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  1. Article ; Online: Correction: Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13Rα2 after radiolabeling with

    Leland, Pamela / Kumar, Dhiraj / Nimmagadda, Sridhar / Bauer, Steven R / Puri, Raj K / Joshi, Bharat H

    Journal of translational medicine

    2023  Volume 21, Issue 1, Page(s) 636

    Language English
    Publishing date 2023-09-19
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2118570-0
    ISSN 1479-5876 ; 1479-5876
    ISSN (online) 1479-5876
    ISSN 1479-5876
    DOI 10.1186/s12967-023-04496-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Characterization of Chimeric Antigen Receptor Modified T Cells Expressing scFv-IL-13Rα2 after Radiolabeling with 89Zirconium Oxine for PET Imaging.

    Leland, Pamela / Kumar, Dhiraj / Nimaggada, Sridhar / Bauer, Steven R / Puri, Raj K / Joshi, Bharat H

    Research square

    2023  

    Abstract: Background Chimeric antigen receptor (CAR) T cell therapy is an exciting cell-based cancer immunotherapy. Unfortunately, CAR-T cell therapy is associated with serious toxicities such as cytokine release syndrome (CRS) and neurotoxicity. The mechanism of ... ...

    Abstract Background Chimeric antigen receptor (CAR) T cell therapy is an exciting cell-based cancer immunotherapy. Unfortunately, CAR-T cell therapy is associated with serious toxicities such as cytokine release syndrome (CRS) and neurotoxicity. The mechanism of these serious adverse events (SAEs) and how homing, distribution and retention of CAR-T cells contribute to toxicities is not fully understood. Methods To determine if radiolabelling of CAR-T cells could support positron emission tomography (PET)-based biodistribution studies, we labeled IL-13Rα2 targeting scFv-IL-13Rα2-CAR-T cells (CAR-T cells) with
    Language English
    Publishing date 2023-01-13
    Publishing country United States
    Document type Preprint
    DOI 10.21203/rs.3.rs-2242559/v1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online: Gradient-based Uncertainty Attribution for Explainable Bayesian Deep Learning

    Wang, Hanjing / Joshi, Dhiraj / Wang, Shiqiang / Ji, Qiang

    2023  

    Abstract: Predictions made by deep learning models are prone to data perturbations, adversarial attacks, and out-of-distribution inputs. To build a trusted AI system, it is therefore critical to accurately quantify the prediction uncertainties. While current ... ...

    Abstract Predictions made by deep learning models are prone to data perturbations, adversarial attacks, and out-of-distribution inputs. To build a trusted AI system, it is therefore critical to accurately quantify the prediction uncertainties. While current efforts focus on improving uncertainty quantification accuracy and efficiency, there is a need to identify uncertainty sources and take actions to mitigate their effects on predictions. Therefore, we propose to develop explainable and actionable Bayesian deep learning methods to not only perform accurate uncertainty quantification but also explain the uncertainties, identify their sources, and propose strategies to mitigate the uncertainty impacts. Specifically, we introduce a gradient-based uncertainty attribution method to identify the most problematic regions of the input that contribute to the prediction uncertainty. Compared to existing methods, the proposed UA-Backprop has competitive accuracy, relaxed assumptions, and high efficiency. Moreover, we propose an uncertainty mitigation strategy that leverages the attribution results as attention to further improve the model performance. Both qualitative and quantitative evaluations are conducted to demonstrate the effectiveness of our proposed methods.

    Comment: Accepted to CVPR 2023
    Keywords Computer Science - Machine Learning ; Computer Science - Computer Vision and Pattern Recognition ; Computer Science - Information Theory ; Statistics - Machine Learning
    Subject code 006
    Publishing date 2023-04-10
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Primary omental ectopic pregnancy: A rare case report.

    Budha, Bishal / Jha, Kritika / Poudel, Dhiraj / Pokharel, Saroj / Aryal, Suraj / Bajracharya, Satish / Joshi, Bishweshwar / Ghimire, Asmita

    Clinical case reports

    2023  Volume 11, Issue 10, Page(s) e8063

    Abstract: Primary abdominal pregnancy is the rarest form of ectopic pregnancy in which the fertilized ovum is directly implanted into peritoneal cavity. This condition poses significantly high risk of maternal morbidity and mortality. Here, we present a case of 5 ... ...

