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  1. Article: Perioperative management of metformin: is there something new?

    Linhartová, Alena / Murínová, Irena / Kovačič, Michaela / Gregorová, Jana

    Casopis lekaru ceskych

    2020  Volume 159, Issue 6, Page(s) 210–212

    Abstract: Metformin is the drug of choice in the treatment of type 2 diabetes mellitus. Diabetic patients treated with metformin, who are scheduled for surgery, have always been subjects to discussion regarding the perioperative management of metformin because of ... ...

    Title translation Perioperační podávání metforminu: co je nového?
    Abstract Metformin is the drug of choice in the treatment of type 2 diabetes mellitus. Diabetic patients treated with metformin, who are scheduled for surgery, have always been subjects to discussion regarding the perioperative management of metformin because of the risk of developing lactic acidosis. The article presents the basic informations about this adverse event, describes new approach to the metformin treatment in the perioperative period, and summarizes the recommendations of professional societies and expert groups.
    MeSH term(s) Acidosis, Lactic ; Diabetes Mellitus, Type 2/drug therapy ; Humans ; Hypoglycemic Agents/adverse effects ; Metformin/adverse effects ; Perioperative Period
    Chemical Substances Hypoglycemic Agents ; Metformin (9100L32L2N)
    Language English
    Publishing date 2020-12-07
    Publishing country Czech Republic
    Document type Journal Article
    ZDB-ID 413441-2
    ISSN 1805-4420 ; 0008-7335
    ISSN (online) 1805-4420
    ISSN 0008-7335
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Delivery of

    Badami, Giusto D / La Manna, Marco P / Di Carlo, Paola / Stanek, Ondrej / Linhartova, Irena / Caccamo, Nadia / Sebo, Peter / Dieli, Francesco

    Frontiers in immunology

    2023  Volume 14, Page(s) 1289212

    Abstract: Introduction: Tuberculosis (TB) remains the first cause of death from infection caused by a bacterial pathogen. Chemotherapy does not eradicate : Methods: The enzymatically inactive Bordetella pertussis adenylate cyclase (CyaA) toxoid is an effective ...

    Abstract Introduction: Tuberculosis (TB) remains the first cause of death from infection caused by a bacterial pathogen. Chemotherapy does not eradicate
    Methods: The enzymatically inactive Bordetella pertussis adenylate cyclase (CyaA) toxoid is an effective tool for delivering peptide epitopes into the cytosol of antigen-presenting cells (APC) for presentation and stimulation of specific CD8+ T-cell responses. In this study, we have investigated the capacity of the CyaA toxoid to deliver Mtb epitopes known to bind HLA-E for the expansion of human CD8+ T cells
    Results: Our results show that the CyaA-toxoid containing five HLA-E-restricted Mtb epitopes causes significant expansion of HLA-E-restricted antigen-specific CD8+ T cells, which produce IFN-γ and exert significant cytotoxic activity towards peptide-pulsed macrophages.
    Discussion: HLA-E represents a promising platform for the development of new vaccines; our study indicates that the CyaA construct represents a suitable delivery system of the HLA-E-binding Mtb epitopes for
    MeSH term(s) Humans ; Adenylyl Cyclases ; Bordetella pertussis ; CD8-Positive T-Lymphocytes ; Epitopes ; Histocompatibility Antigens Class I ; HLA-E Antigens ; Mycobacterium tuberculosis ; Peptides ; Toxoids ; Tuberculosis/prevention & control
    Chemical Substances Adenylyl Cyclases (EC 4.6.1.1) ; Epitopes ; Histocompatibility Antigens Class I ; HLA-E Antigens ; Peptides ; Toxoids
    Language English
    Publishing date 2023-12-01
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1289212
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: A Mutation Upstream of the

    Novák, Jakub / Jurnečka, David / Linhartová, Irena / Holubová, Jana / Staněk, Ondřej / Štipl, Daniel / Dienstbier, Ana / Večerek, Branislav / Azevedo, Nayara / Provazník, Jan / Beneš, Vladimír / Šebo, Peter

    mSystems

    2020  Volume 5, Issue 6

    Abstract: The BvgS/BvgA two-component system controls expression of ∼550 genes ... ...

    Abstract The BvgS/BvgA two-component system controls expression of ∼550 genes of
    Language English
    Publishing date 2020-12-08
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5077
    ISSN 2379-5077
    DOI 10.1128/mSystems.00612-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Phosphoproteomics of cAMP signaling of Bordetella adenylate cyclase toxin in mouse dendritic cells.

