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  1. Article: The DNA Methyltransferase DMAP1 is Required for Tissue Maintenance and Planarian Regeneration.

    Rojas, Salvador / Barghouth, Paul G / Karabinis, Peter / Oviedo, Néstor J

    bioRxiv : the preprint server for biology

    2024  

    Abstract: The precise regulation of transcription is required for embryonic development, adult tissue turnover, and regeneration. Epigenetic modifications play a crucial role in orchestrating and regulating the transcription of genes. These modifications are ... ...

    Abstract The precise regulation of transcription is required for embryonic development, adult tissue turnover, and regeneration. Epigenetic modifications play a crucial role in orchestrating and regulating the transcription of genes. These modifications are important in the transition of pluripotent stem cells and their progeny. Methylation, a key epigenetic modification, influences gene expression through changes in histone tails and direct DNA methylation. Work in different organisms has shown that the DNA methyltransferase-1-associated protein (DMAP1) may associate with other molecules to repress transcription through DNA methylation. Thus, DMAP1 is a versatile protein implicated in a myriad of events, including pluripotency maintenance, DNA damage repair, and tumor suppression. While DMAP1 has been extensively studied
    Language English
    Publishing date 2024-04-11
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.04.10.588909
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Whole planarian chromosome squash.

    Barghouth, Paul G / Oviedo, Néstor J

    STAR protocols

    2021  Volume 2, Issue 1, Page(s) 100257

    Abstract: Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic- ...

    Abstract Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic-orcein, a stain-fixative for chromosomes, this strategy is suitable for animals with friable tissues caused by drug treatment, radiation, and RNA interference phenotypes. The whole planarian squash method presented here is a minimally invasive procedure that facilitates simultaneous analysis of chromosomal integrity in control and experimental animals. For complete details on the use and execution of this protocol, please refer to Peiris et al. (2016).
    MeSH term(s) Animals ; Chromosomes/genetics ; Metaphase ; Planarians/genetics ; RNA Interference
    Language English
    Publishing date 2021-01-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2020.100257
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Measuring protein levels in planarians using western blotting.

    Ziman, Benjamin / Oviedo, Néstor J

    STAR protocols

    2021  Volume 2, Issue 1, Page(s) 100274

    Abstract: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in antibody ... ...

    Abstract In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in antibody availability and issues related to nonspecific expression. The use of western blotting can circumvent nonspecific expression, providing a dependable way to quantify proteins of interest. Here, we present a standardized, easily reproducible protocol with details on protein extractions of whole planarians and western blotting. For complete details on the use and execution of this protocol, please refer to Ziman et al. (2020a).
    MeSH term(s) Animals ; Blotting, Western ; Helminth Proteins/metabolism ; Planarians/metabolism ; Regeneration
    Chemical Substances Helminth Proteins
    Language English
    Publishing date 2021-01-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2020.100274
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Can neural signals override cellular decisions in the presence of DNA damage?

    Rojas, Salvador / Oviedo, Néstor J

    DNA repair

    2021  Volume 103, Page(s) 103127

    Abstract: Cells within an organism are in constant crosstalk with their surrounding environment. Short and long-range signals influence cellular behavior associated with division, differentiation, and death. This crosstalk among cells underlies tissue renewal to ... ...

    Abstract Cells within an organism are in constant crosstalk with their surrounding environment. Short and long-range signals influence cellular behavior associated with division, differentiation, and death. This crosstalk among cells underlies tissue renewal to guarantee faithful replacement of old or damaged cells over many years. Renewing tissues also offer recurrent opportunities for DNA damage and cellular transformation that tend to occur with aging. Most cells with extensive DNA damage have limited options such as halting cell cycle to repair DNA, undergo senescence, or programmed cell death. However, in some cases cells carrying toxic forms of DNA damage survive and proliferate. The underlying factors driving survival and proliferation of cells with DNA damage remain unknown. Here we discuss potential roles the nervous system may play in influencing the fate of cells with DNA damage. We present a brief survey highlighting the implications the nervous system has in regeneration, regulation of stem cells, modulation of the immune system, and its contribution to cancer progression. Finally, we propose the use of planarian flatworms as a convenient model organism to molecularly dissect the influence of neural signals over cellular fate regulation in the presence of DNA damage.
    MeSH term(s) Animals ; Apoptosis ; Cell Cycle ; Cellular Senescence ; DNA Damage ; Nervous System ; Nervous System Physiological Phenomena ; Planarians/genetics ; Planarians/physiology ; Stem Cells/physiology
    Language English
    Publishing date 2021-04-20
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2071608-4
    ISSN 1568-7856 ; 1568-7864
    ISSN (online) 1568-7856
    ISSN 1568-7864
    DOI 10.1016/j.dnarep.2021.103127
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Measuring protein levels in planarians using western blotting

    Benjamin Ziman / Néstor J. Oviedo

    STAR Protocols, Vol 2, Iss 1, Pp 100274- (2021)

    2021  

    Abstract: Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in ... ...

    Abstract Summary: In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in antibody availability and issues related to nonspecific expression. The use of western blotting can circumvent nonspecific expression, providing a dependable way to quantify proteins of interest. Here, we present a standardized, easily reproducible protocol with details on protein extractions of whole planarians and western blotting.For complete details on the use and execution of this protocol, please refer to Ziman et al. (2020a).
    Keywords Model organisms ; Molecular biology ; Protein biochemistry ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Can neural signals override cellular decisions in the presence of DNA damage?

