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Article ; Online: Standalone cell culture microfluidic device-based microphysiological system for automated cell observation and application in nephrotoxicity tests.

Kimura, Hiroshi / Nakamura, Hiroko / Goto, Tomomi / Uchida, Wakana / Uozumi, Takayuki / Nishizawa, Daniel / Shinha, Kenta / Sakagami, Junko / Doi, Kotaro

Lab on a chip

2024 Volume 24, Issue 3, Page(s) 408–421

Abstract: Microphysiological systems (MPS) offer an alternative method for culturing cells on microfluidic platforms to model organ functions in pharmaceutical and medical sciences. Although MPS hardware has been proposed to maintain physiological organ function ... ...

Abstract	Microphysiological systems (MPS) offer an alternative method for culturing cells on microfluidic platforms to model organ functions in pharmaceutical and medical sciences. Although MPS hardware has been proposed to maintain physiological organ function through perfusion culture, no existing MPS can automatically assess cell morphology and conditions online to observe cellular dynamics in detail. Thus, with this study, we aimed to establish a practical strategy for automating cell observation and improving cell evaluation functions with low temporal resolution and throughput in MPS experiments. We developed a versatile standalone cell culture microfluidic device (SCCMD) that integrates microfluidic chips and their peripherals. This device is compliant with the ANSI/SLAS standards and has been seamlessly integrated into an existing automatic cell imaging system. This integration enables automatic cell observation with high temporal resolution in MPS experiments. Perfusion culture of human kidney proximal tubule epithelial cells using the SCCMD improves cell function. By combining the proximal tubule MPS with an existing cell imaging system, nephrotoxicity studies were successfully performed to automate morphological and material permeability evaluation. We believe that the concept of building the ANSI/SLAS-compliant-sized MPS device proposed herein and integrating it into an existing automatic cell imaging system for the online measurement of detailed cell dynamics information and improvement of throughput by automating observation operations is a novel potential research direction for MPS research.
MeSH term(s)	Humans ; Microphysiological Systems ; Cell Culture Techniques ; Lab-On-A-Chip Devices ; Microfluidics/methods ; Epithelial Cells
Language	English
Publishing date	2024-01-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2056646-3
ISSN	1473-0189 ; 1473-0197
ISSN (online)	1473-0189
ISSN	1473-0197
DOI	10.1039/d3lc00934c
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Reversible suspension culture of human vascular smooth muscle cells using the functional biopolymer FP003.

Uchida, Masashi / Higashimoto, Rina / Hoshino, Takumi / Natori, Tomomi / Hatanaka, Daisuke / Hayashi, Hisato / Kanaki, Tatsuro / Suzuki, Takaaki / Ishii, Itsuko

In vitro cellular & developmental biology. Animal

2023 Volume 59, Issue 7, Page(s) 505–513

Abstract: Human vascular smooth muscle cells (SMCs) are adherent cells, and they cannot survive without scaffolds in suspension culture. Here, we aimed to establish a suspension culture of SMCs using the functional biopolymer FP003 and to investigate the ... ...

Abstract	Human vascular smooth muscle cells (SMCs) are adherent cells, and they cannot survive without scaffolds in suspension culture. Here, we aimed to establish a suspension culture of SMCs using the functional biopolymer FP003 and to investigate the proliferation status of the cells. When SMCs were suspension cultured with FP003, their proliferation was inhibited with a viability of 75% until day 15. When SMCs were re-plated on plastic plates after suspension culture with FP003 for 48 h, the SMCs proliferated as in a normal plate culture. The SMCs cultured in suspension with FP003 showed a relatively low phosphorylation of retinoblastoma protein, low expression of cyclin D1, high proportion of G0/G1 phase cells, low proportion of S phase cells, and no obvious signs of apoptosis, indicating that this culture system inhibited progression from the G1 to S phase. This growth arrest was a reversible property that showed no significant changes in the expressions of the marker proteins α-smooth muscle actin and smooth muscle myosin heavy chain. These results suggest that human SMCs can be stably cultured in suspension with FP003 without losing their characteristics when they are cultured on plastic plates again.
MeSH term(s)	Humans ; Animals ; Cells, Cultured ; Muscle, Smooth, Vascular ; Proteins/metabolism ; Biopolymers/metabolism ; Myocytes, Smooth Muscle/metabolism
Chemical Substances	FP-025 ; Proteins ; Biopolymers
Language	English
Publishing date	2023-07-28
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1077810-x
ISSN	1543-706X ; 0883-8364 ; 1071-2690
ISSN (online)	1543-706X
ISSN	0883-8364 ; 1071-2690
DOI	10.1007/s11626-023-00788-w
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Article ; Online: The Effect of Cysteine Peptide Ingestion on Skin Brightness, a Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Human Clinical Trial

