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Article ; Online: County-Level Factors Matter: The Role of Contextual Factors in Foster Youths' Extended Foster Care Participation and Human Capital Outcomes.

Ethan Park, Sunggeun / Okpych, Nathanael J / Harty, Justin S / Courtney, Mark E

2022 Volume 28, Issue 2, Page(s) 332–344

Abstract: Extended foster care (EFC) is an important policy that supports human capital attainment for foster youth transitioning to adult independence. Previous studies have examined youth- and policy-level factors' influence on EFC participation and human ... ...

Abstract	Extended foster care (EFC) is an important policy that supports human capital attainment for foster youth transitioning to adult independence. Previous studies have examined youth- and policy-level factors' influence on EFC participation and human capital outcomes (e.g., education, employment). Still, few studies have examined contextual factors (e.g., county characteristics). We explore how local contexts, or county-level attributes, influence youths' EFC participation and human capital outcomes (i.e., postsecondary education enrollment and earnings). We analyze two datasets from California Youth Transitions to Adulthood Study: survey data with rich youth-level information (
MeSH term(s)	Adult ; Child ; Adolescent ; Humans ; Young Adult ; Child, Foster ; Foster Home Care ; Educational Status ; Income ; Child Welfare
Language	English
Publishing date	2022-04-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1332193-6
ISSN	1552-6119 ; 1077-5595
ISSN (online)	1552-6119
ISSN	1077-5595
DOI	10.1177/10775595221088226
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Article ; Online: Study design and baseline profile for adults with type 2 diabetes in the once-weekly subcutaneous SEmaglutide randomized PRAgmatic (SEPRA) trial.

Buse, John B / Nordahl Christensen, Helene / Harty, Brian J / Mitchell, Julie / Soule, Benjamin P / Zacherle, Emily / Cziraky, Mark / Willey, Vincent J

BMJ open diabetes research & care

2023 Volume 11, Issue 3

MeSH term(s)	Male ; Humans ; Adult ; Middle Aged ; Aged ; Female ; Diabetes Mellitus, Type 2/drug therapy ; Hypoglycemic Agents/adverse effects ; Glycated Hemoglobin ; Metformin/therapeutic use ; Sodium-Glucose Transporter 2 Inhibitors/therapeutic use
Chemical Substances	Hypoglycemic Agents ; semaglutide (53AXN4NNHX) ; Glycated Hemoglobin ; Metformin (9100L32L2N) ; Sodium-Glucose Transporter 2 Inhibitors
Language	English
Publishing date	2023-04-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2732918-5
ISSN	2052-4897 ; 2052-4897
ISSN (online)	2052-4897
ISSN	2052-4897
DOI	10.1136/bmjdrc-2022-003206
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Article ; Online: Efficacy and safety of coronary computed tomography angiography in diagnosing coronary lesions in children.

Gould, Sharon W / Harty, M Patricia / Cartoski, Mark / Krishnan, Vijay / Givler, Nicole / Ostrowski, John / Tsuda, Takeshi

Cardiology in the young

2023 Volume 34, Issue 4, Page(s) 838–845

Abstract: Introduction: Identification of paediatric coronary artery abnormalities is challenging. We studied whether coronary artery CT angiography can be performed safely and reliably in children.: Materials: Retrospective analysis of consecutive coronary CT ...

