetry ; statistical analysis ; DDC::500 Naturwissenschaften und Mathematik::570 Biowissenschaften ; Biologie::572 Biochemie ; DDC::500 Naturwissenschaften und Mathematik::540 Chemie
Subject code	571
Language	English
Publishing country	de
Document type	Conference proceedings ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The Azadirachta indica (Neem) Seed Oil Reduced Chronic Redox-Homeostasis Imbalance in a Mice Experimental Model on Ochratoxine A-Induced Hepatotoxicity

Nikolova, Galina / Ananiev, Julian / Ivanov, Veselin / Petkova-Parlapanska, Kamelia / Georgieva, Ekaterina / Karamalakova, Yanka

Antioxidants. 2022 Aug. 28, v. 11, no. 9

2022

Abstract	Liver damage severity depends on both the dose and the exposure duration. Oxidative stress may increase the Ochratoxine-A (OTA) hepatotoxicity and many antioxidants may counteract toxic liver function. The present study aims to investigate the hepatoprotective potential of Azadirachta indicaA (A. indica; neem oil) seed oil to reduce acute oxidative disorders and residual OTA toxicity in a 28-day experimental model. The activity of antioxidant and hepatic enzymes, cytokines and the levels of oxidative stress biomarkers –MDA, GSPx, Hydroxiproline, GST, PCC, AGEs, PGC-1, and STIR-1 were analyzed by ELISA. The free radicals ROS and RNS levels were measured by EPR. The protective effects were studied in BALB/C mice treated with A. indica seed oil (170 mg/kg), alone and in combination with OTA (1.25 mg/kg), by gavage daily for 28 days. At the end of the experiment, mice treated with OTA showed changes in liver and antioxidant enzymes, and oxidative stress parameters in the liver and blood. A. indica oil significantly reduced oxidative stress and lipid peroxidation compared to the OTA group. In addition, the hepatic histological evaluation showed significant adipose tissue accumulation in OTA-treated tissues, while treatment with 170 mg/kg A. indica oil showed moderate adipose tissue accumulation.
Keywords	Azadirachta indica ; adipose tissue ; antioxidants ; biomarkers ; blood ; cytokines ; exposure duration ; hepatotoxicity ; histology ; lipid peroxidation ; liver ; liver function ; models ; neem oil ; oxidative stress
Language	English
Dates of publication	2022-0828
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox11091678
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: A pilot study reveals the potential of miR-31-3p and miR-196a-5p as non-invasive biomarkers in advanced laryngeal cancer.

Kyurkchiyan, Silva Garo / Popov, Todor Miroslavov / Shakola, Felitsiya / Rangachev, Julian / Mitev, Vanyo Ivanov / Kaneva, Radka

Folia medica

2021 Volume 63, Issue 3, Page(s) 355–364

Abstract: Introduction: Recently, miRNAs have become popular molecules used as non-invasive biomarkers in cancer diseases.: Aim: The aim of the study was to explore the expression of four miRNAs isoforms: miR-31-3p, miR-196a-5p, miR-210-3p and miR-424-5p in ... ...

Abstract	Introduction: Recently, miRNAs have become popular molecules used as non-invasive biomarkers in cancer diseases. Aim: The aim of the study was to explore the expression of four miRNAs isoforms: miR-31-3p, miR-196a-5p, miR-210-3p and miR-424-5p in plasma and tissue samples from patients with advanced laryngeal squamous cell carcinoma (LSCC) and healthy controls. Materials and methods: Fresh-frozen tumour and normal laryngeal tissue as well as plasma samples were obtained from 22 patients diagnosed with advanced LSCC. The control group included plasma samples from 21 cancer-free volunteers. Total RNA (including miRNAs) extraction, reverse transcription and real time qPCR were the laboratory techniques used in the study. The obtained results were analyzed using SPSS software v. 23. Results: We found that miR-31-3p, miR-196a-5p, and miR-210-3p levels were significantly elevated in laryngeal tumour tissue, but only the levels of miR-31-3p and miR-196a-5p were significantly upregulated in the plasma LSCC target group. Positive correlation was obtained for miR-31-3p (rs=0.443, p=0.039) and miR-196a-5p (rs=0.548; p=0.008) between plasma and adjacent tumour tissue LSCC samples. ROC analyses were used to evaluate the discriminative power of both miRNAs alone and in combination. The combination of miR-31-3p and miR-196a-5p showed best results with AUC=0.978 (95% CI: 0.945-1.000, p.
MeSH term(s)	Biomarkers, Tumor/genetics ; Humans ; Laryngeal Neoplasms/genetics ; MicroRNAs/genetics ; MicroRNAs/supply & distribution ; Pilot Projects ; Squamous Cell Carcinoma of Head and Neck
Chemical Substances	Biomarkers, Tumor ; MIRN31 microRNA, human ; MicroRNAs
Language	English
Publishing date	2021-07-01
Publishing country	Bulgaria
Document type	Journal Article
ZDB-ID	300275-5
ISSN	1314-2143 ; 0430-8638 ; 0204-8043
ISSN (online)	1314-2143
ISSN	0430-8638 ; 0204-8043
DOI	10.3897/folmed.63.e55346
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Zs.A 972: Show issues

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Article ; Online: Targeted suppression of Dpt-specific B cells in humanized Rag2- γc- mouse model of HDM allergy.

