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  1. Article ; Online: Lesion-Decoupling-Based Segmentation With Large-Scale Colon and Esophageal Datasets for Early Cancer Diagnosis.

    Lin, Qing / Tan, Weimin / Cai, Shilun / Yan, Bo / Li, Jichun / Zhong, Yunshi

    IEEE transactions on neural networks and learning systems

    2023  Volume PP

    Abstract: Lesions of early cancers often show flat, small, and isochromatic characteristics in medical endoscopy images, which are difficult to be captured. By analyzing the differences between the internal and external features of the lesion area, we propose a ... ...

    Abstract Lesions of early cancers often show flat, small, and isochromatic characteristics in medical endoscopy images, which are difficult to be captured. By analyzing the differences between the internal and external features of the lesion area, we propose a lesion-decoupling-based segmentation (LDS) network for assisting early cancer diagnosis. We introduce a plug-and-play module called self-sampling similar feature disentangling module (FDM) to obtain accurate lesion boundaries. Then, we propose a feature separation loss (FSL) function to separate pathological features from normal ones. Moreover, since physicians make diagnoses with multimodal data, we propose a multimodal cooperative segmentation network with two different modal images as input: white-light images (WLIs) and narrowband images (NBIs). Our FDM and FSL show a good performance for both single-modal and multimodal segmentations. Extensive experiments on five backbones prove that our FDM and FSL can be easily applied to different backbones for a significant lesion segmentation accuracy improvement, and the maximum increase of mean Intersection over Union (mIoU) is 4.58. For colonoscopy, we can achieve up to mIoU of 91.49 on our Dataset A and 84.41 on the three public datasets. For esophagoscopy, mIoU of 64.32 is best achieved on the WLI dataset and 66.31 on the NBI dataset.
    Language English
    Publishing date 2023-03-07
    Publishing country United States
    Document type Journal Article
    ISSN 2162-2388
    ISSN (online) 2162-2388
    DOI 10.1109/TNNLS.2023.3248804
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Wearable Microfluidic Sweat Chip for Detection of Sweat Glucose and pH in Long-Distance Running Exercise.

    Liu, Dong / Liu, Zhenyu / Feng, Shilun / Gao, Zehang / Chen, Ran / Cai, Gaozhe / Bian, Shengtai

    Biosensors

    2023  Volume 13, Issue 2

    Abstract: Traditional exercise training monitoring is based on invasive blood testing methods. As sweat can reveal abundant blood-related physiological information about health, wearable sweat sensors have received significant research attention and become ... ...

    Abstract Traditional exercise training monitoring is based on invasive blood testing methods. As sweat can reveal abundant blood-related physiological information about health, wearable sweat sensors have received significant research attention and become increasingly popular in the field of exercise training monitoring. However, most of these sensors are used to measure physical indicators such as heart rate, blood pressure, respiration, etc., demanding a versatile sensor that can detect relevant biochemical indicators in body fluids. In this work, we proposed a wearable microfluidic sweat chip combined with smartphone image processing to realize non-invasive in situ analysis of epidermal sweat for sports practitioners. The polydimethylsiloxane (PDMS) based chip was modified with nonionic surfactants to ensure good hydrophilicity for the automatic collection of sweat. Besides, a simple, reliable, and low-cost paper-based sensor was prepared for high-performance sensing of glucose concentration and pH in sweat. Under optimized conditions, this proposed chip can detect glucose with low concentrations from 0.05 mM to 0.40 mM, with a pH range of 4.0 to 6.5 for human sweat. The ability of this microfluidic chip for human sweat analysis was demonstrated by dynamically tracking the changes in glucose concentration and pH in long-distance running subjects.
    MeSH term(s) Humans ; Glucose/analysis ; Sweat/chemistry ; Wearable Electronic Devices ; Biosensing Techniques/methods ; Microfluidics ; Exercise ; Running ; Hydrogen-Ion Concentration
    Chemical Substances Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2023-01-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios13020157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Correction to: Improved submucosal tunneling endoscopic resection with slant tunnel for submucosal tumors in proximal esophagus.

