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Article ; Online: First report of oestrosis in aoudad from southeastern Spain.

Barroso, Patricia / Ruiz-de-Ybáñez, Rocío / Martínez-Carrasco, Carlos / Gens, María J / Escribano, Fernando / Sánchez, Antonio / Pérez, Jesús M

Parasitology research

2017 Volume 116, Issue 7, Page(s) 2053–2055

Abstract: In spring 2016, we analyzed the skull of 44 aoudads (Ammotragus lervia) from Sierra Espuña Regional Park (SE Spain) for the presence of oestrid larvae. Oestrus ovis larvae were found in 27.3% of sampled hosts, with a mean intensity of 1.5 ± 6.1 larvae/ ... ...

Abstract	In spring 2016, we analyzed the skull of 44 aoudads (Ammotragus lervia) from Sierra Espuña Regional Park (SE Spain) for the presence of oestrid larvae. Oestrus ovis larvae were found in 27.3% of sampled hosts, with a mean intensity of 1.5 ± 6.1 larvae/per parasitized host (range 1-21). To our knowledge, this is the first report of oestrosis affecting this host species.
MeSH term(s)	Animals ; Diptera ; Female ; Larva ; Male ; Myiasis/parasitology ; Myiasis/veterinary ; Ruminants/parasitology ; Seasons ; Spain
Language	English
Publishing date	2017-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	284966-5
ISSN	1432-1955 ; 0932-0113 ; 0044-3255
ISSN (online)	1432-1955
ISSN	0932-0113 ; 0044-3255
DOI	10.1007/s00436-017-5504-0
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Article ; Online: Paradoxical effects of JZL184, an inhibitor of monoacylglycerol lipase, on bone remodelling in healthy and cancer-bearing mice.

Marino, Silvia / de Ridder, Daniëlle / Bishop, Ryan T / Renema, Nathalie / Ponzetti, Marco / Sophocleous, Antonia / Capulli, Mattia / Aljeffery, Abdullah / Carrasco, Giovana / Gens, Marianela Dalghi / Khogeer, Asim / Ralston, Stuart H / Gertsch, Jürg / Lamoureux, Francois / Heymann, Dominique / Rucci, Nadia / Idris, Aymen I

EBioMedicine

2019 Volume 44, Page(s) 452–466

Abstract: Background: Cancer-associated bone disease is a serious complication in bone sarcomas and metastatic carcinomas of breast and prostate origin. Monoacylglycerol lipase (MAGL) is an enzyme of the endocannabinoid system, and is responsible for the ... ...

Abstract	Background: Cancer-associated bone disease is a serious complication in bone sarcomas and metastatic carcinomas of breast and prostate origin. Monoacylglycerol lipase (MAGL) is an enzyme of the endocannabinoid system, and is responsible for the degradation of the most abundant endocannabinoid in bone, 2-arachidonoyl glycerol (2AG). Methods: The effects of the verified MAGL inhibitor on bone remodelling were assessed in healthy mice and in mouse models of bone disease caused by prostate and breast cancers and osteosarcoma. Findings: JZL184 reduced osteolytic bone metastasis in mouse models of breast and prostate cancers, and inhibited skeletal tumour growth, metastasis and the formation of ectopic bone in models of osteosarcoma. Additionally, JZL184 suppressed cachexia and prolonged survival in mice injected with metastatic osteosarcoma and osteotropic cancer cells. Functional and histological analysis revealed that the osteoprotective action of JZL184 in cancer models is predominately due to inhibition of tumour growth and metastasis. In the absence of cancer, however, exposure to JZL184 exerts a paradoxical reduction of bone volume via an effect that is mediated by both Cnr1 and Cnr2 cannabinoid receptors. Interpretation: MAGL inhibitors such as JZL184, or its novel analogues, may be of value in the treatment of bone disease caused by primary bone cancer and bone metastasis, however, activation of the skeletal endocannabinoid system may limit their usefulness as osteoprotective agents.
MeSH term(s)	Animals ; Benzodioxoles/pharmacology ; Bone Neoplasms/diagnosis ; Bone Neoplasms/drug therapy ; Bone Neoplasms/metabolism ; Bone Neoplasms/secondary ; Bone Remodeling/drug effects ; Bone Resorption/diagnostic imaging ; Bone Resorption/drug therapy ; Bone Resorption/metabolism ; Bone Resorption/pathology ; Bone and Bones/diagnostic imaging ; Bone and Bones/drug effects ; Bone and Bones/metabolism ; Bone and Bones/pathology ; Cell Communication/drug effects ; Disease Models, Animal ; Enzyme Inhibitors/pharmacology ; Female ; Heterografts ; Humans ; Mice ; Monoacylglycerol Lipases/antagonists & inhibitors ; Osteoclasts/drug effects ; Osteoclasts/metabolism ; Osteolysis/drug therapy ; Osteolysis/etiology ; Osteolysis/metabolism ; Osteolysis/pathology ; Piperidines/pharmacology ; Receptors, Cannabinoid/metabolism
Chemical Substances	Benzodioxoles ; Enzyme Inhibitors ; JZL 184 ; Piperidines ; Receptors, Cannabinoid ; Monoacylglycerol Lipases (EC 3.1.1.23)
Language	English
Publishing date	2019-05-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2851331-9
ISSN	2352-3964
ISSN (online)	2352-3964
DOI	10.1016/j.ebiom.2019.05.048
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Article ; Online: Molecular cues involved in the regulation of B cell dynamics: Assistants of antigen hunting.

