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Article ; Online: Codelivery of TGFβ and Cox2 siRNA inhibits HCC by promoting T-cell penetration into the tumor and improves response to Immune Checkpoint Inhibitors.

Kim, Wookhyun / Ye, Zhou / Simonenko, Vera / Shahi, Aashirwad / Malikzay, Asra / Long, Steven Z / Xu, John J / Lu, Alan / Horng, Jau-Hau / Wu, Chang-Ru / Chen, Pei-Jer / Lu, Patrick Y / Evans, David M

NAR cancer

2024 Volume 6, Issue 1, Page(s) zcad059

Abstract: Upregulation of TGFβ and Cox2 in the tumor microenvironment results in blockade of T-cell penetration into the tumor. Without access to tumor antigens, the T-cell response will not benefit from administration of the immune checkpoint antibodies. We ... ...

Abstract	Upregulation of TGFβ and Cox2 in the tumor microenvironment results in blockade of T-cell penetration into the tumor. Without access to tumor antigens, the T-cell response will not benefit from administration of the immune checkpoint antibodies. We created an intravenous polypeptide nanoparticle that can deliver two siRNAs (silencing TGFβ and Cox2). Systemic administration in mice, bearing a syngeneic orthotopic hepatocellular carcinoma (HCC), delivers the siRNAs to various cells in the liver, and significantly reduces the tumor. At 2 mg/kg (BIW) the nanoparticle demonstrated a single agent action and induced tumor growth inhibition to undetectable levels after five doses. Reducing the siRNAs to 1mg/kg BIW demonstrated greater inhibition in the presence of PD-L1 mAbs. After only three doses BIW, we could still recover a smaller tumor and, in tumor sections, showed an increase in penetration of CD4+ and CD8+ T-cells deeper into the remaining tumor that was not evident in animals treated with non-silencing siRNA. The combination of TGFβ and Cox2 siRNA co-administered in a polypeptide nanoparticle can act as a novel therapeutic alone against HCC and may augment the activity of the immune checkpoint antibodies. Silencing TGFβ and Cox2 converts an immune excluded (cold) tumor into a T-cell inflamed (hot) tumor.
Language	English
Publishing date	2024-01-09
Publishing country	England
Document type	Journal Article
ISSN	2632-8674
ISSN (online)	2632-8674
DOI	10.1093/narcan/zcad059
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Recombinant protein polymers in biomaterials.

Kim, Wookhyun

Frontiers in bioscience (Landmark edition)

2013 Volume 18, Issue 1, Page(s) 289–304

Abstract: Naturally occurring protein-based materials have been found that function as critical components in biomechanical response, fibers and adhesives. A relatively small but growing number of recombinant protein-based materials that mimic the desired features ...

Abstract	Naturally occurring protein-based materials have been found that function as critical components in biomechanical response, fibers and adhesives. A relatively small but growing number of recombinant protein-based materials that mimic the desired features of their natural sources, such as collagens, elastins and silks, are considered as an alternative to conventional synthetic polymers. Advances in genetic engineering have facilitated the synthesis of repetitive protein polymers with precise control of molecular weights which are designed by using synthetic genes encoding tandem repeats of oligopeptide originating from a modular domain of natural proteins. Many repeat sequences as protein polymer building blocks adopt a well-defined secondary structure and undergo self-assembly to result in physically cross-linked networks or with chemical cross-linking so that further form three-dimensional architectures similar to natural counterparts. In this review, recombinant protein polymers currently developed will be presented that have emerged as promising class of next generation biomaterials.
MeSH term(s)	Animals ; Biocompatible Materials/chemical synthesis ; Biocompatible Materials/chemistry ; Biomimetic Materials/chemical synthesis ; Collagen/chemistry ; Elastin/chemistry ; Elastomers ; Insect Proteins/chemistry ; Protein Engineering/methods ; Protein Structure, Tertiary ; Proteins/chemistry ; Recombinant Proteins ; Repetitive Sequences, Amino Acid ; Silk/chemistry ; Spiders
Chemical Substances	Biocompatible Materials ; Elastomers ; Insect Proteins ; Proteins ; Recombinant Proteins ; Silk ; adhesive protein, mussel ; resilin (61790-52-1) ; Collagen (9007-34-5) ; Elastin (9007-58-3)
Language	English
Publishing date	2013-01-01
Publishing country	Singapore
Document type	Journal Article ; Review
ZDB-ID	2704569-9
ISSN	2768-6698 ; 1093-9946
ISSN (online)	2768-6698
ISSN	1093-9946
DOI	10.2741/4100
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Analysis of Visuo Motor Control between Dominant Hand and Non-Dominant Hand for Effective Human-Robot Collaboration.

Jo, Hanjin / Choi, Woong / Lee, Geonhui / Park, Wookhyun / Kim, Jaehyo

Sensors (Basel, Switzerland)

2020 Volume 20, Issue 21

Abstract: The human-in-the-loop technology requires studies on sensory-motor characteristics of each hand for an effective human-robot collaboration. This study aims to investigate the differences in visuomotor control between the dominant (DH) and non-dominant ... ...

Abstract	The human-in-the-loop technology requires studies on sensory-motor characteristics of each hand for an effective human-robot collaboration. This study aims to investigate the differences in visuomotor control between the dominant (DH) and non-dominant hands in tracking a target in the three-dimensional space. We compared the circular tracking performances of the hands on the frontal plane of the virtual reality space in terms of radial position error (Δ
MeSH term(s)	Adult ; Biomechanical Phenomena ; Feedback ; Female ; Hand ; Humans ; Male ; Movement ; Robotics ; Virtual Reality ; Young Adult
Language	English
Publishing date	2020-11-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s20216368
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Analysis of Control Characteristics between Dominant and Non-Dominant Hands by Transient Responses of Circular Tracking Movements in 3D Virtual Reality Space.

Park, Wookhyun / Choi, Woong / Jo, Hanjin / Lee, Geonhui / Kim, Jaehyo

Sensors (Basel, Switzerland)

2020 Volume 20, Issue 12

Abstract: Human movement is a controlled result of the sensory-motor system, and the motor control mechanism has been studied through diverse movements. The present study examined control characteristics of dominant and non-dominant hands by analyzing the ... ...

Abstract	Human movement is a controlled result of the sensory-motor system, and the motor control mechanism has been studied through diverse movements. The present study examined control characteristics of dominant and non-dominant hands by analyzing the transient responses of circular tracking movements in 3D virtual reality space. A visual target rotated in a circular trajectory at four different speeds, and 29 participants tracked the target with their hands. The position of each subject's hand was measured, and the following three parameters were investigated: normalized initial peak velocity (
MeSH term(s)	Hand ; Humans ; Movement ; Psychomotor Performance ; Virtual Reality
Language	English
Publishing date	2020-06-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s20123477
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Analysis of motor control strategy for frontal and sagittal planes of circular tracking movements using visual feedback noise from velocity change and depth information.

Geonhui Lee / Woong Choi / Hanjin Jo / Wookhyun Park / Jaehyo Kim

PLoS ONE, Vol 15, Iss 11, p e

2020 Volume 0241138

Abstract: We aim to investigate a control strategy for the circular tracking movement in a three-dimensional (3D) space based on the accuracy of the visual information. After setting the circular orbits for the frontal and sagittal planes in the 3D virtual space, ... ...

Abstract	We aim to investigate a control strategy for the circular tracking movement in a three-dimensional (3D) space based on the accuracy of the visual information. After setting the circular orbits for the frontal and sagittal planes in the 3D virtual space, the subjects track a target moving at a constant velocity. The analysis is applied to two parameters of the polar coordinates, namely, ΔR (the difference in the distance from the center of a circular orbit) and Δω (the difference in the angular velocity). The movement in the sagittal plane provides different depth information depending on the position of the target in orbit, unlike the task of the frontal plane. Therefore, the circular orbit is divided into four quadrants for a statistical analysis of ΔR. In the sagittal plane, the error was two to three times larger in quadrants 1 and 4 than in quadrants 2 and 3 close to the subject. Here, Δω is estimated using a frequency analysis; the lower the accuracy of the visual information, the greater the periodicity. When comparing two different planes, the periodicity in the sagittal plane was approximately 1.7 to 2 times larger than that of the frontal plane. In addition, the average angular velocity of the target and tracer was within 0.6% during a single cycle. We found that if the amount of visual information is reduced, an optimal feedback control strategy can be used to reduce the positional error within a specific area.
Keywords	Medicine ; R ; Science ; Q
Subject code	629
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Analysis of motor control strategy for frontal and sagittal planes of circular tracking movements using visual feedback noise from velocity change and depth information.