Yoshiaki Uchida / Tomomi Kaneda / Mio Ono / Masao Matsuoka / Utano Nakamura / Akiko Ishida / Yoshimitsu Yamasaki / Hiroki Takeo / Takanobu Sakurai

Cosmetics, Vol 10, Iss 72, p

2023 Volume 72

Abstract: Glutathione (GSH) is present in almost all human cells and has a beneficial effect on human skin brightness. Cysteinylglycine (Cys-Gly) and γ-glutamylcysteine (γ-Glu-Cys) are GSH synthesis components. In this study, we defined glutathione (GSH), ... ...

Abstract	Glutathione (GSH) is present in almost all human cells and has a beneficial effect on human skin brightness. Cysteinylglycine (Cys-Gly) and γ-glutamylcysteine (γ-Glu-Cys) are GSH synthesis components. In this study, we defined glutathione (GSH), cysteinylglycine (Cys-Gly), and γ-glutamylcysteine (γ-Glu-Cys) as cysteine peptide and performed a randomized, double-blind, placebo-controlled study to investigate the effects of orally administered cysteine peptide on human skin brightness using a CM-26d portable spectrophotometer in healthy males and females aged between 20 and 65 years old. Eligible participants were randomly allocated into three groups (cysteine peptide 45 mg: n = 16, 90 mg: n = 15, and placebo: n = 16). Each subject ingested six tablets every day for 12 weeks, and skin brightness was measured at 0, 4, 8, and 12 weeks. As a result, the 45 mg group exhibited arm brightening in a time-dependent manner, and a significant difference was observed compared to the placebo at week 12 ( p = 0.028). Moreover, no serious adverse events and changes related to 270 mg study food were observed in the safety trial. Here, we suggest that cysteine peptide is a promising and safe compound for human skin brightness.
Keywords	cysteine peptide ; glutathione ; cysteinylglycine ; γ-glutamylcysteine ; skin brightness ; safety ; Chemistry ; QD1-999
Subject code	571
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis.

Suzuki, Minako / Okumura, Tomomi / Uchida, Koki / Ikeda, Yukinori / Tomooka, Yasuhiro / Nakajima, Tadaaki

FEBS open bio

2021 Volume 11, Issue 8, Page(s) 2282–2291

Abstract: Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long ... ...

Abstract	Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ-derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop-derived promoter and a lipofection reagent. GFP-positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines.
Language	English
Publishing date	2021-07-16
Publishing country	England
Document type	Journal Article
ZDB-ID	2651702-4
ISSN	2211-5463 ; 2211-5463
ISSN (online)	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13237
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Article ; Online: A Novel VIM-Type Metallo-β-Lactamase Variant, VIM-60, with Increased Hydrolyzing Activity against Fourth-Generation Cephalosporins in Pseudomonas aeruginosa Clinical Isolates in Japan.

Hishinuma, Tomomi / Tada, Tatsuya / Uchida, Hiroki / Shimojima, Masahiro / Kirikae, Teruo

Antimicrobial agents and chemotherapy

2019 Volume 63, Issue 6

Abstract: A novel VIM-type metallo-β-lactamase variant, VIM-60, was identified in multidrug- ... ...

