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  1. Book ; Online ; E-Book: RNA Nanotechnology and Therapeutics

    Guo, Peixuan / Afonin, Kirill A.

    2022  

    Keywords Electronic books
    Subject code 572.8/8
    Language English
    Size 1 online resource (644 pages)
    Edition 2nd ed.
    Publisher Taylor & Francis Group
    Publishing place Milton
    Document type Book ; Online ; E-Book
    Note Description based on publisher supplied metadata and other sources
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT021434023
    ISBN 978-1-00-055273-7 ; 9781138312869 ; 1-00-055273-X ; 113831286X
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book: RNA nanotechnology and therapeutics

    Guo, Peixuan / Haque, Farzin

    methods and protocols

    (Methods in molecular biology ; 1297 ; Springer protocols)

    2015  

    Author's details ed. by Peixuan Guo ; Farzin Haque
    Series title Methods in molecular biology ; 1297
    Springer protocols
    Collection
    Keywords Nanotechnology / methods ; RNA / therapeutic use ; Nanomedicine / methods ; Nanoparticles
    Language English
    Size XI, 239 S. : Ill., graph. Darst.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Includes bibliographical references and index
    HBZ-ID HT018641281
    ISBN 978-1-4939-2562-9 ; 1-4939-2562-8
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1297- verfügbar in Königswinter Königswinter

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  3. Article ; Online: High resolution structure of hexameric herpesvirus DNA-packaging motor elucidates revolving mechanism and ends 20-year fervent debate.

    Guo, Peixuan

    Protein & cell

    2020  Volume 11, Issue 5, Page(s) 311–315

    MeSH term(s) DNA ; DNA Packaging
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2020-04-20
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Comment
    ZDB-ID 2543451-2
    ISSN 1674-8018 ; 1674-800X
    ISSN (online) 1674-8018
    ISSN 1674-800X
    DOI 10.1007/s13238-020-00714-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Optical tweezer and TIRF microscopy for single molecule manipulation of RNA/DNA nanostructures including their rubbery property and single molecule counting.

    Ghimire, Chiran / Guo, Peixuan

    Biophysics reports

    2023  Volume 7, Issue 6, Page(s) 449–474

    Abstract: Life science is often focused on the microscopic level. Single-molecule technology has been used to observe components at the micro- or nanoscale. Single-molecule imaging provides unprecedented information about the behavior of individual molecules in ... ...

    Abstract Life science is often focused on the microscopic level. Single-molecule technology has been used to observe components at the micro- or nanoscale. Single-molecule imaging provides unprecedented information about the behavior of individual molecules in contrast to the information from ensemble methods that average the information of many molecules in various states. A typical feature of living systems is motion. The lack of synchronicity of motion biomachines in living systems makes it challenging to image the motion process with high resolution. Thus, single-molecule technology is especially useful for real-time study on motion mechanism of biomachines, such as viral DNA packaging motor, or other ATPases. The most common optical instrumentations in single-molecule studies are optical tweezers and single molecule total internal refection fluorescence microscopy (smTIRF). Optical tweezers are the force-based technique. The analysis of RNA using optical tweezer has led to the discovery of the rubbery or amoeba property of RNA nanoparticles for compelling vessel extravasation to enhance tumor targeting and fast renal excretion. The rubbery property of RNA lends mechanistic evidence for RNAs use as an ideal reagent in cancer treatment with undetectable toxicity. Single molecule photobleaching allows for the direct counting of biomolecules. This technique was invented for single molecule counting of RNA in the phi29 DNA packaging motor to resolve the debate between five and six copies of RNA in the motor. The technology has subsequently extended to counting components in biological machines composed of protein, DNA, and other macromolecules. In combination with statistical analysis, it reveals biomolecular mechanisms in detail and leads to the development of ultra-sensitive sensors in diagnosis and forensics. This review focuses on the applications of optical tweezers and fluorescence-based techniques as single-molecule technologies to resolve mechanistic questions related to RNA and DNA nanostructures.
    Language English
    Publishing date 2023-06-06
    Publishing country China
    Document type Journal Article
    ISSN 2364-3420
    ISSN (online) 2364-3420
    DOI 10.52601/bpr.2021.210003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Targeting oncogenic

    Yang, Linlin / Li, Zhefeng / Binzel, Daniel W / Guo, Peixuan / Williams, Terence M

    Molecular therapy. Nucleic acids

    2023  Volume 33, Page(s) 559–571

    Abstract: ... ...