    Di, Sun / Qiang, Shi / ZhiPeng, Qi / Bing, Li / Shilun, Cai / Pinghong, Zhou / Yunshi, Zhong

    Surgical endoscopy

    2021  Volume 36, Issue 3, Page(s) 2217

    Language English
    Publishing date 2021-08-31
    Publishing country Germany
    Document type Published Erratum
    ZDB-ID 639039-0
    ISSN 1432-2218 ; 0930-2794
    ISSN (online) 1432-2218
    ISSN 0930-2794
    DOI 10.1007/s00464-021-08624-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Digital metabolic activity assay enables fast assessment of 2D materials bactericidal efficiency.

    Wu, Wenshuai / Kiat Goh, Simon Chun / Cai, Gaozhe / Feng, Shilun / Zhang, Boran

    Analytica chimica acta

    2023  Volume 1285, Page(s) 342007

    Abstract: Background: The identification and quantification of viable Escherichia coli (E. coli) are important in multiple fields including the development of antimicrobial materials, water quality, food safety and infections diagnosis. However, the standard ... ...

    Abstract Background: The identification and quantification of viable Escherichia coli (E. coli) are important in multiple fields including the development of antimicrobial materials, water quality, food safety and infections diagnosis. However, the standard culture-based methods of viable E. coli detection suffer from long detection times (24 h) and complex operation, leaving the unmet requirement for fast assessing the efficiency of antimicrobial materials, early alerting the contamination of water and food, and immediately treatment of infections.
    Results: We present a digital β-d-glucuronidase (GUS) assay in a self-priming polydimethylsiloxane (PDMS) microfluidic chip for rapid E. coli identification and quantification. The GUS expression in viable bacteria was investigated to develop a fast GUS assay at the single-cell level. Single E. coli were stochastically discretized in picoliter chambers and identified by specific GUS activity. The digital GUS assay enabled identifying E. coli within 3 h and quantifying within 4 h for different E. coli subtypes. The specificity of our method was confirmed by using blended bacteria including E. coli, Bacillus, Shigella and Vibrio. We utilized digital GUS assay to enumerate viable E. coli after incubated with antibacterial materials for assessing the antibacterial efficiency. Moreover, the degassed chip can realize automatic sample distribution without external instruments.
    Significance: The results demonstrated the functionality and practicability of digital GUS assay for single E. coli identification and quantification. With air-tight packaging, the developed chip has the potential for on-site E. coli analysis and could be deployed for diagnosis of E. coli infections, antimicrobial susceptibility testing, and warning the fecal pollution of water. Digital GUS assay provides a paradigm, examining the activity of metabolic enzyme, for detecting the viable bacteria other than E. coli.
    MeSH term(s) Escherichia coli/metabolism ; Water Quality ; Microfluidics ; Anti-Bacterial Agents/pharmacology ; Glucuronidase/metabolism
    Chemical Substances Anti-Bacterial Agents ; Glucuronidase (EC 3.2.1.31)
    Language English
    Publishing date 2023-11-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2023.342007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: ClusterNet: a clustering distributed prior embedded detection network for early-stage esophageal squamous cell carcinoma diagnosis.

    Wang, Peisheng / Cai, Shilun / Tan, Weimin / Yan, Bo / Zhong, Yunshi

    Medical physics

    2022  Volume 50, Issue 2, Page(s) 854–866

    Abstract: Background: Early and accurate diagnosis of esophageal squamous cell carcinoma (ESCC) is important for reducing mortality. Analyzing intrapapillary capillary loops' (IPCLs) patterns on magnification endoscopy with narrow band imaging (ME-NBI) has been ... ...

