LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU=Sakaguchi Atsumi
  2. AU="Rodriguez-Cabello, Jose C"
  3. AU=Merter Ayse Arducoglu
  4. AU="Ewers, U."
  5. AU="Reichling, Jürgen"
  6. AU="Eichner, Timo"
  7. AU="Seunghee Kim-Schulze"
  8. AU=Ozaki Shoichi
  9. AU="Clerici, Giovanna" AU="Clerici, Giovanna"
  10. AU="Firoz Ahmed"
  11. AU="Dżugan, Małgorzata"
  12. AU="Rolf Bjerkvig"
  13. AU=Khosravi-Far Roya
  14. AU="Udeochu, Joe C"
  15. AU="Osoba, Osonde A." AU="Osoba, Osonde A."
  16. AU="Palani, Sowmiya"
  17. AU="Manfred Frick"
  18. AU="Jensen, Leonard"
  19. AU="Pakhomov, Evgeny A."
  20. AU="Subramanyam, Chithirala Bala"
  21. AU=Petrek J A
  22. AU="Thomson-Baker, B"
  23. AU=Shahidi Shahrzad
  24. AU="Taylor, Holly A"
  25. AU="Yeong Jeong Jeon"
  26. AU="Bueno-Cavanillas, Aurora"
  27. AU="Kavčič, Tina"
  28. AU="Arias-Jiménez, José Luís"
  29. AU="Tünçok, Ekin"
  30. AU="Roberto Toro"
  31. AU="Bharti Sahu"
  32. AU="Soo-Yeon Choi"
  33. AU="Nono, Sandra"
  34. AU="Diepens, Robin J W"
  35. AU="Baselga-Garriga, Clara"

Suchergebnis

Treffer 1 - 6 von insgesamt 6

Suchoptionen

  1. Artikel ; Online: Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

    Atsumi Sakaguchi / Yoichiro Tanaka / Eiki Shoji / Teppei Takeshima / Rina Sakamaki / Takao Matsuba / Yasuyuki Kurihara

    Journal of Biological Engineering, Vol 17, Iss 1, Pp 1-

    2023  Band 15

    Abstract: Highlights ・MIHS-SAST screening generates mAbs that target protein structures. ・One-fourth of the mAbs recognized stereospecific epitopes of the protein. ・Two fluorometric parameters aided in selecting antibodies with strong binding constants. ...

    Abstract Highlights ・MIHS-SAST screening generates mAbs that target protein structures. ・One-fourth of the mAbs recognized stereospecific epitopes of the protein. ・Two fluorometric parameters aided in selecting antibodies with strong binding constants.
    Schlagwörter Monoclonal antibody ; Flow cytometer ; Conformation-specific antibody ; ELISA ; Hybridoma technology ; Biology (General) ; QH301-705.5
    Sprache Englisch
    Erscheinungsdatum 2023-03-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  2. Artikel: Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology.

    Sakaguchi, Atsumi / Tanaka, Yoichiro / Shoji, Eiki / Takeshima, Teppei / Sakamaki, Rina / Matsuba, Takao / Kurihara, Yasuyuki

    Journal of biological engineering

    2023  Band 17, Heft 1, Seite(n) 24

    Abstract: Background: Monoclonal antibodies are essential in life science research and developing antibody drugs and test drugs. Various methods have been developed to obtain monoclonal antibodies, among which hybridoma technology continues to be widely used. ... ...

    Abstract Background: Monoclonal antibodies are essential in life science research and developing antibody drugs and test drugs. Various methods have been developed to obtain monoclonal antibodies, among which hybridoma technology continues to be widely used. However, developing a rapid and efficient method for obtaining conformation-specific antibodies using hybridoma technology remains challenging. We previously developed the membrane-type immunoglobulin-directed hybridoma screening (MIHS) method, which is a flow cytometry-based screening technique based on the interaction between the B-cell receptor expressed on the hybridoma cell surface and the antigen protein, to obtain conformation-specific antibodies.
    Results: In this study, we proposed a streptavidin-anchored ELISA screening technology (SAST) as a secondary screening method that retains the advantages of the MIHS method. Anti-enhanced green fluorescent protein monoclonal antibodies were generated as a model experiment, and their structural recognition abilities were examined. Examination of the reaction profiles showed that all monoclonal antibodies obtained in this study recognize the conformational epitopes of the protein antigen. Furthermore, these monoclonal antibodies were classified into two groups: those with binding activities against partially denatured proteins and those with complete loss of binding activities. Next, when screening monoclonal antibodies by the MIHS method as the first screening, we found that monoclonal antibodies with stronger binding constants may be selected by double-staining for hybridomas with fluorescently labeled target antigens and fluorescently labeled B cell receptor antibodies.
    Conclusions: The proposed two-step screening method, which incorporates MIHS and SAST, constitutes a rapid, simple, and effective strategy to obtain conformation-specific monoclonal antibodies generated through hybridoma technology. The novel monoclonal antibody screening strategy reported herein could accelerate the development of antibody drugs and antibody tests.
    Sprache Englisch
    Erscheinungsdatum 2023-03-30
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2391582-1
    ISSN 1754-1611
    ISSN 1754-1611
    DOI 10.1186/s13036-023-00345-9
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monoclonal antibody.