Ralchev, Nikola Ralchev / Kerekov, Nikola / Mihaylova, Nikolina / Kremlitzka, Mariann / Hristova, Diana / Dzhorev, Julian / Erdei, Anna / Tchorbanov, Andrey Ivanov

Scandinavian journal of immunology

2022 Volume 97, Issue 2, Page(s) e13241

Abstract: Der p 1 is one of the major allergenic molecules of Dermatophagoides pteronyssinus, causing house dust mite (HDM) allergy. The pathological B cells produce allergen-specific IgE antibodies that mediate the hypersensitivity reaction, therefore the ... ...

Abstract	Der p 1 is one of the major allergenic molecules of Dermatophagoides pteronyssinus, causing house dust mite (HDM) allergy. The pathological B cells produce allergen-specific IgE antibodies that mediate the hypersensitivity reaction, therefore the selective elimination of these B cells is a legitimate therapeutic goal in allergy. Chimeric molecule Dp51-72 able to cross-link B cell inhibitory complement receptor type 1 and BCR on Der p 1-specific B cells was constructed. The signalling capabilities of this molecule have been tested on human B cells. A humanized mouse model of HDM allergy has been used to test the in vivo effects of the chimeric molecule administration. Administering the chimeric molecule to immunodeficient Rag2- γc- mice transferred with PBMCs from allergic patients resulted in reduction of allergen-specific IgE antibodies in the sera, and reduced infiltration of immune cells in lung histology preparations. Reduced numbers of human CD45
MeSH term(s)	Animals ; Humans ; Mice ; Allergens ; Antigens, Dermatophagoides ; Disease Models, Animal ; DNA-Binding Proteins ; Dust Mite Allergy/metabolism ; Hypersensitivity ; Immunoglobulin E ; Nuclear Proteins ; B-Lymphocytes/drug effects ; Receptors, Antigen, B-Cell/drug effects
Chemical Substances	Allergens ; Antigens, Dermatophagoides ; DNA-Binding Proteins ; Immunoglobulin E (37341-29-0) ; Nuclear Proteins ; RAG2 protein, human ; Rag2 protein, mouse ; Receptors, Antigen, B-Cell
Language	English
Publishing date	2022-12-24
Publishing country	England
Document type	Journal Article
ZDB-ID	120476-2
ISSN	1365-3083 ; 0300-9475
ISSN (online)	1365-3083
ISSN	0300-9475
DOI	10.1111/sji.13241
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Zs.A 806: Show issues

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Article: Molecular symmetry perception.

Ivanov, Julian

Journal of chemical information and computer sciences

2004 Volume 44, Issue 2, Page(s) 596–600

Abstract: An algorithm for molecular symmetry perception is presented. The method identifies the full set of molecular symmetry elements (proper and improper) and determines their coordinates. The algorithm eliminates the necessity to explore the entire graph ... ...

Abstract	An algorithm for molecular symmetry perception is presented. The method identifies the full set of molecular symmetry elements (proper and improper) and determines their coordinates. The algorithm eliminates the necessity to explore the entire graph automorphism group; as a result its computer application is extremely effective. Application to several dendrimers and fullerenes with high topological symmetry is presented.
Language	English
Publishing date	2004-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	190019-5
ISSN	0095-2338
ISSN	0095-2338
DOI	10.1021/ci0341868
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Zs.A 1230: Show issues

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Article ; Online: A pilot study reveals the potential of miR-31-3p and miR-196a-5p as non-invasive biomarkers in advanced laryngeal cancer

Silva Garo Kyurkchiyan / Todor Miroslavov Popov / Felitsiya Shakola / Julian Rangachev / Vanyo Ivanov Mitev / Radka Kaneva

Folia Medica, Vol 63, Iss 3, Pp 355-

2021 Volume 364

Abstract: Introduction: Recently, miRNAs have become popular molecules used as non-invasive biomarkers in cancer diseases.Aim: The aim of the study was to explore the expression of four miRNAs isoforms: miR-31-3p, miR-196a-5p, miR-210-3p and miR-424-5p in plasma ... ...

Abstract	Introduction: Recently, miRNAs have become popular molecules used as non-invasive biomarkers in cancer diseases.Aim: The aim of the study was to explore the expression of four miRNAs isoforms: miR-31-3p, miR-196a-5p, miR-210-3p and miR-424-5p in plasma and tissue samples from patients with advanced laryngeal squamous cell carcinoma (LSCC) and healthy controls.Materials and methods: Fresh-frozen tumour and normal laryngeal tissue as well as plasma samples were obtained from 22 patients diagnosed with advanced LSCC. The control group included plasma samples from 21 cancer-free volunteers. Total RNA (including miRNAs) extraction, reverse transcription and real time qPCR were the laboratory techniques used in the study. The obtained results were analyzed using SPSS software v. 23.Results: We found that miR-31-3p, miR-196a-5p, and miR-210-3p levels were significantly elevated in laryngeal tumour tissue, but only the levels of miR-31-3p and miR-196a-5p were significantly upregulated in the plasma LSCC target group. Positive correlation was obtained for miR-31-3p (rs=0.443, p=0.039) and miR-196a-5p (rs=0.548; p=0.008) between plasma and adjacent tumour tissue LSCC samples. ROC analyses were used to evaluate the discriminative power of both miRNAs alone and in combination. The combination of miR-31-3p and miR-196a-5p showed best results with AUC=0.978 (95% CI: 0.945–1.000, p<0.001) with 100% sensitivity and 81% specificity at cut-off: RQ=2.99.Conclusions: Based on this miR-31-3p and miR-196a-5p are proposed as potential biomarkers for validation in larger LSCC group and could be included in a non-invasive miRNAs set for detection of advanced LSCC.
Keywords	laryngeal squamous cell carcinoma ; LSCC ; miRNAs ; n ; Medicine ; R
Subject code	500
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	Pensoft Publishers
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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