Lee, Geonhui / Choi, Woong / Jo, Hanjin / Park, Wookhyun / Kim, Jaehyo

PloS one

2020 Volume 15, Issue 11, Page(s) e0241138

Abstract	We aim to investigate a control strategy for the circular tracking movement in a three-dimensional (3D) space based on the accuracy of the visual information. After setting the circular orbits for the frontal and sagittal planes in the 3D virtual space, the subjects track a target moving at a constant velocity. The analysis is applied to two parameters of the polar coordinates, namely, ΔR (the difference in the distance from the center of a circular orbit) and Δω (the difference in the angular velocity). The movement in the sagittal plane provides different depth information depending on the position of the target in orbit, unlike the task of the frontal plane. Therefore, the circular orbit is divided into four quadrants for a statistical analysis of ΔR. In the sagittal plane, the error was two to three times larger in quadrants 1 and 4 than in quadrants 2 and 3 close to the subject. Here, Δω is estimated using a frequency analysis; the lower the accuracy of the visual information, the greater the periodicity. When comparing two different planes, the periodicity in the sagittal plane was approximately 1.7 to 2 times larger than that of the frontal plane. In addition, the average angular velocity of the target and tracer was within 0.6% during a single cycle. We found that if the amount of visual information is reduced, an optimal feedback control strategy can be used to reduce the positional error within a specific area.
MeSH term(s)	Adult ; Feedback, Sensory/physiology ; Female ; Humans ; Male ; Motion Perception/physiology ; Virtual Reality ; Young Adult
Language	English
Publishing date	2020-11-11
Publishing country	United States
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0241138
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Recombinant elastin-mimetic biomaterials: Emerging applications in medicine.

Kim, Wookhyun / Chaikof, Elliot L

Advanced drug delivery reviews

2010 Volume 62, Issue 15, Page(s) 1468–1478

Abstract: Biomaterials derived from protein-based block copolymers are increasingly investigated for potential application in medicine. In particular, recombinant elastin block copolymers provide significant opportunities to modulate material microstructure and ... ...

Abstract	Biomaterials derived from protein-based block copolymers are increasingly investigated for potential application in medicine. In particular, recombinant elastin block copolymers provide significant opportunities to modulate material microstructure and can be processed in various forms, including particles, films, gels, and fiber networks. As a consequence, biological and mechanical responses of elastin-based biomaterials are tunable through precise control of block size and amino acid sequence. In this review, the synthesis of a set of elastin-mimetic triblock copolymers and their diverse processing methods for generating material platforms currently applied in medicine will be discussed.
MeSH term(s)	Animals ; Biocompatible Materials/chemistry ; Biomimetic Materials/chemistry ; Elastin/chemistry ; Humans ; Protein Engineering/methods ; Recombinant Proteins/chemistry
Chemical Substances	Biocompatible Materials ; Recombinant Proteins ; Elastin (9007-58-3)
Language	English
Publishing date	2010-05-02
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	639113-8
ISSN	1872-8294 ; 0169-409X
ISSN (online)	1872-8294
ISSN	0169-409X
DOI	10.1016/j.addr.2010.04.007
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 2241: Show issues

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Article ; Online: Blocking nocturnal blue light for insomnia: A randomized controlled trial.

Shechter, Ari / Kim, Elijah Wookhyun / St-Onge, Marie-Pierre / Westwood, Andrew J

Journal of psychiatric research

2017 Volume 96, Page(s) 196–202

Abstract: The use of light-emitting electronic devices before bedtime may contribute to or exacerbate sleep problems. Exposure to blue-wavelength light in particular from these devices may affect sleep by suppressing melatonin and causing neurophysiologic arousal. ...

Abstract	The use of light-emitting electronic devices before bedtime may contribute to or exacerbate sleep problems. Exposure to blue-wavelength light in particular from these devices may affect sleep by suppressing melatonin and causing neurophysiologic arousal. We aimed to determine if wearing amber-tinted blue light-blocking lenses before bedtime improves sleep in individuals with insomnia. Fourteen individuals (n = 8 females; age ± SD 46.6 ± 11.5 y) with insomnia symptoms wore blue light-blocking amber lenses or clear placebo lenses in lightweight wraparound frames for 2 h immediately preceding bedtime for 7 consecutive nights in a randomized crossover trial (4-wk washout). Ambulatory sleep measures included the Pittsburgh Insomnia Rating Scale (PIRS) completed at the end of each intervention period, and daily post-sleep questionnaire and wrist-actigraphy. PIRS total scores, and Quality of Life, Distress, and Sleep Parameter subscales, were improved in amber vs. clear lenses condition (p-values <0.05). Reported wake-time was significantly delayed, and mean subjective total sleep time (TST), overall quality, and soundness of sleep were significantly higher (p-values <0.05) in amber vs. clear lenses condition over the 7-d intervention period. Actigraphic measures of TST only were significantly higher in amber vs. clear lenses condition (p = 0.035). Wearing amber vs. clear lenses for 2-h preceding bedtime for 1 week improved sleep in individuals with insomnia symptoms. These findings have health relevance given the broad use of light-emitting devices before bedtime and prevalence of insomnia. Amber lenses represent a safe, affordable, and easily implemented therapeutic intervention for insomnia symptoms. Clinical trials registration: ClinicalTrials.gov Identifier: NCT02698800.
MeSH term(s)	Actigraphy ; Adolescent ; Adult ; Aged ; Cross-Over Studies ; Female ; Humans ; Lenses ; Male ; Middle Aged ; Phototherapy/instrumentation ; Severity of Illness Index ; Sleep/radiation effects ; Sleep Initiation and Maintenance Disorders/therapy ; Surveys and Questionnaires ; Time Factors ; Treatment Outcome ; Young Adult
Language	English
Publishing date	2017-10-21
Publishing country	England
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	3148-3
ISSN	1879-1379 ; 0022-3956
ISSN (online)	1879-1379
ISSN	0022-3956
DOI	10.1016/j.jpsychires.2017.10.015
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Ui I Zs.243: Show issues