    Rojas, Salvador / Oviedo, Néstor J.

    DNA Repair. 2021 July, v. 103

    2021  

    Abstract: Cells within an organism are in constant crosstalk with their surrounding environment. Short and long-range signals influence cellular behavior associated with division, differentiation, and death. This crosstalk among cells underlies tissue renewal to ... ...

    Abstract Cells within an organism are in constant crosstalk with their surrounding environment. Short and long-range signals influence cellular behavior associated with division, differentiation, and death. This crosstalk among cells underlies tissue renewal to guarantee faithful replacement of old or damaged cells over many years. Renewing tissues also offer recurrent opportunities for DNA damage and cellular transformation that tend to occur with aging. Most cells with extensive DNA damage have limited options such as halting cell cycle to repair DNA, undergo senescence, or programmed cell death. However, in some cases cells carrying toxic forms of DNA damage survive and proliferate. The underlying factors driving survival and proliferation of cells with DNA damage remain unknown. Here we discuss potential roles the nervous system may play in influencing the fate of cells with DNA damage. We present a brief survey highlighting the implications the nervous system has in regeneration, regulation of stem cells, modulation of the immune system, and its contribution to cancer progression. Finally, we propose the use of planarian flatworms as a convenient model organism to molecularly dissect the influence of neural signals over cellular fate regulation in the presence of DNA damage.
    Keywords DNA ; DNA damage ; DNA repair ; cell cycle ; death ; immune system ; neoplasm progression ; nervous system ; programmed cell death ; surveys ; toxicity
    Language English
    Dates of publication 2021-07
    Publishing place Elsevier BV
    Document type Article
    ZDB-ID 2071608-4
    ISSN 1568-7856 ; 1568-7864
    ISSN (online) 1568-7856
    ISSN 1568-7864
    DOI 10.1016/j.dnarep.2021.103127
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: A Planarian Model System to Study Host-Pathogen Interactions.

    Maciel, Eli Isael / Valle Arevalo, Ashley / Nobile, Clarissa J / Oviedo, Néstor J

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2680, Page(s) 231–244

    Abstract: This protocol is focused on using the recently established planarian infection model system to study host-pathogen interactions during fungal infection. Here, we describe in detail the infection of the planarian Schmidtea mediterranea with the human ... ...

    Abstract This protocol is focused on using the recently established planarian infection model system to study host-pathogen interactions during fungal infection. Here, we describe in detail the infection of the planarian Schmidtea mediterranea with the human fungal pathogen Candida albicans. This simple and reproducible model system allows for rapid visualization of tissue damage throughout different infection timepoints. We note that this model system has been optimized for use with C. albicans, but should also be applicable for use with other pathogens of interest.
    MeSH term(s) Animals ; Humans ; Planarians ; Candida albicans ; Host-Pathogen Interactions ; Models, Biological
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3275-8_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Non-traditional roles of immune cells in regeneration: an evolutionary perspective.

    Arinda, Beryl N / Innabi, Yacoub A / Grasis, Juris A / Oviedo, Néstor J

    Development (Cambridge, England)

    2022  Volume 149, Issue 8

    Abstract: Immune cells are known to engage in pathogen defense. However, emerging research has revealed additional roles for immune cells, which are independent of their function in the immune response. Here, we underscore the ability of cells outside of the ... ...

    Abstract Immune cells are known to engage in pathogen defense. However, emerging research has revealed additional roles for immune cells, which are independent of their function in the immune response. Here, we underscore the ability of cells outside of the adaptive immune system to respond to recurring infections through the lens of evolution and cellular memory. With this in mind, we then discuss the bidirectional crosstalk between the immune cells and stem cells and present examples where these interactions regulate tissue repair and regeneration. We conclude by suggesting that comprehensive analyses of the immune system may enable biomedical applications in stem cell biology and regenerative medicine.
    MeSH term(s) Immune System ; Immunity, Innate ; Regenerative Medicine ; Stem Cells ; Wound Healing
    Language English
    Publishing date 2022-05-03
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.199903
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Ub I Zs.183: Show issues Location:
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  9. Article ; Online: Whole planarian chromosome squash

    Paul G. Barghouth / Néstor J. Oviedo

    STAR Protocols, Vol 2, Iss 1, Pp 100257- (2021)

    2021  

    Abstract: Summary: Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination ... ...

    Abstract Summary: Whole planarian chromosome squash allows researchers to qualitatively analyze chromosome integrity. Treatment with colchicine is used to halt dividing cells within metaphase and does not require amputation or tissue puncturing. In combination with acetic-orcein, a stain-fixative for chromosomes, this strategy is suitable for animals with friable tissues caused by drug treatment, radiation, and RNA interference phenotypes. The whole planarian squash method presented here is a minimally invasive procedure that facilitates simultaneous analysis of chromosomal integrity in control and experimental animals.For complete details on the use and execution of this protocol, please refer to Peiris et al. (2016).
    Keywords Model organisms ; Molecular biology ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  10. Article ; Online: Direct Current Electric Stimulation Alters the Frequency and the Distribution of Mitotic Cells in Planarians.

    Davidian, Devon / Ziman, Benjamin / Escobar, Ariel L / Oviedo, Néstor J

    Bioelectricity

    2021  Volume 3, Issue 1, Page(s) 77–91

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2021-03-16
    Publishing country United States
    Document type Journal Article
    ISSN 2576-3113
    ISSN (online) 2576-3113
    DOI 10.1089/bioe.2020.0026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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