    Novák, Jakub / Fabrik, Ivo / Linhartová, Irena / Link, Marek / Černý, Ondřej / Stulík, Jiří / Šebo, Peter

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 16298

    Abstract: The adenylate cyclase toxin (CyaA) of the whooping cough agent Bordetella pertussis subverts immune functions of host myeloid cells expressing the ... ...

    Abstract The adenylate cyclase toxin (CyaA) of the whooping cough agent Bordetella pertussis subverts immune functions of host myeloid cells expressing the α
    MeSH term(s) Animals ; Bordetella pertussis/metabolism ; Cell Adhesion Molecules/metabolism ; Cyclic AMP/metabolism ; Cytoskeletal Proteins/metabolism ; Dendritic Cells/metabolism ; Female ; Histone Deacetylases/metabolism ; Mice ; Mice, Inbred C57BL ; Microfilament Proteins/metabolism ; Phosphoproteins/metabolism ; Signal Transduction/physiology ; Talin/metabolism ; Transcription Factors/metabolism ; Whooping Cough/microbiology
    Chemical Substances CRTC3 protein, mouse ; Cell Adhesion Molecules ; Cytoskeletal Proteins ; Enah protein, mouse ; Microfilament Proteins ; Phosphoproteins ; Talin ; Transcription Factors ; Tln1 protein, mouse ; vasodilator-stimulated phosphoprotein ; Cyclic AMP (E0399OZS9N) ; Hdac5 protein, mouse (EC 3.5.1.98) ; Histone Deacetylases (EC 3.5.1.98)
    Language English
    Publishing date 2017-11-24
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-14501-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Transcriptional profiling of human macrophages during infection with

    Petráčková, Denisa / Farman, Mariam R / Amman, Fabian / Linhartová, Irena / Dienstbier, Ana / Kumar, Dilip / Držmíšek, Jakub / Hofacker, Ivo / Rodriguez, Maria Eugenia / Večerek, Branislav

    RNA biology

    2020  Volume 17, Issue 5, Page(s) 731–742

    Abstract: Bordetella ... ...

    Abstract Bordetella pertussis
    MeSH term(s) Bordetella pertussis/physiology ; Cell Line ; Cells, Cultured ; Computational Biology/methods ; Gene Expression Profiling/methods ; Gene Expression Regulation ; Gene Ontology ; Gene Regulatory Networks ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/immunology ; Humans ; Macrophages/immunology ; Macrophages/metabolism ; Macrophages/microbiology ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Transcriptome ; Whooping Cough/genetics ; Whooping Cough/immunology ; Whooping Cough/virology
    Language English
    Publishing date 2020-02-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2159587-2
    ISSN 1555-8584 ; 1555-8584
    ISSN (online) 1555-8584
    ISSN 1555-8584
    DOI 10.1080/15476286.2020.1727694
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Production of Highly Active Recombinant Dermonecrotic Toxin of

    Stanek, Ondrej / Linhartova, Irena / Holubova, Jana / Bumba, Ladislav / Gardian, Zdenko / Malandra, Anna / Bockova, Barbora / Teruya, Shihono / Horiguchi, Yasuhiko / Osicka, Radim / Sebo, Peter

    Toxins

    2020  Volume 12, Issue 9

    Abstract: ... ...

    Abstract Pathogenic
    MeSH term(s) 3T3 Cells ; A549 Cells ; Animals ; Animals, Newborn ; Bordetella pertussis/enzymology ; Bordetella pertussis/genetics ; Bordetella pertussis/pathogenicity ; Calcium Channels, T-Type/genetics ; Calcium Channels, T-Type/metabolism ; Epithelial Cells/metabolism ; Epithelial Cells/ultrastructure ; Humans ; Mice ; Mice, Inbred BALB C ; Necrosis ; Protein Binding ; Protein Domains ; Recombinant Proteins/metabolism ; Skin/drug effects ; Skin/pathology ; Structure-Activity Relationship ; Transglutaminases/genetics ; Transglutaminases/metabolism ; Transglutaminases/toxicity ; Transglutaminases/ultrastructure ; Virulence Factors, Bordetella/genetics ; Virulence Factors, Bordetella/metabolism ; Virulence Factors, Bordetella/toxicity
    Chemical Substances CACNA1G protein, human ; CACNA1H protein, human ; Calcium Channels, T-Type ; Recombinant Proteins ; Virulence Factors, Bordetella ; dermonecrotic toxin, Bordetella ; Transglutaminases (EC 2.3.2.13)
    Language English
    Publishing date 2020-09-15
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins12090596
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Bordetella pertussis Adenylate Cyclase Toxin Disrupts Functional Integrity of Bronchial Epithelial Layers.