    Sakaguchi, Atsumi / Nakajima, Chika / Sawano, Ayuko / Tanaka, Yoichiro / Kurihara, Yasuyuki

    Journal of bioscience and bioengineering

    2021  Band 131, Heft 6, Seite(n) 696–702

    Abstract: Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as ... ...

    Abstract Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 virus. This study has developed a membrane-type immunoglobulin-directed hybridoma screening (MIHS) method for obtaining high-quality monoclonal antibodies with high efficiency and high speed. In addition to these advantages, this paper demonstrates that the MIHS method can selectively obtain monoclonal antibodies that specifically recognize the functional structure of proteins. The MIHS method is a useful technology that greatly contributes to the research community because it can be easily introduced in any laboratory that uses a flow cytometer.
    Mesh-Begriff(e) Animals ; Antibodies, Monoclonal/analysis ; Antibodies, Monoclonal/biosynthesis ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/isolation & purification ; Antibodies, Neutralizing/analysis ; Antibodies, Neutralizing/biosynthesis ; Antibodies, Neutralizing/immunology ; Antibodies, Neutralizing/isolation & purification ; Antibody Specificity/immunology ; Cell Line, Tumor ; Enzyme-Linked Immunosorbent Assay/methods ; Flow Cytometry/methods ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/immunology ; Green Fluorescent Proteins/metabolism ; Humans ; Hybridomas/cytology ; Hybridomas/metabolism ; Immunoglobulin Isotypes ; Immunoprecipitation ; Mice ; Time Factors
    Chemische Substanzen Antibodies, Monoclonal ; Antibodies, Neutralizing ; Immunoglobulin Isotypes ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9)
    Sprache Englisch
    Erscheinungsdatum 2021-03-19
    Erscheinungsland Japan
    Dokumenttyp Journal Article
    ZDB-ID 1465387-4
    ISSN 1347-4421 ; 1389-1723
    ISSN (online) 1347-4421
    ISSN 1389-1723
    DOI 10.1016/j.jbiosc.2021.02.006
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Bonn

    Z 4728: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  4. Artikel ; Online: Coxfa4l3, a novel mitochondrial electron transport chain Complex 4 subunit protein, switches from Coxfa4 during spermatogenesis.

    Endou, Masahiro / Yoshida, Kaito / Hirota, Makoto / Nakajima, Chika / Sakaguchi, Atsumi / Komatsubara, Naoto / Kurihara, Yasuyuki

    Mitochondrion

    2020  Band 52, Seite(n) 1–7

    Abstract: We identified Coxfa4l3, previously called C15orf48 or Nmes1, as a novel accessory protein of Complex IV of the mitochondrial electron transport chain (ETC). Amino acid sequence comparison, the intracellular localization and the protein expression data ... ...

    Abstract We identified Coxfa4l3, previously called C15orf48 or Nmes1, as a novel accessory protein of Complex IV of the mitochondrial electron transport chain (ETC). Amino acid sequence comparison, the intracellular localization and the protein expression data showed that the protein is the third isoform of Coxfa4 and the expression of Coxfa4 and Coxfa4l3 proteins during spermatogenesis showed a mutually exclusive pattern, implying that Coxfa4 replaces Coxfa4l3 in Complex IV after meiosis. These results may provide some insight into the unique mechanism of ATP production in late spermatogenesis.
    Mesh-Begriff(e) Animals ; Cell Line ; Electron Transport Complex IV/genetics ; Electron Transport Complex IV/metabolism ; HEK293 Cells ; HeLa Cells ; Humans ; Male ; Mice ; Nuclear Proteins/metabolism ; Organ Specificity ; Proteomics ; Spermatogenesis
    Chemische Substanzen Nuclear Proteins ; Electron Transport Complex IV (EC 1.9.3.1)
    Sprache Englisch
    Erscheinungsdatum 2020-02-08
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 2056923-3
    ISSN 1872-8278 ; 1567-7249
    ISSN (online) 1872-8278
    ISSN 1567-7249
    DOI 10.1016/j.mito.2020.02.003
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel ; Online: Ultrasound-guided peripheral intravenous access placement for children in the emergency department.

