LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 110

Search options

  1. Article ; Online: A DNA rotary nanodevice operated by enzyme-initiated strand resetting.

    Chandrasekaran, Arun Richard

    Chemical communications (Cambridge, England)

    2024  Volume 60, Issue 5, Page(s) 534–537

    Abstract: DNA nanostructures that respond to external stimuli have found applications in several areas such as biosensing, drug delivery and molecular computation. The use of different types of stimuli in a single operation provides another layer of control for ... ...

    Abstract DNA nanostructures that respond to external stimuli have found applications in several areas such as biosensing, drug delivery and molecular computation. The use of different types of stimuli in a single operation provides another layer of control for the reconfiguration of nucleic acid nanostructures. This work demonstrates the use of a ribonuclease to "unset" a nucleic acid nanodevice based on the paranemic crossover (PX) DNA and specific DNA inputs to "reset" the structure into a juxtaposed DNA (JX
    MeSH term(s) Nucleic Acid Conformation ; DNA/chemistry ; Nucleic Acids ; Nanostructures/chemistry ; Ribonucleases ; Nanotechnology
    Chemical Substances DNA (9007-49-2) ; Nucleic Acids ; Ribonucleases (EC 3.1.-)
    Language English
    Publishing date 2024-01-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d3cc05487j
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: siRNA-loaded DNA nanostructures restore endothelial leakiness.

    Chandrasekaran, Arun Richard

    Nanoscale horizons

    2023  Volume 8, Issue 12, Page(s) 1606–1607

    Abstract: This article highlights the recent work of Qian, Leong, ... ...

    Abstract This article highlights the recent work of Qian, Leong, Wang
    MeSH term(s) RNA, Small Interfering/therapeutic use ; DNA/genetics ; Nanostructures
    Chemical Substances RNA, Small Interfering ; DNA (9007-49-2)
    Language English
    Publishing date 2023-11-20
    Publishing country England
    Document type Editorial
    ISSN 2055-6764
    ISSN (online) 2055-6764
    DOI 10.1039/d3nh90040a
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: DNA nanotechnology in the undergraduate laboratory: Electrophoretic analysis of DNA nanostructure biostability.

    Chandrasekaran, Arun Richard

    Journal of chemical education

    2022  Volume 100, Issue 1, Page(s) 316–320

    Abstract: The field of DNA nanotechnology has grown rapidly in the last decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still ... ...

    Abstract The field of DNA nanotechnology has grown rapidly in the last decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their involvement as interns in research laboratories. The DNA nanostructure biostability analysis experiment presented here can be used as a hands-on introductory laboratory exercise for discussing concepts in DNA nanotechnology in an undergraduate setting. This experiment discusses biostability, gel electrophoresis and quantitative analysis of nuclease degradation of a model DNA nanostructure, the paranemic crossover (PX) DNA motif. The experiment can be performed in a chemistry, biology or a biochemistry laboratory with minimal costs and can be adapted in undergraduate institutions using the instructor and student manuals provided here. Laboratory courses based on cutting edge research not only provide students a direct hands-on approach to the subject, but can also increase undergraduate student participation in research. Moreover, laboratory courses that reflect the increasingly multidisciplinary nature of research add value to undergraduate education.
    Language English
    Publishing date 2022-10-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 218164-2
    ISSN 1938-1328 ; 0021-9584
    ISSN (online) 1938-1328
    ISSN 0021-9584
    DOI 10.1021/acs.jchemed.2c00656
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 1902: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Undergraduate students in research: Accommodating undergraduates in the lab is a mutually beneficial relationship.

    Chandrasekaran, Arun Richard

    EMBO reports

    2021  Volume 22, Issue 6, Page(s) e53024

    Abstract: Giving undergraduate students an opportunity to partake in a research project pays back for both students and the lab. ...

    Abstract Giving undergraduate students an opportunity to partake in a research project pays back for both students and the lab.
    MeSH term(s) Humans ; Research Design ; Students
    Language English
    Publishing date 2021-05-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020896-0
    ISSN 1469-3178 ; 1469-221X
    ISSN (online) 1469-3178
    ISSN 1469-221X
    DOI 10.15252/embr.202153024
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5433: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 41: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: DNA-based ribonuclease detection assays.

