LIVIVO - Search results -

Search results

Result 1 - 10 of total 20

Search options

Article: Cervical Intraepithelial Neoplasia Grade 3 in the Third Trimester of Pregnancy: A Case Report.

Agrawal, Laksh S / Mohammad, Shazia / Acharya, Neema / Huse, Shreyash

2023 Volume 15, Issue 10, Page(s) e46703

Abstract: The incidence of malignancies during pregnancy has been on the rise in the recent years, primarily due to an increase in older age pregnancies. This poses a significant risk to both the mother and the developing fetus. We present the case of a 29-year- ... ...

Abstract	The incidence of malignancies during pregnancy has been on the rise in the recent years, primarily due to an increase in older age pregnancies. This poses a significant risk to both the mother and the developing fetus. We present the case of a 29-year-old woman who experienced intermittent vaginal bleeding during her pregnancy. In the last trimester, the patient presented with abnormal vaginal bleeding and abdominal pain. The gestational age was 37.6 weeks. Notably, to our knowledge,
Language	English
Publishing date	2023-10-09
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.46703
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Novel Advances in the Role of Selective Estrogen Receptor Modulators in Hormonal Replacement Therapy: A Paradigm Shift.

Motlani, Gunjan / Motlani, Vidhi / Acharya, Neema / Dave, Apoorva / Pamnani, Soumya / Somyani, Drishti / Agrawal, Shruti

Cureus

2023 Volume 15, Issue 11, Page(s) e49079

Abstract: Estrogen is a key regulatory hormone in the functioning of a female reproductive system. Estrogen hormone regulates many complex physiological processes, which has its role in reproduction and skeletal and cardiovascular systems by acting on estrogen ... ...

Abstract	Estrogen is a key regulatory hormone in the functioning of a female reproductive system. Estrogen hormone regulates many complex physiological processes, which has its role in reproduction and skeletal and cardiovascular systems by acting on estrogen receptors alpha (ERα) and beta (ERβ), which are nuclear transcription factors. Selective estrogen receptor modulators (SERMs) are now being used to treat bone loss, breast carcinoma, and menopausal symptoms, metabolic neurodegenerative because of their characteristics that allow them to function as both estrogen agonists and antagonists, depending on the target tissue. First-generation SERMs, such as Tamoxifen, are used in the management protocol for breast cancer, which is estrogen receptor (ER-positive). Raloxifene is a second-generation SERM that is a valuable adjunct used to treat and prevent osteoporosis in postmenopausal women and prevent compression fractures of the vertebral column. Novel SERM molecules are on the horizon, proven more potent and efficacious in preventing and treating osteoporosis. These include Ospemifene, lasofoxifene, bazedoxifene and arzoxifene. The benefits of Raloxifene versus that of Bazedoxifene are under trial. Despite their therapeutic benefits and actions, these medications are not without adverse effects, such as thromboembolic disorders. Increased risk of uterine cancer has been linked to Tamoxifen. This article delves into the world of SERMs, including their development and discovery. The newer SERMs in late development, ospemifene, lasofoxifene, bazedoxifene, and arzoxifene, are described in detail.
Language	English
Publishing date	2023-11-19
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.49079
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: A Systematic Review of Vertigo: Negligence in Pregnancy.

Toshniwal, Vaishnavi / Agrawal, Aman / Toshniwal, Tejas / Toshniwal, Saket / Khanke, Sankalp / Bakshi, Sanket / Acharya, Neema

Cureus

2022 Volume 14, Issue 10, Page(s) e29814

Abstract: From conception to childbirth, there are many physical, hormonal, and psychological changes that a woman undergoes during pregnancy. During this time, balance is also affected, resulting in symptoms like vertigo and unsteadiness. These symptoms can lead ... ...

Abstract	From conception to childbirth, there are many physical, hormonal, and psychological changes that a woman undergoes during pregnancy. During this time, balance is also affected, resulting in symptoms like vertigo and unsteadiness. These symptoms can lead to physical impairment and disability and can develop at any time. Vertigo in pregnancy has not been extensively written about. The subject of a narrative review is vertigo in pregnant patients. In pregnant women, hormonal alterations in the peripheral tissues and inner ear organs may contribute to vertigo. Meniere's disease, mild convulsive positional dizziness, and oculomotor migraines are all commonly exacerbated by pregnancy. Between the second and third trimesters of pregnancy, specific modifications to proprioception and hearing are also detected during physical examination. Patients who are pregnant typically experience these symptoms throughout this time. Some vertigo conditions can worsen during pregnancy, while others can appear at any time. Understanding audio-vestibular symptoms' pathological and clinical relationship during pregnancy requires more study.
Language	English
Publishing date	2022-10-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.29814
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Lantern on Dome of St. Paul's Cathedral - An Apt Metaphor for a Challenging Leiomyoma.