    Abstract Primary abdominal pregnancy is the rarest form of ectopic pregnancy in which the fertilized ovum is directly implanted into peritoneal cavity. This condition poses significantly high risk of maternal morbidity and mortality. Here, we present a case of 5 weeks of primary omental pregnancy managed by laparotomy.
    Language English
    Publishing date 2023-10-17
    Publishing country England
    Document type Case Reports
    ZDB-ID 2740234-4
    ISSN 2050-0904
    ISSN 2050-0904
    DOI 10.1002/ccr3.8063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Correction

    Pamela Leland / Dhiraj Kumar / Sridhar Nimmagadda / Steven R. Bauer / Raj K. Puri / Bharat H. Joshi

    Journal of Translational Medicine, Vol 21, Iss 1, Pp 1-

    Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13Rα2 after radiolabeling with 89Zirconium oxine for PET imaging

    2023  Volume 2

    Keywords Medicine ; R
    Language English
    Publishing date 2023-09-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13Rα2 after radiolabeling with

    Leland, Pamela / Kumar, Dhiraj / Nimmagadda, Sridhar / Bauer, Steven R / Puri, Raj K / Joshi, Bharat H

    Journal of translational medicine

    2023  Volume 21, Issue 1, Page(s) 367

    Abstract: Background: Chimeric antigen receptor (CAR) T cell therapy is an exciting cell-based cancer immunotherapy. Unfortunately, CAR-T cell therapy is associated with serious toxicities such as cytokine release syndrome (CRS) and neurotoxicity. The mechanism ... ...

    Abstract Background: Chimeric antigen receptor (CAR) T cell therapy is an exciting cell-based cancer immunotherapy. Unfortunately, CAR-T cell therapy is associated with serious toxicities such as cytokine release syndrome (CRS) and neurotoxicity. The mechanism of these serious adverse events (SAEs) and how homing, distribution and retention of CAR-T cells contribute to toxicities is not fully understood. Enabling in vitro methods to allow meaningful, sensitive in vivo biodistribution studies is needed to better understand CAR-T cell disposition and its relationship to both effectiveness and safety of these products.
    Methods: To determine if radiolabelling of CAR-T cells could support positron emission tomography (PET)-based biodistribution studies, we labeled IL-13Rα2 targeting scFv-IL-13Rα2-CAR-T cells (CAR-T cells) with
    Results: We observed that radiolabeling of CAR-T cells with
    Conclusions: Importantly, radiolabeling has minimal impact on biological product attributes including potency of CAR-T cells towards IL-13Rα2 positive tumor cells but not IL-13Rα2 negative cells as measured by cytolytic activity and release of IFN-γ. Thus, IL-13Rα2 targeting CAR-T cells radiolabeled with
    MeSH term(s) Zirconium/pharmacokinetics ; Radioisotopes/pharmacokinetics ; Positron-Emission Tomography ; Cell Tracking/methods ; Immunotherapy, Adoptive ; Single-Chain Antibodies ; T-Lymphocytes/cytology ; Tissue Distribution ; Jurkat Cells ; Animals ; Mice ; Cell Proliferation ; Cell Survival
    Chemical Substances Zirconium (C6V6S92N3C) ; Radioisotopes ; Single-Chain Antibodies
    Language English
    Publishing date 2023-06-07
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2118570-0
    ISSN 1479-5876 ; 1479-5876
    ISSN (online) 1479-5876
    ISSN 1479-5876
    DOI 10.1186/s12967-023-04142-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Designer, Programmable DNA-peptide hybrid materials with emergent properties to probe and modulate biological systems.

    Menon, Dhruv / Singh, Ramesh / Joshi, Kashti B / Gupta, Sharad / Bhatia, Dhiraj

    Chembiochem : a European journal of chemical biology

    2023  Volume 24, Issue 5, Page(s) e202200580

    Abstract: The chemistry of DNA endows it with certain functional properties that facilitate the generation of self-assembled nanostructures, offering precise control over their geometry and morphology, that can be exploited for advanced biological applications. ... ...

    Abstract The chemistry of DNA endows it with certain functional properties that facilitate the generation of self-assembled nanostructures, offering precise control over their geometry and morphology, that can be exploited for advanced biological applications. Despite the structural promise of these materials, their applications are limited owing to lack of functional capability to interact favourably with biological systems, which has been achieved by functional proteins or peptides. Herein, we outline a strategy for functionalizing DNA structures with short-peptides, leading to the formation of DNA-peptide hybrid materials. This proposition offers the opportunity to leverage the unique advantages of each of these bio-molecules, that have far reaching emergent properties in terms of better cellular interactions and uptake, better stability in biological media, an acceptable and programmable immune response and high bioactive molecule loading capacities. We discuss the synthetic strategies for the formation of these materials, namely, solid-phase functionalization and solution-coupling functionalization. We then proceed to highlight selected biological applications of these materials in the domains of cell instruction & molecular recognition, gene delivery, drug delivery and bone & tissue regeneration. We conclude with discussions shedding light on the challenges that these materials pose and offer our insights on future directions of peptide-DNA research for targeted biomedical applications.
    MeSH term(s) DNA/chemistry ; Nanostructures/chemistry ; Drug Delivery Systems ; Peptides/chemistry ; Nanotechnology
    Chemical Substances DNA (9007-49-2) ; Peptides
    Language English
    Publishing date 2023-01-18
    Publishing country Germany
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202200580
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Silver-doped active carbon spheres and their application for microbial decontamination of water.