    Abstract KRAS
    Language English
    Publishing date 2023-07-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2662631-7
    ISSN 2162-2531
    ISSN 2162-2531
    DOI 10.1016/j.omtn.2023.07.027
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Revolving ATPase motors as asymmetrical hexamers in translocating lengthy dsDNA via conformational changes and electrostatic interactions in phi29, T7, herpesvirus, mimivirus,

    Weitao, Tao / Grandinetti, Giovanna / Guo, Peixuan

    Exploration (Beijing, China)

    2023  Volume 3, Issue 2, Page(s) 20210056

    Abstract: Investigations of the parallel architectures of biomotors in both prokaryotic and eukaryotic systems suggest a similar revolving mechanism in the use of ATP to drive translocation of the lengthy double-stranded (ds)DNA genomes. This mechanism is ... ...

    Abstract Investigations of the parallel architectures of biomotors in both prokaryotic and eukaryotic systems suggest a similar revolving mechanism in the use of ATP to drive translocation of the lengthy double-stranded (ds)DNA genomes. This mechanism is exemplified by the dsDNA packaging motor of bacteriophage phi29 that operates through revolving but not rotating dsDNA to "Push through a one-way valve". This unique and novel revolving mechanism discovered in phi29 DNA packaging motor was recently reported in other systems including the dsDNA packaging motor of herpesvirus, the dsDNA ejecting motor of bacteriophage T7, the plasmid conjugation machine TraB in
    Language English
    Publishing date 2023-02-05
    Publishing country China
    Document type Journal Article ; Review
    ISSN 2766-2098
    ISSN (online) 2766-2098
    DOI 10.1002/EXP.20210056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: ErZhiTianGui Decoction alleviates age-related ovarian aging by regulating mitochondrial homeostasis and inhibiting ferroptosis.

    Zhicheng, Jia / Yongqian, Li / Peixuan, Wang / Kai, Yang / Mengyu, Shi / Wen, Chen / Qihui, Liang / Ying, Guo

    Journal of ovarian research

    2024  Volume 17, Issue 1, Page(s) 12

    Abstract: Aim: This study was designed to investigate the pharmacological effects and mechanisms of ErZhiTianGui Decoction (EZTG) for age-related ovarian aging in mice.: Methods: This study used naturally aging mice as a model, and EZTG was used for ... ...

    Abstract Aim: This study was designed to investigate the pharmacological effects and mechanisms of ErZhiTianGui Decoction (EZTG) for age-related ovarian aging in mice.
    Methods: This study used naturally aging mice as a model, and EZTG was used for intragastric administration. Ovarian pathological changes, as well as follicular reserve were assessed by hematoxylin and eosin staining, and serum hormone levels (anti-mullerian hormone, follicle-stimulating hormone), mitochondrial reactive oxygen species (ROS) and mitochondrial DNA (mtDNA) damage marker 8-hydroxy-2'-deoxyguanosine(8-OHdG), and lipid peroxidation markers glutathione(GSH) and malondialdehyde(MDA) were determined by enzyme-linked immunosorbent assay. Mitochondrial membrane potential (MMP) levels in ovaries were determined using flow cytometry. The levels of PINK1 and Parkin were observed using immunofluorescence staining. Mitochondrial-derived vesicles (MDVs) and mitochondrial morphology were observed using electron microscopy. Prussian blue staining was used to observe iron ion aggregation in ovarian tissue. The Iron assay kits detected total iron levels. Western blot was used to detect the expression of proteins related to mitochondrial and ferroptosis related genes.
    Results: After EZTG treatment, aged mice showed increased ovarian reserve, improved serum hormone levels, increased MMP, GSH levels, and decreased mitochondrial ROS, 8-OHdG, and MDA levels. Immunofluorescence staining showed decreased levels of PINK1 and Parkin, and the same trend was observed for the Western blot. Meanwhile, electron microscopy showed that EZTG improved the mitochondrial morphology in the ovaries of aged mice. EZTG also decreased the total iron and protein levels of Acyl-CoA synthetase long-chain family4 (ACSL4) and increased the protein level of glutathione peroxidase 4 (GPX4) in the ovaries of aged mice.
    Conclusions: EZTG can maintain PINK1/Parkin-mediated mitochondrial homeostasis, reduce the lipid peroxidation caused by the accumulation of ROS, and inhibit the occurrence of ferroptosis and delaying ovarian aging. These findings suggest that EZTG may be a promising drug for treating age-related ovarian aging in females.
    MeSH term(s) Female ; Animals ; Mice ; Ovary ; Ferroptosis ; Reactive Oxygen Species ; 8-Hydroxy-2'-Deoxyguanosine ; Anti-Mullerian Hormone ; Glutathione ; Homeostasis ; Iron ; Ubiquitin-Protein Ligases ; Protein Kinases
    Chemical Substances Reactive Oxygen Species ; 8-Hydroxy-2'-Deoxyguanosine (88847-89-6) ; Anti-Mullerian Hormone (80497-65-0) ; Glutathione (GAN16C9B8O) ; Iron (E1UOL152H7) ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; Protein Kinases (EC 2.7.-)
    Language English
    Publishing date 2024-01-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 2455679-8
    ISSN 1757-2215 ; 1757-2215
    ISSN (online) 1757-2215
    ISSN 1757-2215
    DOI 10.1186/s13048-023-01341-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Radiolabeled RNA Nanoparticles for Highly Specific Targeting and Efficient Tumor Accumulation with Favorable

