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Article: Oblique injection depth correction by a two parallel OCT sensor guided handheld SMART injector.

Im, Jintaek / Song, Cheol

2021 Volume 12, Issue 2, Page(s) 926–939

Abstract: We present a SMART injector with two parallel common-path optical coherence tomography fibers to enable angle measurements and injection depth corrections for oblique subretinal injection. The two optical fibers are attached to opposite sides of a 33 G ... ...

Abstract	We present a SMART injector with two parallel common-path optical coherence tomography fibers to enable angle measurements and injection depth corrections for oblique subretinal injection. The two optical fibers are attached to opposite sides of a 33 G needle with known offsets and designed to pass through a 23 G trocar that has an inner diameter of 0.65 mm. By attaching a SMART system to a rotational stage, the measured angles are calibrated for minimal error from reference angles. A commercial eye model was used to evaluate the control performance, and injection experiments were performed on a phantom made of agarose gel and a porcine eye.
Language	English
Publishing date	2021-01-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2572216-5
ISSN	2156-7085
ISSN	2156-7085
DOI	10.1364/BOE.410492
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Lipoteichoic acid of

Kim, Sun Kyung / Im, Jintaek / Ko, Eun Byeol / Lee, Dongwook / Seo, Ho Seong / Yun, Cheol-Heui / Han, Seung Hyun

Frontiers in immunology

2023 Volume 14, Page(s) 1056949

Abstract: Streptococcus ... ...

Abstract	Streptococcus gordonii
MeSH term(s)	Humans ; Streptococcus gordonii/metabolism ; Cytokines/metabolism ; Lipopolysaccharides/pharmacology ; Lipopolysaccharides/metabolism ; Dendritic Cells
Chemical Substances	lipoteichoic acid (56411-57-5) ; Cytokines ; Lipopolysaccharides
Language	English
Publishing date	2023-03-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1056949
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Lipoproteins are key immunostimulatory components of Bacillus species for dendritic cell maturation and activation.

Jeong, Sungho / Im, Jintaek / Lee, Dongwook / Ko, Kwang Hyun / Yun, Cheol-Heui / Han, Seung Hyun

Molecular immunology

2023 Volume 165, Page(s) 82–91

Abstract: Dendritic cells (DCs) play an important role in immunity by sensing and responding to invasive microbes. Bacillus species are rod-shaped sporulating bacteria that include the pathogenic Bacillus cereus and commensal Bacillus subtilis. Although the ... ...

Abstract	Dendritic cells (DCs) play an important role in immunity by sensing and responding to invasive microbes. Bacillus species are rod-shaped sporulating bacteria that include the pathogenic Bacillus cereus and commensal Bacillus subtilis. Although the interaction between DC and these two Bacillus species has been studied, their key structural component that prompts DC activation is poorly understood. Here, we investigated the two Bacillus species in DC activation by whole cells and their representative microbe-associated molecular patterns (MAMPs). MAMPs including lipoteichoic acid (LTA), lipoprotein (LPP), and peptidoglycan (PGN) were purified from the two Bacillus species. Among the MAMPs, LPP from both species most potently induced the maturation and activation of DCs while PGN, but not LTA, moderately stimulated DCs. LPPs from both Bacillus species enhanced the expression of DC maturation markers including CCR7, CD40, CD80, CD83, CD86, CD205, MHC-I, and MHC-II. Among the MAMPs from B. cereus, PGN most considerably lowered the endocytic capacity of DCs implying DC maturation whereas PGN from B. subtilis lowered it to a similar degree to its LPP. Furthermore, DCs sensitized with LPPs from both Bacillus species and PGN from B. subtilis moderately induced TNF-α and IL-6 production. Notably, a combination of MAMPs did not show any synergistic effect on DC activation. Taken together, our results demonstrate that LPP is the key structural component in B. cereus and B. subtilis that leads to DC activation.
MeSH term(s)	Bacillus/metabolism ; Cell Differentiation ; Tumor Necrosis Factor-alpha/metabolism ; Transcription Factors/metabolism ; Dendritic Cells ; Lipoproteins/metabolism ; Cytokines/metabolism
Chemical Substances	Tumor Necrosis Factor-alpha ; Transcription Factors ; Lipoproteins ; Cytokines
Language	English
Publishing date	2023-12-30
Publishing country	England
Document type	Journal Article
ZDB-ID	424427-8
ISSN	1872-9142 ; 0161-5890
ISSN (online)	1872-9142
ISSN	0161-5890
DOI	10.1016/j.molimm.2023.12.009
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Article ; Online: Fibroblasts from patients with idiopathic pulmonary fibrosis are resistant to cisplatin-induced cell death via enhanced CK2-dependent XRCC1 activity.

Im, Jintaek / Nho, Richard Seonghun

Apoptosis : an international journal on programmed cell death

2019 Volume 24, Issue 5-6, Page(s) 499–510

Abstract: Idiopathic pulmonary fibrosis (IPF) is a deadly and progressive fibrotic lung disease, but the precise etiology remains elusive. IPF is characterized by the presence of apoptosis-resistant (myo)fibroblasts that relentlessly produce a collagen-rich ... ...

Abstract	Idiopathic pulmonary fibrosis (IPF) is a deadly and progressive fibrotic lung disease, but the precise etiology remains elusive. IPF is characterized by the presence of apoptosis-resistant (myo)fibroblasts that relentlessly produce a collagen-rich extracellular matrix (ECM). Recent studies showed that an anti-cancer chemotherapy drug cisplatin is implicated in the development of pulmonary fibrosis, suggesting that the treatment of cancer patients with cisplatin may alter fibroblast viability. To address this possibility, we investigated the cisplatin-induced cell death mechanism in lung fibroblasts derived from IPF and non-IPF patients in response to a collagen matrix. IPF fibroblasts showed enhanced resistance to cisplatin-induced cell death compared to non-IPF fibroblasts in a time- and dose-dependent manner. Molecular study showed that the expression of γH2AX, PUMA and caspase-3/7 activity was abnormally reduced in IPF fibroblasts, suggesting that DNA damage-induced apoptosis caused by cisplatin was suppressed in IPF fibroblasts. Our study further revealed that DNA repair protein XRCC1 activity was aberrantly increased as a result of CK2 hyper-activation in cisplatin-treated IPF fibroblasts, and this alteration protected IPF fibroblasts from cisplatin-induced cell death. Our results showed that IPF fibroblasts residing in a collagen rich matrix are resistance to cisplatin-induced cell death due to the aberrantly high CK2/XRCC1-dependent DNA repair activity. This finding suggests that pulmonary fibrosis may develop and worsen due to the presence of apoptosis-resistant lung fibroblasts in cisplatin-treated cancer patients.
MeSH term(s)	Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Casein Kinase II/antagonists & inhibitors ; Casein Kinase II/genetics ; Casein Kinase II/metabolism ; Cell Death/drug effects ; Cell Survival/drug effects ; Cells, Cultured ; Cisplatin/pharmacology ; DNA Damage ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Gene Expression ; Humans ; Idiopathic Pulmonary Fibrosis/pathology ; RNA, Small Interfering ; X-ray Repair Cross Complementing Protein 1/genetics ; X-ray Repair Cross Complementing Protein 1/metabolism
Chemical Substances	Antineoplastic Agents ; RNA, Small Interfering ; X-ray Repair Cross Complementing Protein 1 ; XRCC1 protein, human ; Casein Kinase II (EC 2.7.11.1) ; Cisplatin (Q20Q21Q62J)
Language	English
Publishing date	2019-03-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1452360-7
ISSN	1573-675X ; 1360-8185
ISSN (online)	1573-675X
ISSN	1360-8185
DOI	10.1007/s10495-019-01529-9
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Zs.A 5178: Show issues

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Article: The Novel

Lee, Dongwook / Im, Jintaek / Na, Hongjun / Ryu, Sangryeol / Yun, Cheol-Heui / Han, Seung Hyun

Frontiers in microbiology

2019 Volume 10, Page(s) 2877

Abstract: Enterococcus ... ...

Abstract	Enterococcus faecalis
Language	English
Publishing date	2019-12-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2019.02877
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Article ; Online: Enhanced location tracking in sensor fusion-assisted virtual reality micro-manipulation environments.

John David Prieto Prada / Jintaek Im / Hyondong Oh / Cheol Song

PLoS ONE, Vol 16, Iss 12, p e

2021 Volume 0261933

Abstract: Virtual reality (VR) technology plays a significant role in many biomedical applications. These VR scenarios increase the valuable experience of tasks requiring great accuracy with human subjects. Unfortunately, commercial VR controllers have large ... ...