Carrasco, Yolanda R

Journal of leukocyte biology

2020 Volume 107, Issue 6, Page(s) 1107–1113

Abstract: The ability of a cell to migrate, adhere, and change its morphology is determinant in developing its functions; these capacities reach their maximum relevance in immune cells. For an efficient immune response, immune cells must localize in the right ... ...

Abstract	The ability of a cell to migrate, adhere, and change its morphology is determinant in developing its functions; these capacities reach their maximum relevance in immune cells. For an efficient immune response, immune cells must localize in the right place at the right time; that implies crossing tissue barriers and migrating in the interstitial space of the tissues at high velocities. The dependency on trafficking abilities is even higher for B cells, one of the arms of the adaptive immune system, considering that they must encounter specific antigens for their clonal receptor in the enormous tissue volume of the secondary lymphoid organs (spleen, lymph nodes, Peyer patches). The regulated interplay between cell motility and cell adhesion allows B cells to reach distinct lymphoid tissues and, within them, to explore the stromal cell networks where antigen might be exposed. In this meeting-invited review, I summarize the current knowledge on the molecular cues and mechanisms that shapes B cell dynamics at the initial phase of the humoral immune response, including homeostatic chemoattractants and innate/inflammatory stimuli. I also revised the B cell behavior alterations caused by BCR recognition of antigen and the molecular mechanisms involved.
MeSH term(s)	Animals ; B-Lymphocytes/cytology ; B-Lymphocytes/immunology ; Cell Adhesion ; Cell Movement ; Gene Expression ; Homeostasis/immunology ; Humans ; Immunity, Humoral ; Immunity, Innate ; Immunological Synapses ; Lymph Nodes/cytology ; Lymph Nodes/immunology ; Peyer's Patches/cytology ; Peyer's Patches/immunology ; Receptors, Antigen, B-Cell/genetics ; Receptors, Antigen, B-Cell/immunology ; Spleen/cytology ; Spleen/immunology ; Stromal Cells/cytology ; Stromal Cells/immunology ; Toll-Like Receptors/genetics ; Toll-Like Receptors/immunology
Chemical Substances	Receptors, Antigen, B-Cell ; Toll-Like Receptors
Language	English
Publishing date	2020-04-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	605722-6
ISSN	1938-3673 ; 0741-5400
ISSN (online)	1938-3673
ISSN	0741-5400
DOI	10.1002/JLB.1MR0220-276R
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Article ; Online: Paradoxical effects of JZL184, an inhibitor of monoacylglycerol lipase, on bone remodelling in healthy and cancer-bearing miceResearch in context