    Chandra Joshi, Harish / Dutta, Dhiraj / Gaur, Nisha / Singh, G S / Dubey, Rama / Dwivedi, S K

    Heliyon

    2022  Volume 8, Issue 4, Page(s) e09209

    Abstract: Highly efficient and durable, silver nanoparticles doped Active Carbon Spheres ACS(Ag) were synthesized by carbonization and activation of silver exchanged resins. The silver exchanged resins were prepared by exchanging ... ...

    Abstract Highly efficient and durable, silver nanoparticles doped Active Carbon Spheres ACS(Ag) were synthesized by carbonization and activation of silver exchanged resins. The silver exchanged resins were prepared by exchanging H
    Language English
    Publishing date 2022-03-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2022.e09209
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book ; Online: Contrastive Mean Teacher for Domain Adaptive Object Detectors

    Cao, Shengcao / Joshi, Dhiraj / Gui, Liang-Yan / Wang, Yu-Xiong

    2023  

    Abstract: Object detectors often suffer from the domain gap between training (source domain) and real-world applications (target domain). Mean-teacher self-training is a powerful paradigm in unsupervised domain adaptation for object detection, but it struggles ... ...

    Abstract Object detectors often suffer from the domain gap between training (source domain) and real-world applications (target domain). Mean-teacher self-training is a powerful paradigm in unsupervised domain adaptation for object detection, but it struggles with low-quality pseudo-labels. In this work, we identify the intriguing alignment and synergy between mean-teacher self-training and contrastive learning. Motivated by this, we propose Contrastive Mean Teacher (CMT) -- a unified, general-purpose framework with the two paradigms naturally integrated to maximize beneficial learning signals. Instead of using pseudo-labels solely for final predictions, our strategy extracts object-level features using pseudo-labels and optimizes them via contrastive learning, without requiring labels in the target domain. When combined with recent mean-teacher self-training methods, CMT leads to new state-of-the-art target-domain performance: 51.9% mAP on Foggy Cityscapes, outperforming the previously best by 2.1% mAP. Notably, CMT can stabilize performance and provide more significant gains as pseudo-label noise increases.

    Comment: CVPR 2023
    Keywords Computer Science - Computer Vision and Pattern Recognition
    Subject code 006
    Publishing date 2023-05-04
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13Rα2 after radiolabeling with 89Zirconium oxine for PET imaging

    Pamela Leland / Dhiraj Kumar / Sridhar Nimmagadda / Steven R. Bauer / Raj K. Puri / Bharat H. Joshi

    Journal of Translational Medicine, Vol 21, Iss 1, Pp 1-

    2023  Volume 14

    Abstract: Abstract Background Chimeric antigen receptor (CAR) T cell therapy is an exciting cell-based cancer immunotherapy. Unfortunately, CAR-T cell therapy is associated with serious toxicities such as cytokine release syndrome (CRS) and neurotoxicity. The ... ...

    Abstract Abstract Background Chimeric antigen receptor (CAR) T cell therapy is an exciting cell-based cancer immunotherapy. Unfortunately, CAR-T cell therapy is associated with serious toxicities such as cytokine release syndrome (CRS) and neurotoxicity. The mechanism of these serious adverse events (SAEs) and how homing, distribution and retention of CAR-T cells contribute to toxicities is not fully understood. Enabling in vitro methods to allow meaningful, sensitive in vivo biodistribution studies is needed to better understand CAR-T cell disposition and its relationship to both effectiveness and safety of these products. Methods To determine if radiolabelling of CAR-T cells could support positron emission tomography (PET)-based biodistribution studies, we labeled IL-13Rα2 targeting scFv-IL-13Rα2-CAR-T cells (CAR-T cells) with 89Zirconium-oxine (89Zr-oxine) and characterized and compared their product attributes with non-labeled CAR-T cells. The 89Zr-oxine labeling conditions were optimized for incubation time, temperature, and use of serum for labeling. In addition, T cell subtype characterization and product attributes of radiolabeled CAR-T cells were studied to assess their overall quality including cell viability, proliferation, phenotype markers of T-cell activation and exhaustion, cytolytic activity and release of interferon-γ upon co-culture with IL-13Rα2 expressing glioma cells. Results We observed that radiolabeling of CAR-T cells with 89Zr-oxine is quick, efficient, and radioactivity is retained in the cells for at least 8 days with minimal loss. Also, viability of radiolabeled CAR-T cells and subtypes such as CD4 + , CD8 + and scFV-IL-13Rα2 transgene positive T cell population were characterized and found similar to that of unlabeled cells as determined by TUNEL assay, caspase 3/7 enzyme and granzyme B activity assay. Moreover, there were no significant changes in T cell activation (CD24, CD44, CD69 and IFN-γ) or T cell exhaustion (PD-1, LAG-3 and TIM3) markers expression between radiolabeled and unlabeled ...
    Keywords CAR-T Cells ; 89Zr ; Radioactivity ; PET ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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