Abstract	Introduction: Identification of paediatric coronary artery abnormalities is challenging. We studied whether coronary artery CT angiography can be performed safely and reliably in children. Materials: Retrospective analysis of consecutive coronary CT angiography scans was performed for image quality and estimated radiation dose. Both factors were assessed for correlation with electrocardiographic-gating technique that was protocoled on a case-by-case basis, radiation exposure parameters, image noise artefact parameters, heart rate, and heart rate variability. Results: Sixty scans were evaluated, of which 96.5% were diagnostic for main left and right coronaries and 91.3% were considered diagnostic for complete coronary arteries. Subjective image quality correlated significantly with lower heart rate, increasing patient age, and higher signal-to-noise ratio. Estimated radiation dose only correlated significantly with choice of electrocardiographic-gating technique with median doses as follows: 2.42 mSv for electrocardiographic-gating triggered high-pitch spiral technique, 5.37 mSv for prospectively triggered axial sequential technique, 3.92 mSv for retrospectively gated technique, and 5.64 mSv for studies which required multiple runs. Two scans were excluded for injection failure and one for protocol outside the study scope. Five non-diagnostic cases were attributed to breathing motion, scanning prior to peak contrast enhancement, or scan acquisition during the incorrect portion of the R-R interval. Conclusions: Diagnostic-quality coronary CT angiography can be performed reliably with a low estimated radiation exposure by tailoring each scan protocol to the patient's body habitus and heart rate. We propose coronary CT angiography is a safe and effective diagnostic modality for coronary artery abnormalities in children.
MeSH term(s)	Humans ; Child ; Computed Tomography Angiography/adverse effects ; Computed Tomography Angiography/methods ; Retrospective Studies ; Radiation Dosage ; Tomography, X-Ray Computed/methods ; Heart ; Coronary Artery Disease/diagnostic imaging ; Coronary Angiography/adverse effects ; Coronary Angiography/methods ; Heart Defects, Congenital ; Cardiac-Gated Imaging Techniques/methods
Language	English
Publishing date	2023-10-25
Publishing country	England
Document type	Journal Article
ZDB-ID	1078466-4
ISSN	1467-1107 ; 1047-9511
ISSN (online)	1467-1107
ISSN	1047-9511
DOI	10.1017/S1047951123003438
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Article: Parents of children with disabilities: A systematic review of parenting interventions and self-efficacy.

Hohlfeld, Ameer S J / Harty, Michal / Engel, Mark E

African journal of disability

2018 Volume 7, Page(s) 437

Abstract: Background: An increasing body of empirical evidence suggests that early intervention has positive outcomes for parents of children with neurodevelopmental disabilities. Parental self-efficacy has been used as an outcome measure in some empirical ... ...

Abstract	Background: An increasing body of empirical evidence suggests that early intervention has positive outcomes for parents of children with neurodevelopmental disabilities. Parental self-efficacy has been used as an outcome measure in some empirical studies; however, there is a lack of evidence of the impact of parent training programmes on parenting self-efficacy beliefs. Objectives: This systematic review sought to assess the effectiveness of parenting interventions to increase parental self-efficacy levels in parents of young children with neurodevelopmental disabilities. Method: We conducted a broad literature search, which included grey literature, such as dissertations and unpublished conference presentations, to identify all relevant prospective studies reporting on our study objective. Articles were selected for inclusion using predefined criteria and data were extracted onto a purposely designed data extraction form. Twenty-five articles met our search criteria. We extracted parenting self-efficacy scores before, and on, completion of parenting interventions and performed a meta-analysis using standardised mean difference. We also conducted a risk of bias assessment for all the included studies. Results: Parent training programmes resulted in a statistically significant increase in parental self-efficacy levels (standardised mean difference, 0.60 [95% confidence interval {CI}, 0.38-0.83]; I2, 74%) relative to baseline measurements. Parents of children younger than 5 years demonstrated the highest increase in levels of parental self-efficacy after parenting interventions. Furthermore, this review showed that psychologists and other healthcare practitioners are successfully able to implement training programmes that enhance parenting self-efficacy. Conclusion: Parent training programmes are effective in increasing parental self-efficacy in parents of children with neurodevelopmental disabilities.
Language	English
Publishing date	2018-10-17
Publishing country	South Africa
Document type	Journal Article ; Review
ZDB-ID	2733893-9
ISSN	2226-7220 ; 2223-9170
ISSN (online)	2226-7220
ISSN	2223-9170
DOI	10.4102/ajod.v7i0.437
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Book: Testing and test automation for mobile phone applications

Harty, Julian Mark Alistair

2014

Author's details	Julian Mark Alistair Harty
Language	English
Size	pages
Publisher	Chapman & Hall Crc
Publishing place	Place of publication not identified
Document type	Book
ISBN	1466594373 ; 9781466594371
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article ; Online: Catalytic properties of the metal ion variants of mandelate racemase reveal alterations in the apparent electrophilicity of the metal cofactor.

Harty, Matthew L / Sharma, Amar Nath / Bearne, Stephen L

Metallomics : integrated biometal science

2019 Volume 11, Issue 3, Page(s) 707–723

Abstract: ... by Mn2+, Co2+, or Ni2+, substitution by these metal ions does not markedly (<10-fold) alter the kinetic ... of the metal ion unlike the marked variation of the stability constants (K1) observed for M2+·BzH ...