    Otani, Takehito / Morikawa, Yoshihiko / Hayakawa, Itaru / Atsumi, Yukari / Tomari, Kouki / Tomobe, Yutaro / Uda, Kazuhiro / Funakoshi, Yu / Sakaguchi, Chiho / Nishimoto, Shizuka / Hataya, Hiroshi

    European journal of pediatrics

    2018  Band 177, Heft 10, Seite(n) 1443–1449

    Abstract: The usefulness of ultrasound guidance in peripheral intravenous access placement has yet to be established in children. In this prospective comparative study, we investigated success rates of intravenous access placement with ultrasound guidance in a ... ...

    Abstract The usefulness of ultrasound guidance in peripheral intravenous access placement has yet to be established in children. In this prospective comparative study, we investigated success rates of intravenous access placement with ultrasound guidance in a pediatric emergency department. After a failed first attempt with the conventional technique, the second and third attempts were conducted using either the ultrasound guidance (a real-time, dual operator method) or the conventional technique. The success rates within the two interventional attempts were then compared. From a total of 712 participants, those with a failed first attempt were allocated to the ultrasound guidance (n = 99) and conventional technique (n = 100) groups. The success rate was significantly lower for the ultrasound guidance (65%) than for the conventional technique (84%) group (p = 0.002, chi-square test). This remained significant after adjusting for confounders with multiple logistic regression analysis (odds ratio 2.60, 95% confidence interval 1.26-5.37, p = 0.001).
    Conclusion: Ultrasound-guided intravenous access placement using a real-time, dual operator method led to a significantly lower success rate than the conventional technique in children with one failed conventional attempt in the emergency department.
    Trial registration: UMIN000014730 What is Known: • Children experience a low success rate (about 60% with 1 attempt and about 90% with 4 attempts) for IV access placement. • Ultrasound guidance may lead to a decreased number of attempts and shorter procedural time with comparable overall IV success rate. What is New: • Ultrasound-guided IV placement (a real-time, dual operator method) actually led to a significantly lower IV success rate than the conventional technique in children in the emergency department. • Our result warrants further trials to determine the precise population who benefits from ultrasound guidance.
    Mesh-Begriff(e) Catheterization, Peripheral/adverse effects ; Catheterization, Peripheral/methods ; Child ; Child, Preschool ; Emergency Service, Hospital/statistics & numerical data ; Female ; Humans ; Infant ; Infusions, Intravenous ; Male ; Prospective Studies ; Ultrasonography, Interventional/adverse effects ; Ultrasonography, Interventional/methods
    Sprache Englisch
    Erscheinungsdatum 2018-06-30
    Erscheinungsland Germany
    Dokumenttyp Clinical Trial ; Comparative Study ; Journal Article
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-018-3201-3
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Um IV Zs.75: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  6. Artikel: Effect of Osteocyte-Ablation on Inorganic Phosphate Metabolism: Analysis of Bone-Kidney-Gut Axis.

    Fujii, Osamu / Tatsumi, Sawako / Ogata, Mao / Arakaki, Tomohiro / Sakaguchi, Haruna / Nomura, Kengo / Miyagawa, Atsumi / Ikuta, Kayo / Hanazaki, Ai / Kaneko, Ichiro / Segawa, Hiroko / Miyamoto, Ken-Ichi

    Frontiers in endocrinology

    2017  Band 8, Seite(n) 359

    Abstract: In response to kidney damage, osteocytes increase the production of several hormones critically involved in mineral metabolism. Recent studies suggest that osteocyte function is altered very early in the course of chronic kidney disease. In the present ... ...

    Abstract In response to kidney damage, osteocytes increase the production of several hormones critically involved in mineral metabolism. Recent studies suggest that osteocyte function is altered very early in the course of chronic kidney disease. In the present study, to clarify the role of osteocytes and the canalicular network in mineral homeostasis, we performed four experiments. In Experiment 1, we investigated renal and intestinal Pi handling in osteocyte-less (OCL) model mice [transgenic mice with the dentin matrix protein-1 promoter-driven diphtheria toxin (DT)-receptor that were injected with DT]. In Experiment 2, we administered granulocyte colony-stimulating factor to mice to disrupt the osteocyte canalicular network. In Experiment 3, we investigated the role of osteocytes in dietary Pi signaling. In Experiment 4, we analyzed gene expression level fluctuations in the intestine and liver by comparing mice fed a high Pi diet and OCL mice. Together, the findings of these experiments indicate that osteocyte ablation caused rapid renal Pi excretion (
    Sprache Englisch
    Erscheinungsdatum 2017
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2017.00359
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang