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  1. Article ; Online: Coupling Differential Centrifugation with Exonuclease Treatment and Size Exclusion Chromatography (DIFSEC) for Purification of mtDNA from Mammalian Cells.

    Shaw, Andrew M / Gammage, Payam A

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2615, Page(s) 31–40

    Abstract: Direct analysis of mtDNA using PCR-free methods is limited by the presence of persistent, contaminating nucleic acids originating from the nuclear genome, even following stringent mitochondrial isolations. Here we describe a method developed in our ... ...

    Abstract Direct analysis of mtDNA using PCR-free methods is limited by the presence of persistent, contaminating nucleic acids originating from the nuclear genome, even following stringent mitochondrial isolations. Here we describe a method developed in our laboratory that couples existing, commercially available mtDNA isolation protocols with exonuclease treatment and size exclusion chromatography (DIFSEC). This protocol produces highly enriched mtDNA extracts from small-scale cell culture, with near-undetectable nuclear DNA contamination.
    MeSH term(s) Animals ; DNA, Mitochondrial/genetics ; Mitochondria/genetics ; Centrifugation ; Chromatography, Gel ; Polymerase Chain Reaction ; Mammals/genetics
    Chemical Substances DNA, Mitochondrial
    Language English
    Publishing date 2023-02-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2922-2_3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Spinal intramedullary uterine carcinosarcoma metastasis.

    Solis, Waldo / Youssef, Andrew M / Shaw, Richard / Li, Yingda

    BMJ case reports

    2024  Volume 17, Issue 2

    Abstract: Intramedullary spinal cord metastases (ISCM) are a rare and challenging manifestation of metastatic cancer that have devastating impacts on the individual's neurological function, survival expectancy and overall quality of life. Given the rarity and poor ...

    Abstract Intramedullary spinal cord metastases (ISCM) are a rare and challenging manifestation of metastatic cancer that have devastating impacts on the individual's neurological function, survival expectancy and overall quality of life. Given the rarity and poor prognosis, there is a lack of consensus in management. Uterine carcinosarcoma itself is a rare cancer, accounting for less than 3% of all uterine cancers. It carries a poor prognosis, with only one-third of patients surviving beyond 5 years. There are no previous reports of uterine carcinosarcoma metastases to the spinal cord. Here, we present the case of a woman in her late 70s with a uterine carcinosarcoma intramedullary metastasis that was refractory to radiotherapy treatment and responded favourably to surgical debulking.
    MeSH term(s) Female ; Humans ; Quality of Life ; Spinal Cord Neoplasms/diagnostic imaging ; Spinal Cord Neoplasms/surgery ; Spinal Cord Neoplasms/secondary ; Uterine Neoplasms/surgery ; Carcinosarcoma/surgery
    Language English
    Publishing date 2024-02-27
    Publishing country England
    Document type Case Reports ; Journal Article
    ISSN 1757-790X
    ISSN (online) 1757-790X
    DOI 10.1136/bcr-2023-259268
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  3. Article ; Online: A search for factors associated with reduced carbohydrate intake and NTD risk in two population-based studies.

    Shaw, Gary M / Yang, Wei / Weber, Kari A / Olshan, Andrew F / Desrosiers, Tania A

    Birth defects research

    2024  Volume 116, Issue 3, Page(s) e2328

    Abstract: Background: Two population-based case-control studies have reported an increased risk of neural tube defect (NTD)-affected pregnancies among women with low carbohydrate diet in the periconceptional period. Given that only two studies have investigated ... ...