    Abstract Background: Early and accurate diagnosis of esophageal squamous cell carcinoma (ESCC) is important for reducing mortality. Analyzing intrapapillary capillary loops' (IPCLs) patterns on magnification endoscopy with narrow band imaging (ME-NBI) has been demonstrated effective in the diagnosis of early-stage ESCC. However, even experienced endoscopists may face difficulty in finding and classifying countless IPCLs on ME-NBI.
    Purpose: We propose a novel clustering prior embedded detection network: ClusterNet. ClusterNet is capable of analyzing the distribution of IPCLs on ME-NBI automatically and enables endoscopists to overview multiple types of visualization. With ClusterNet assisting, endoscopists may observe ME-NBI images more efficiently, thus they may also predict the pathology and make medical decisions more easily.
    Methods: We propose the first large-scale ME-NBI dataset with fine-grained annotations by consensus of expert endoscopists. The dataset is splitted into a training set and an independent testing set based on patients. With two strategies for embedding, ClusterNet can automatically take the clustering effect into consideration. Prior to this work, none of the existing approaches take the clustering effect, which is rather important in classifying the IPCLs, into account.
    Results: ClusterNet achieves an average precision of 81.2% and an average recall of 90.0% for the detection of IPCLs patterns on each patient of the independent testing set. We also compare ClusterNet with other state-of-the-art detection approaches. The performance of ClusterNet with embedding strategies is consistently superior to that of other approaches in terms of average precision, recall and F2-Score.
    Conclusions: Experiments demonstrate that our proposed method is able to detect almost all the IPCLs patterns on ME-NBI and classify them according to the Japanese Endoscopic Society (JES) classification accurately.
    MeSH term(s) Humans ; Esophageal Squamous Cell Carcinoma/diagnostic imaging ; Esophageal Squamous Cell Carcinoma/pathology ; Esophageal Neoplasms/diagnostic imaging ; Carcinoma, Squamous Cell/pathology ; Esophagoscopy/methods ; Cluster Analysis
    Language English
    Publishing date 2022-10-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 188780-4
    ISSN 2473-4209 ; 0094-2405
    ISSN (online) 2473-4209
    ISSN 0094-2405
    DOI 10.1002/mp.16041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Label-free E. coli detection based on enzyme assay and a microfluidic slipchip.

    Cai, Gaozhe / Wu, Wenshuai / Feng, Shilun / Liu, Yuanjie

    The Analyst

    2021  Volume 146, Issue 14, Page(s) 4622–4629

    Abstract: An enzyme assay based method in a microfluidic slipchip was proposed for the rapid and label-free detection of E. coli. The specific target analyte of E. coli was β-d-glucuronidase (GUS) which could catalyze the substrate 6-chloro-4-methyl-umbelliferyl-β- ...

    Abstract An enzyme assay based method in a microfluidic slipchip was proposed for the rapid and label-free detection of E. coli. The specific target analyte of E. coli was β-d-glucuronidase (GUS) which could catalyze the substrate 6-chloro-4-methyl-umbelliferyl-β-d-glucuronide (6-CMUG) to release the fluorescent molecule 6-chloro-4-methyl-umbelliferyl (6-CMU). E. coli culture, lysis and enzymatic reaction steps could be conducted in a microfluidic slipchip without any pumps and valves, which was tailored for fluorescence detection using a commercial plate reader, to achieve a rapid E. coli test. A mixture of the culture broth, enzyme inducer and E. coli was injected into the chambers on the top layer. A mixture of the substrate and lysis solution was injected into the chambers on the bottom layer. Then, the slipchip was slid to make each chamber independent. E. coli was cultured in the chamber in the LB broth for 2.5 h. After that, the slipchip was slid again to introduce the lysis solution into the culture solution for GUS release and enzyme reaction, and then incubated in the plate reader at 42 °C for another 2.5 h. During incubation, the fluorescence intensity of each chamber was recorded. This proposed label-free method can directly detect E. coli with a low concentration of 8 CFU per chamber within 5 h, thus showing great potential in on-site E. coli detection.
    MeSH term(s) Biological Assay ; Enzyme Assays ; Escherichia coli ; Glucuronidase ; Microfluidics
    Chemical Substances Glucuronidase (EC 3.2.1.31)
    Language English
    Publishing date 2021-06-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d1an00495f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Magnetic Bead Manipulation in Microfluidic Chips for Biological Application.