Abstract	Mandalate racemase (MR) from Pseudomonas putida requires a divalent metal cation, usually Mg2+, to catalyse the interconversion of the enantiomers of mandelate. Although the active site Mg2+ may be replaced by Mn2+, Co2+, or Ni2+, substitution by these metal ions does not markedly (<10-fold) alter the kinetic parameters Kappm, kappcat, and (kcat/Km)app for the substrates (R)- and (S)-mandelate, and the alternative substrate (S)-trifluorolactate. Viscosity variation experiments with Mn2+-MR showed that the metal ion plays a role in the uniform binding of the transition states for enzyme-substrate association, the chemical step, and enzyme-product dissociation. Surprisingly, the competitive inhibition constants (Ki) for inhibition of each metalloenzyme variant by benzohydroxamate did not vary significantly with the identity of the metal ion unlike the marked variation of the stability constants (K1) observed for M2+·BzH complex formation in solution. A similar trend was observed for the inhibition of the metalloenzyme variants by F-, except for Mg2+-MR, which bound F- tighter than would be predicted based on the stability constants for formation of M2+·F- complexes in solution. Thus, the enzyme modifies the enatic state of the bound metal ion cofactor so that the apparent electrophilicity of Mg2+ is enhanced, while that of Ni2+ is attenuated, resulting in a levelling effect relative to the trends observed for the free metals in solution.
MeSH term(s)	Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Cations, Divalent/chemistry ; Cations, Divalent/metabolism ; Coenzymes/chemistry ; Coenzymes/metabolism ; Fluorides/chemistry ; Fluorides/metabolism ; Hydroxamic Acids/chemistry ; Hydroxamic Acids/metabolism ; Magnesium/chemistry ; Magnesium/metabolism ; Models, Molecular ; Nickel/chemistry ; Nickel/metabolism ; Protein Binding ; Pseudomonas putida/enzymology ; Racemases and Epimerases/chemistry ; Racemases and Epimerases/metabolism ; Thermodynamics
Chemical Substances	Bacterial Proteins ; Cations, Divalent ; Coenzymes ; Hydroxamic Acids ; Nickel (7OV03QG267) ; Racemases and Epimerases (EC 5.1.-) ; mandelate racemase (EC 5.1.2.2) ; Magnesium (I38ZP9992A) ; benzohydroxamic acid (K8YW73872D) ; Fluorides (Q80VPU408O)
Language	English
Publishing date	2019-03-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2474317-3
ISSN	1756-591X ; 1756-5901
ISSN (online)	1756-591X
ISSN	1756-5901
DOI	10.1039/c8mt00330k
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Angiomotin Counteracts the Negative Regulatory Effect of Host WWOX on Viral PPxY-Mediated Egress.

Liang, Jingjing / Ruthel, Gordon / Sagum, Cari A / Bedford, Mark T / Sidhu, Sachdev S / Sudol, Marius / Jaladanki, Chaitanya K / Fan, Hao / Freedman, Bruce D / Harty, Ronald N

Journal of virology

2021 Volume 95, Issue 8

Abstract: ... ...

Abstract	Filoviridae
Language	English
Publishing date	2021-02-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.00121-21
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Article ; Online: Ubiquitin Ligase SMURF2 Interacts with Filovirus VP40 and Promotes Egress of VP40 VLPs.

Shepley-McTaggart, Ariel / Schwoerer, Michael Patrick / Sagum, Cari A / Bedford, Mark T / Jaladanki, Chaitanya K / Fan, Hao / Cassel, Joel / Harty, Ronald N

Viruses

2021 Volume 13, Issue 2

Abstract: Filoviruses Ebola (EBOV) and Marburg (MARV) are devastating high-priority pathogens capable of causing explosive outbreaks with high human mortality rates. The matrix proteins of EBOV and MARV, as well as eVP40 and mVP40, respectively, are the key viral ... ...