    Abstract Background: Two population-based case-control studies have reported an increased risk of neural tube defect (NTD)-affected pregnancies among women with low carbohydrate diet in the periconceptional period. Given that only two studies have investigated this association, it is unclear to what degree the findings could be impacted by residual confounding. Here, we further interrogated both studies that observed this association with the objective to identify factors from a much larger number of factors that might explain the association.
    Methods: By employing a machine learning algorithm (random forest), we investigated a baseline set of over 200 variables. These analyses produced the top 10 variables in each data set for cases and controls that predicted periconceptional low carbohydrate intake.
    Results: Examining those prediction variables with logistic regression modeling, we did not observe any particular variable that substantially contributed to the NTD-low carbohydrate association in either data set.
    Conclusions: If there are underlying factors that explain the association, our findings suggest that none of the 200+ variables we examined were sufficiently correlated with what that true explanatory exposure may be. Alternatively, our findings may suggest that there are other unidentified factor(s) at play, or the association observed in two independent data sets is directly related to low carbohydrate intake.
    MeSH term(s) Pregnancy ; Humans ; Female ; Algorithms ; Diet, Carbohydrate-Restricted ; Machine Learning ; Neural Tube Defects
    Language English
    Publishing date 2024-03-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2104792-3
    ISSN 2472-1727
    ISSN (online) 2472-1727
    DOI 10.1002/bdr2.2328
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    Zs.A 749: Show issues Location:
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  4. Article ; Online: Progesterone activation of β

    North, Kelsey C / Shaw, Andrew A / Bukiya, Anna N / Dopico, Alex M

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 7248

    Abstract: Progesterone (≥1 µM) is used in recovery of cerebral ischemia, an effect likely contributed to by cerebrovascular dilation. The targets of this progesterone action are unknown. We report that micromolar (µM) progesterone activates mouse cerebrovascular ... ...

    Abstract Progesterone (≥1 µM) is used in recovery of cerebral ischemia, an effect likely contributed to by cerebrovascular dilation. The targets of this progesterone action are unknown. We report that micromolar (µM) progesterone activates mouse cerebrovascular myocyte BK channels; this action is lost in β
    MeSH term(s) Mice ; Animals ; Large-Conductance Calcium-Activated Potassium Channels/metabolism ; Progesterone/pharmacology ; Large-Conductance Calcium-Activated Potassium Channel beta Subunits/chemistry ; Large-Conductance Calcium-Activated Potassium Channel beta Subunits/genetics ; Large-Conductance Calcium-Activated Potassium Channel beta Subunits/metabolism ; Steroids/pharmacology ; Binding Sites ; Ethanol/pharmacology ; Oximes/pharmacology
    Chemical Substances Large-Conductance Calcium-Activated Potassium Channels ; Progesterone (4G7DS2Q64Y) ; Large-Conductance Calcium-Activated Potassium Channel beta Subunits ; Steroids ; Ethanol (3K9958V90M) ; Oximes
    Language English
    Publishing date 2023-11-09
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42827-w
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  5. Article ; Online: Sedative medications: an avoidable cause of asthma and COPD exacerbations?

    Chalitsios, Christos V / Fogarty, Andrew W / McKeever, Tricia M / Shaw, Dominick E

    The Lancet. Respiratory medicine

    2023  Volume 11, Issue 4, Page(s) e31–e32

    MeSH term(s) Humans ; Pulmonary Disease, Chronic Obstructive/drug therapy ; Asthma/chemically induced ; Asthma/drug therapy ; Disease Progression
    Language English
    Publishing date 2023-02-15
    Publishing country England
    Document type Letter
    ZDB-ID 2686754-0
    ISSN 2213-2619 ; 2213-2600
    ISSN (online) 2213-2619
    ISSN 2213-2600
    DOI 10.1016/S2213-2600(23)00042-5
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    Zs.A 7041: Show issues Location:
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  6. Article ; Online: What is the link between housing, crowding, infections and high rates of kidney disease in a remote Aboriginal town?

    Lansbury, Nina / Hoy, Wendy / Shaw, Barb / Barnes, Samuel K / Memmott, Paul / Redmond, Andrew M

    Australian and New Zealand journal of public health

    2023  Volume 47, Issue 2, Page(s) 100030

    MeSH term(s) Humans ; Housing ; Socioeconomic Factors ; Crowding ; Indigenous Peoples ; Kidney Diseases
    Language English
    Publishing date 2023-03-16
    Publishing country United States
    Document type Letter
    ZDB-ID 1323548-5
    ISSN 1753-6405 ; 1326-0200
    ISSN (online) 1753-6405
    ISSN 1326-0200
    DOI 10.1016/j.anzjph.2023.100030
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    Zs.A 1916: Show issues Location:
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  7. Article ; Online: Alcohol and pregnenolone interaction on cerebral arteries through targeting of vascular smooth muscle

    North, Kelsey C / Shaw, Andrew A / Moreira, Luiz / Bukiya, Anna N / Dopico, Alex M

    Advances in drug and alcohol research

    2023  Volume 3

    Abstract: Despite the significant number of people who may be taking pregnenolone supplements while drinking alcohol (ethanol), the widely documented cerebrovascular actions of pregnenolone and ethanol, and the critical dependence of cerebrovascular function on ... ...