    Cai, Gaozhe / Yang, Zixin / Chen, Yu-Cheng / Huang, Yaru / Liang, Lijuan / Feng, Shilun / Zhao, Jianlong

    Cyborg and bionic systems (Washington, D.C.)

    2023  Volume 4, Page(s) 23

    Abstract: Magnetic beads manipulation in microfluidic chips is a promising research field for biological application, especially in the detection of biological targets. In this review, we intend to present a thorough and in-depth overview of recent magnetic beads ... ...

    Abstract Magnetic beads manipulation in microfluidic chips is a promising research field for biological application, especially in the detection of biological targets. In this review, we intend to present a thorough and in-depth overview of recent magnetic beads manipulation in microfluidic chips and its biological application. First, we introduce the mechanism of magnetic manipulation in microfluidic chip, including force analysis, particle properties, and surface modification. Then, we compare some existing methods of magnetic manipulation in microfluidic chip and list their biological application. Besides, the suggestions and outlook for future developments in the magnetic manipulation system are also discussed and summarized.
    Language English
    Publishing date 2023-04-14
    Publishing country United States
    Document type Journal Article
    ISSN 2692-7632
    ISSN (online) 2692-7632
    DOI 10.34133/cbsystems.0023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Automatically optimized radiomics modeling system for small gastric submucosal tumor (<2 cm) discrimination based on EUS images.

    Cai, Mingyan / Song, Baohui / Deng, Yinhui / Gao, Pingting / Cai, Shilun / Yalikong, Ayimukedisi / Xu, Enpan / Zhong, Yunshi / Yu, Jinhua / Zhou, Pinghong

    Gastrointestinal endoscopy

    2023  Volume 99, Issue 4, Page(s) 537–547.e4

    Abstract: Background and aims: The clinical management of small gastric submucosal tumors (SMTs) (<2 cm) faces a non-negligible challenge because of the lack of guideline consensus and effective diagnostic tools. This article develops an automatically optimized ... ...

    Abstract Background and aims: The clinical management of small gastric submucosal tumors (SMTs) (<2 cm) faces a non-negligible challenge because of the lack of guideline consensus and effective diagnostic tools. This article develops an automatically optimized radiomics modeling system (AORMS) based on EUS images to diagnose and evaluate SMTs.
    Methods: A total of 205 patients with EUS images of small gastric SMTs (<2 cm) were retrospectively enrolled in the development phase of AORMS for the diagnosis and the risk stratification of GI stromal tumor (GIST). A total of 178 patients with images from different centers were prospectively enrolled in the independent testing phase. The performance of AORMS was compared to that of endoscopists in the development set and evaluated in the independent testing set.
    Results: AORMS demonstrated an area under the curve (AUC) of 0.762 for the diagnosis of GIST and 0.734 for the risk stratification of GIST, respectively. In the independent testing set, AORMS achieved an AUC of 0.770 and 0.750 for the diagnosis and risk stratification of small GISTs, respectively. In comparison, the AUCs of 5 experienced endoscopists ranged from 0.501 to 0.608 for diagnosing GIST and from 0.562 to 0.748 for risk stratification. AORMS outperformed experienced endoscopists by more than 20% in diagnosing GIST.
    Conclusions: AORMS implements automatic parameter selection, which enhances its robustness and clinical applicability. It has demonstrated good performance in the diagnosis and risk stratification of GISTs, which could aid endoscopists in the diagnosis of small gastric SMTs (<2 cm).
    MeSH term(s) Humans ; Gastrointestinal Stromal Tumors/pathology ; Radiomics ; Retrospective Studies ; Stomach Neoplasms/pathology ; Endosonography/methods
    Language English
    Publishing date 2023-11-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391583-9
    ISSN 1097-6779 ; 0016-5107
    ISSN (online) 1097-6779
    ISSN 0016-5107
    DOI 10.1016/j.gie.2023.11.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book ; Online: Colo-SCRL