    Chandrasekaran, Arun Richard

    Journal of materials chemistry. B

    2021  Volume 9, Issue 35, Page(s) 7023–7029

    Abstract: Ribonucleases are useful as biomarkers and can be the source of contamination in laboratory samples, making ribonuclease detection assays important in life sciences research. With recent developments in DNA-based biosensing, several new techniques are ... ...

    Abstract Ribonucleases are useful as biomarkers and can be the source of contamination in laboratory samples, making ribonuclease detection assays important in life sciences research. With recent developments in DNA-based biosensing, several new techniques are being developed to detect ribonucleases. This review discusses some of these methods, specifically those that utilize G-quadruplex DNA structures, DNA-nanoparticle conjugates and DNA nanostructures, and the advantages and challenges associated with them.
    MeSH term(s) Biocompatible Materials/chemistry ; Biosensing Techniques ; DNA/chemistry ; Materials Testing ; Nanostructures/chemistry ; Ribonucleases/analysis
    Chemical Substances Biocompatible Materials ; DNA (9007-49-2) ; Ribonucleases (EC 3.1.-)
    Language English
    Publishing date 2021-09-15
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/d1tb00300c
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Nuclease resistance of DNA nanostructures.

    Chandrasekaran, Arun Richard

    Nature reviews. Chemistry

    2021  Volume 5, Issue 4, Page(s) 225–239

    Abstract: DNA nanotechnology has progressed from proof-of-concept demonstrations of structural design towards application-oriented research. As a natural material with excellent self-assembling properties, DNA is an indomitable choice for various biological ... ...

    Abstract DNA nanotechnology has progressed from proof-of-concept demonstrations of structural design towards application-oriented research. As a natural material with excellent self-assembling properties, DNA is an indomitable choice for various biological applications, including biosensing, cell modulation, bioimaging and drug delivery. However, a major impediment to the use of DNA nanostructures in biological applications is their susceptibility to attack by nucleases present in the physiological environment. Although several DNA nanostructures show enhanced resistance to nuclease attack compared with duplexes and plasmid DNA, this may be inadequate for practical application. Recently, several strategies have been developed to increase the nuclease resistance of DNA nanostructures while retaining their functions, and the stability of various DNA nanostructures has been studied in biological fluids, such as serum, urine and cell lysates. This Review discusses the approaches used to modulate nuclease resistance in DNA nanostructures and provides an overview of the techniques employed to evaluate resistance to degradation and quantify stability.
    Language English
    Publishing date 2021-02-10
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2397-3358
    ISSN 2397-3358
    DOI 10.1038/s41570-021-00251-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: Nuclease resistance of DNA nanostructures.

    Chandrasekaran, Arun Richard

    Nature reviews. Chemistry

    2021  Volume 5, Issue 4, Page(s) 225–239

    Abstract: DNA nanotechnology has progressed from proof-of-concept demonstrations of structural design towards application-oriented research. As a natural material with excellent self-assembling properties, DNA is an indomitable choice for various biological ... ...

    Abstract DNA nanotechnology has progressed from proof-of-concept demonstrations of structural design towards application-oriented research. As a natural material with excellent self-assembling properties, DNA is an indomitable choice for various biological applications, including biosensing, cell modulation, bioimaging and drug delivery. However, a major impediment to the use of DNA nanostructures in biological applications is their susceptibility to attack by nucleases present in the physiological environment. Although several DNA nanostructures show enhanced resistance to nuclease attack compared with duplexes and plasmid DNA, this may be inadequate for practical application. Recently, several strategies have been developed to increase the nuclease resistance of DNA nanostructures while retaining their functions, and the stability of various DNA nanostructures has been studied in biological fluids, such as serum, urine and cell lysates. This Review discusses the approaches used to modulate nuclease resistance in DNA nanostructures and provides an overview of the techniques employed to evaluate resistance to degradation and quantify stability.
    Language English
    Publishing date 2021-02-10
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2397-3358
    ISSN (online) 2397-3358
    DOI 10.1038/s41570-021-00251-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Barium Concentration-Dependent Anomalous Electrophoresis of Synthetic DNA Motifs.

