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Article ; Online: Effects of consecutive workdays and days off on low back pain, fatigue and stress: prospective cohort study among warehouse and construction workers.

Bláfoss, Rúni / Aagaard, Per / Clausen, Thomas / Andersen, Lars L

2023 Volume 80, Issue 11, Page(s) 650–658

Abstract: Objectives: Limited knowledge exists about day-to-day changes in physical and mental symptoms in warehouse and construction workers. This study investigated the associations between consecutive workdays and days off with low back pain (LBP) intensity, ... ...

Abstract	Objectives: Limited knowledge exists about day-to-day changes in physical and mental symptoms in warehouse and construction workers. This study investigated the associations between consecutive workdays and days off with low back pain (LBP) intensity, bodily fatigue and mental stress. Methods: Participants (n=224) received daily questions for 21 days about LBP, fatigue, stress (outcome, 0-10 scales), and workdays and days off (exposure). We tested associations between 1-3 workdays (n=148) and 1-2 days off (n=158) with LBP intensity, bodily fatigue and mental stress after work and the following morning using linear mixed models with repeated measures controlling for relevant confounders. Results: Consecutive workdays led to progressively increased LBP intensity, with three workdays increasing LBP intensity by 1.76 (95% CI 1.48 to 2.03) points. Bodily fatigue and mental stress increased after one workday (2.06 (95% CI 1.80 to 2.32) and 0.97 (95% CI 0.77 to 1.17) points, respectively) and remained stable for three workdays. After 1 day off, bodily fatigue and mental stress decreased -1.82 (95% CI -2.03 to -1.61) and -0.88 (95% CI -1.05 to -0.71) points, respectively, without decreasing further. In contrast, LBP intensity decreased progressively -1.09 (95% CI -1.27 to -0.91) and -1.45 (95% CI -1.67 to -1.24) points after 1 and 2 days off, respectively. Conclusions: Workdays and days off affected the outcome variables differently. LBP intensity progressively increased with consecutive workdays, while workers needed 2 days off to recover. This study provides valuable knowledge about how to organise the workweek to prevent LBP, fatigue and stress, potentially reducing labour market withdrawal.
MeSH term(s)	Humans ; Low Back Pain/epidemiology ; Low Back Pain/etiology ; Construction Industry ; Prospective Studies ; Fatigue/etiology ; Occupations ; Occupational Diseases/epidemiology ; Occupational Diseases/etiology ; Occupational Diseases/diagnosis
Language	English
Publishing date	2023-10-13
Publishing country	England
Document type	Journal Article
ZDB-ID	1180733-7
ISSN	1470-7926 ; 1351-0711
ISSN (online)	1470-7926
ISSN	1351-0711
DOI	10.1136/oemed-2023-109043
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Article ; Online: Association of objectively measured lifting load with low-back pain, stress, and fatigue: A prospective cohort study.

Bláfoss, Rúni / Aagaard, Per / Clausen, Thomas / Andersen, Lars L

Scandinavian journal of work, environment & health

2023 Volume 50, Issue 1, Page(s) 11–21

Abstract: Objectives: Limited knowledge exists about the association of lifting loads on a daily basis with physical and mental symptoms among warehouse workers. This study investigated associations between objectively measured lifting load and low-back pain (LBP) ...

Abstract	Objectives: Limited knowledge exists about the association of lifting loads on a daily basis with physical and mental symptoms among warehouse workers. This study investigated associations between objectively measured lifting load and low-back pain (LBP), mental stress, and bodily fatigue after work and the following morning. Methods: Warehouse workers (N=85) from the retail industry replied to daily questionnaires before and after work for 21 days about LBP intensity, mental stress, and bodily fatigue (outcome, all scales 0-10). We assessed lifting exposure using company records from the warehouse logistic systems on total lifting load (kg) per workday. Associations between variables were tested using linear mixed models with repeated measures controlling for relevant confounders. Results: Mean daily lifting load was 1667.2 kg (range: 0-9998.4 kg). Compared to lifting 0-499 kg during a workday, lifting 500-1999 kg was associated with 0.59 points [95% confidence interval (CI) 0.10-1.08] elevated LBP intensity after work, while lifting ≥5000 showed a higher LBP intensity of 1.26 points (95% CI 0.48-2.03). LBP intensity remained elevated the following morning. Lifting ≥5000 kg was associated with higher mental stress after work of 0.74 points (95% CI 0.10-1.37), while no association was observed for bodily fatigue. Conclusions: Higher daily lifting loads were associated with higher LBP intensity after work and the following morning. These findings suggest that warehouses should consider the daily lifting loads when organizing warehouse work to prevent development of LBP, eg, using company records to provide a more equal distribution of daily lifting loads between workers.
MeSH term(s)	Humans ; Prospective Studies ; Lifting/adverse effects ; Risk Factors ; Low Back Pain/etiology ; Industry ; Occupational Diseases/epidemiology ; Occupational Diseases/etiology
Language	English
Publishing date	2023-11-06
Publishing country	Finland
Document type	Journal Article
ZDB-ID	191563-0
ISSN	1795-990X ; 0355-3140
ISSN (online)	1795-990X
ISSN	0355-3140
DOI	10.5271/sjweh.4127
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Zs.A 1153: Show issues

