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Article ; Online: Feed withdrawal influences egg production, egg quality and antioxidant variables in White Leghorn layers reared during the tropical summer season.

Rama Rao, S V / Raju, M V L N / Rajkumar, U / Prakash, B / Kumar, P S P

2023 Volume 64, Issue 4, Page(s) 497–503

Abstract: 1. An experiment was conducted with White Leghorn layers (Babcock BV 300) to optimise the feed withdrawal schedule for egg-laying hens (67 to 80 weeks) reared in open-sided poultry houses during the tropical summer season.2. Four feeding schedules (1. ...

Abstract	1. An experiment was conducted with White Leghorn layers (Babcock BV 300) to optimise the feed withdrawal schedule for egg-laying hens (67 to 80 weeks) reared in open-sided poultry houses during the tropical summer season.2. Four feeding schedules (1.
MeSH term(s)	Animals ; Female ; Antioxidants ; Chickens/physiology ; Diet/veterinary ; Seasons ; Poultry ; Animal Feed/analysis ; Oviposition ; Eggs
Chemical Substances	Antioxidants
Language	English
Publishing date	2023-05-31
Publishing country	England
Document type	Journal Article
ZDB-ID	956653-3
ISSN	1466-1799 ; 0007-1668
ISSN (online)	1466-1799
ISSN	0007-1668
DOI	10.1080/00071668.2023.2208266
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Article ; Online: Attenuation of obesity related inflammation in RAW 264.7 macrophages and 3T3-L1 adipocytes by varanadi kashayam and identification of potential bioactive molecules by UHPLC-Q-Orbitrap HRMS.

Chinchu, J U / Mohan, Mohind C / Prakash Kumar, B

Archives of physiology and biochemistry

2021 Volume 129, Issue 4, Page(s) 879–892

Abstract: Obesity is associated with chronic low-grade inflammation, characterised by the up-regulation of pro-inflammatory cytokines in obese adipose tissue. In this study, 3T3-L1 CM enhanced TNF-α and IL-1β in RAW 264.7 cells compared with LPS treated cells. ... ...

Abstract	Obesity is associated with chronic low-grade inflammation, characterised by the up-regulation of pro-inflammatory cytokines in obese adipose tissue. In this study, 3T3-L1 CM enhanced TNF-α and IL-1β in RAW 264.7 cells compared with LPS treated cells. However, treatment with Varanadi kashayam suppressed the inflammatory changes associated with RAW 264.7 cells. Subsequently, RAW CM used to stimulate adipocytes, resulting in decreased intracellular lipid content and reduced adipogenic markers after Varanadi kashayam treatment. The chemical profiling of Varanadi kashayam using UHPLC-Q-Orbitrap-HRMS identified 194 compounds by comparing their retention time, the experimentally measured exact mass of precursor, and fragmented ions, and fragmentation pattern with spectral library and reported literature. Collectively, Varanadi kashayam act as a potent anti-inflammatory and anti-adipogenic agent that could disrupt the crosstalk between adipocytes and macrophages. Hence it could be a better candidate for reducing inflammation associated with obese adipose tissue.
MeSH term(s)	Animals ; Mice ; 3T3-L1 Cells ; Chromatography, High Pressure Liquid ; Inflammation ; Macrophages ; Obesity ; Adipocytes
Language	English
Publishing date	2021-02-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1238320-x
ISSN	1744-4160 ; 1381-3455
ISSN (online)	1744-4160
ISSN	1381-3455
DOI	10.1080/13813455.2021.1877309
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Article: Automated semantic lung segmentation in chest CT images using deep neural network.

Murugappan, M / Bourisly, Ali K / Prakash, N B / Sumithra, M G / Acharya, U Rajendra

Neural computing & applications

2023 Volume 35, Issue 21, Page(s) 15343–15364

Abstract: Lung segmentation algorithms play a significant role in segmenting theinfected regions in the lungs. This work aims to develop a computationally efficient and robust deep learning model for lung segmentation using chest computed tomography (CT) images ... ...