    Abstract Despite the significant number of people who may be taking pregnenolone supplements while drinking alcohol (ethanol), the widely documented cerebrovascular actions of pregnenolone and ethanol, and the critical dependence of cerebrovascular function on cerebral artery diameter, there are no studies addressing the effect of pregnenolone + ethanol in combination on cerebral artery diameter. We investigated this by evaluating the effect of this combination on middle cerebral artery diameter in male and female C57BL/6J mice, both
    Language English
    Publishing date 2023-08-14
    Publishing country Switzerland
    Document type Journal Article
    ISSN 2674-0001
    ISSN (online) 2674-0001
    DOI 10.3389/adar.2023.11735
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  8. Article: The oral microbiome.

    Shaw, Liam P / Smith, Andrew M / Roberts, Adam P

    Emerging topics in life sciences

    2021  Volume 1, Issue 4, Page(s) 287–296

    Abstract: The human microbiome is receiving a great deal of attention as its role in health and disease becomes ever more apparent. The oral microbiome, perhaps due to the ease with which we can obtain samples, is arguably the most well-studied human microbiome to ...

    Abstract The human microbiome is receiving a great deal of attention as its role in health and disease becomes ever more apparent. The oral microbiome, perhaps due to the ease with which we can obtain samples, is arguably the most well-studied human microbiome to date. It is obvious, however, that we have only just begun to scratch the surface of the complex bacterial and bacterial-host interactions within this complex community. Here, we describe the factors which are known to influence the development of the seemingly globally conserved, core, oral microbiome and those which are likely to be responsible for the observed differences at the individual level. We discuss the paradoxical situation of maintaining a stable core microbiome which is at the same time incredibly resilient and adaptable to many different stresses encountered in the open environment of the oral cavity. Finally, we explore the interactions of the oral microbiome with the host and discuss the interactions underlying human health and disease.
    Language English
    Publishing date 2021-01-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2882721-1
    ISSN 2397-8554 ; 2397-8554 ; 2397-8562
    ISSN (online) 2397-8554
    ISSN 2397-8554 ; 2397-8562
    DOI 10.1042/ETLS20170040
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  9. Article ; Online: Corrigendum: Advances and potential of omics studies for understanding the development of food allergy.

    Sindher, Sayantani B / Chin, Andrew R / Aghaeepour, Nima / Prince, Lawrence / Maecker, Holden / Shaw, Gary M / Stevenson, David / Nadeau, Kari C / Snyder, Michael / Khatri, Purvesh / Boyd, Scott D / Winn, Virginia D / Angst, Martin S / Chinthrajah, R Sharon

    Frontiers in allergy

    2024  Volume 5, Page(s) 1373485

    Abstract: This corrects the article DOI: 10.3389/falgy.2023.1149008.]. ...

    Abstract [This corrects the article DOI: 10.3389/falgy.2023.1149008.].
    Language English
    Publishing date 2024-02-23
    Publishing country Switzerland
    Document type Published Erratum
    ISSN 2673-6101
    ISSN (online) 2673-6101
    DOI 10.3389/falgy.2024.1373485
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Fluid Therapy in the Critically Ill.

    Shaw, Andrew D / Bagshaw, Sean M

    Critical care clinics

    2018  Volume 34, Issue 2, Page(s) xiii–xiv

    MeSH term(s) Critical Care/methods ; Critical Illness/therapy ; Fluid Therapy/methods ; Humans
    Language English
    Publishing date 2018-01-31
    Publishing country United States
    Document type Editorial
    ZDB-ID 1006423-0
    ISSN 1557-8232 ; 0749-0704
    ISSN (online) 1557-8232
    ISSN 0749-0704
    DOI 10.1016/j.ccc.2018.01.002
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