    Wang, Hongzhi / Guo, Peixuan

    Molecular pharmaceutics

    2021  Volume 18, Issue 8, Page(s) 2924–2934

    Abstract: Therapeutic efficiency and toxicity are two of the three critical factors in molecular therapy and pharmaceutical drug development. Specific tumor targeting and rapid renal excretion contribute to improving efficiency and reducing toxicity. We recently ... ...

    Abstract Therapeutic efficiency and toxicity are two of the three critical factors in molecular therapy and pharmaceutical drug development. Specific tumor targeting and rapid renal excretion contribute to improving efficiency and reducing toxicity. We recently found that RNA nanoparticles display rubber-like properties, enabling them to deliver therapeutics to cancer with high efficiency. Off-target RNA nanoparticles were rapidly cleared by renal excretion, resulting in nontoxicity. However, previous biodistribution studies relied mainly on fluorescent markers, which can cause interference from fluorophore quenching and autofluorescence. Thus, the quantification of biodistribution requires further scrutiny. In this study, radionuclide [
    MeSH term(s) Animals ; Breast Neoplasms/drug therapy ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Drug Stability ; Female ; Humans ; Injections, Intravenous ; Mice ; Nanoparticle Drug Delivery System/chemistry ; Nanoparticles/chemistry ; RNA/administration & dosage ; RNA/pharmacokinetics ; Tissue Distribution ; Tritium/administration & dosage ; Xenograft Model Antitumor Assays
    Chemical Substances Nanoparticle Drug Delivery System ; Tritium (10028-17-8) ; RNA (63231-63-0)
    Language English
    Publishing date 2021-07-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2138405-8
    ISSN 1543-8392 ; 1543-8384
    ISSN (online) 1543-8392
    ISSN 1543-8384
    DOI 10.1021/acs.molpharmaceut.1c00035
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6250: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Identification of Arginine Finger as the Starter of the Biomimetic Motor in Driving Double-Stranded DNA.

    Liang, Chenxi / Guo, Peixuan

    ACS nano

    2021  Volume 15, Issue 8, Page(s) 13260–13266

    Abstract: Nanomotors in nanotechnology may be as important as cars in daily life. Biomotors are nanoscale machines ubiquitous in living systems to carry out ATP-driven activities such as walking, breathing, blinking, mitosis, replication, transcription, and ... ...