    Chen, Qingzhong / Cai, Shilun / Cai, Crystal / Yu, Zefang / Qian, Dahong / Xiang, Suncheng

    Self-Supervised Contrastive Representation Learning for Colonoscopic Video Retrieval

    2023  

    Abstract: Colonoscopic video retrieval, which is a critical part of polyp treatment, has great clinical significance for the prevention and treatment of colorectal cancer. However, retrieval models trained on action recognition datasets usually produce ... ...

    Abstract Colonoscopic video retrieval, which is a critical part of polyp treatment, has great clinical significance for the prevention and treatment of colorectal cancer. However, retrieval models trained on action recognition datasets usually produce unsatisfactory retrieval results on colonoscopic datasets due to the large domain gap between them. To seek a solution to this problem, we construct a large-scale colonoscopic dataset named Colo-Pair for medical practice. Based on this dataset, a simple yet effective training method called Colo-SCRL is proposed for more robust representation learning. It aims to refine general knowledge from colonoscopies through masked autoencoder-based reconstruction and momentum contrast to improve retrieval performance. To the best of our knowledge, this is the first attempt to employ the contrastive learning paradigm for medical video retrieval. Empirical results show that our method significantly outperforms current state-of-the-art methods in the colonoscopic video retrieval task.

    Comment: Accepted by ICME 2023
    Keywords Computer Science - Computer Vision and Pattern Recognition
    Subject code 006 ; 004
    Publishing date 2023-03-27
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Construction and Manipulation of Serial Gradient Dilution Array on a Microfluidic Slipchip for Screening and Characterizing Inhibitors against Human Pancreatic Lipase.

    Yang, Junqiang / Deng, Yanyan / Zhang, Min / Feng, Shilun / Peng, Sheng / Yang, Shijia / Liu, Peirong / Cai, Gaozhe / Ge, Guangbo

    Biosensors

    2023  Volume 13, Issue 2

    Abstract: Obesity is one of the foremost public health concerns. Human pancreatic lipase (hPL), a crucial digestive enzyme responsible for the digestion of dietary lipids in humans, has been validated as an important therapeutic target for preventing and treating ... ...

    Abstract Obesity is one of the foremost public health concerns. Human pancreatic lipase (hPL), a crucial digestive enzyme responsible for the digestion of dietary lipids in humans, has been validated as an important therapeutic target for preventing and treating obesity. The serial dilution technique is commonly used to generate solutions with different concentrations and can be easily modified for drug screening. Conventional serial gradient dilution is often performed with tedious multiple manual pipetting steps, where it is difficult to precisely control fluidic volumes at low microliter levels. Herein, we presented a microfluidic SlipChip that enabled formation and manipulation of serial dilution array in an instrument-free manner. With simple slipping steps, the compound solution could be diluted to seven gradients with the dilution ratio of 1:1 and co-incubated with the enzyme (hPL)-substrate system for screening the anti-hPL potentials. To ensure complete mixing of solution and diluent during continuous dilution, we established a numerical simulation model and conducted an ink mixing experiment to determine the mixing time. Furthermore, we also demonstrated the serial dilution ability of the proposed SlipChip using standard fluorescent dye. As a proof of concept, we tested this microfluidic SlipChip using one marketed anti-obesity drug (Orlistat) and two natural products (1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) and sciadopitysin) with anti-hPL potentials. The IC
    MeSH term(s) Humans ; Microfluidics ; Orlistat ; Lipase ; Proteins ; Obesity ; Indicator Dilution Techniques
    Chemical Substances Orlistat (95M8R751W8) ; Lipase (EC 3.1.1.3) ; Proteins
    Language English
    Publishing date 2023-02-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios13020274
    Database MEDical Literature Analysis and Retrieval System OnLINE

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