Abstract	Lung segmentation algorithms play a significant role in segmenting theinfected regions in the lungs. This work aims to develop a computationally efficient and robust deep learning model for lung segmentation using chest computed tomography (CT) images with DeepLabV3 + networks for two-class (background and lung field) and four-class (ground-glass opacities, background, consolidation, and lung field). In this work, we investigate the performance of the DeepLabV3 + network with five pretrained networks: Xception, ResNet-18, Inception-ResNet-v2, MobileNet-v2 and ResNet-50. A publicly available database for COVID-19 that contains 750 chest CT images and corresponding pixel-labeled images are used to develop the deep learning model. The segmentation performance has been assessed using five performance measures: Intersection of Union (IoU), Weighted IoU, Balance F1 score, pixel accu-racy, and global accuracy. The experimental results of this work confirm that the DeepLabV3 + network with ResNet-18 and a batch size of 8 have a higher performance for two-class segmentation. DeepLabV3 + network coupled with ResNet-50 and a batch size of 16 yielded better results for four-class segmentation compared to other pretrained networks. Besides, the ResNet with a fewer number of layers is highly adequate for developing a more robust lung segmentation network with lesser computational complexity compared to the conventional DeepLabV3 + network with Xception. This present work proposes a unified DeepLabV3 + network to delineate the two and four different regions automatically using CT images for CoVID-19 patients. Our developed automated segmented model can be further developed to be used as a clinical diagnosis system for CoVID-19 as well as assist clinicians in providing an accurate second opinion CoVID-19 diagnosis.
Language	English
Publishing date	2023-04-10
Publishing country	England
Document type	Journal Article
ZDB-ID	1480526-1
ISSN	1433-3058 ; 0941-0643
ISSN (online)	1433-3058
ISSN	0941-0643
DOI	10.1007/s00521-023-08407-1
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Article ; Online: Effortless residual adhesive removal: A simple method.

Saha, Bipasa / Ansari, Reyaz / Prakash, Krishna / De, Utpal

Tropical doctor

2023 Volume 53, Issue 4, Page(s) 492–493

Abstract: The cheaper surgical dressings often leave residual adhesive, which can be unsightly and uncomfortable. We describe a simple and cost-effective procedure for removing adhesive residue using micropore tape. This technique can be easily performed by anyone, ...

Abstract	The cheaper surgical dressings often leave residual adhesive, which can be unsightly and uncomfortable. We describe a simple and cost-effective procedure for removing adhesive residue using micropore tape. This technique can be easily performed by anyone, including the patients themselves.
MeSH term(s)	Humans ; Adhesives ; Bandages
Chemical Substances	Adhesives
Language	English
Publishing date	2023-05-19
Publishing country	England
Document type	Journal Article
ZDB-ID	193169-6
ISSN	1758-1133 ; 0049-4755
ISSN (online)	1758-1133
ISSN	0049-4755
DOI	10.1177/00494755231177093
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Article: Staphylococcous epidermidis

Abraham, Betsy / Gokhale, Antara U / Mohsin, Jalila / Prakash, Sadanandan

Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine

2020 Volume 24, Issue 8, Page(s) 716–718

Abstract: Coagulase-negative : How to cite this article: Abraham B, Gokhale AU, Mohsin J, Prakash S. ...

Abstract	Coagulase-negative How to cite this article: Abraham B, Gokhale AU, Mohsin J, Prakash S.
Language	English
Publishing date	2020-10-06
Publishing country	India
Document type	Case Reports
ZDB-ID	2121263-6
ISSN	1998-359X ; 0972-5229
ISSN (online)	1998-359X
ISSN	0972-5229
DOI	10.5005/jp-journals-10071-23523
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Article ; Online: Transoral retroauricular neck dissection (TREND): A novel combination approach.

Kudpaje, A / BhanuPrakash, B / Thakur, S / Arakeri, G / Rao, V U S

International journal of oral and maxillofacial surgery

2021 Volume 51, Issue 4, Page(s) 459–462

Abstract: Early-stage oral squamous cell carcinoma is treated preferably by wide local tumour excision along with elective neck dissection. The conventional neck dissection leaves an unaesthetic scar, which remains a major challenge that adversely impacts patient ... ...