    Madhanagopal, Bharath Raj / Rodriguez, Arlin / Cordones, Mireylin / Chandrasekaran, Arun Richard

    ACS applied bio materials

    2024  

    Abstract: The structural integrity, assembly yield, and biostability of DNA nanostructures are influenced by the metal ions used to construct them. Although high (>10 mM) concentrations of divalent ions are often preferred for assembling DNA nanostructures, the ... ...

    Abstract The structural integrity, assembly yield, and biostability of DNA nanostructures are influenced by the metal ions used to construct them. Although high (>10 mM) concentrations of divalent ions are often preferred for assembling DNA nanostructures, the range of ion concentrations and the composition of the assembly products vary for different assembly conditions. Here, we examined the unique ability of Ba
    Language English
    Publishing date 2024-04-18
    Publishing country United States
    Document type Journal Article
    ISSN 2576-6422
    ISSN (online) 2576-6422
    DOI 10.1021/acsabm.4c00274
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Transitioning undergraduate research from wet lab to the virtual in the wake of a pandemic.

    Chandrasekaran, Arun Richard

    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology

    2020  Volume 48, Issue 5, Page(s) 436–438

    Abstract: The COVID-19 outbreak has shut down universities, and teaching faculty have moved to online classrooms to address students. This change has been supported by numerous online teaching tools and development of virtual classrooms. Undergraduate research ... ...

    Abstract The COVID-19 outbreak has shut down universities, and teaching faculty have moved to online classrooms to address students. This change has been supported by numerous online teaching tools and development of virtual classrooms. Undergraduate research programs in the sciences and biochemistry/molecular biology teaching labs, however, are affected by this change due to inaccessibility to laboratories. This communication outlines three concepts to engage undergraduate students who are involved in research: (a) remote data analysis, (b) literature review and science writing, and (c) science journal clubs.
    MeSH term(s) Biomedical Research/education ; COVID-19 ; Education, Distance ; Humans ; Molecular Biology/education ; Pandemics ; SARS-CoV-2 ; Students
    Keywords covid19
    Language English
    Publishing date 2020-06-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2039717-3
    ISSN 1539-3429 ; 1470-8175
    ISSN (online) 1539-3429
    ISSN 1470-8175
    DOI 10.1002/bmb.21386
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Processing DNA-Based Molecular Signals into Graphical Displays.

    Chandrasekaran, Arun Richard

    ACS synthetic biology

    2020  Volume 9, Issue 7, Page(s) 1490–1498

    Abstract: DNA is now well-established as a nanoscale building material with applications in fields such as biosensing and molecular computation. Molecular processes such as logic gates, nucleic acid circuits, and multiplexed detection have used different readout ... ...

    Abstract DNA is now well-established as a nanoscale building material with applications in fields such as biosensing and molecular computation. Molecular processes such as logic gates, nucleic acid circuits, and multiplexed detection have used different readout strategies to measure the output signal. In biosensing, this output can be the diagnosis of a disease biomarker, whereas in molecular computation, the output can be the result of a mathematical operation carried out using DNA. Recent developments have shown that the output of such processes can be displayed graphically as a macroscopic symbol or an alphanumeric character on multiwell plates, microarray chips, gels, lateral flow devices, and DNA origami surfaces. This review discusses the concepts behind such graphical readouts of molecular events, available display platforms, and the advantages and challenges in adapting such methods for practical use. Graphical display systems have the potential to be used in the creation of intelligent computing and sensing devices by which nanoscale binding events are translated into macroscopic visual readouts.
    MeSH term(s) Biosensing Techniques/instrumentation ; Biosensing Techniques/methods ; DNA/chemistry ; DNA/metabolism ; DNA Probes/chemistry ; DNA Probes/metabolism ; Logic ; Nanostructures/chemistry ; Nanotechnology ; Nucleic Acid Hybridization ; Signal Processing, Computer-Assisted
    Chemical Substances DNA Probes ; DNA (9007-49-2)
    Language English
    Publishing date 2020-06-25
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.0c00246
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top