Abstract	A novel VIM-type metallo-β-lactamase variant, VIM-60, was identified in multidrug-resistant
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Cefepime/pharmacology ; Cephalosporins/pharmacology ; Humans ; Hydrolysis ; Japan/epidemiology ; Microbial Sensitivity Tests ; Pseudomonas Infections/drug therapy ; Pseudomonas Infections/epidemiology ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/enzymology ; Pseudomonas aeruginosa/genetics ; beta-Lactamases/genetics ; Cefpirome
Chemical Substances	Anti-Bacterial Agents ; Cephalosporins ; Cefepime (807PW4VQE3) ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2019-05-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.00124-19
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Article ; Online: Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis

Minako Suzuki / Tomomi Okumura / Koki Uchida / Yukinori Ikeda / Yasuhiro Tomooka / Tadaaki Nakajima

FEBS Open Bio, Vol 11, Iss 8, Pp 2282-

2021 Volume 2291

Abstract	Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ‐derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop‐derived promoter and a lipofection reagent. GFP‐positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines.
Keywords	genetic transfection ; gonadal cells ; primary culture ; scallop ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2021-08-01T00:00:00Z
Publisher	Wiley
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dectin-2 Deficiency Promotes Proinflammatory Cytokine Release From Macrophages and Impairs Insulin Secretion.

Fujita, Masamichi / Miyazawa, Takashi / Uchida, Keiichiro / Uchida, Naohiro / Haji, Shojiro / Yano, Seiichi / Iwahashi, Norifusa / Hatayama, Tomomi / Katsuhara, Shunsuke / Nakamura, Shintaro / Takeichi, Yukina / Yokomoto-Umakoshi, Maki / Miyachi, Yasutaka / Sakamoto, Ryuichi / Iwakura, Yoichiro / Ogawa, Yoshihiro

Endocrinology

2023 Volume 165, Issue 1

Abstract: Pancreatic islet inflammation plays a crucial role in the etiology of type 2 diabetes (T2D). Macrophages residing in pancreatic islets have emerged as key players in islet inflammation. Macrophages express a plethora of innate immune receptors that bind ... ...

Abstract	Pancreatic islet inflammation plays a crucial role in the etiology of type 2 diabetes (T2D). Macrophages residing in pancreatic islets have emerged as key players in islet inflammation. Macrophages express a plethora of innate immune receptors that bind to environmental and metabolic cues and integrate these signals to trigger an inflammatory response that contributes to the development of islet inflammation. One such receptor, Dectin-2, has been identified within pancreatic islets; however, its role in glucose metabolism remains largely unknown. Here we have demonstrated that mice lacking Dectin-2 exhibit local inflammation within islets, along with impaired insulin secretion and β-cell dysfunction. Our findings indicate that these effects are mediated by proinflammatory cytokines, such as interleukin (IL)-1α and IL-6, which are secreted by macrophages that have acquired an inflammatory phenotype because of the loss of Dectin-2. This study provides novel insights into the mechanisms underlying the role of Dectin-2 in the development of islet inflammation.
MeSH term(s)	Animals ; Mice ; Cytokines/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Inflammation ; Insulin/metabolism ; Insulin Secretion ; Islets of Langerhans/metabolism ; Macrophages/metabolism
Chemical Substances	Cytokines ; dectin-2, mouse ; Insulin ; Clec4n protein, mouse
Language	English
Publishing date	2023-12-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	427856-2
ISSN	1945-7170 ; 0013-7227
ISSN (online)	1945-7170
ISSN	0013-7227
DOI	10.1210/endocr/bqad181
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Article ; Online: Association between personality traits and glycemic control after inpatient diabetes education.

Uchida, Taisuke / Ueno, Hiroaki / Konagata, Ayaka / Nakamura, Takayuki / Taniguchi, Norifumi / Nabekura, Hiroki / Kogo, Fumiko / Nagatomo, Yuma / Tanaka, Yuri / Shimizu, Koichiro / Shiiya, Tomomi / Yamaguchi, Hideki / Shimoda, Kazuya

Metabolism open

2023 Volume 18, Page(s) 100244

Abstract: Aims: The longitudinal effect of personality traits on glycemic control is unclear. This prospective observational study explored the relationship between personality traits and glycemic control in patients with uncontrolled diabetes after inpatient ... ...

Abstract	Aims: The longitudinal effect of personality traits on glycemic control is unclear. This prospective observational study explored the relationship between personality traits and glycemic control in patients with uncontrolled diabetes after inpatient diabetes education. Methods: Patients with diabetes mellitus (HbA1c ≥ 7.5%, measured by high-performance liquid chromatography) who received inpatient diabetes education were scored on the Big Five personality traits: neuroticism, extraversion, openness, agreeableness, and conscientiousness. Multiple linear analysis was used to determine whether any personality traits were independently associated with HbA1c on admission and HbA1c change from admission to 1, 3, and 6 months after discharge. Results: One hundred seventeen participants (mean age 60.4 ± 14.5 years; 59.0% male) were enrolled. HbA1c values on admission and 1, 3, and 6 months after discharge were 10.2 ± 2.1%, 8.3 ± 1.4%, 7.6 ± 1.4%, and 7.7 ± 1.5%, respectively. Multiple linear analysis showed that no personality traits were associated with HbA1c on admission. Neuroticism was negatively associated with the HbA1c change from admission to 3 months (β = -0.192, Conclusions: Neuroticism was associated with good long-term glycemic control after inpatient diabetes education.
Language	English
Publishing date	2023-04-14
Publishing country	England
Document type	Journal Article
ISSN	2589-9368
ISSN (online)	2589-9368
DOI	10.1016/j.metop.2023.100244
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Article ; Online: Emergence and spread of VIM-type metallo-β-lactamase-producing Pseudomonas aeruginosa clinical isolates in Japan.

Hishinuma, Tomomi / Uchida, Hiroki / Tohya, Mari / Shimojima, Masahiro / Tada, Tatsuya / Kirikae, Teruo

Journal of global antimicrobial resistance

2020 Volume 23, Page(s) 265–268

Abstract: Objectives: The aim of this study was to determine the genetic and epidemiological properties of Pseudomonas aeruginosa strains producing VIM-type metallo-β-lactamases isolated from patients in Japan.: Methods: A total of 1860 clinical isolates of ... ...

Abstract	Objectives: The aim of this study was to determine the genetic and epidemiological properties of Pseudomonas aeruginosa strains producing VIM-type metallo-β-lactamases isolated from patients in Japan. Methods: A total of 1860 clinical isolates of carbapenem-resistant P. aeruginosa were obtained from patients hospitalised in Japan from 2012-2018. Carbapenem-resistant P. aeruginosa isolates were screened for bla Results: Of 1860 isolates, 25 bla Conclusions: The number of P. aeruginosa strains producing VIM-type MBLs has increased in medical settings in Japan. Pseudomonas aeruginosa ST1816 producing VIM enzymes with Arg228Leu substitution have emerged and evolved in a medical setting in Japan.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Humans ; Japan/epidemiology ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; Phylogeny ; Pseudomonas Infections/drug therapy ; Pseudomonas Infections/epidemiology ; Pseudomonas aeruginosa/genetics ; beta-Lactamases/genetics
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2020-10-09
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2710046-7
ISSN	2213-7173 ; 2213-7165
ISSN (online)	2213-7173
ISSN	2213-7165
DOI	10.1016/j.jgar.2020.09.010
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Article ; Online: Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis

Suzuki, Minako / Okumura, Tomomi / Uchida, Koki / Ikeda, Yukinori / Tomooka, Yasuhiro / Nakajima, Tadaaki

FEBS Open Bio. 2021 Aug., v. 11, no. 8 p.2282-2291

2021

Abstract	Cell cultures can simplify assays of biological phenomena; therefore, cell culture systems have been established for many species, even invertebrates. However, there are few primary culture systems from marine invertebrates that can be maintained long term. The Japanese scallop, Patinopecten yessoensis, is a marine bivalve. Cell culture systems for the scallop have only been established for a few organ‐derived cell types and for embryonic cells. We developed a primary culture system for cells from male and female scallop gonads, hepatopancreas, and adductor muscle by utilizing culture conditions closer to those in nature, with regard to temperature, osmolarity, and nutrition. Primary cultured female gonadal cells were maintained for more than 1 month and had potential for proliferation. Furthermore, a genetic transfection system was attempted using a scallop‐derived promoter and a lipofection reagent. GFP‐positive cells were detected in the attempt. These technical developments would promote our understanding of biochemical mechanisms in scallops as well as providing clues for establishment of immortalized molluscan cell lines.
Keywords	Mizuhopecten yessoensis ; cell culture ; females ; gonads ; hepatopancreas ; males ; muscles ; nutrition ; osmolarity ; scallops ; temperature ; transfection
Language	English
Dates of publication	2021-08
Size	p. 2282-2291.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	2651702-4
ISSN	2211-5463
ISSN	2211-5463
DOI	10.1002/2211-5463.13237
Database	NAL-Catalogue (AGRICOLA)

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