Abstract	Virtual reality (VR) technology plays a significant role in many biomedical applications. These VR scenarios increase the valuable experience of tasks requiring great accuracy with human subjects. Unfortunately, commercial VR controllers have large positioning errors in a micro-manipulation task. Here, we propose a VR-based framework along with a sensor fusion algorithm to improve the microposition tracking performance of a microsurgical tool. To the best of our knowledge, this is the first application of Kalman filter in a millimeter scale VR environment, by using the position data between the VR controller and an inertial measuring device. This study builds and tests two cases: (1) without sensor fusion tracking and (2) location tracking with active sensor fusion. The static and dynamic experiments demonstrate that the Kalman filter can provide greater precision during micro-manipulation in small scale VR scenarios.
Keywords	Medicine ; R ; Science ; Q
Subject code	629
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Enhanced location tracking in sensor fusion-assisted virtual reality micro-manipulation environments.

Prieto Prada, John David / Im, Jintaek / Oh, Hyondong / Song, Cheol

PloS one

2021 Volume 16, Issue 12, Page(s) e0261933

Abstract	Virtual reality (VR) technology plays a significant role in many biomedical applications. These VR scenarios increase the valuable experience of tasks requiring great accuracy with human subjects. Unfortunately, commercial VR controllers have large positioning errors in a micro-manipulation task. Here, we propose a VR-based framework along with a sensor fusion algorithm to improve the microposition tracking performance of a microsurgical tool. To the best of our knowledge, this is the first application of Kalman filter in a millimeter scale VR environment, by using the position data between the VR controller and an inertial measuring device. This study builds and tests two cases: (1) without sensor fusion tracking and (2) location tracking with active sensor fusion. The static and dynamic experiments demonstrate that the Kalman filter can provide greater precision during micro-manipulation in small scale VR scenarios.
MeSH term(s)	Adult ; Algorithms ; Computers ; Equipment Design ; Female ; Humans ; Male ; Man-Machine Systems ; Micromanipulation ; Movement ; Reproducibility of Results ; Software ; User-Computer Interface ; Virtual Reality
Language	English
Publishing date	2021-12-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0261933
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Serotype-Dependent Inhibition of

Lim, Suwon / Lee, Dongwook / Jeong, Sungho / Park, Jeong Woo / Im, Jintaek / Choi, Bokeum / Gwak, Donghyun / Yun, Cheol-Heui / Seo, Ho Seong / Han, Seung Hyun

Journal of microbiology and biotechnology

2023 Volume 34, Issue 1, Page(s) 47–55

Abstract: Streptococcus ... ...

Abstract	Streptococcus pneumoniae
MeSH term(s)	Humans ; Infant ; Streptococcus pneumoniae ; Serogroup ; Propionates/pharmacology ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/prevention & control ; Anti-Bacterial Agents/pharmacology ; Pneumococcal Vaccines/pharmacology ; Fatty Acids, Volatile ; Butyrates/pharmacology ; Vaccines, Conjugate ; Acetates/pharmacology ; Serotyping
Chemical Substances	Propionates ; Anti-Bacterial Agents ; Pneumococcal Vaccines ; Fatty Acids, Volatile ; Butyrates ; Vaccines, Conjugate ; Acetates
Language	English
Publishing date	2023-11-20
Publishing country	Korea (South)
Document type	Journal Article
ZDB-ID	2412195-2
ISSN	1738-8872 ; 1738-8872
ISSN (online)	1738-8872
ISSN	1738-8872
DOI	10.4014/jmb.2309.09003
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Article ; Online: Bacillus subtilis Induces Human Beta Defensin-2 Through its Lipoproteins in Human Intestinal Epithelial Cells.

So, Yoon Ju / Park, Ok-Jin / Kwon, Yeongkag / Im, Jintaek / Lee, Dongwook / Yun, Sung-Ho / Cho, Kun / Yun, Cheol-Heui / Han, Seung Hyun

Probiotics and antimicrobial proteins

2024

Abstract: Human intestinal epithelial cells (IECs) play an important role in maintaining gut homeostasis by producing antimicrobial peptides (AMPs). Bacillus subtilis, a commensal bacterium, is considered a probiotic. Although its protective effects on intestinal ... ...