Silvia Marino / Daniëlle de Ridder / Ryan T. Bishop / Nathalie Renema / Marco Ponzetti / Antonia Sophocleous / Mattia Capulli / Abdullah Aljeffery / Giovana Carrasco / Marianela Dalghi Gens / Asim Khogeer / Stuart H. Ralston / Jürg Gertsch / Francois Lamoureux / Dominique Heymann / Nadia Rucci / Aymen I. Idris

EBioMedicine, Vol 44, Iss , Pp 452-

2019 Volume 466

Abstract	Background: Cancer-associated bone disease is a serious complication in bone sarcomas and metastatic carcinomas of breast and prostate origin. Monoacylglycerol lipase (MAGL) is an enzyme of the endocannabinoid system, and is responsible for the degradation of the most abundant endocannabinoid in bone, 2-arachidonoyl glycerol (2AG). Methods: The effects of the verified MAGL inhibitor on bone remodelling were assessed in healthy mice and in mouse models of bone disease caused by prostate and breast cancers and osteosarcoma. Findings: JZL184 reduced osteolytic bone metastasis in mouse models of breast and prostate cancers, and inhibited skeletal tumour growth, metastasis and the formation of ectopic bone in models of osteosarcoma. Additionally, JZL184 suppressed cachexia and prolonged survival in mice injected with metastatic osteosarcoma and osteotropic cancer cells. Functional and histological analysis revealed that the osteoprotective action of JZL184 in cancer models is predominately due to inhibition of tumour growth and metastasis. In the absence of cancer, however, exposure to JZL184 exerts a paradoxical reduction of bone volume via an effect that is mediated by both Cnr1 and Cnr2 cannabinoid receptors. Interpretation: MAGL inhibitors such as JZL184, or its novel analogues, may be of value in the treatment of bone disease caused by primary bone cancer and bone metastasis, however, activation of the skeletal endocannabinoid system may limit their usefulness as osteoprotective agents. Keywords: MAGL, Cannabinoid, Breast, Prostate, Bone, Cancer, Sarcoma, Osteolysis, Osteoclast, Metastasis
Keywords	Medicine ; R ; Medicine (General) ; R5-920
Subject code	616
Language	English
Publishing date	2019-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: First report of oestrosis in aoudad from southeastern Spain

Barroso, Patricia / RocÃo Ruiz-de-YbÃ¡Ã±ez / Carlos MartÃnez-Carrasco / MarÃa J. Gens / Fernando Escribano / Antonio SÃ¡nchez / JesÃºs M. PÃ©rez

Parasitology research. 2017 July, v. 116, no. 7

2017

Abstract: In spring 2016, we analyzed the skull of 44 aoudads (Ammotragus lervia) from Sierra EspuÃ±a Regional Park (SE Spain) for the presence of oestrid larvae. Oestrus ovis larvae were found in 27.3% of sampled hosts, with a mean intensity of 1.5Â Â±Â 6.1 ... ...

Abstract	In spring 2016, we analyzed the skull of 44 aoudads (Ammotragus lervia) from Sierra EspuÃ±a Regional Park (SE Spain) for the presence of oestrid larvae. Oestrus ovis larvae were found in 27.3% of sampled hosts, with a mean intensity of 1.5Â Â±Â 6.1 larvae/per parasitized host (range 1â21). To our knowledge, this is the first report of oestrosis affecting this host species.
Keywords	Ammotragus lervia ; Oestrus ovis ; hosts ; larvae ; parasitism ; parasitoses ; skull ; spring ; Spain
Language	English
Dates of publication	2017-07
Size	p. 2053-2055.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	284966-5
ISSN	1432-1955 ; 0932-0113 ; 0044-3255
ISSN (online)	1432-1955
ISSN	0932-0113 ; 0044-3255
DOI	10.1007/s00436-017-5504-0
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: CRISPR/Cas9-mediated genome editing assists protein dynamics studies in live cells.

Carrasco-Padilla, Carlos / Roda-Navarro, Pedro

European journal of cell biology

2022 Volume 101, Issue 2, Page(s) 151203

Abstract: Spatial and temporal regulation of molecular reactions dictates cell fate. Thus, studying molecular dynamics is essential to understand how cells decide what to do and the fundamental perturbations causing disease. Classically, molecular dynamics has ... ...