    Hasan, Shakir / Kulkarni, Nikhil Nitin / Asbjarnarson, Arni / Linhartova, Irena / Osicka, Radim / Sebo, Peter / Gudmundsson, Gudmundur H

    Infection and immunity

    2018  Volume 86, Issue 3

    Abstract: The airway epithelium restricts the penetration of inhaled pathogens into the underlying tissue and plays a crucial role in the innate immune defense against respiratory infections. The whooping cough agent, ...

    Abstract The airway epithelium restricts the penetration of inhaled pathogens into the underlying tissue and plays a crucial role in the innate immune defense against respiratory infections. The whooping cough agent,
    MeSH term(s) Adenylate Cyclase Toxin/genetics ; Adenylate Cyclase Toxin/metabolism ; Adenylate Cyclase Toxin/toxicity ; Bordetella pertussis/genetics ; Bordetella pertussis/metabolism ; Bronchi/cytology ; Bronchi/metabolism ; Bronchi/microbiology ; Cyclic AMP/metabolism ; Cytoskeleton/metabolism ; Epithelial Cells/metabolism ; Epithelial Cells/microbiology ; Humans ; Interleukin-6/metabolism ; Mucin 5AC/metabolism ; Signal Transduction/drug effects ; Whooping Cough/genetics ; Whooping Cough/metabolism ; Whooping Cough/microbiology
    Chemical Substances Adenylate Cyclase Toxin ; Interleukin-6 ; Mucin 5AC ; Cyclic AMP (E0399OZS9N)
    Language English
    Publishing date 2018-02-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/IAI.00445-17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: cAMP signalling of Bordetella adenylate cyclase toxin through the SHP-1 phosphatase activates the BimEL-Bax pro-apoptotic cascade in phagocytes.

    Ahmad, Jawid Nazir / Cerny, Ondrej / Linhartova, Irena / Masin, Jiri / Osicka, Radim / Sebo, Peter

    Cellular microbiology

    2016  Volume 18, Issue 3, Page(s) 384–398

    Abstract: The adenylate cyclase toxin-hemolysin (CyaA, ACT or AC-Hly) plays a key role in virulence of Bordetella pertussis. CyaA penetrates myeloid cells expressing the complement receptor 3 (αM β2 integrin CD11b/CD18) and subverts bactericidal capacities of ... ...

    Abstract The adenylate cyclase toxin-hemolysin (CyaA, ACT or AC-Hly) plays a key role in virulence of Bordetella pertussis. CyaA penetrates myeloid cells expressing the complement receptor 3 (αM β2 integrin CD11b/CD18) and subverts bactericidal capacities of neutrophils and macrophages by catalysing unregulated conversion of cytosolic ATP to the key signalling molecule adenosine 3',5'-cyclic monophosphate (cAMP). We show that the signalling of CyaA-produced cAMP hijacks, by an as yet unknown mechanism, the activity of the tyrosine phosphatase SHP-1 and activates the pro-apoptotic BimEL-Bax cascade. Mitochondrial hyperpolarization occurred in human THP-1 macrophages within 10 min of exposure to low CyaA concentrations (e.g. 20 ng ml(-1) ) and was accompanied by accumulation of BimEL and association of the pro-apoptotic factor Bax with mitochondria. BimEL accumulation required cAMP/protein kinase A signalling, depended on SHP-1 activity and was selectively inhibited upon small interfering RNA knockdown of SHP-1 but not of the SHP-2 phosphatase. Moreover, signalling of CyaA-produced cAMP inhibited the AKT/protein kinase B pro-survival cascade, enhancing activity of the FoxO3a transcription factor and inducing Bim transcription. Synergy of FoxO3a activation with SHP-1 hijacking thus enables the toxin to rapidly trigger a persistent accumulation of BimEL, thereby activating the pro-apoptotic programme of macrophages and subverting the innate immunity of the host.
    MeSH term(s) Adenylate Cyclase Toxin/metabolism ; Apoptosis/physiology ; Apoptosis Regulatory Proteins/metabolism ; Bcl-2-Like Protein 11 ; Bordetella pertussis/metabolism ; Bordetella pertussis/pathogenicity ; Cyclic AMP/metabolism ; Forkhead Box Protein O3 ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/metabolism ; Host-Pathogen Interactions/physiology ; Humans ; Macrophages/metabolism ; Membrane Proteins/metabolism ; Mitochondria/metabolism ; Phagocytes/metabolism ; Phagocytes/microbiology ; Protein Tyrosine Phosphatase, Non-Receptor Type 6/metabolism ; Proto-Oncogene Proteins/metabolism ; Signal Transduction ; bcl-2-Associated X Protein/metabolism
    Chemical Substances Adenylate Cyclase Toxin ; Apoptosis Regulatory Proteins ; BAX protein, human ; Bcl-2-Like Protein 11 ; FOXO3 protein, human ; Forkhead Box Protein O3 ; Forkhead Transcription Factors ; Membrane Proteins ; Proto-Oncogene Proteins ; bcl-2-Associated X Protein ; Cyclic AMP (E0399OZS9N) ; PTPN6 protein, human (EC 3.1.3.48) ; Protein Tyrosine Phosphatase, Non-Receptor Type 6 (EC 3.1.3.48)
    Language English
    Publishing date 2016-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1468320-9
    ISSN 1462-5822 ; 1462-5814
    ISSN (online) 1462-5822
    ISSN 1462-5814
    DOI 10.1111/cmi.12519
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Structure-Function Relationships Underlying the Capacity of Bordetella Adenylate Cyclase Toxin to Disarm Host Phagocytes.

