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  1. Article ; Online: Long-Term Efficacy of Radio Frequency Ablation for Superficial Lesions of the Tracheobronchial Tree.

    Steppert, Claus / Linsmeier, Bernd

    Annals of the American Thoracic Society

    2021  Volume 18, Issue 2, Page(s) 353–355

    MeSH term(s) Carcinoma, Renal Cell ; Catheter Ablation ; Humans ; Kidney Neoplasms ; Radiofrequency Ablation
    Language English
    Publishing date 2021-02-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2717461-X
    ISSN 2325-6621 ; 1943-5665 ; 2325-6621
    ISSN (online) 2325-6621 ; 1943-5665
    ISSN 2325-6621
    DOI 10.1513/AnnalsATS.202002-127CC
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  2. Article ; Online: Rapid detection of SARS-CoV-2 infection by multicapillary column coupled ion mobility spectrometry (MCC-IMS) of breath. A proof of concept study.

    Steppert, Claus / Steppert, Isabel / Sterlacci, William / Bollinger, Thomas

    Journal of breath research

    2021  Volume 15, Issue 2

    Abstract: There is an urgent need for screening of patients with a communicable viral disease to cut infection chains. Recently, we demonstrated that ion mobility spectrometry coupled with a multicapillary column (MCC-IMS) is able to identify influenza-A ... ...

    Abstract There is an urgent need for screening of patients with a communicable viral disease to cut infection chains. Recently, we demonstrated that ion mobility spectrometry coupled with a multicapillary column (MCC-IMS) is able to identify influenza-A infections in patients' breath. With a decreasing influenza epidemic and upcoming SARS-CoV-2 infections we proceeded further and analyzed patients with suspected SARS-CoV-2 infections. In this study, the nasal breath of 75 patients (34 male, 41 female, aged 64.4 ± 15.4 years) was investigated by MCC-IMS for viral infections. Fourteen were positively diagnosed with influenza-A infection and sixteen with SARS-CoV-2 by reverse transcription polymerase chain reaction (RT-PCR) of nasopharyngeal swabs. In one patient RT-PCR was highly suspicious of SARS-CoV-2 but initially inconclusive. The remaining 44 patients served as controls. Breath fingerprints for specific infections were assessed by a combination of cluster analysis and multivariate statistics. There were no significant differences in gender or age according to the groups. In the cross validation of the discriminant analysis 72 of the 74 clearly defined patients could be correctly classified to the respective group. Even the inconclusive patient could be mapped to the SARS-CoV-2 group by applying the discrimination functions. Conclusion: SARS-CoV-2 infection and influenza-A infection can be detected with the help of MCC-IMS in breath in this pilot study. As this method provides a fast non-invasive diagnosis it should be further developed in a larger cohort for screening of communicable viral diseases. A validation study is ongoing during the second wave of COVID-19.Trial registration: ClinicalTrial.gov, NCT04282135 Registered 20 February 2020-Retrospectively registered,https://clinicaltrials.gov/ct2/show/NCT04282135?term=IMS&draw=2&rank=1.
    MeSH term(s) Aged ; Breath Tests ; COVID-19 ; Female ; Humans ; Ion Mobility Spectrometry ; Male ; Middle Aged ; Pilot Projects ; Proof of Concept Study ; SARS-CoV-2
    Language English
    Publishing date 2021-03-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2381007-5
    ISSN 1752-7163 ; 1752-7155
    ISSN (online) 1752-7163
    ISSN 1752-7155
    DOI 10.1088/1752-7163/abe5ca
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  3. Article ; Online: Rapid non-invasive detection of Influenza-A-infection by multicapillary column coupled ion mobility spectrometry.

    Steppert, Claus / Steppert, Isabel / Bollinger, Thomas / Sterlacci, William

    Journal of breath research

    2020  Volume 15, Issue 1, Page(s) 11001

    Abstract: Infectious pathogens are a global issue. Global air travel offers an easy and fast opportunity not only for people but also for infectious diseases to spread around the world within a few days. Also, large public events facilitate increasing infection ... ...