    Abstract Nanomotors in nanotechnology may be as important as cars in daily life. Biomotors are nanoscale machines ubiquitous in living systems to carry out ATP-driven activities such as walking, breathing, blinking, mitosis, replication, transcription, and trafficking. The sequential action in an asymmetrical hexamer by a revolving mechanism has been confirmed in dsDNA packaging motors of phi29, herpesviruses, bacterial dsDNA translocase FtsK, and Streptomyces TraB for conjugative dsDNA transfer. These elaborate, delicate, and exquisite ring structures have inspired scientists to design biomimetics in nanotechnology. Many multisubunit ATPase rings generate force via sequential action of multiple modules, such as the Walker A, Walker B, P-loop, arginine finger, sensors, and lid. The chemical to mechanical energy conversion usually takes place in sequential order. It is commonly believed that ATP binding triggers such conversion, but how the multimodule motor starts the sequential process has not been explicitly investigated. Identification of the starter is of great significance for biomimetic motor fabrication. Here, we report that the arginine finger is the starter of the motor. Only one amino acid residue change in the arginine finger led to the impediment and elimination of all following steps. Without the arginine finger, the motor failed to assemble, bind ATP, recruit DNA, or hydrolyze ATP and was eventually unable to package DNA. However, the loss of ATPase activity due to an inactive arginine finger can be rescued by an arginine finger from the adjacent subunit of Walker A mutant through trans-complementation. Taken together, we demonstrate that the formation of dimers triggered by the arginine finger initiates the motor action rather than the general belief of initiation by ATP binding.
    MeSH term(s) DNA Packaging ; Biomimetics ; Arginine ; DNA, Viral ; Adenosine Triphosphatases/chemistry ; Adenosine Triphosphate/metabolism ; Virus Assembly
    Chemical Substances Arginine (94ZLA3W45F) ; DNA, Viral ; Adenosine Triphosphatases (EC 3.6.1.-) ; Adenosine Triphosphate (8L70Q75FXE)
    Language English
    Publishing date 2021-07-29
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1936-086X
    ISSN (online) 1936-086X
    DOI 10.1021/acsnano.1c02973
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Direct medical costs of ischemic heart disease in urban Southern China

    Peixuan Xie / Xuezhu Li / Feifan Guo / Donglan Zhang / Hui Zhang

    Frontiers in Public Health, Vol

    a 5-year retrospective analysis of an all-payer health claims database in Guangzhou City

    2023  Volume 11

    Abstract: IntroductionThis study aimed to estimate the direct medical costs and out-of-pocket (OOP) expenses associated with inpatient and outpatient care for IHD, based on types of health insurance. Additionally, we sought to identify time trends and factors ... ...

    Abstract IntroductionThis study aimed to estimate the direct medical costs and out-of-pocket (OOP) expenses associated with inpatient and outpatient care for IHD, based on types of health insurance. Additionally, we sought to identify time trends and factors associated with these costs using an all-payer health claims database among urban patients with IHD in Guangzhou City, Southern China.MethodsData were collected from the Urban Employee-based Basic Medical Insurance (UEBMI) and the Urban Resident-based Basic Medical Insurance (URBMI) administrative claims databases in Guangzhou City from 2008 to 2012. Direct medical costs were estimated in the entire sample and by types of insurance separately. Extended Estimating Equations models were employed to identify the potential factors associated with the direct medical costs including inpatient and outpatient care and OOP expenses.ResultsThe total sample included 58,357 patients with IHD. The average direct medical costs per patient were Chinese Yuan (CNY) 27,136.4 [US dollar (USD) 4,298.8] in 2012. The treatment and surgery fees were the largest contributor to direct medical costs (52.0%). The average direct medical costs of IHD patients insured by UEBMI were significantly higher than those insured by the URBMI [CNY 27,749.0 (USD 4,395.9) vs. CNY 21,057.7(USD 3,335.9), P < 0.05]. The direct medical costs and OOP expenses for all patients increased from 2008 to 2009, and then decreased during the period of 2009–2012. The time trends of direct medical costs between the UEBMI and URBMI patients were different during the period of 2008-2012. The regression analysis indicated that the UEBMI enrollees had higher direct medical costs (P < 0.001) but had lower OOP expenses (P < 0.001) than the URBMI enrollees. Male patients, patients having percutaneous coronary intervention operation and intensive care unit admission, patients treated in secondary hospitals and tertiary hospitals, patients with the LOS of 15–30 days, 30 days and longer had significantly higher direct ...
    Keywords ischemic heart disease ; direct medical costs ; health insurance ; time trends ; China ; out-of-pocket (OOP) expenses ; Public aspects of medicine ; RA1-1270
    Subject code 610
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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