    Novak, Jakub / Cerny, Ondrej / Osickova, Adriana / Linhartova, Irena / Masin, Jiri / Bumba, Ladislav / Sebo, Peter / Osicka, Radim

    Toxins

    2017  Volume 9, Issue 10

    Abstract: ... ...

    Abstract Bordetellae
    MeSH term(s) Adenylate Cyclase Toxin/chemistry ; Animals ; Bordetella pertussis ; Cyclic AMP/chemistry ; Dendritic Cells/cytology ; Humans ; Macrophage-1 Antigen ; Macrophages, Alveolar/cytology ; Neutrophils/cytology ; Phagocytes/chemistry ; Protein Domains ; Protein Structure, Tertiary ; Signal Transduction ; Structure-Activity Relationship ; Syk Kinase
    Chemical Substances Adenylate Cyclase Toxin ; Macrophage-1 Antigen ; Cyclic AMP (E0399OZS9N) ; SYK protein, human (EC 2.7.10.2) ; Syk Kinase (EC 2.7.10.2)
    Language English
    Publishing date 2017-09-24
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins9100300
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Production of Highly Active Recombinant Dermonecrotic Toxin of Bordetella Pertussis

    Ondrej Stanek / Irena Linhartova / Jana Holubova / Ladislav Bumba / Zdenko Gardian / Anna Malandra / Barbora Bockova / Shihono Teruya / Yasuhiko Horiguchi / Radim Osicka / Peter Sebo

    Toxins, Vol 12, Iss 596, p

    2020  Volume 596

    Abstract: Pathogenic Bordetella bacteria release a neurotropic dermonecrotic toxin (DNT) that is endocytosed into animal cells and permanently activates the Rho family GTPases by polyamination or deamidation of the glutamine residues in their switch II regions (e ... ...

    Abstract Pathogenic Bordetella bacteria release a neurotropic dermonecrotic toxin (DNT) that is endocytosed into animal cells and permanently activates the Rho family GTPases by polyamination or deamidation of the glutamine residues in their switch II regions (e.g., Gln63 of RhoA). DNT was found to enable high level colonization of the nasal cavity of pigs by B. bronchiseptica and the capacity of DNT to inhibit differentiation of nasal turbinate bone osteoblasts causes atrophic rhinitis in infected pigs. However, it remains unknown whether DNT plays any role also in virulence of the human pathogen B. pertussis and in pathogenesis of the whooping cough disease. We report a procedure for purification of large amounts of LPS-free recombinant DNT that exhibits a high biological activity on cells expressing the DNT receptors Cav3.1 and Cav3.2Electron microscopy and single particle image analysis of negatively stained preparations revealed that the DNT molecule adopts a V-shaped structure with well-resolved protein domains. These results open the way to structure–function studies on DNT and its interactions with airway epithelial layers.
    Keywords Bordetella ; GTPase ; deamidation ; dermonecrotic toxin ; recombinant ; electron microscopy ; negative staining ; image analysis ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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