Acharya, Neema / Singhal, Sonakshi / Agrawal, Mohit / Singh, Nidhi / Verma, Neha

Journal of mid-life health

2020 Volume 11, Issue 3, Page(s) 181–184

Abstract: Fibroids are ubiquitous in women of the reproductive age group more so in midlife. The cervical fibroids are challenging to operating surgeons because it distorts the surgical anatomy of the pelvis and urogenital system. The metaphor given historically ... ...

Abstract	Fibroids are ubiquitous in women of the reproductive age group more so in midlife. The cervical fibroids are challenging to operating surgeons because it distorts the surgical anatomy of the pelvis and urogenital system. The metaphor given historically to this condition is apt as the uterus appears like a lantern sitting on the dome of cervical fibroid similar to the dome of the cathedral. Here, we report two cases of cervical fibroid in perimenopausal age group of different sizes managed by following specific steps.
Language	English
Publishing date	2020-09-29
Publishing country	India
Document type	Case Reports
ZDB-ID	2595412-X
ISSN	0976-7819 ; 0976-7800
ISSN (online)	0976-7819
ISSN	0976-7800
DOI	10.4103/jmh.JMH_143_19
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA.

Agrawal, Neema / Sachdev, Bindiya / Rodrigues, Janneth / Sree, K Sowjanya / Bhatnagar, Raj K

Scientific reports

2013 Volume 3, Page(s) 2292

Abstract: Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase gene expression varies from high to negligible. In the five-day ... ...

Abstract	Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase gene expression varies from high to negligible. In the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. MicroRNA library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several miRNAs. In silico analysis of sequenced miRNAs revealed three miRNAs having sequence similarity to 3'UTR of chitinase. Gene-targeted specific action of these miRNAs, was investigated by luciferase reporter having 3'UTR of chitinase. Only one of three miRNAs, miR-24, inhibited luciferase expression. Further, a day-wise in vivo quantification of miR-24 in fifth-instar larvae revealed a negative correlation with corresponding chitinase transcript abundance. The force-feeding of synthetic miR-24 induced significant morphological aberrations accompanied with arrest of molting. These miR-24 force-fed larvae revealed significantly reduced chitinase transcript abundance.
MeSH term(s)	3' Untranslated Regions ; Animals ; Base Sequence ; Cell Line ; Chitinases/chemistry ; Chitinases/genetics ; Chitinases/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Larva/genetics ; Larva/metabolism ; Metamorphosis, Biological/genetics ; MicroRNAs/genetics ; Molecular Sequence Data ; Moths/enzymology ; Moths/genetics ; Phenotype ; Reproducibility of Results ; Time Factors ; Transcription, Genetic
Chemical Substances	3' Untranslated Regions ; MicroRNAs ; Chitinases (EC 3.2.1.14)
Language	English
Publishing date	2013-07-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/srep02292
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mouse strain variation in SMA(+) myofibroblast development after corneal injury.

Singh, Vivek / Torricelli, Andre A M / Nayeb-Hashemi, Neema / Agrawal, Vandana / Wilson, Steven E

Experimental eye research

2013 Volume 115, Page(s) 27–30

Abstract: The purpose of present study was to investigate differences in myofibroblast development after haze-generating injury in different commonly used strains of mice. The inbred mouse strains used in this study were Balb/c, C57BL/6, C3H/HeJ and DBA/1J. All ... ...

Abstract	The purpose of present study was to investigate differences in myofibroblast development after haze-generating injury in different commonly used strains of mice. The inbred mouse strains used in this study were Balb/c, C57BL/6, C3H/HeJ and DBA/1J. All mice had uniform irregular phototherapeutic keratectomy with an excimer laser according to a previously published method to generate stromal haze. DBA/1J mice generated significantly greater density of alpha smooth muscle actin (SMA)-positive myofibroblasts in the anterior stroma compared to Balb/c (p < 0.05), C57BL/6 (p < 0.05) and C3H/HeJ (p < 0.01) mice. The C3H/HeJ strain had significantly lower density of SMA-positive myofibroblasts compared to other three mouse strains. These results indicate that mouse strain must be considered in designing experiments and interpreting the results of experiments in which corneal haze and myofibroblast generation is studied in mice. Further investigation of genetics underlying mouse strain variation could provide insight into the corneal wound healing and haze generation processes.
MeSH term(s)	Actins/metabolism ; Animals ; Corneal Injuries ; Corneal Opacity/pathology ; Corneal Stroma/pathology ; Eye Injuries/metabolism ; Eye Injuries/pathology ; Female ; Lasers, Excimer/therapeutic use ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Myofibroblasts/metabolism ; Myofibroblasts/pathology ; Photorefractive Keratectomy ; Wound Healing/physiology
Chemical Substances	Acta2 protein, mouse ; Actins
Language	English
Publishing date	2013-06-18
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	80122-7
ISSN	1096-0007 ; 0014-4835
ISSN (online)	1096-0007
ISSN	0014-4835
DOI	10.1016/j.exer.2013.06.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 163: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Purification and characterization of aminopeptidase N from Spodoptera litura expressed in Sf21 insect cells.

Kaur, Ravinder / Agrawal, Neema / Bhatnagar, Raj

Protein expression and purification

2007 Volume 54, Issue 2, Page(s) 267–274

Abstract: Insecticidal crystal proteins produced by strains of Bacillus thuringiensis cause larval death upon interaction with specific receptors located at the midgut epithelium of susceptible insects. Large quantities of easily purified aminopeptidase and ... ...

Abstract	Insecticidal crystal proteins produced by strains of Bacillus thuringiensis cause larval death upon interaction with specific receptors located at the midgut epithelium of susceptible insects. Large quantities of easily purified aminopeptidase and cadherin-like Cry toxin receptors can facilitate the further study of Cry toxin binding and pore formation. Here, we report the solubilisation and purification of aminopeptidase N from Spodoptera litura (SlAPN). Recombinantly expressed and membrane anchored aminopeptidase N showed differential solubilisation with various ionic and nonionic detergents. The N-lauryl sarcosine (NLS)-solubilised SlAPN was purified to near homogeneity by anion exchange and gel filtration chromatography and refolded to its catalytically active form. The optimized purification regimen lead to >90% purification of the catalytically active SlAPN with 11% recovery and 9-folds purification. The interaction of purified SlAPN with biologically active Cry1C protein has been qualitatively and quantitatively characterized. By ligand blotting experiment, we demonstrated the linearity of interaction of the two purified proteins and lack of interaction of SlAPN with structurally divergent nontoxic Cry1Ac protein. The equilibrium dissociation constant (K(D)) of purified SlAPN for Cry1C was calculated by ELISA (90nM). Interaction of enzymatically inactive SlAPN with Cry1C and catalytic activity of APN-Cry1C complex suggested that the catalytic site and toxin-binding sites of SlAPN do not overlap.
MeSH term(s)	Animals ; Bacterial Proteins/metabolism ; Bacterial Toxins/metabolism ; CD13 Antigens/biosynthesis ; CD13 Antigens/isolation & purification ; Endotoxins/metabolism ; Hemolysin Proteins/metabolism ; Protein Folding ; Protein Interaction Mapping ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/isolation & purification ; Solubility ; Spodoptera/enzymology
Chemical Substances	Bacterial Proteins ; Bacterial Toxins ; Endotoxins ; Hemolysin Proteins ; Recombinant Proteins ; insecticidal crystal protein, Bacillus Thuringiensis ; CD13 Antigens (EC 3.4.11.2)
Language	English
Publishing date	2007-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1055455-5
ISSN	1096-0279 ; 1046-5928
ISSN (online)	1096-0279
ISSN	1046-5928
DOI	10.1016/j.pep.2007.03.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3084: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Expression profile of prophenoloxidase-encoding (acppo6) gene of Plasmodium vivax-refractory strain of Anopheles culicifacies.

Sharma, Anil / Rodrigues, Janneth / Kajla, Mayur K / Agrawal, Neema / Adak, T / Bhatnagar, Raj K

Journal of medical entomology

2010 Volume 47, Issue 6, Page(s) 1220–1226

Abstract: Anopheles culicifacies is the main vector for transmission of Plasmodium vivax malaria in the Indian subcontinent. A strain of An. culicifacies isolated from its natural niche displayed complete refractoriness to P. vivax by melanotic encapsulation of ... ...