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Article ; Online: Postchemotherapy Retroperitoneal Lumpectomy in Patients with Nonseminoma Testicular Cancer: A Nationwide Study.

Rosenvilde, Josephine Julie / Lauritsen, Jakob / Bandak, Mikkel / Wagner, Thomas / Agerbæk, Mads / Dysager, Lars / Aagaard, Mikael / Daugaard, Gedske

European urology oncology

2024

Abstract: Background and objective: Optimal treatment outcomes in patients with metastatic nonseminoma testicular cancer are achieved with chemotherapy and subsequent surgery in cases with residual tumor. In Denmark, postchemotherapy retroperitoneal lumpectomy ( ... ...

Abstract	Background and objective: Optimal treatment outcomes in patients with metastatic nonseminoma testicular cancer are achieved with chemotherapy and subsequent surgery in cases with residual tumor. In Denmark, postchemotherapy retroperitoneal lumpectomy (RPLP) is performed in patients with residual tumors >1 cm. There is a need to clarify whether this surgical method provides acceptable treatment results. Our objective was to describe morbidity and oncological outcomes of postchemotherapy RPLP. Methods: This was a retrospective population-based multicenter study including patients with nonseminoma testicular cancer and postchemotherapy RPLP performed in Denmark between 1990 and 2015. A total of 219 patients were eligible, with median follow-up of 19 yr. Postoperative complications were evaluated according to the Clavien-Dindo classification. The cumulative incidence of recurrence inside or outside the borders of a bilateral surgical template, progression-free survival (PFS), and overall survival estimates were calculated using the Kaplan-Meier method. Key findings and limitations: After median follow-up of 19 yr, 31/219 patients (14%) experienced a surgical complication, of which 5% were Clavien-Dindo grade ≥III. In total, 37 patients experienced a recurrence. The 5-yr, 10-yr, and 20-yr cumulative risk of recurrence inside a bilateral template was 4.3%, 5.9%, and 5.9%, respectively. The 10-yr PFS rate was 83% and the 10-yr overall survival rate was 96%. The main limitation of the study is the retrospective design. Conclusions and clinical implications: With few patients experiencing a major postoperative complication and a 10-yr cumulative rate of 5.9% for recurrence inside a bilateral surgical template, postchemotherapy RPLP appears to be a safe alternative to template surgery for disseminated nonseminoma. Patient summary: We looked at minimal surgery to remove tumor tissue remaining after chemotherapy in patients with testicular cancer. We found a low frequency of complications, tumor recurrence, and death.
Language	English
Publishing date	2024-01-09
Publishing country	Netherlands
Document type	Journal Article
ISSN	2588-9311
ISSN (online)	2588-9311
DOI	10.1016/j.euo.2023.12.004
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Article ; Online: Factors associated with healing failure after early repair of acute, trauma-related rotator cuff tears.

Aagaard, Knut E / Lunsjö, Karl / Adolfsson, Lars / Frobell, Richard / Björnsson Hallgren, Hanna

Journal of shoulder and elbow surgery

2023 Volume 32, Issue 10, Page(s) 2074–2081

Abstract: Background: Healing failure after rotator cuff repair is a challenging problem. Acute, trauma-related tears are considered a separate entity and are often treated surgically. The aim of this study was to identify factors associated with healing failure ... ...