    Abstract Infectious pathogens are a global issue. Global air travel offers an easy and fast opportunity not only for people but also for infectious diseases to spread around the world within a few days. Also, large public events facilitate increasing infection numbers. Therefore, rapid on-site screening for infected people is urgently needed. Due to the small size and easy handling, ion mobility spectrometry coupled with a multicapillary column (MCC-IMS) is a very promising, sensitive method for the on-site identification of infectious pathogens based on scents, representing volatile organic compounds (VOCs). The purpose of this study was to prospectively assess whether identification of Influenza-A-infection based on VOCs by MCC-IMS is possible in breath. Nasal breath was investigated in 24 consecutive persons with and without Influenza-A-infection by MCC-IMS. In 14 Influenza-A-infected patients, infection was proven by PCR of nasopharyngeal swabs. Four healthy staff members and six patients with negative PCR result served as controls. For picking up relevant VOCs in MCC-IMS spectra, software based on cluster analysis followed by multivariate statistical analysis was applied. With only four VOCs canonical discriminant analysis was able to distinguish Influenza-A-infected patients from those not infected with 100% sensitivity and 100% specificity. This present proof-of-concept-study yields encouraging results showing a rapid diagnosis of viral infections in nasal breath within 5 min by MCC-IMS. The next step is to validate the results with a greater number of patients with Influenza-A-infection as well as other viral diseases, especially COVID-19. Registration number at ClinicalTrials.gov NCT04282135.
    MeSH term(s) Aged ; Betacoronavirus/isolation & purification ; Breath Tests ; COVID-19 ; Coronavirus Infections/complications ; Coronavirus Infections/diagnosis ; Discriminant Analysis ; Female ; Humans ; Influenza A virus/isolation & purification ; Influenza, Human/complications ; Influenza, Human/diagnosis ; Ion Mobility Spectrometry ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/diagnosis ; Prospective Studies ; SARS-CoV-2 ; Sensitivity and Specificity
    Keywords covid19
    Language English
    Publishing date 2020-10-22
    Publishing country England
    Document type Evaluation Study ; Journal Article
    ZDB-ID 2381007-5
    ISSN 1752-7163 ; 1752-7155
    ISSN (online) 1752-7163
    ISSN 1752-7155
    DOI 10.1088/1752-7163/abb762
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  4. Book ; Thesis: Wertigkeit der pharmakologisch induzierten Autonomen Blockade in der Analyse der gestörten Sinusknotenfunktion

    Steppert, Claus

    1985  

    Author's details vorgelegt von Claus Steppert
    Size 85 S. : zahlr. graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Mainz, Univ., Diss., 1985
    HBZ-ID HT002835726
    Database Catalogue ZB MED Medicine, Health

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  5. Article: Rapid non-invasive detection of Influenza-A-infection by multicapillary column coupled ion mobility spectrometry

    Steppert, Claus / Steppert, Isabel / Bollinger, Thomas / Sterlacci, William

    J Breath Res

    Abstract: Infectious pathogens are a global issue. Global air travel offers an easy and fast opportunity not only for people but also for infectious diseases to spread around the world within a few days. Also, large public events facilitate increasing infection ... ...

    Abstract Infectious pathogens are a global issue. Global air travel offers an easy and fast opportunity not only for people but also for infectious diseases to spread around the world within a few days. Also, large public events facilitate increasing infection numbers. Therefore, rapid on-site screening for infected people is urgently needed. Due to the small size and easy handling, ion mobility spectrometry coupled with a multicapillary column (MCC-IMS) is a very promising, sensitive method for the on-site identification of infectious pathogens based on scents, representing volatile organic compounds (VOCs). The purpose of this study was to prospectively assess whether identification of Influenza-A-infection based on VOCs by MCC-IMS is possible in breath. Nasal breath was investigated in 24 consecutive persons with and without Influenza-A-infection by MCC-IMS. In 14 Influenza-A-infected patients, infection was proven by PCR of nasopharyngeal swabs. Four healthy staff members and six patients with negative PCR result served as controls. For picking up relevant VOCs in MCC-IMS spectra, software based on cluster analysis followed by multivariate statistical analysis was applied. With only four VOCs canonical discriminant analysis was able to distinguish Influenza-A-infected patients from those not infected with 100% sensitivity and 100% specificity. This present proof-of-concept-study yields encouraging results showing a rapid diagnosis of viral infections in nasal breath within 5 min by MCC-IMS. The next step is to validate the results with a greater number of patients with Influenza-A-infection as well as other viral diseases, especially COVID-19. Registration number at ClinicalTrials.gov NCT04282135.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #889456
    Database COVID19

