LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 2 of total 2

Search options

  1. Article ; Online: Rapid molecular test for tuberculosis: impact of its routine use at a referral hospital.

    Casela, Marilda / Cerqueira, Silvânia Maria Andrade / Casela, Thais de Oliveira / Pereira, Mariana Araújo / Santos, Samanta Queiroz Dos / Pozo, Franco Andres Del / Freire, Songeli Menezes / Matos, Eliana Dias

    Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia

    2018  Volume 44, Issue 2, Page(s) 112–117

    Abstract: Objective: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia.: Methods: This was a ... ...

    Abstract Objective: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia.
    Methods: This was a descriptive study using the database of the Mycobacteriology Laboratory of the Octávio Mangabeira Specialized Hospital, in the city of Salvador, and georeferencing software. We evaluated 3,877 sputum samples collected from symptomatic respiratory patients, under routine conditions, between June of 2014 and March of 2015. All of the samples were submitted to sputum smear microscopy and the Xpert MTB/RIF test. Patients were stratified by gender, age, and geolocation.
    Results: Among the 3,877 sputum samples evaluated, the Xpert MTB/RIF test detected M. tuberculosis in 678 (17.5%), of which 60 (8.8%) showed resistance to rifampin. The Xpert MTB/RIF test detected M. tuberculosis in 254 patients who tested negative for sputum smear microscopy, thus increasing the diagnostic power by 59.9%.
    Conclusions: The use of the Xpert MTB/RIF test, under routine conditions, significantly increased the detection of cases of tuberculosis among sputum smear-negative patients.
    MeSH term(s) Adult ; Antibiotics, Antitubercular/therapeutic use ; Brazil ; Diagnostic Tests, Routine/methods ; Drug Resistance, Bacterial/drug effects ; Female ; Humans ; Male ; Microscopy/methods ; Middle Aged ; Molecular Diagnostic Techniques/methods ; Mycobacterium tuberculosis/drug effects ; Mycobacterium tuberculosis/isolation & purification ; Reference Values ; Reproducibility of Results ; Retrospective Studies ; Rifampin/therapeutic use ; Sensitivity and Specificity ; Sputum/microbiology ; Tertiary Care Centers ; Treatment Outcome ; Tuberculosis/diagnosis ; Tuberculosis/drug therapy ; Tuberculosis/microbiology
    Chemical Substances Antibiotics, Antitubercular ; Rifampin (VJT6J7R4TR)
    Language English
    Publishing date 2018-05-23
    Publishing country Brazil
    Document type Evaluation Studies ; Journal Article
    ZDB-ID 2223157-2
    ISSN 1806-3756 ; 1806-3713
    ISSN (online) 1806-3756
    ISSN 1806-3713
    DOI 10.1590/s1806-37562017000000201
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG+874 A>T polymorphism.

    Carneiro, Valdirene Leão / Bendicho, Maria Teresita / Santos, Rosalina Guedes / Casela, Marilda / Netto, Eduardo M / Mota, Scarlet Torres Moraes / Pina, Iza Cristina Araújo / Nascimento, Roberto Meyer / Freire, Songeli Menezes / Barbosa, Theolis

    The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

    2018  Volume 22, Issue 3, Page(s) 202–207

    Abstract: Introduction: Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low ... ...

    Abstract Introduction: Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG+874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG+874 A/T polymorphism on interferon-gamma test performance.
    Methods: Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated regarding the IFNG+874 genotype and the IFN-γ levels in whole blood cultures performed using an interferon-gamma commercial kit (QuantiFERON-TB
    Results: IFN-γ production was not influenced by the IFNG+874 genotype, regardless of antigen or mitogen-based stimulation, which suggests that other genes may influence IFN-γ production in response to mycobacteria. The IFNG+874 polymorphism was found to exert no influence over QFT-IT test sensitivity in our study.
    Conclusions: The IFNG+874 polymorphism was not shown to influence QuantiFERON-TB
    MeSH term(s) Brazil ; Case-Control Studies ; Female ; Gene Frequency ; Genotype ; Genotyping Techniques ; Humans ; Interferon-gamma/genetics ; Interferon-gamma/metabolism ; Interferon-gamma Release Tests/methods ; Male ; Mycobacterium tuberculosis/genetics ; Polymorphism, Genetic/genetics ; Reproducibility of Results ; Sensitivity and Specificity ; Statistics, Nonparametric ; Tuberculin Test ; Tuberculosis, Pulmonary/diagnosis
    Chemical Substances Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2018-05-19
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2041400-6
    ISSN 1678-4391 ; 1413-8670
    ISSN (online) 1678-4391
    ISSN 1413-8670
    DOI 10.1016/j.bjid.2018.04.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5633: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top