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Article ; Online: Designing protein-based biomaterials for medical applications.

Gagner, Jennifer E / Kim, Wookhyun / Chaikof, Elliot L

Acta biomaterialia

2013 Volume 10, Issue 4, Page(s) 1542–1557

Abstract: Biomaterials produced by nature have been honed through billions of years, evolving exquisitely precise structure-function relationships that scientists strive to emulate. Advances in genetic engineering have facilitated extensive investigations to ... ...

Abstract	Biomaterials produced by nature have been honed through billions of years, evolving exquisitely precise structure-function relationships that scientists strive to emulate. Advances in genetic engineering have facilitated extensive investigations to determine how changes in even a single peptide within a protein sequence can produce biomaterials with unique thermal, mechanical and biological properties. Elastin, a naturally occurring protein polymer, serves as a model protein to determine the relationship between specific structural elements and desirable material characteristics. The modular, repetitive nature of the protein facilitates the formation of well-defined secondary structures with the ability to self-assemble into complex three-dimensional architectures on a variety of length scales. Furthermore, many opportunities exist to incorporate other protein-based motifs and inorganic materials into recombinant protein-based materials, extending the range and usefulness of these materials in potential biomedical applications. Elastin-like polypeptides (ELPs) can be assembled into 3-D architectures with precise control over payload encapsulation, mechanical and thermal properties, as well as unique functionalization opportunities through both genetic and enzymatic means. An overview of current protein-based materials, their properties and uses in biomedicine will be provided, with a focus on the advantages of ELPs. Applications of these biomaterials as imaging and therapeutic delivery agents will be discussed. Finally, broader implications and future directions of these materials as diagnostic and therapeutic systems will be explored.
MeSH term(s)	Animals ; Biocompatible Materials/chemical synthesis ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Biomedical Technology/methods ; Elastin/chemistry ; Elastin/pharmacology ; Humans ; Peptides/chemistry ; Peptides/pharmacology ; Proteins/chemistry ; Recombinant Proteins/pharmacology
Chemical Substances	Biocompatible Materials ; Peptides ; Proteins ; Recombinant Proteins ; Elastin (9007-58-3)
Language	English
Publishing date	2013-10-09
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2173841-5
ISSN	1878-7568 ; 1742-7061
ISSN (online)	1878-7568
ISSN	1742-7061
DOI	10.1016/j.actbio.2013.10.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Amphiphilic protein micelles for targeted in vivo imaging

Kim, Wookhyun / Brady, Colin / Chaikof, Elliot L

Acta Biomaterialia. 2012 July, v. 8, no. 7

2012

Abstract: A variety of polymeric nanoparticles have been developed for bioimaging applications. This study reports on the use of a 50nm recombinant protein nanoparticle with a multivalent surface as a vehicle for functionalization with a model imaging agent. ... ...

Abstract	A variety of polymeric nanoparticles have been developed for bioimaging applications. This study reports on the use of a 50nm recombinant protein nanoparticle with a multivalent surface as a vehicle for functionalization with a model imaging agent. Multiple fluorescent probes were covalently conjugated to surface amines of crosslinked amphiphilic elastin-mimetic protein micelles using N-hydroxysuccinimide ester chemistry. In vivo fluorescence imaging confirmed that protein micelles selectively accumulated at sites of angioplasty induced vessel wall injury, presumably via an enhanced permeability and retention effect. This investigation demonstrates the potential of amphiphilic protein micelles to be used as a vehicle for selective imaging of sites associated with a disrupted or leaky endothelium.
Keywords	amines ; crosslinking ; endothelium ; fluorescence ; image analysis ; micelles ; nanoparticles ; permeability
Language	English
Dates of publication	2012-07
Size	p. 2476-2482.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2173841-5
ISSN	1878-7568 ; 1742-7061
ISSN (online)	1878-7568
ISSN	1742-7061
DOI	10.1016/j.actbio.2012.04.011
Database	NAL-Catalogue (AGRICOLA)

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