Abstract	Spatial and temporal regulation of molecular reactions dictates cell fate. Thus, studying molecular dynamics is essential to understand how cells decide what to do and the fundamental perturbations causing disease. Classically, molecular dynamics has been studied by protocols based in the overexpression of fluorescent fusion proteins. However, overexpression is associated to altered stoichiometry, molecular dynamics and subcellular distribution. We here discuss the necessity to study molecular dynamics of fluorescent fusion proteins expressed under physiological mechanisms in the cell, pointing to CRISPR/Cas9-mediated genome editing as the ideal means to do so. Current genome editing protocols enable us to study molecular dynamics while avoiding drawbacks associated to overexpression.
MeSH term(s)	CRISPR-Cas Systems/genetics ; Gene Editing/methods
Language	English
Publishing date	2022-01-29
Publishing country	Germany
Document type	Journal Article
ZDB-ID	391967-5
ISSN	1618-1298 ; 0070-2463 ; 0171-9335
ISSN (online)	1618-1298
ISSN	0070-2463 ; 0171-9335
DOI	10.1016/j.ejcb.2022.151203
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Article ; Online: Reduced Free Fatty Acid Receptor 4 Gene Expression is Associated With Extreme Obesity and Insulin Resistance in Children.

Codoñer-Alejos, Alan / Carrasco-Luna, Joaquín / Carrasco-García, Álvaro / Codoñer-Franch, Pilar

Journal of pediatric gastroenterology and nutrition

2021 Volume 74, Issue 4, Page(s) 535–540

Abstract: Objectives: Free fatty acid receptor 4 (FFAR4) is a G-protein-coupled membrane receptor highly expressed in macrophages that triggers anti-inflammatory effects and promotes insulin sensitization. We have previously found significant associations between ...

Abstract	Objectives: Free fatty acid receptor 4 (FFAR4) is a G-protein-coupled membrane receptor highly expressed in macrophages that triggers anti-inflammatory effects and promotes insulin sensitization. We have previously found significant associations between the FFAR4 rs11187533 single nucleotide polymorphism (SNP) and various obesity comorbidity parameters. We aimed to verify the FFAR4 expression levels in children with obesity and the associated comorbidities. Methods: Thirty-eight children with obesity were studied. Clinical and anthropometric evaluation was performed. A venous sample under fasting conditions was obtained. Biochemical study included parameters of metabolic risk. DNA was extracted and genotyped for the rs11187533 FFAR4 SNP. Real-time PCR technique was performed to investigate the gene expression. Relative FFAR4 mRNA levels were determined according to the 2-ΔΔCt method. Results: Significant differences in FFAR4 expression levels between the CC and CT-TT genotypes of the rs11187533 FFAR4 SNP were observed (P = 0.034). The minor allele T presented higher levels of FFAR4 expression. We found that a loss of FFAR4 expression was associated with extreme obesity (P = 0.032). The lowest FFAR4 expression levels were observed in children who had higher insulin (P = 0.008) and homeostasis model assessment insulin resistance values (P = 0.012) and lower quantitative insulin-sensitivity check index (P = 0.033). Conclusions: The underexpression of FFAR4 was associated with extreme obesity and parameters indicative of obesity comorbidities in children. This under expression could be partially influenced by the presence of the C allele rs11187533 FFAR4 SNP.
MeSH term(s)	Child ; Fatty Acids, Nonesterified ; Gene Expression ; Humans ; Insulin ; Insulin Resistance/genetics ; Obesity/genetics ; Polymorphism, Single Nucleotide ; Receptors, G-Protein-Coupled/genetics
Chemical Substances	FFAR4 protein, human ; Fatty Acids, Nonesterified ; Insulin ; Receptors, G-Protein-Coupled
Language	English
Publishing date	2021-11-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	603201-1
ISSN	1536-4801 ; 0277-2116
ISSN (online)	1536-4801
ISSN	0277-2116
DOI	10.1097/MPG.0000000000003360
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Book ; Conference proceedings: Regulation of gene expression in animal viruses