Abstract	Early-stage oral squamous cell carcinoma is treated preferably by wide local tumour excision along with elective neck dissection. The conventional neck dissection leaves an unaesthetic scar, which remains a major challenge that adversely impacts patient satisfaction, their social interactions, and quality of life (QoL). In recent times, retroauricular assisted endoscopic and robotic neck dissection techniques that avoid unaesthetic neck scars have gained popularity. The pitfalls in attaining universal acceptance of these techniques are the need for specialized instrumentation, training, and increased costs. The need for an endoscope or robotic camera when using the retroauricular approach arises mainly while addressing the level I lymph nodes, due to poor access. A combination of transoral and retroauricular approaches that overcomes these factors is presented here, named the transoral retroauricular neck dissection (TREND). The technique successfully avoids a visible neck scar while providing adequate exposure of level I lymph nodes without the need for specialized instrumentation. This approach has been applied, with adequate lymph node clearance achieved in all patients. This novel combination approach of neck dissection is oncologically safe, easy to replicate, and improves patient aesthetics, functional outcomes, and QoL. We recommend that clinicians practice this simple technique and enhance the practice of remote access neck dissection.
MeSH term(s)	Carcinoma, Squamous Cell/pathology ; Carcinoma, Squamous Cell/surgery ; Esthetics, Dental ; Head and Neck Neoplasms/surgery ; Humans ; Mouth Neoplasms/surgery ; Neck Dissection/methods ; Quality of Life
Language	English
Publishing date	2021-08-08
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	353721-3
ISSN	1399-0020 ; 0901-5027
ISSN (online)	1399-0020
ISSN	0901-5027
DOI	10.1016/j.ijom.2021.07.009
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Article ; Online: Pharmacophore-guided drug design using LdNMT as a model drug target for leishmaniasis.

Sooram, Banesh / Mallikarjunachari, Uppuladinne / Uddavesh, Sonavane / Saudagar, Prakash

Journal of biomolecular structure & dynamics

2023 Volume 42, Issue 2, Page(s) 863–875

Abstract: Leishmaniasis is caused ... ...

Abstract	Leishmaniasis is caused by
MeSH term(s)	Humans ; Leishmania ; Transferases ; Pharmacophore ; Myristates ; Leishmaniasis/drug therapy ; Drug Design ; Alanine ; Amino Acids
Chemical Substances	Transferases (EC 2.-) ; Myristates ; Alanine (OF5P57N2ZX) ; Amino Acids
Language	English
Publishing date	2023-04-25
Publishing country	England
Document type	Journal Article
ZDB-ID	49157-3
ISSN	1538-0254 ; 0739-1102
ISSN (online)	1538-0254
ISSN	0739-1102
DOI	10.1080/07391102.2023.2196695
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Article ; Online: Feed withdrawal influences egg production, egg quality and antioxidant variables in White Leghorn layers reared during the tropical summer season

Rama Rao, S. V. / Raju, M. V. L. N. / Rajkumar, U. / Prakash, B. / Kumar, P. S. P.

British Poultry Science. 2023 July 04, v. 64, no. 4 p.497-503

2023

Abstract	1. An experiment was conducted with White Leghorn layers (Babcock BV 300) to optimise the feed withdrawal schedule for egg-laying hens (67 to 80 weeks) reared in open-sided poultry houses during the tropical summer season. 2. Four feeding schedules (1. ad libitum access to the layer feed, 2. feed withdrawal from 9 AM-5 PM, 3. 11 AM-5 PM, and 4. 1 PM-5 PM followed by free access to the layer feed) were examined. Each feeding schedule was offered to 12 replicates consisting of 12 layers housed in four adjacent cages (45.7 × 35.6 × 43.2 cm). 3. Fasting the layers from 1-5 pm reduced egg production compared to layers fasting from 9 or 11 am. The FI/egg or unit egg mass was highest in layers fasted from 1-5 pm. In the 9 am-5 pm fasted group, the cloacal temperature (CT) increased significantly at 11 am and gradually reduced at 3 pm. In the 1-5 pm fasted group, the CT remained higher at 3 pm and 5 pm. 4. The cell-mediated immune response in layers fasted from 9 am or 11 am was higher than those fed ad libitum or 1-5 pm fasted group. Lipid peroxidation was higher in layers 1-5 pm fasted group, and significantly lower LP was found in groups that fasted from 9 am or 11 am. In general, the activities of serum antioxidant enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase) were higher in layers that fasted from 9 am or 11 am compared to those that had ad libitum feed or fasted from 1-5 pm. 5. It was concluded that the feed withdrawal should be initiated during the forenoon (9 AM/11 am-5 pm) for optimum production and to reduce oxidative stress in egg-laying hens reared during summer in the tropical region.
Keywords	White Leghorn ; ad libitum feeding ; antioxidants ; blood serum ; cell-mediated immunity ; cloaca ; egg masses ; egg production ; egg quality ; eggs ; feed deprivation ; glutathione peroxidase ; glutathione-disulfide reductase ; lipid peroxidation ; oxidative stress ; poultry ; summer ; superoxide dismutase ; temperature ; tropics ; Feed withdrawal ; antioxidant ; layers ; tropical summer
Language	English
Dates of publication	2023-0704
Size	p. 497-503.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	956653-3
ISSN	1466-1799 ; 0007-1668
ISSN (online)	1466-1799
ISSN	0007-1668
DOI	10.1080/00071668.2023.2208266
Database	NAL-Catalogue (AGRICOLA)

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Article: Safety and Efficacy of Genicular Artery Embolization for the Treatment of Knee Pain Secondary to Osteoarthritis-Initial Indian Experience.

Gorsi, Ujjwal / Singh, Damandeep / Kang, Mandeep / Kumar, Vishal / Prakash, Mahesh / B C, Sreedhara

The Indian journal of radiology & imaging

2023 Volume 34, Issue 1, Page(s) 37–43

Abstract: ... ...

Abstract	Background
Language	English
Publishing date	2023-08-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	605869-3
ISSN	0971-3026 ; 0970-2016
ISSN	0971-3026 ; 0970-2016
DOI	10.1055/s-0043-1771159
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Article ; Online: Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle.

Alyethodi, R R / Singh, U / Kumar, S / Alex, R / Sengar, G S / Raja, T V / Deb, R / Prakash, B

BMC biotechnology

2021 Volume 21, Issue 1, Page(s) 36

Abstract: Background: DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific ... ...

Abstract	Background: DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific genotyping protocol is followed. Various researches are focused on overcoming these issues. Microcards offer blood transportation at ambient temperature. Direct PCR methods can save the time of DNA extraction but available only for simplex PCR. Tetra Primer-Amplification Refractory Mutation System based Polymerase Chain Reaction (T-ARMS PCR) can make DNA testing faster in a low-cost setting. The present study was aimed to design, optimize, and validate a T-ARMS PCR for faster DNA testing of SNP responsible for Complex Vertebral Malformation (CVM)-an important genetic disease of the cattle industry. Further, a direct T-ARMS PCR from whole blood was developed to avoid the DNA extraction steps. Lastly, using the optimized protocol, genotyping of blood spotted on Microcard eliminates the need for cold chain maintenance in the transportation of samples. Results: The present study demonstrated a novel T-ARMS PCR-based genotyping of the SNP rs438228855, which is responsible for CVM. Here, wild genotypes were recognized by 389 bp and 199 bp bands in agarose gel, while the carrier genotype showed an additional 241 bp band. The developed protocol was validated using PCR-Primer Introduced Restriction Analysis (PCR-PIRA) and sequencing. The present study further established a direct T-ARMS PCR for this SNP from whole blood. Different conditions such as heparin and EDTA treated blood, the need for pre-treatment, and two different DNA Polymerases for the direct PCR were optimized. Finally, our optimized protocol successfully genotyped the whole blood samples dried on Insta™DNA cards. Conclusions: The present study reported the usefulness of primer modified T-ARMS PCR for detecting CVM for the first time. To the best of our knowledge, direct PCR in T-ARMS PCR has never been reported. Lastly, the use of microcards in the developed protocol can make the assay useful in the DNA testing of field samples.
MeSH term(s)	Animals ; Cattle ; Cattle Diseases/blood ; Cattle Diseases/congenital ; Cattle Diseases/diagnosis ; Cattle Diseases/genetics ; DNA/genetics ; DNA Primers/genetics ; Genotype ; Genotyping Techniques/methods ; Polymerase Chain Reaction/methods ; Polymorphism, Single Nucleotide
Chemical Substances	DNA Primers ; DNA (9007-49-2)
Language	English
Publishing date	2021-05-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
ISSN	1472-6750
ISSN (online)	1472-6750
DOI	10.1186/s12896-021-00696-5
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