Abstract	Human intestinal epithelial cells (IECs) play an important role in maintaining gut homeostasis by producing antimicrobial peptides (AMPs). Bacillus subtilis, a commensal bacterium, is considered a probiotic. Although its protective effects on intestinal health are widely reported, the key component of B. subtilis responsible for its beneficial effects remains elusive. In this study, we tried to identify the key molecules responsible for B. subtilis-induced AMPs and their molecular mechanisms in a human IEC line, Caco-2. B. subtilis increased human beta defensin (HBD)-2 mRNA expression in a dose- and time-dependent manner. Among the B. subtilis microbe-associated molecular patterns, lipoprotein (LPP) substantially increased the mRNA expression and protein production of HBD-2, whereas lipoteichoic acid and peptidoglycan did not show such effects. Those results were confirmed in primary human IECs. In addition, both LPP recognition and HBD-2 secretion mainly took place on the apical side of fully differentiated and polarized Caco-2 cells through Toll-like receptor 2-mediated JNK/p38 MAP kinase/AP-1 and NF-κB pathways. HBD-2 efficiently inhibited the growth of the intestinal pathogens Staphylococcus aureus and Bacillus cereus. Furthermore, LPPs pre-incubated with lipase or proteinase K decreased LPP-induced HBD-2 expression, suggesting that the lipid and protein moieties of LPP are crucial for HBD-2 expression. Q Exactive Plus mass spectrometry identified 35 B. subtilis LPP candidates within the LPP preparation, and most of them were ABC transporters. Taken together, these results suggest that B. subtilis promotes HBD-2 secretion in human IECs mainly with its LPPs, which might enhance the protection from intestinal pathogens.
Language	English
Publishing date	2024-02-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2487792-X
ISSN	1867-1314 ; 1867-1306
ISSN (online)	1867-1314
ISSN	1867-1306
DOI	10.1007/s12602-024-10224-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: TLR3 recognition of viral double-stranded RNA in human dental pulp cells is important for the innate immunity.

Ha, Ye-Eun / Ju So, Yoon / Im, Jintaek / Yun, Cheol-Heui / Park, Joo-Cheol / Hyun Han, Seung

International immunopharmacology

2023 Volume 119, Page(s) 110161

Abstract: Dental caries or trauma can expose human dental pulp cells (DPCs) to various oral microorganisms, which play an important role in the development of an innate immune response. In the present study, we examined the expression of Toll-like receptors (TLRs) ...

Abstract	Dental caries or trauma can expose human dental pulp cells (DPCs) to various oral microorganisms, which play an important role in the development of an innate immune response. In the present study, we examined the expression of Toll-like receptors (TLRs) for sensing microbe-associated molecular patterns in human DPCs. Interestingly, real-time PCR analysis demonstrated that TLR3 is the most highly expressed among 10 different TLRs in human DPCs. Poly(I:C), a representative TLR3 ligand mimicking viral double-stranded RNA, potently induced IL-8 expression in a time- and dose-dependent manner. Concordantly, poly(I:C) treatment substantially increased the expression of pro-inflammatory cytokines and chemokines such as IL-6, CCL2, and CXCL10. Human DPCs transfected with TLR3 siRNA exhibited decreased IL-8 production compared with non-targeting siRNA-transfected cells, suggesting that the expression of poly(I:C)-induced inflammatory cytokines is dependent on TLR3. IL-8 secretion induced by poly(I:C) was down-regulated by MAP kinase inhibitors, indicating that the MAP kinase pathway contributes to IL-8 production. Furthermore, C/EBPβ and NF-κB were essential transcriptional factors for poly(I:C)-induced IL-8 expression, as demonstrated by the transient transfection and reporter gene assay. Since lipoproteins are known as major immunostimulatory components of bacteria, human DPCs were treated with poly(I:C) together with Pam2CSK4, a synthetic lipopeptide mimicking bacterial lipoproteins. Pam2CSK4 and poly(I:C) co-treatment synergistically increased IL-8 production in comparison to Pam2CSK4 or poly(I:C) alone, implying that co-infection of viruses and bacteria can synergistically induce inflammatory responses in the dental pulp. Taken together, these results suggest that human DPCs potentially sense and respond to viral double-stranded RNAs, leading to effective induction of innate immune responses.
MeSH term(s)	Humans ; Toll-Like Receptor 3/metabolism ; RNA, Double-Stranded ; Interleukin-8/genetics ; Interleukin-8/pharmacology ; Dental Caries ; Dental Pulp/metabolism ; Cytokines/metabolism ; Immunity, Innate ; Poly I-C/pharmacology ; Toll-Like Receptors/genetics ; RNA, Small Interfering/pharmacology ; Cells, Cultured
Chemical Substances	Toll-Like Receptor 3 ; RNA, Double-Stranded ; Interleukin-8 ; Cytokines ; Poly I-C (O84C90HH2L) ; Toll-Like Receptors ; RNA, Small Interfering ; TLR3 protein, human
Language	English
Publishing date	2023-04-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2023.110161
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