Carrasco, Luis

[proceedings of a NATO Advanced Study Institute on Regulation of Gene Expression in Animal Viruses, held May 30 - June 8, 1992, in Mallorca, Spain]

(NATO ASI series : Series A, life sciences ; 240)

1993

Event/congress	Advanced Study Institute on Regulation of Gene Expression in Animal Viruses (1992, PalmadeMallorca)
Author's details	ed. by Luis Carrasco
Series title	NATO ASI series : Series A, life sciences ; 240 NATO ASI series NATO ASI series ; Series A, life sciences
Collection	NATO ASI series NATO ASI series ; Series A, life sciences
Keywords	Gene Expression Regulation, Viral / congresses ; Genes, Viral / congresses ; Viren ; Genregulation ; Genexpression ; Virusinfketion ; Cytogenetik
Subject	Zellgenetik ; Zytogenetik ; Virus ; Genetische Regulation ; Transkription ; Genaktivität
Language	English
Size	VIII, 325 S. : Ill., graph. Darst.
Publisher	Plenum Press
Publishing place	New York u.a.
Publishing country	United States
Document type	Book ; Conference proceedings
HBZ-ID	HT004968404
ISBN	0-306-44408-9 ; 978-0-306-44408-1
Database	Catalogue ZB MED Medicine, Health

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Article ; Online: Epilepsia dependiente de piridoxina por deficiencia en el gen PNPO.

Garcia-Ezquiaga, J / Carrasco-Marina, M L / Gutierrez-Cruz, N / Iglesias-Escalera, G / Castro-Reguera, M / Perez-Gonzalez, B

Revista de neurologia

2019 Volume 69, Issue 7, Page(s) 303–304

Title translation	Pyridoxine-dependent epilepsy due to deficiency in the PNPO gene.
MeSH term(s)	Adolescent ; Brain Diseases, Metabolic/genetics ; Chromosomes, Human, Pair 17/genetics ; Epilepsy/cerebrospinal fluid ; Epilepsy/drug therapy ; Epilepsy/genetics ; Female ; Humans ; Hypoxia-Ischemia, Brain/genetics ; Mutation, Missense ; Polymorphism, Single Nucleotide ; Pyridoxaminephosphate Oxidase/deficiency ; Pyridoxaminephosphate Oxidase/genetics ; Pyridoxine/therapeutic use ; Seizures/genetics ; Tetrahydrofolates/cerebrospinal fluid ; Uniparental Disomy
Chemical Substances	Tetrahydrofolates ; Pyridoxaminephosphate Oxidase (EC 1.4.3.5) ; Pyridoxine (KV2JZ1BI6Z) ; 5-methyltetrahydrofolate (TYK22LML8F)
Language	Spanish
Publishing date	2019-09-17
Publishing country	Spain
Document type	Case Reports ; Letter
ZDB-ID	1468278-3
ISSN	1576-6578 ; 0210-0010
ISSN (online)	1576-6578
ISSN	0210-0010
DOI	10.33588/rn.6907.2019084
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Article ; Online: Recommendations for the elaboration of diagnostic genetic reports in the clinical setting.

Oriola, Josep / Carrasco, Pilar / Díez, Orland / Ezquieta, Begoña

Medicina clinica

2019 Volume 153, Issue 7, Page(s) 293–297

Title translation	Recomendaciones para la elaboración de informes genéticos de diagnóstico en el ámbito asistencial.
MeSH term(s)	Consent Forms/standards ; Family ; Genes, Dominant ; Genes, Recessive ; Genetic Counseling/standards ; Genetic Diseases, X-Linked/diagnosis ; Genetic Diseases, X-Linked/genetics ; Genetic Testing/methods ; Genetic Testing/standards ; Genetic Variation ; Health Records, Personal ; Humans ; Research Report/standards ; Sensitivity and Specificity
Language	Spanish
Publishing date	2019-08-05
Publishing country	Spain
Document type	Journal Article ; Practice Guideline
ZDB-ID	411607-0
ISSN	1578-8989 ; 0025-7753
ISSN (online)	1578-8989
ISSN	0025-7753
DOI	10.1016/j.medcli.2019.06.002
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