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  6. Article ; Online: Detection of SARS-CoV-2 Infection by Multicapillary Column Coupled Ion Mobility Spectrometry (MCC-IMS) of Breath

    Steppert, Claus / Steppert, Isabel / Becher, Gunther / Sterlacci, William / Bollinger, Thomas

    SSRN Electronic Journal ; ISSN 1556-5068

    A Proof of Concept Study

    2020  

    Keywords covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    DOI 10.2139/ssrn.3633195
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Simultaneous endocrine expression and loss of melanoma markers in malignant melanoma metastases, a retrospective analysis.

    Steppert, Claus / Krugmann, Jens / Sterlacci, William

    Pathology oncology research : POR

    2019  Volume 26, Issue 3, Page(s) 1777–1779

    Abstract: Malignant melanoma metastases are chameleons of histopathology. In 4 primary malignant melanomas and 20 melanoma metastases expression of S-100, HMB-45 and melan-A as melanoma markers and CD56, synaptophysin and chromogranin-A as neuroendocrine markers ... ...

    Abstract Malignant melanoma metastases are chameleons of histopathology. In 4 primary malignant melanomas and 20 melanoma metastases expression of S-100, HMB-45 and melan-A as melanoma markers and CD56, synaptophysin and chromogranin-A as neuroendocrine markers was retrospectively analyzed. While all primary tumors expressed all 3 melanoma markers 7/20 of melanoma metastases had lost at least one melanoma marker, one had lost all three markers. Conversely about half of the samples stained for CD56, only 6/20 metastases were negative for all 3 neuroendocrine markers. None expressed chromogranin-A. Partial loss of melanoma markers and expression of neuroendocrine markers seems not to be infrequent. In patients with a history of malignant melanoma and suspected metastases, losing melanoma markers while expressing neuroendocrine markers is a potential diagnostic pitfall. Therefore all 3 melanoma markers should be performed as well as chromogranin-A staining. In doubt, metastases of the melanoma should be assumed.
    MeSH term(s) Aged ; Aged, 80 and over ; Biomarkers, Tumor/analysis ; Female ; Humans ; Male ; Melanoma/diagnosis ; Melanoma/pathology ; Middle Aged ; Neoplasm Metastasis/diagnosis ; Neoplasm Metastasis/pathology ; Retrospective Studies ; Skin Neoplasms/diagnosis ; Skin Neoplasms/pathology ; Melanoma, Cutaneous Malignant
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2019-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1375979-6
    ISSN 1532-2807 ; 1219-4956
    ISSN (online) 1532-2807
    ISSN 1219-4956
    DOI 10.1007/s12253-019-00761-7
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    Zs.A 4936: Show issues Location:
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  8. Article: Spiroergometrie in der erweiterten flugmedizinischen kardiovaskulären Beurteilung

    Steppert, Claus / Stumpf, Christian

    Flugmedizin · Tropenmedizin · Reisemedizin - FTR

    2019  Volume 26, Issue 04, Page(s) 160–164

    Abstract: Die EU-Verordnung 1178/2011 fordert in speziellen Fällen eine erweiterte kardiovaskuläre Beurteilung, der Umfang ist jedoch nicht spezifiziert. Neben Ergometrie, Echokardiografie und Doppler der Halsgefäße werden vom Luftfahrtbundesamt zahlreiche ... ...

    Abstract Die EU-Verordnung 1178/2011 fordert in speziellen Fällen eine erweiterte kardiovaskuläre Beurteilung, der Umfang ist jedoch nicht spezifiziert. Neben Ergometrie, Echokardiografie und Doppler der Halsgefäße werden vom Luftfahrtbundesamt zahlreiche Laborparameter gefordert. In den Leitlinien zum Management der stabilen koronaren Herzkrankheit ist die Ergometrie weitgehend obsolet. Stress-Echokardiografie und Stress- MRT bieten eine bessere diagnostische und prognostische Beurteilung. Die Spiroergometrie kombiniert die Ergometrie mit einer Messung respiratorischer Parameter. Hierüber kann das Herzzeitvolumen abgeschätzt und ischämische Veränderungen detektiert werden. Die Wertigkeit in der Erkennung ischämischer Veränderungen entspricht der des Stress-MRT bei signifikant niedrigeren Kosten. Bei einer Spiroergometrie ohne Ischämiezeichen ist die negative prädiktive Wertigkeit sehr hoch. Wir empfehlen die Spiroergometerie deshalb als Ersatz teurer prognostischer Untersuchungen.
    Keywords cardiopulmonary exercise testing ; prognostic ; ischemic disturbances ; lower cost
    Language German
    Publishing date 2019-08-01
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 2277004-5
    ISSN 1864-175X ; 1864-4538
    ISSN (online) 1864-175X
    ISSN 1864-4538
    DOI 10.1055/a-0967-4178
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  9. Article ; Online: Rapid detection of SARS-CoV-2 infection by multicapillary column coupled ion mobility spectrometry (MCC-IMS) of breath. A proof of concept study

    Steppert, Claus / Steppert, Isabel / Becher, Gunther / Sterlacci, William / Bollinger, Thomas

    medRxiv

    Abstract: There is an urgent need for screening patients of having a communicable viral disease to cut infection chains. We could recently demonstrate that MCC-IMS of breath is able to identify Influenza-A infected patients. With decreasing Influenza epidemic and ... ...

    Abstract There is an urgent need for screening patients of having a communicable viral disease to cut infection chains. We could recently demonstrate that MCC-IMS of breath is able to identify Influenza-A infected patients. With decreasing Influenza epidemic and upcoming SARS-CoV-2 infections we extended our study to the analysis of patients with suspected SARS-CoV-2 infections. 51 patients, 23m, 28f, aged 64 +/- 16 years, were included in this study. Besides RT-PCR analysis of nasopharyngeal swabs all patients underwent MCC-IMS analysis of breath. 16 patients, 7m, 9f, were positive for SARS-CoV-2 by RT-PCR. There was no difference in gender or age according to the groups. Stepwise canonical discriminant analysis could correctly classify the infected and non- infected subjects in 98% by cross-validation. Afterwards we combined the Influenza-A sub study and the SARS-CoV-2- sub study for a total of 75 patients, 34m, 41f, aged 64.8 +/- 1.8 years, 14 positive for Influenza-A, 16 positive for SARS-CoV-2, the remaining 44 patients were used as controls. In one patient RT-PCR was highly suspicious of SARS-CoV-2 but inconclusive. There was no imbalance between the groups for age or gender. 97.3% of the patients could be correctly classified to the respective group by discriminant analysis. Even the inconclusive patient could be mapped to the SARS-CoV-2 group applying the discrimination function. Conclusion: MCC-IMS is able to detect SARS-CoV-2 infection and Influenza-A infection in breath. As this method provides exact, fast non-invasive diagnosis it should be further developed for screening of communicable viral diseases. Study registration: NCT04282135
    Keywords covid19
    Language English
    Publishing date 2020-07-03
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.06.30.20143347
    Database COVID19

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  10. Article: Ability of

    Zhang, Ruiyun / Shi, Kuangyu / Hohenforst-Schmidt, Wolfgang / Steppert, Claus / Sziklavari, Zsolt / Schmidkonz, Christian / Atzinger, Armin / Hartmann, Arndt / Vieth, Michael / Förster, Stefan

    Cancers

    2023  Volume 15, Issue 14

    Abstract: Objective: Considering the essential role of KRAS mutation in NSCLC and the limited experience of PET radiomic features in KRAS mutation, a prediction model was built in our current analysis. Our model aims to evaluate the status of KRAS mutants in lung ...

    Abstract Objective: Considering the essential role of KRAS mutation in NSCLC and the limited experience of PET radiomic features in KRAS mutation, a prediction model was built in our current analysis. Our model aims to evaluate the status of KRAS mutants in lung adenocarcinoma by combining PET radiomics and machine learning.
    Method: Patients were retrospectively selected from our database and screened from the NSCLC radiogenomic dataset from TCIA. The dataset was randomly divided into three subgroups. Two open-source software programs, 3D Slicer and Python, were used to segment lung tumours and extract radiomic features from
    Results: One hundred and nineteen patients with lung adenocarcinoma were included in our study. The whole group was divided into three datasets: a training set (
    Conclusions: Our model combining
    Language English
    Publishing date 2023-07-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers15143684
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