Abstract	Background: Healing failure after rotator cuff repair is a challenging problem. Acute, trauma-related tears are considered a separate entity and are often treated surgically. The aim of this study was to identify factors associated with healing failure in previously asymptomatic patients with trauma-related rotator cuff tears treated with early arthroscopic repair. Methods: This study included 62 consecutively recruited patients (23% women; median age, 61 years; age range, 42-75 years) with acute symptoms in a previously asymptomatic shoulder and a magnetic resonance imaging-verified full-thickness rotator cuff tear after shoulder trauma. All patients were offered, and underwent, early arthroscopic repair, during which a biopsy specimen was harvested from the supraspinatus tendon and analyzed for signs of degeneration. Of the patients, 57 (92%) completed 1-year follow-up and underwent assessment of repair integrity on magnetic resonance images according to the Sugaya classification. Risk factors for healing failure were investigated using a causal-relation diagram where age, body mass index, tendon degeneration (Bonar score), diabetes mellitus, fatty infiltration (FI), sex, smoking, tear location regarding integrity of the rotator cable, and tear size (number of ruptured tendons and tendon retraction) were included and analyzed. Results: Healing failure at 1 year was identified in 37% of patients (n = 21). A high degree of FI of the supraspinatus muscle (P = .01), a tear location including disruption of rotator cable integrity (P = .01), and old age (P = .03) were associated with healing failure. Tendon degeneration as determined by histopathology was not associated with healing failure at 1-year follow-up (P = .63). Conclusion: Older age, increased FI of the supraspinatus muscle, and a tear including disruption of the rotator cable increased the risk of healing failure after early arthroscopic repair in patients with trauma-related full-thickness rotator cuff tears.
MeSH term(s)	Humans ; Female ; Middle Aged ; Adult ; Aged ; Male ; Rotator Cuff Injuries/diagnostic imaging ; Rotator Cuff Injuries/surgery ; Rotator Cuff Injuries/pathology ; Treatment Outcome ; Rotator Cuff/surgery ; Tendon Injuries/diagnostic imaging ; Tendon Injuries/etiology ; Tendon Injuries/surgery ; Rupture/surgery ; Lacerations ; Magnetic Resonance Imaging ; Arthroscopy/methods
Language	English
Publishing date	2023-05-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	1170782-3
ISSN	1532-6500 ; 1058-2746
ISSN (online)	1532-6500
ISSN	1058-2746
DOI	10.1016/j.jse.2023.03.027
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Zs.A 3973: Show issues

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Article: Subretinal AAV delivery of RNAi-therapeutics targeting

Haldrup, Silja Hansen / Fabian-Jessing, Bjørn K / Jakobsen, Thomas Stax / Lindholm, Anna Bøgh / Adsersen, Rikke L / Aagaard, Lars / Bek, Toke / Askou, Anne Louise / Corydon, Thomas J

Molecular therapy. Methods & clinical development

2024 Volume 32, Issue 2, Page(s) 101242

Abstract: Neovascular age-related macular degeneration (nAMD) is a frequent cause of vision loss among the elderly in the Western world. Current disease management with repeated injections of anti-VEGF agents accumulates the risk for adverse events and constitutes ...

Abstract	Neovascular age-related macular degeneration (nAMD) is a frequent cause of vision loss among the elderly in the Western world. Current disease management with repeated injections of anti-VEGF agents accumulates the risk for adverse events and constitutes a burden for society and the individual patient. Sustained suppression of VEGF using gene therapy is an attractive alternative, which we explored using adeno-associated virus (AAV)-based delivery of novel RNA interference (RNAi) effectors in a porcine model of choroidal neovascularization (CNV). The potency of
Language	English
Publishing date	2024-03-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2872938-9
ISSN	2329-0501 ; 2329-0501
ISSN (online)	2329-0501
ISSN	2329-0501
DOI	10.1016/j.omtm.2024.101242
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Zs.A 7107: Show issues

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Article ; Online: Sex-and age-related variations in myocardial tissue composition of the healthy heart: A native T1 mapping cohort study.

Myhr, Katrine Aagaard / Andrés-Jensen, Liv / Strøier Larsen, Bjørn / Kunkel, Joakim Bo / Kristensen, Charlotte Burup / Vejlstrup, Niels / Køber, Lars / Pecini, Redi

European heart journal. Cardiovascular Imaging

2024

Abstract: Aims: Cardiovascular diseases manifest differently in males and females, potentially influenced by inherent sex- and age-related differences in myocardial tissue composition. Such inherent differences are not well-established in the literature. With ... ...

Abstract	Aims: Cardiovascular diseases manifest differently in males and females, potentially influenced by inherent sex- and age-related differences in myocardial tissue composition. Such inherent differences are not well-established in the literature. With this study using cardiac magnetic resonance (CMR) native T1 mapping, we aim to determine the effect of sex and age on myocardial tissue composition in healthy individuals. Methods and results: CMR native T1 mapping was performed in 276 healthy individuals (55% male, age 8---84 years) on a 1.5 Tesla scanner using a MOLLI 5(3)3 acquisition scheme. Additionally, 30 healthy participants (47% male, age 24-68 years) underwent a 1-year follow-up CMR to assess the longitudinal changes of native T1. Mean native T1 values were 1000±22 ms in males and 1022±23 ms in females (mean difference [MD]=22 ms, 95% CI [17, 27]). Female sex was associated with higher native T1 in multivariable linear regression adjusting for age, heart rate, left ventricular mass index, and blood T1 (β=10 ms, 95% CI [3.4, 15.8]). There was no significant interaction between sex and age (p=0.27). Further, age was not associated with native T1 (β=0.1 ms, 95% CI [-0.02, 0.2]), and native T1 did not change during a 1-year period (MD -4 ms, 95% CI [-11, 3]). Conclusion: Female sex was associated with higher native T1; however, there was no association between age and native T1. Additionally, there was no evidence of an interaction between sex and age. Our findings indicate intrinsic sex-based disparities in myocardial tissue composition.
Language	English
Publishing date	2024-03-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2638345-7
ISSN	2047-2412 ; 2047-2404
ISSN (online)	2047-2412
ISSN	2047-2404
DOI	10.1093/ehjci/jeae070
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Article ; Online: Utility of the Inferior Axis and Left Bundle Branch Block Pattern for Categorization of Patients With Premature Ventricular Complexes Before Catheter Ablation.

Aagaard-Nilsen, Stine / Dejgaard, Lars Andreas / Anfinsen, Ole-Gunnar / Lyseggen, Erik / Holm, Torbjørn / Fink, Trine Synnøve / Odland, Hans Henrik / Sevre, Knut / Kongsgård, Erik / Hegbom, Finn / Stokke, Mathis Korseberg

The American journal of cardiology

2023 Volume 201, Page(s) 148–149

MeSH term(s)	Humans ; Bundle-Branch Block/complications ; Ventricular Premature Complexes/surgery ; Catheter Ablation ; Electrocardiography ; Tachycardia, Ventricular ; Bundle of His
Language	English
Publishing date	2023-06-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	80014-4
ISSN	1879-1913 ; 0002-9149
ISSN (online)	1879-1913
ISSN	0002-9149
DOI	10.1016/j.amjcard.2023.06.013
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Article ; Online: Use and quality of point-of-care microscopy, urine culture and susceptibility testing for urinalysis in general practice.

Kollerup, Ida / Aagaard Thomsen, Anne Kathrine / Kornum, Jette Brommann / Paulsen, Kirsten Inger / Bjerrum, Lars / Hansen, Malene Plejdrup

Scandinavian journal of primary health care

2022 Volume 40, Issue 1, Page(s) 3–10

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Agar ; Anti-Bacterial Agents/therapeutic use ; Escherichia coli ; Female ; General Practice ; Humans ; Male ; Microscopy ; Point-of-Care Systems ; Urinalysis/methods ; Urinary Tract Infections/drug therapy
Chemical Substances	Anti-Bacterial Agents ; Agar (9002-18-0)
Language	English
Publishing date	2022-01-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	605763-9
ISSN	1502-7724 ; 0281-3432 ; 0284-6020
ISSN (online)	1502-7724
ISSN	0281-3432 ; 0284-6020
DOI	10.1080/02813432.2021.2022349
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Article ; Online: Physical and psychosocial work environmental risk factors of low-back pain: protocol for a 1 year prospective cohort study.

Bláfoss, Rúni / Aagaard, Per / Andersen, Lars Louis

BMC musculoskeletal disorders

2019 Volume 20, Issue 1, Page(s) 626

Abstract: Background: Musculoskeletal disorders, and in particular low-back pain (LBP), are common among blue collar workers. In the work environment, both physical- and psychosocial risk factors exist. Working in warehouses in Denmark involve large quantities of ...

Abstract	Background: Musculoskeletal disorders, and in particular low-back pain (LBP), are common among blue collar workers. In the work environment, both physical- and psychosocial risk factors exist. Working in warehouses in Denmark involve large quantities of occupational lifting, high work pace and a low degree of influence at work. This study investigates both acute and long-term associations between physical- and psychosocial work environmental factors and risk of LBP in warehouse workers. The specific study aims are to investigate 1) exposure-response associations between quantity of occupational lifting and short-term (day-to-day) changes in LBP, 2) the influence of accumulated workdays and rest days during a working week on LBP, 3) long-term association between occupational lifting exposure and LBP when assessed over 1 year, and 4) the role of psychological and social factors on the above associations. Methods: The present study is designed as a 1-year prospective cohort study that will examine full-time warehouse workers from up to five retail chains in Denmark. Study aims 1 and 2 will be addressed using objective data based on company records with information on weight of all the goods handled by each warehouse worker during every single workday for 3 weeks. During this period, each worker will reply to text messages received before and after every workday (also on days off work) in which study participants will score their pain in the low back, bodily fatigue and perceived mental stress (scale 0-10). Long-term pain development is assessed using questionnaire surveys before and after 1 year. Further, pressure pain threshold (PPT) will be measured for selected trunk extensor muscles in approximately 50 workers using algometry along with measurements of maximal trunk extensor strength. Associations are modelled using linear mixed models with repeated measures between variables and LBP controlled for relevant confounders. Discussion: This study provides knowledge about the acute and long-term associations between physical- and psychosocial work environmental factors and LBP. The obtained data will have the potential to provide recommendations on improved design of the working week to minimize the risk of LBP among warehouse workers, and may potentially enable to identify a reasonable maximum lifting threshold per day (ton lifted/day).
MeSH term(s)	Adult ; Denmark/epidemiology ; Female ; Humans ; Lifting/adverse effects ; Low Back Pain/diagnosis ; Low Back Pain/epidemiology ; Low Back Pain/etiology ; Low Back Pain/psychology ; Male ; Occupational Exposure/adverse effects ; Pain Measurement ; Prospective Studies ; Research Design ; Risk Factors ; Surveys and Questionnaires ; Workplace/psychology
Language	English
Publishing date	2019-12-27
Publishing country	England
Document type	Journal Article
ISSN	1471-2474
ISSN (online)	1471-2474
DOI	10.1186/s12891-019-2996-z
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Article: Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation.

Chen, Li / Shi, Kaikai / Qiu, Weimin / Aagaard, Lars / Kassem, Moustapha

Stem cells international

2020 Volume 2020, Page(s) 8857344

Abstract: Background: Human bone marrow stromal/stem cells (hMSCs, also known as the skeletal stem cells or mesenchymal stem cells) are being employed to study lineage fate determination to osteoblasts, adipocytes, and chondrocytes. However, mechanistic studies ... ...

Abstract	Background: Human bone marrow stromal/stem cells (hMSCs, also known as the skeletal stem cells or mesenchymal stem cells) are being employed to study lineage fate determination to osteoblasts, adipocytes, and chondrocytes. However, mechanistic studies employing hMSC have been hampered by the difficulty of deriving genetically modified cell lines due to the low and unstable transfection efficiency. Methods: We infected hMSC with a CRISPR/Cas9 lentivirus system, with specific inducible dCas9-coupled transcription activator or repressor: dCas9-KRAB or dCas9-VP64, respectively, and established two hMSC lines (hMSC-CRISPRi and hMSC-CRISPRa) that can inhibit or activate gene expression, respectively. The two cell lines showed similar cell morphology, cell growth kinetics, and similar lineage differentiation potentials as the parental hMSC line. The expression of KRAB-dCas9 or VP64-dCas9 was controlled by the presence or absence of doxycycline (Dox) in the cell culturing medium. To demonstrate the functionality of the dCas9-effector hMSC system, we tested controlled expression of alkaline phosphatase (ALP) gene through transfection with the same single ALP sgRNA. Results: In the presence of Dox, the expression of ALP showed 60-90% inhibition in hMSC-CRISPRi while ALP showed more than 20-fold increased expression in hMSC-CRISPRa. As expected, the ALP was functionally active and the cells showed evidence for inhibition or enhancement of in vitro osteoblast differentiation, respectively. Conclusion: hMSC-CRISPRi and hMSC-CRISPRa are useful resources to study genes and genetic pathways regulating lineage-specific differentiation of hMSC.
Language	English
Publishing date	2020-08-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2573856-2
ISSN	1687-9678 ; 1687-966X
ISSN (online)	1687-9678
ISSN	1687-966X
DOI	10.1155/2020/8857344
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