Abstract	Filoviruses Ebola (EBOV) and Marburg (MARV) are devastating high-priority pathogens capable of causing explosive outbreaks with high human mortality rates. The matrix proteins of EBOV and MARV, as well as eVP40 and mVP40, respectively, are the key viral proteins that drive virus assembly and egress and can bud independently from cells in the form of virus-like particles (VLPs). The matrix proteins utilize proline-rich Late (L) domain motifs (e.g., PPxY) to hijack specific host proteins that contain WW domains, such as the HECT family E3 ligases, to facilitate the last step of virus-cell separation. We identified E3 ubiquitin ligase Smad Ubiquitin Regulatory Factor 2 (SMURF2) as a novel interactor with VP40 that positively regulates VP40 VLP release. Our results show that eVP40 and mVP40 interact with the three WW domains of SMURF2 via their PPxY motifs. We provide evidence that the eVP40-SMURF2 interaction is functional as the expression of SMURF2 positively regulates VLP egress, while siRNA knockdown of endogenous SMURF2 decreases VLP budding compared to controls. In sum, our identification of novel interactor SMURF2 adds to the growing list of identified host proteins that can regulate PPxY-mediated egress of VP40 VLPs. A more comprehensive understanding of the modular interplay between filovirus VP40 and host proteins may lead to the development of new therapies to combat these deadly infections.
MeSH term(s)	Amino Acid Motifs ; Animals ; Ebolavirus/chemistry ; Ebolavirus/genetics ; Ebolavirus/physiology ; Hemorrhagic Fever, Ebola/enzymology ; Hemorrhagic Fever, Ebola/genetics ; Hemorrhagic Fever, Ebola/virology ; Humans ; Marburg Virus Disease/enzymology ; Marburg Virus Disease/genetics ; Marburg Virus Disease/virology ; Marburgvirus/chemistry ; Marburgvirus/genetics ; Marburgvirus/physiology ; Protein Binding ; Ubiquitin-Protein Ligases/genetics ; Ubiquitin-Protein Ligases/metabolism ; Viral Matrix Proteins/chemistry ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/metabolism ; Virion/genetics ; Virion/physiology ; Virus Assembly ; Virus Release
Chemical Substances	VP40 protein, virus ; Viral Matrix Proteins ; SMURF2 protein, human (EC 2.3.2.26) ; Ubiquitin-Protein Ligases (EC 2.3.2.27)
Language	English
Publishing date	2021-02-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v13020288
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Article ; Online: A Digital Case-Finding Algorithm for Diagnosed but Untreated Hepatitis C: A Tool for Increasing Linkage to Treatment and Cure.

Wyatt, Brooke / Perumalswami, Ponni V / Mageras, Anna / Miller, Mark / Harty, Alyson / Ma, Ning / Bowman, Chip A / Collado, Francina / Jeon, Jihae / Paulino, Lismeiry / Dinani, Amreen / Dieterich, Douglas / Li, Li / Vandromme, Maxence / Branch, Andrea D

Hepatology (Baltimore, Md.)

2021 Volume 74, Issue 6, Page(s) 2974–2987

Abstract: Background and aims: Although chronic HCV infection increases mortality, thousands of patients remain diagnosed-but-untreated (DBU). We aimed to (1) develop a DBU phenotyping algorithm, (2) use it to facilitate case finding and linkage to care, and (3) ... ...

Abstract	Background and aims: Although chronic HCV infection increases mortality, thousands of patients remain diagnosed-but-untreated (DBU). We aimed to (1) develop a DBU phenotyping algorithm, (2) use it to facilitate case finding and linkage to care, and (3) identify barriers to successful treatment. Approach and results: We developed a phenotyping algorithm using Java and SQL and applied it to ~2.5 million EPIC electronic medical records (EMRs; data entered January 2003 to December 2017). Approximately 72,000 EMRs contained an HCV International Classification of Diseases code and/or diagnostic test. The algorithm classified 10,614 cases as DBU (HCV-RNA positive and alive). Its positive and negative predictive values were 88% and 97%, respectively, as determined by manual review of 500 EMRs randomly selected from the ~72,000. Navigators reviewed the charts of 6,187 algorithm-defined DBUs and they attempted to contact potential treatment candidates by phone. By June 2020, 30% (n = 1,862) had completed an HCV-related appointment. Outcomes analysis revealed that DBU patients enrolled in our care coordination program were more likely to complete treatment (72% [n = 219] vs. 54% [n = 256]; P < 0.001) and to have a verified sustained virological response (67% vs. 46%; P < 0.001) than other patients. Forty-eight percent (n = 2,992) of DBU patients could not be reached by phone, which was a major barrier to engagement. Nearly half of these patients had Fibrosis-4 scores ≥ 2.67, indicating significant fibrosis. Multivariable logistic regression showed that DBUs who could not be contacted were less likely to have private insurance than those who could (18% vs. 50%; P < 0.001). Conclusions: The digital DBU case-finding algorithm efficiently identified potential HCV treatment candidates, freeing resources for navigation and coordination. The algorithm is portable and accelerated HCV elimination when incorporated in our comprehensive program.
MeSH term(s)	Aged ; Algorithms ; Antiviral Agents/therapeutic use ; Electronic Health Records/statistics & numerical data ; Feasibility Studies ; Female ; Hepatitis C, Chronic/diagnosis ; Hepatitis C, Chronic/drug therapy ; Humans ; Information Storage and Retrieval/methods ; Male ; Middle Aged
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2021-07-29
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	604603-4
ISSN	1527-3350 ; 0270-9139
ISSN (online)	1527-3350
ISSN	0270-9139
DOI	10.1002/hep.32086
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Article ; Online: Modular mimicry and engagement of the Hippo pathway by Marburg virus VP40: Implications for filovirus biology and budding.

Han, Ziying / Dash, Shantoshini / Sagum, Cari A / Ruthel, Gordon / Jaladanki, Chaitanya K / Berry, Corbett T / Schwoerer, Michael P / Harty, Nina M / Freedman, Bruce D / Bedford, Mark T / Fan, Hao / Sidhu, Sachdev S / Sudol, Marius / Shtanko, Olena / Harty, Ronald N

PLoS pathogens

2020 Volume 16, Issue 1, Page(s) e1008231

Abstract: Ebola (EBOV) and Marburg (MARV) are members of the Filoviridae family, which continue to emerge and cause sporadic outbreaks of hemorrhagic fever with high mortality rates. Filoviruses utilize their VP40 matrix protein to drive virion assembly and ... ...

Abstract	Ebola (EBOV) and Marburg (MARV) are members of the Filoviridae family, which continue to emerge and cause sporadic outbreaks of hemorrhagic fever with high mortality rates. Filoviruses utilize their VP40 matrix protein to drive virion assembly and budding, in part, by recruitment of specific WW-domain-bearing host proteins via its conserved PPxY Late (L) domain motif. Here, we screened an array of 115 mammalian, bacterially expressed and purified WW-domains using a PPxY-containing peptide from MARV VP40 (mVP40) to identify novel host interactors. Using this unbiased approach, we identified Yes Associated Protein (YAP) and Transcriptional co-Activator with PDZ-binding motif (TAZ) as novel mVP40 PPxY interactors. YAP and TAZ function as downstream transcriptional effectors of the Hippo signaling pathway that regulates cell proliferation, migration and apoptosis. We demonstrate that ectopic expression of YAP or TAZ along with mVP40 leads to significant inhibition of budding of mVP40 VLPs in a WW-domain/PPxY dependent manner. Moreover, YAP colocalized with mVP40 in the cytoplasm, and inhibition of mVP40 VLP budding was more pronounced when YAP was localized predominantly in the cytoplasm rather than in the nucleus. A key regulator of YAP nuclear/cytoplasmic localization and function is angiomotin (Amot); a multi-PPxY containing protein that strongly interacts with YAP WW-domains. Interestingly, we found that expression of PPxY-containing Amot rescued mVP40 VLP egress from either YAP- or TAZ-mediated inhibition in a PPxY-dependent manner. Importantly, using a stable Amot-knockdown cell line, we found that expression of Amot was critical for efficient egress of mVP40 VLPs as well as egress and spread of authentic MARV in infected cell cultures. In sum, we identified novel negative (YAP/TAZ) and positive (Amot) regulators of MARV VP40-mediated egress, that likely function in part, via competition between host and viral PPxY motifs binding to modular host WW-domains. These findings not only impact our mechanistic understanding of virus budding and spread, but also may impact the development of new antiviral strategies.
MeSH term(s)	Binding Sites ; Cell Membrane/metabolism ; Filoviridae/physiology ; Gene Knockout Techniques ; HEK293 Cells ; Humans ; Intercellular Signaling Peptides and Proteins/metabolism ; Marburgvirus/physiology ; Microfilament Proteins/metabolism ; Models, Molecular ; Molecular Mimicry ; PDZ Domains ; Protein Domains ; Proto-Oncogene Proteins c-yes/metabolism ; Recombinant Fusion Proteins/metabolism ; Viral Matrix Proteins/physiology ; Virus Release
Chemical Substances	AMOT protein, human ; Intercellular Signaling Peptides and Proteins ; Microfilament Proteins ; Recombinant Fusion Proteins ; VP40 protein, virus ; Viral Matrix Proteins ; Proto-Oncogene Proteins c-yes (EC 2.7.10.2)
Language	English
Publishing date	2020-01-06
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1008231
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