Abstract	Anopheles culicifacies is the main vector for transmission of Plasmodium vivax malaria in the Indian subcontinent. A strain of An. culicifacies isolated from its natural niche displayed complete refractoriness to P. vivax by melanotic encapsulation of ookinetes. Prophenoloxidases are key components of the phenoloxidase cascade that leads to recognition and melanization of invading organisms. We isolated and cloned prophenoloxidase-encoding acppo6 gene of An. culicifacies and analyzed its expression profile under various regimens of immune challenge. The acppo6 was differentially expressed during various stages of larval development. The acppo6 transcription was also up-regulated in response to bacteria and Plasmodium vinckei petteri challenge. The transcript levels of the acppo6 gene were higher in naive adult refractory female mosquitoes as compared with female susceptible mosquitoes. Furthermore, the induction of acppo6 in the susceptible strain upon Plasmodium infection was negligible as compared with that of the refractory strain. The observation is suggestive of the role of acppo6 in effectuating a melanotic response in Plasmodium-incompetent naturally occurring refractory An. culicifacies strain.
MeSH term(s)	Amino Acid Sequence ; Animals ; Anopheles/enzymology ; Anopheles/genetics ; Anopheles/parasitology ; Catechol Oxidase/genetics ; Catechol Oxidase/metabolism ; Enzyme Precursors/genetics ; Enzyme Precursors/metabolism ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Enzymologic ; Host-Parasite Interactions/genetics ; Molecular Sequence Data ; Plasmodium vivax/physiology
Chemical Substances	Enzyme Precursors ; pro-phenoloxidase (EC 1.10.3.-) ; Catechol Oxidase (EC 1.10.3.1)
Language	English
Publishing date	2010-12-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	410635-0
ISSN	0022-2585
ISSN	0022-2585
DOI	10.1603/me10033
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 303: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: siRNA-directed silencing of transgene expressed in cultured insect cells.

Agrawal, Neema / Malhotra, Pawan / Bhatnagar, Raj K

Biochemical and biophysical research communications

2004 Volume 320, Issue 2, Page(s) 428–434

Abstract: RNA interference (RNAi) has emerged as a powerful tool to rapidly analyze gene functions in a wide variety of eukaryotic organisms as well as in cultured cell lines. We demonstrate here that RNAi can be applied to study the function of a transgene ... ...

Abstract	RNA interference (RNAi) has emerged as a powerful tool to rapidly analyze gene functions in a wide variety of eukaryotic organisms as well as in cultured cell lines. We demonstrate here that RNAi can be applied to study the function of a transgene expressed in an insect cell line (Spodoptera frugiperda, Sf21). The aminopeptidase N gene (apn) targeted for silencing in the present study was isolated from the midgut of Spodoptera litura larvae and expressed in Sf21 cells using baculovirus expression system. The recombinant APN protein expressed at the surface of Sf21 cells was shown to interact with insecticidal crystal protein, Cry1C, by in vitro experiments. The exogenous addition/transfection of APN dsRNA or siRNA in the cultured cells resulted in partial/complete inhibition of expression of apn leading to the loss of toxin binding to the transgene expressing cells. These experiments highlighted the usefulness of RNAi as a tool to study the function of an expressed transgene in insect cell line and to study the specificity of receptor-ligand interaction.
MeSH term(s)	Animals ; Base Sequence ; Blotting, Northern ; Cells, Cultured ; Gene Silencing/physiology ; Ligands ; RNA ; RNA, Small Interfering/physiology ; Spodoptera/cytology ; Spodoptera/genetics ; Transgenes
Chemical Substances	Ligands ; RNA primers ; RNA, Small Interfering ; RNA (63231-63-0)
Language	English
Publishing date	2004-07-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2004.05.184
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 116: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Expression Profile of Prophenoloxidase-Encoding (acppo6) Gene of Plasmodium vivax-Refractory Strain of Anopheles culicifacies

Sharma, Anil / Rodrigues, Janneth / Kajla, Mayur K / Agrawal, Neema / Adak, T / Bhatnagar, Raj K

Journal of medical entomology. 2010 Nov., v. 47, no. 6

2010

Abstract	Anopheles culicifacies is the main vector for transmission of Plasmodium vivax malaria in the Indian subcontinent. A strain of An. culicifacies isolated from its natural niche displayed complete refractoriness to P. vivax by melanotic encapsulation of ookinetes. Prophenoloxidases are key components of the phenoloxidase cascade that leads to recognition and melanization of invading organisms. We isolated and cloned prophenoloxidase-encoding acppo6 gene of An. culicifacies and analyzed its expression profile under various regimens of immune challenge. The acppo6 was differentially expressed during various stages of larval development. The acppo6 transcription was also up-regulated in response to bacteria and Plasmodium vinckei petteri challenge. The transcript levels of the acppo6 gene were higher in naive adult refractory female mosquitoes as compared with female susceptible mosquitoes. Furthermore, the induction of acppo6 in the susceptible strain upon Plasmodium infection was negligible as compared with that of the refractory strain. The observation is suggestive of the role of acppo6 in effectuating a melanotic response in Plasmodium-incompetent naturally occurring refractory An. culicifacies strain.
Keywords	Anopheles culicifacies ; Plasmodium vivax ; prophenoloxidase ; larval development ; isolation ; molecular cloning ; gene expression
Language	English
Dates of publication	2010-11
Size	p. 1220-1226.
Document type	Article
ZDB-ID	410635-0
ISSN	0022-2585
ISSN	0022-2585
DOI	10.1603/ME10033
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 78/543: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED